Restriction map for
pFN21AB9564
(Circular) (Six-base) MAPSORT of: pFN21AB9564.seq Check: 3335 from: 1 to: 5434
pFN21AB9564 5434 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3244 278
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2427 2777 1937
Size: 490 350 4594
Fragments arranged by size:
4594 490 350
Acc65I G'GTAC_C
Cuts at: 2755 2755
Size: 5434
AclI AA'CG_TT
Cuts at: 1563 3561 3934 1563
Size: 1998 373 3063
Fragments arranged by size:
3063 1998 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 2513 3497 4881 5149 1632
Size: 755 126 984 1384 268 1917
Fragments arranged by size:
1917 1384 984 755 268 126
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5237
AflIII A'CryG_T
Cuts at: 1203 4333 5310 1203
Size: 3130 977 1327
Fragments arranged by size:
3130 1327 977
AgeI A'CCGG_T
Cuts at: 4265 4265
Size: 5434
AhdI GACnn_n'nnGTC
Cuts at: 4163 4163
Size: 5434
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5434
AlwNI CAG_nnn'CTG
Cuts at: 2323 2365 3177 4749 2323
Size: 42 812 1572 3008
Fragments arranged by size:
3008 1572 812 42
ApaI G_GGCC'C
Cuts at: 2419 2563 2635 2716 2419
Size: 144 72 81 5137
Fragments arranged by size:
5137 144 81 72
ApaLI G'TGCA_C
Cuts at: 3491 4647 3491
Size: 1156 4278
ApoI r'AATT_y
Cuts at: 1493 2743 2969 3121 5230 1493
Size: 1250 226 152 2109 1697
Fragments arranged by size:
2109 1697 1250 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3988 160
Size: 872 2956 1606
Fragments arranged by size:
2956 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
AvaI C'yCGr_G
Cuts at: 1870 1943 2556 2619 2639 2759 1870
Size: 73 613 63 20 120 4545
Fragments arranged by size:
4545 613 120 73 63 20
BamHI G'GATC_C
Cuts at: 2478 2764 3131 2478
Size: 286 367 4781
Fragments arranged by size:
4781 367 286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2539 2688 2755 4210 618
Size: 325 217 627 752 149 67 1455 1842
Fragments arranged by size:
1842 1455 752 627 325 217 149 67
BanII G_rGCy'C
Cuts at: 1391 2419 2563 2635 2716 2753 1391
Size: 1028 144 72 81 37 4072
Fragments arranged by size:
4072 1028 144 81 72 37
BbeI G_GCGC'C
Cuts at: 2692 2692
Size: 5434
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2235 928
Size: 971 336 4127
Fragments arranged by size:
4127 971 336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3625 3659 1903
Size: 34 1688 34 3678
Fragments arranged by size:
3678 1688 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3326 4536 3326
Size: 1210 4224
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5434
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2630 2711 4045
Size: 107 122 71 631 605 958 81 1334
Cuts at: 4045 136
Size: 1525
Fragments arranged by size:
1525 1334 958 631 605 122 107 81
71
BglII A'GATC_T
Cuts at: 5429 5429
Size: 5434
BlpI GC'TnA_GC
Cuts at: 2372 2600 2851 2372
Size: 228 251 4955
Fragments arranged by size:
4955 251 228
Bme1580I G_kGCm'C
Cuts at: 1790 2419 2563 2635 2716 3495 4651 1790
Size: 629 144 72 81 779 1156 2573
Fragments arranged by size:
2573 1156 779 629 144 81 72
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5434
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2098 2147 3165
Size: 416 53 83 399 24 681 49 1018
Cuts at: 3165 4123 442
Size: 958 1753
Fragments arranged by size:
1753 1018 958 681 416 399 83 53
49 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5434
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 4094 1239
Size: 760 2095 2579
Fragments arranged by size:
2579 2095 760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 4424 4722 3559
Size: 865 298 4271
Fragments arranged by size:
4271 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 4097 882
Size: 617 714 1884 2219
Fragments arranged by size:
2219 1884 714 617
BsaAI yAC'GTr
Cuts at: 493 1204 3225 493
Size: 711 2021 2702
Fragments arranged by size:
2702 2021 711
BsaBI GATnn'nnATC
Cuts at: 5428 5428
Size: 5434
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2689 3241 3623 4258
Size: 53 83 186 936 1156 552 382 635
Cuts at: 4258 275
Size: 1451
Fragments arranged by size:
1451 1156 936 635 552 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3867 4265 4539 4686 5097 3867
Size: 398 274 147 411 4204
Fragments arranged by size:
4204 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5209
Fragments arranged by size:
5209 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2438 2690 2741 2744 1186
Size: 889 363 252 51 3 3876
