Restriction map for
pFN21AB9575
(Circular) (Six-base) MAPSORT of: pFN21AB9575.seq Check: 5494 from: 1 to: 6526
pFN21AB9575 6526 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4336 278
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2838 2871 3869 1937
Size: 901 33 998 4594
Fragments arranged by size:
4594 998 901 33
Acc65I G'GTAC_C
Cuts at: 3847 3847
Size: 6526
AclI AA'CG_TT
Cuts at: 1563 4653 5026 1563
Size: 3090 373 3063
Fragments arranged by size:
3090 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2499 2932 3485 3545 3824 4589 5973 6241
Size: 867 433 553 60 279 765 1384 268
Cuts at: 6241 1632
Size: 1917
Fragments arranged by size:
1917 1384 867 765 553 433 279 268
60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6329
AflIII A'CryG_T
Cuts at: 1203 5425 6402 1203
Size: 4222 977 1327
Fragments arranged by size:
4222 1327 977
AgeI A'CCGG_T
Cuts at: 5357 5357
Size: 6526
AhdI GACnn_n'nnGTC
Cuts at: 5255 5255
Size: 6526
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6526
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2250 2571 3252 1184
Size: 1066 321 681 4458
Fragments arranged by size:
4458 1066 681 321
AlwNI CAG_nnn'CTG
Cuts at: 3463 4269 5841 3463
Size: 806 1572 4148
Fragments arranged by size:
4148 1572 806
ApaLI G'TGCA_C
Cuts at: 3425 4583 5739 3425
Size: 1158 1156 4212
Fragments arranged by size:
4212 1158 1156
ApoI r'AATT_y
Cuts at: 1493 3668 3779 3835 4061 4213 6322 1493
Size: 2175 111 56 226 152 2109 1697
Fragments arranged by size:
2175 2109 1697 226 152 111 56
AseI AT'TA_AT
Cuts at: 160 1032 5080 160
Size: 872 4048 1606
Fragments arranged by size:
4048 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 2471 3851 1870
Size: 73 104 424 1380 4545
Fragments arranged by size:
4545 1380 424 104 73
BamHI G'GATC_C
Cuts at: 3594 3856 4223 3594
Size: 262 367 5897
Fragments arranged by size:
5897 367 262
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3847 5302 618
Size: 325 217 627 2060 1455 1842
Fragments arranged by size:
2060 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3450 3845 1391
Size: 2059 395 4072
Fragments arranged by size:
4072 2059 395
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2506 3130 3601 928
Size: 971 607 624 471 3853
Fragments arranged by size:
3853 971 624 607 471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2633 2667 4717 4751 1903
Size: 34 696 34 2050 34 3678
Fragments arranged by size:
3678 2050 696 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4418 5628 4418
Size: 1210 5316
BclI T'GATC_A
Cuts at: 1548 2286 1548
Size: 738 5788
BfrBI ATG'CAT
Cuts at: 3235 3235
Size: 6526
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3557 5137 136
Size: 107 122 71 631 605 1885 1580 1525
Fragments arranged by size:
1885 1580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6521 6521
Size: 6526
BlpI GC'TnA_GC
Cuts at: 3943 3943
Size: 6526
Bme1580I G_kGCm'C
Cuts at: 1790 3411 3429 4587 5743 1790
Size: 1621 18 1158 1156 2573
Fragments arranged by size:
2573 1621 1158 1156 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6526
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4257 5215 442
Size: 416 53 83 399 24 2840 958 1753
Fragments arranged by size:
2840 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6526
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2356 3035 3281 5186 1239
Size: 1117 679 246 1905 2579
Fragments arranged by size:
2579 1905 1117 679 246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4651 5516 5814 4651
Size: 865 298 5363
Fragments arranged by size:
5363 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3814 5189 882
Size: 617 2315 1375 2219
Fragments arranged by size:
2315 2219 1375 617
BsaAI yAC'GTr
Cuts at: 493 1204 3195 4317 493
Size: 711 1991 1122 2702
Fragments arranged by size:
2702 1991 1122 711
BsaBI GATnn'nnATC
Cuts at: 3719 6520 3719
Size: 2801 3725
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4333 4715 5350 275
Size: 53 83 186 936 2800 382 635 1451
Fragments arranged by size:
2800 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2030 3591 4959 5357 5631 5778 6189 2030
Size: 1561 1368 398 274 147 411 2367
Fragments arranged by size:
2367 1561 1368 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2576 2606 1178
Size: 30 165 30 1173 30 5098
Fragments arranged by size:
5098 1173 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 2777 3143 3800 1186
Size: 1588 3 366 657 3912
Fragments arranged by size:
3912 1588 657 366 3
BseYI C'CCAG_C
Cuts at: 2192 2903 3498 5729 6225 2192
Size: 711 595 2231 496 2493