Fragments arranged by size:
3876 889 363 252 51 3
BseYI C'CCAG_C
Cuts at: 2470 4637 5133 2470
Size: 2167 496 2771
Fragments arranged by size:
2771 2167 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4861
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2884 3645 4673 1540
Size: 275 186 883 761 1028 2301
Fragments arranged by size:
2301 1028 883 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3495 3580 4651 3495
Size: 85 1071 4278
Fragments arranged by size:
4278 1071 85
BsmI GAATG_Cn'
Cuts at: 2332 2945 3038 2332
Size: 613 93 4728
Fragments arranged by size:
4728 613 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2419 2563 2635 2716 2753 3495 3580
Size: 399 629 144 72 81 37 742 85
Cuts at: 3580 4651 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 629 399 144 85 81
72 37
BspEI T'CCGG_A
Cuts at: 5097 5097
Size: 5434
BspHI T'CATG_A
Cuts at: 3323 5053 3323
Size: 1730 3704
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2789 844
Size: 1945 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2702 3321 1394
Size: 429 879 619 3507
Fragments arranged by size:
3507 879 619 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3929 4103 65
Size: 1183 2681 174 1396
Fragments arranged by size:
2681 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2513 2604 4078 4265 1700
Size: 110 703 91 1474 187 2869
Fragments arranged by size:
2869 1474 703 187 110 91
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5434
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5434
BssSI C'ACGA_G
Cuts at: 3494 4506 3494
Size: 1012 4422
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5434
BstBI TT'CG_AA
Cuts at: 4238 4238
Size: 5434
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5434
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2230 2284 2062
Size: 168 54 5212
Fragments arranged by size:
5212 168 54
BstYI r'GATC_y
Cuts at: 1972 2478 2764 3131 3516 3533 4985 5071 5083
Size: 506 286 367 385 17 1452 86 12
Cuts at: 5083 5429 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 506 385 367 346 286 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2030 513
Size: 552 939 26 3917
Fragments arranged by size:
3917 939 552 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4695
Fragments arranged by size:
4695 683 56
BtsI GCAGTG_nn'
Cuts at: 2725 2950 3744 3764 2725
Size: 225 794 20 4395
Fragments arranged by size:
4395 794 225 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5434
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2240 2275 529
Size: 35 1676 35 3688
Fragments arranged by size:
3688 1676 35 35
DraI TTT'AAA
Cuts at: 2738 2738
Size: 5434
DraIII CAC_nnn'GTG
Cuts at: 2233 2233
Size: 5434
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4441 809
Size: 507 3125 1802
Fragments arranged by size:
3125 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2380 2511 2703
Size: 54 1425 50 275 18 550 131 192
Cuts at: 2703 2881 3770 5399 8
Size: 178 889 1629 43
Fragments arranged by size:
1629 1425 889 550 275 192 178 131
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2881 1537
Size: 275 1069 4090
Fragments arranged by size:
4090 1069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2135 3364 1353
Size: 782 1229 3423
Fragments arranged by size:
3423 1229 782
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2491 4021 4395 4541 387
Size: 1064 138 902 1530 374 146 1280
Fragments arranged by size:
1530 1280 1064 902 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2387 2513 3497 4094 4881 5149
Size: 393 367 388 126 984 597 787 268
Cuts at: 5149 1239
Size: 1524
Fragments arranged by size:
1524 984 787 597 393 388 367 268
126
EcoNI CCTnn'n_nnAGG
Cuts at: 2357 2357
Size: 5434
EcoO109I rG'GnC_Cy
Cuts at: 2362 2415 2416 2488 2560 2631 2712 2362
Size: 53 1 72 72 71 81 5084
Fragments arranged by size:
5084 81 72 72 71 53 1
EcoRI G'AATT_C
Cuts at: 2743 3121 2743
Size: 378 5056
FspI TGC'GCA
Cuts at: 3940 3940
Size: 5434
HaeII r_GCGC'y
Cuts at: 1678 2692 4581 1678
Size: 1014 1889 2531
Fragments arranged by size:
2531 1889 1014
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2231 2263 4075 4107 4149
Size: 32 135 32 659 32 1812 32 42
Cuts at: 4149 4181 1373
Size: 32 2626
Fragments arranged by size:
2626 1812 659 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2778 3024 669
Size: 988 281 840 246 3079
Fragments arranged by size:
3079 988 840 281 246
HindIII A'AGCT_T
Cuts at: 748 2495 748
Size: 1747 3687
HpaI GTT'AAC
Cuts at: 3024 3024
Size: 5434
KasI G'GCGC_C
Cuts at: 2688 2688
Size: 5434
KpnI G_GTAC'C
Cuts at: 2759 2759
Size: 5434
MfeI C'AATT_G
Cuts at: 3033 3033
Size: 5434
MluI A'CGCG_T
Cuts at: 5310 5310
Size: 5434
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4548 4732 1305
Size: 3243 184 2007
Fragments arranged by size:
3243 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2382 10
Size: 54 1425 343 550 3062
Fragments arranged by size:
3062 1425 550 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 2282 3392 3751 3910 518
Size: 648 894 222 1110 359 159 2042
Fragments arranged by size:
2042 1110 894 648 359 222 159
MspA1I CmG'CkG
Cuts at: 1606 2320 2850 3527 4675 4920 1606
Size: 714 530 677 1148 245 2120
Fragments arranged by size:
2120 1148 714 677 530 245
NaeI GCC'GGC
Cuts at: 2515 2606 2515
Size: 91 5343
NarI GG'CG_CC
Cuts at: 2689 2689
Size: 5434
NcoI C'CATG_G
Cuts at: 513 1065 2004 2030 513
Size: 552 939 26 3917
Fragments arranged by size:
3917 939 552 26
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5434
NgoMIV G'CCGG_C
Cuts at: 2513 2604 2513
Size: 91 5343
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5434
NotI GC'GGCC_GC
Cuts at: 2881 2881
Size: 5434
NspI r_CATG'y
Cuts at: 1132 1229 2045 2792 4337 1132
Size: 97 816 747 1545 2229
Fragments arranged by size:
2229 1545 816 747 97
PasI CC'CwG_GG
Cuts at: 1348 2359 1348
Size: 1011 4423
PciI A'CATG_T
Cuts at: 4333 4333
Size: 5434
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5434
PmeI GTTT'AAAC
Cuts at: 2738 2738
Size: 5434
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3503 3535 5016 5048 3503
Size: 32 1481 32 3889
Fragments arranged by size:
3889 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5434
PsiI TTA'TAA
Cuts at: 3004 3004
Size: 5434
PspOMI G'GGCC_C
Cuts at: 2415 2559 2631 2712 2415
Size: 144 72 81 5137
Fragments arranged by size:
5137 144 81 72
PspXI vC'TCGA_Gb
Cuts at: 2639 2639
Size: 5434
PstI C_TGCA'G
Cuts at: 830 2786 830
Size: 1956 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5434
PvuII CAG'CTG
Cuts at: 2320 2320
Size: 5434
SalI G'TCGA_C
Cuts at: 1936 2776 1936
Size: 840 4594
SbfI CC_TGCA'GG
Cuts at: 2786 2786
Size: 5434
ScaI AGT'ACT
Cuts at: 3682 5394 3682
Size: 1712 3722
SexAI A'CCwGG_T
Cuts at: 2229 2229
Size: 5434
SfcI C'TryA_G
Cuts at: 826 1045 2782 3917 4598 4789 826
Size: 219 1737 1135 681 191 1471
Fragments arranged by size:
1737 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2711 2711
Size: 5434
SfoI GGC'GCC
Cuts at: 2690 2690
Size: 5434
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5434
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5434
SmaI CCC'GGG
Cuts at: 1872 2558 2761 1872
Size: 686 203 4545
Fragments arranged by size:
4545 686 203
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 3538 4439 4701 820
Size: 197 926 696 899 901 262 1553
Fragments arranged by size:
1553 926 901 899 696 262 197
SnaBI TAC'GTA
Cuts at: 493 3225 493
Size: 2732 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5434
SphI G_CATG'C
Cuts at: 1132 2792 1132
Size: 1660 3774
SspI AAT'ATT
Cuts at: 5 52 3358 5
Size: 47 3306 2081
Fragments arranged by size:
3306 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5434
StyI C'CwwG_G
Cuts at: 513 1065 2004 2030 2331 2646 2706 2873 513
Size: 552 939 26 301 315 60 167 3074
Fragments arranged by size:
3074 939 552 315 301 167 60 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3461 3478 3631 3816 4261 325
Size: 1255 1881 17 153 185 445 1498
Fragments arranged by size:
1881 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3680 5392 96
Size: 274 80 33 51 1443 1703 1712 138
Fragments arranged by size:
1712 1703 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2028 2060 5394 5426 2028
Size: 32 3334 32 2036
Fragments arranged by size:
3334 2036 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5434
XbaI T'CTAG_A
Cuts at: 2770 2770
Size: 5434
XhoI C'TCGA_G
Cuts at: 1943 2639 1943
Size: 696 4738
XmaI C'CCGG_G
Cuts at: 1870 2556 2759 1870
Size: 686 203 4545
Fragments arranged by size:
4545 686 203
XmnI GAAnn'nnTTC
Cuts at: 3563 3563
Size: 5434
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3242 276
Size: 53 83 186 936 1708 2468
Fragments arranged by size:
2468 1708 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PmlI
PpuMI PsrI RsrII SacI SacII SanDI SapI SrfI
SwaI XcmI