Fragments arranged by size:
2493 2231 711 595 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2957 1163
Size: 573 1221 4732
Fragments arranged by size:
4732 1221 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3976 4737 5765 1540
Size: 275 186 1975 761 1028 2301
Fragments arranged by size:
2301 1975 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2118 3429 4587 4672 5743 2118
Size: 1311 1158 85 1071 2901
Fragments arranged by size:
2901 1311 1158 1071 85
BsmI GAATG_Cn'
Cuts at: 3288 4037 4130 3288
Size: 749 93 5684
Fragments arranged by size:
5684 749 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 3411 3429 3450 3845 4587 4672
Size: 399 328 1293 18 21 395 742 85
Cuts at: 4672 5743 1391
Size: 1071 2174
Fragments arranged by size:
2174 1293 1071 742 399 395 328 85
21 18
BspEI T'CCGG_A
Cuts at: 3591 6189 3591
Size: 2598 3928
BspHI T'CATG_A
Cuts at: 4415 6145 4415
Size: 1730 4796
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3881 844
Size: 3037 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2092 4413 1394
Size: 429 269 2321 3507
Fragments arranged by size:
3507 2321 429 269
BsrDI GCAATG_nn'
Cuts at: 65 1248 2484 2509 3249 5021 5195 65
Size: 1183 1236 25 740 1772 174 1396
Fragments arranged by size:
1772 1396 1236 1183 740 174 25
BsrFI r'CCGG_y
Cuts at: 1700 1810 5170 5357 1700
Size: 110 3360 187 2869
Fragments arranged by size:
3360 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6526
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6526
BssSI C'ACGA_G
Cuts at: 3632 4586 5598 3632
Size: 954 1012 4560
Fragments arranged by size:
4560 1012 954
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3241 794
Size: 2447 4079
BstBI TT'CG_AA
Cuts at: 5330 5330
Size: 6526
BstEII G'GTnAC_C
Cuts at: 1184 2885 3070 3344 1184
Size: 1701 185 274 4366
Fragments arranged by size:
4366 1701 274 185
BstXI CCAn_nnnn'nTGG
Cuts at: 3547 3547
Size: 6526
BstYI r'GATC_y
Cuts at: 1972 2218 2403 3594 3771 3856 4223 4608 4625
Size: 246 185 1191 177 85 367 385 17
Cuts at: 4625 6077 6163 6175 6521 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 246 185
177 86 85 17 12
BstZ17I GTA'TAC
Cuts at: 2872 2872
Size: 6526
Bsu36I CC'TnA_GG
Cuts at: 2071 2280 2071
Size: 209 6317
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5035
Fragments arranged by size:
5035 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3706 487
Size: 683 56 2480 3307
Fragments arranged by size:
3307 2480 683 56
BtsI GCAGTG_nn'
Cuts at: 4042 4836 4856 4042
Size: 794 20 5712
Fragments arranged by size:
5712 794 20
ClaI AT'CG_AT
Cuts at: 1554 2694 1554
Size: 1140 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2548 2583 529
Size: 35 1984 35 4472
Fragments arranged by size:
4472 1984 35 35
DraI TTT'AAA
Cuts at: 3830 3830
Size: 6526
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3353 3734 5533 809
Size: 507 2037 381 1799 1802
Fragments arranged by size:
2037 1802 1799 507 381
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3246 3549
Size: 54 1425 50 275 18 177 1239 303
Cuts at: 3549 3973 4862 6491 8
Size: 424 889 1629 43
Fragments arranged by size:
1629 1425 1239 889 424 303 275 177
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3973 1537
Size: 275 2161 4090
Fragments arranged by size:
4090 2161 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2627 3032 4456 1353
Size: 1274 405 1424 3423
Fragments arranged by size:
3423 1424 1274 405
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2957 5113 5487 5633 387
Size: 1064 138 1368 2156 374 146 1280
Fragments arranged by size:
2156 1368 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2356 2499 2932 3035 3281 3485 3545
Size: 393 724 143 433 103 246 204 60
Cuts at: 3545 3824 4589 5186 5973 6241 1239
Size: 279 765 597 787 268 1524
Fragments arranged by size:
1524 787 765 724 597 433 393 279
268 246 204 143 103 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2021 2021
Size: 6526
EcoO109I rG'GnC_Cy
Cuts at: 2230 2573 2230
Size: 343 6183
EcoRI G'AATT_C
Cuts at: 3835 4213 3835
Size: 378 6148
FspI TGC'GCA
Cuts at: 3332 5032 3332
Size: 1700 4826
HaeII r_GCGC'y
Cuts at: 1678 5673 1678
Size: 3995 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2144 2176 3097 3129 5167
Size: 32 135 32 572 32 921 32 2038
Cuts at: 5167 5199 5241 5273 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2038 921 572 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3691 3870 4116 669
Size: 988 281 1753 179 246 3079
Fragments arranged by size:
3079 1753 988 281 246 179
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6526
HpaI GTT'AAC
Cuts at: 4116 4116
Size: 6526
KpnI G_GTAC'C
Cuts at: 3851 3851
Size: 6526
MfeI C'AATT_G
Cuts at: 4125 4125
Size: 6526
MluI A'CGCG_T
Cuts at: 6402 6402
Size: 6526
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2844 2871 5640 5824 1305
Size: 1539 27 2769 184 2007
Fragments arranged by size:
2769 2007 1539 184 27
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3248 3551 10
Size: 54 1425 343 177 1239 303 2985
Fragments arranged by size:
2985 1425 1239 343 303 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2553 3008 3539 3545 4484 4843 5002
Size: 648 1387 455 531 6 939 359 159
Cuts at: 5002 518
Size: 2042
Fragments arranged by size:
2042 1387 939 648 531 455 359 159
6
MspA1I CmG'CkG
Cuts at: 1606 2147 2427 2736 3301 3463 3645 3942 4619
Size: 541 280 309 565 162 182 297 677
Cuts at: 4619 5767 6012 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 565 541 309 297 280
245 182 162
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5035
Fragments arranged by size:
5035 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6526
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6526
NotI GC'GGCC_GC
Cuts at: 3973 3973
Size: 6526
NsiI A_TGCA'T
Cuts at: 3237 3237
Size: 6526
NspI r_CATG'y
Cuts at: 1132 1229 3181 3239 3337 3884 5429 1132
Size: 97 1952 58 98 547 1545 2229
Fragments arranged by size:
2229 1952 1545 547 98 97 58
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6526
PciI A'CATG_T
Cuts at: 5425 5425
Size: 6526
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2498 2676 2796 1108
Size: 1390 178 120 4838
Fragments arranged by size:
4838 1390 178 120
PfoI T'CCnGG_A
Cuts at: 2406 3567 2406
Size: 1161 5365
PmeI GTTT'AAAC
Cuts at: 3830 3830
Size: 6526
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3572 3604 4595 4627 6108 6140 3572
Size: 32 991 32 1481 32 3958
Fragments arranged by size:
3958 1481 991 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2230 2573 2230
Size: 343 6183
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6526
PsiI TTA'TAA
Cuts at: 4096 4096
Size: 6526
PstI C_TGCA'G
Cuts at: 830 3459 3878 830
Size: 2629 419 3478
Fragments arranged by size:
3478 2629 419
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6526
PvuII CAG'CTG
Cuts at: 2427 3301 3463 2427
Size: 874 162 5490
Fragments arranged by size:
5490 874 162
SalI G'TCGA_C
Cuts at: 1936 3868 1936
Size: 1932 4594
SbfI CC_TGCA'GG
Cuts at: 3878 3878
Size: 6526
ScaI AGT'ACT
Cuts at: 4774 6486 4774
Size: 1712 4814
SfcI C'TryA_G
Cuts at: 826 1045 2354 2542 3402 3455 3874 5009 5690
Size: 219 1309 188 860 53 419 1135 681
Cuts at: 5690 5881 826
Size: 191 1471
Fragments arranged by size:
1471 1309 1135 860 681 419 219 191
188 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6526
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6526
SmaI CCC'GGG
Cuts at: 1872 3853 1872
Size: 1981 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4630 5531 5793 820
Size: 197 926 2687 901 262 1553
Fragments arranged by size:
2687 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4317 493
Size: 3824 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6526
SphI G_CATG'C
Cuts at: 1132 3239 3337 3884 1132
Size: 2107 98 547 3774
Fragments arranged by size:
3774 2107 547 98
SspI AAT'ATT
Cuts at: 5 52 4450 5
Size: 47 4398 2081
Fragments arranged by size:
4398 2081 47
StuI AGG'CCT
Cuts at: 1517 2016 1517
Size: 499 6027
StyI C'CwwG_G
Cuts at: 513 1065 2004 2465 2580 3732 3965 513
Size: 552 939 461 115 1152 233 3074
Fragments arranged by size:
3074 1152 939 552 461 233 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4553 4570 4723 4908 5353 325
Size: 1255 2973 17 153 185 445 1498
Fragments arranged by size:
2973 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3484 4772 6484
Size: 274 80 33 51 1443 1507 1288 1712
Cuts at: 6484 96
Size: 138
Fragments arranged by size:
1712 1507 1443 1288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2203 2235 6486 6518 2203
Size: 32 4251 32 2211
Fragments arranged by size:
4251 2211 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6526
XbaI T'CTAG_A
Cuts at: 3862 3862
Size: 6526
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3010 3544 3010
Size: 534 5992
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6526
XmaI C'CCGG_G
Cuts at: 1870 3851 1870
Size: 1981 4545
XmnI GAAnn'nnTTC
Cuts at: 2026 4655 2026
Size: 2629 3897
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4334 276
Size: 53 83 186 936 2800 2468
Fragments arranged by size:
2800 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI