Restriction map for
pFN21AB9563
(Circular) (Six-base) MAPSORT of: pFN21AB9563.seq Check: 8448 from: 1 to: 11671
pFN21AB9563 11671 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2790 4145 4285 5311 5824 6181 8147 8408 2790
Size: 1355 140 1026 513 357 1966 261 6053
Fragments arranged by size:
6053 1966 1355 1026 513 357 261 140
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8775 9481 278
Size: 53 83 186 936 7239 706 2468
Fragments arranged by size:
7239 2468 936 706 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4917 6534 6957 9014 1937
Size: 2980 1617 423 2057 4594
Fragments arranged by size:
4594 2980 2057 1617 423
Acc65I G'GTAC_C
Cuts at: 2810 4230 8992 2810
Size: 1420 4762 5489
Fragments arranged by size:
5489 4762 1420
AclI AA'CG_TT
Cuts at: 1563 9798 10171 1563
Size: 8235 373 3063
Fragments arranged by size:
8235 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2604 3986 5119 5741 6082 6776 7987 8059
Size: 972 1382 1133 622 341 694 1211 72
Cuts at: 8059 9734 11118 11386 1632
Size: 1675 1384 268 1917
Fragments arranged by size:
1917 1675 1384 1382 1211 1133 972 694
622 341 268 72
AfeI AGC'GCT
Cuts at: 7229 7229
Size: 11671
AflII C'TTAA_G
Cuts at: 820 1017 8948 820
Size: 197 7931 3543
Fragments arranged by size:
7931 3543 197
AflIII A'CryG_T
Cuts at: 1203 8667 10570 11547 1203
Size: 7464 1903 977 1327
Fragments arranged by size:
7464 1903 1327 977
AgeI A'CCGG_T
Cuts at: 10502 10502
Size: 11671
AhdI GACnn_n'nnGTC
Cuts at: 5534 6517 10400 5534
Size: 983 3883 6805
Fragments arranged by size:
6805 3883 983
AleI CACnn'nnGTG
Cuts at: 1166 2339 2829 3897 4683 4871 5447 6593 7371
Size: 1173 490 1068 786 188 576 1146 778
Cuts at: 7371 7404 8867 1166
Size: 33 1463 3970
Fragments arranged by size:
3970 1463 1173 1146 1068 786 778 576
490 188 33
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3157 4031 5688 6611 1184
Size: 1973 874 1657 923 6244
Fragments arranged by size:
6244 1973 1657 923 874
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3905 3937 5367 5399 3905
Size: 32 1430 32 10177
Fragments arranged by size:
10177 1430 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3298 3409 5297 5578 6196 6772 6842 6856 7598
Size: 111 1888 281 618 576 70 14 742
Cuts at: 7598 8782 9414 10986 3298
Size: 1184 632 1572 3983
Fragments arranged by size:
3983 1888 1572 1184 742 632 618 576
281 111 70 14
ApaI G_GGCC'C
Cuts at: 2631 4123 5708 6159 6881 7567 7674 8407 8886
Size: 1492 1585 451 722 686 107 733 479
Cuts at: 8886 2631
Size: 5416
Fragments arranged by size:
5416 1585 1492 733 722 686 479 451
107
ApaLI G'TGCA_C
Cuts at: 5285 5663 9728 10884 5285
Size: 378 4065 1156 6072
Fragments arranged by size:
6072 4065 1156 378
ApoI r'AATT_y
Cuts at: 1493 8980 9206 9358 11467 1493
Size: 7487 226 152 2109 1697
Fragments arranged by size:
7487 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10225 160
Size: 872 9193 1606
Fragments arranged by size:
9193 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11671
AvaI C'yCGr_G
Cuts at: 1870 1943 2975 3423 3446 3460 3582 5094 5149
Size: 73 1032 448 23 14 122 1512 55
Cuts at: 5149 6035 6995 7053 7151 7536 7560 8298 8315
Size: 886 960 58 98 385 24 738 17
Cuts at: 8315 8540 8996 1870
Size: 225 456 4545
Fragments arranged by size:
4545 1512 1032 960 886 738 456 448
385 225 122 98 73 58 55 24
23 17 14
BamHI G'GATC_C
Cuts at: 5310 9001 9368 5310
Size: 3691 367 7613
Fragments arranged by size:
7613 3691 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2713 2810 3586 3791
Size: 325 217 627 335 591 97 776 205
Cuts at: 3791 4230 4729 5432 5882 6580 7379 7627 7846
Size: 439 499 703 450 698 799 248 219
Cuts at: 7846 7858 8177 8992 10447 618
Size: 12 319 815 1455 1842
Fragments arranged by size:
1842 1455 815 799 776 703 698 627
591 499 450 439 335 325 319 248
219 217 205 97 12
BanII G_rGCy'C
Cuts at: 1391 2419 2442 2631 3447 3469 4123 5226 5708
Size: 1028 23 189 816 22 654 1103 482
Cuts at: 5708 5846 6159 6220 6633 6881 7002 7032 7302
Size: 138 313 61 413 248 121 30 270
Cuts at: 7302 7567 7674 8140 8316 8407 8625 8886 8990
Size: 265 107 466 176 91 218 261 104
Cuts at: 8990 1391
Size: 4072
Fragments arranged by size:
4072 1103 1028 816 654 482 466 413
313 270 265 261 248 218 189 176
138 121 107 104 91 61 30 23
22
BbeI G_GCGC'C
Cuts at: 5886 6584 5886
Size: 698 10973
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3078 4899 5014 5743 928
Size: 971 1179 1821 115 729 6856
Fragments arranged by size:
6856 1821 1179 971 729 115
BbvCI CC'TCA_GC
Cuts at: 2285 2401 2942 5618 6182 6254 7503 7646 2285
Size: 116 541 2676 564 72 1249 143 6310
Fragments arranged by size:
6310 2676 1249 564 541 143 116 72
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 9862 9896 1903
Size: 34 50 34 7841 34 3678
Fragments arranged by size:
7841 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9563 10773 9563
Size: 1210 10461
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11671
BfrBI ATG'CAT
Cuts at: 8108 8108
Size: 11671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2729 3215 3724
Size: 107 122 71 631 605 1057 486 509
Cuts at: 3724 4190 4604 5230 5610 5804 7439 7724 10282
Size: 466 414 626 380 194 1635 285 2558
Cuts at: 10282 136
Size: 1525
Fragments arranged by size:
2558 1635 1525 1057 631 626 605 509
486 466 414 380 285 194 122 107
71
BglII A'GATC_T
Cuts at: 5742 11666 5742
Size: 5924 5747
BlpI GC'TnA_GC
Cuts at: 3387 7760 9088 3387
Size: 4373 1328 5970
Fragments arranged by size:
5970 4373 1328
Bme1580I G_kGCm'C
Cuts at: 1790 2456 2631 2663 3335 3433 3591 4123 4520
Size: 666 175 32 672 98 158 532 397
Cuts at: 4520 5289 5435 5667 5708 6159 6590 6881 7382
Size: 769 146 232 41 451 431 291 501
Cuts at: 7382 7411 7552 7567 7630 7674 7849 7863 8298
Size: 29 141 15 63 44 175 14 435
Cuts at: 8298 8407 8449 8886 9732 10888 1790
Size: 109 42 437 846 1156 2573
Fragments arranged by size:
2573 1156 846 769 672 666 532 501
451 437 435 431 397 291 232 175
175 158 146 141 109 98 63 44
42 41 32 29 15 14
BmgBI CAC'GTC
Cuts at: 1318 2175 3499 5128 5506 7060 1318
Size: 857 1324 1629 378 1554 5929
Fragments arranged by size:
5929 1629 1554 1324 857 378
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2466 4125 4325
Size: 416 53 83 399 24 1049 1659 200
Cuts at: 4325 7954 8974 9402 10360 442
Size: 3629 1020 428 958 1753
Fragments arranged by size:
3629 1753 1659 1049 1020 958 428 416
399 200 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11671
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 3562 2957
Size: 605 11066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2283 2972 2993 3170 3584 4307 4391
Size: 899 145 689 21 177 414 723 84
Cuts at: 4391 4445 4457 5378 5386 5810 6007 6077 6218
Size: 54 12 921 8 424 197 70 141
Cuts at: 6218 6289 6814 7016 7400 7874 8282 8399 8456
Size: 71 525 202 384 474 408 117 57
Cuts at: 8456 8874 10331 1239
Size: 418 1457 2579
Fragments arranged by size:
2579 1457 921 899 723 689 525 474
424 418 414 408 384 202 197 177
145 141 117 84 71 70 57 54
21 12 8
Bpu10I CC'TnA_GC
Cuts at: 2285 2401 2942 3240 3255 4235 4313 5618 6182
Size: 116 541 298 15 980 78 1305 564
Cuts at: 6182 6254 7503 7646 8619 8648 2285
Size: 72 1249 143 973 29 5308
Fragments arranged by size:
5308 1305 1249 980 973 564 541 298
143 116 78 72 29 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3365 5403 6225 8378 8678 9796 10661 10959 3365
Size: 2038 822 2153 300 1118 865 298 4077
Fragments arranged by size:
4077 2153 2038 1118 865 822 300 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3816 4064 4397 4931 7145 8816 10334
Size: 617 2317 248 333 534 2214 1671 1518
Cuts at: 10334 882
Size: 2219
Fragments arranged by size:
2317 2219 2214 1671 1518 617 534 333
248
BsaAI yAC'GTr
Cuts at: 493 1204 4595 6301 9462 493
Size: 711 3391 1706 3161 2702
Fragments arranged by size:
3391 3161 2702 1706 711
BsaBI GATnn'nnATC
Cuts at: 11665 11665
Size: 11671
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2248 5163 5883 6042
Size: 53 83 186 936 715 2915 720 159
Cuts at: 6042 6581 8454 8772 9478 9860 10495 275
Size: 539 1873 318 706 382 635 1451
Fragments arranged by size:
2915 1873 1451 936 720 715 706 635
539 382 318 186 159 83 53
BsaWI w'CCGG_w
Cuts at: 10104 10502 10776 10923 11334 10104
Size: 398 274 147 411 10441
Fragments arranged by size:
10441 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3146 3176 5367 5397 5954
Size: 30 165 30 1743 30 2191 30 557
Cuts at: 5954 5984 7595 7625 8042 8072 8691 8721 1178
Size: 30 1611 30 417 30 619 30 4128
Fragments arranged by size:
4128 2191 1743 1611 619 557 417 165
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 2389 2534 2949 3167 3170 3173 3787
Size: 1108 95 145 415 218 3 3 614
Cuts at: 3787 4454 4712 5021 5183 6511 6737 7310 7870
Size: 667 258 309 162 1328 226 573 560
Cuts at: 7870 7937 8047 8050 1186
Size: 67 110 3 4807
Fragments arranged by size:
4807 1328 1108 667 614 573 560 415
309 258 226 218 162 145 110 95
67 3 3 3
BseYI C'CCAG_C
Cuts at: 2178 2212 2361 3058 3291 3721 4395 5166 5523
Size: 34 149 697 233 430 674 771 357
Cuts at: 5523 5568 5903 6207 6551 6615 6849 6882 6906
Size: 45 335 304 344 64 234 33 24
Cuts at: 6906 6962 7107 7115 7224 7268 7310 7335 7383
Size: 56 145 8 109 44 42 25 48
Cuts at: 7383 7566 8371 10874 11370 2178
Size: 183 805 2503 496 2479
Fragments arranged by size:
2503 2479 805 771 697 674 496 430
357 344 335 304 234 233 183 149
145 109 64 56 48 45 44 42
34 33 25 24 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3380 3891 4313 4364 5084 5384 5939
Size: 573 1644 511 422 51 720 300 555
Cuts at: 5939 5951 7856 1163
Size: 12 1905 4978
Fragments arranged by size:
4978 1905 1644 720 573 555 511 422
300 51 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3846 3986 5448 9121 9882 10910
Size: 275 186 1845 140 1462 3673 761 1028
Cuts at: 10910 1540
Size: 2301
Fragments arranged by size:
3673 2301 1845 1462 1028 761 275 186
140
BsiHKAI G_wGCw'C
Cuts at: 3447 4241 5289 5454 5667 6031 6503 6699 6747
Size: 794 1048 165 213 364 472 196 48
Cuts at: 6747 7032 7248 9732 9817 10888 3447
Size: 285 216 2484 85 1071 4230
Fragments arranged by size:
4230 2484 1071 1048 794 472 364 285
216 213 196 165 85 48
BsmI GAATG_Cn'
Cuts at: 9182 9275 9182
Size: 93 11578
BsmBI CGTCTCn'nnnn_
Cuts at: 2300 3088 2300
Size: 788 10883
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2419 2442 2456 2631 2663 3335 3433
Size: 399 629 23 14 175 32 672 98
Cuts at: 3433 3447 3469 3591 4123 4241 4520 5226 5289
Size: 14 22 122 532 118 279 706 63
Cuts at: 5289 5435 5454 5667 5708 5846 6031 6159 6220
Size: 146 19 213 41 138 185 128 61
Cuts at: 6220 6503 6590 6633 6699 6747 6881 7002 7032
Size: 283 87 43 66 48 134 121 30
Cuts at: 7032 7248 7302 7382 7411 7552 7567 7630 7674
Size: 216 54 80 29 141 15 63 44
Cuts at: 7674 7849 7863 8140 8298 8316 8407 8449 8625
Size: 175 14 277 158 18 91 42 176
Cuts at: 8625 8886 8990 9732 9817 10888 1391
Size: 261 104 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 672 629 532 399
283 279 277 261 216 213 185 176
175 175 158 146 141 138 134 128
122 121 118 104 98 91 87 85
80 66 63 63 61 54 48 44
43 42 41 32 30 29 23 22
19 18 15 14 14 14
BspEI T'CCGG_A
Cuts at: 11334 11334
Size: 11671
BspHI T'CATG_A
Cuts at: 8065 9560 11290 8065
Size: 1495 1730 8446
Fragments arranged by size:
8446 1730 1495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 2245 2790 4145 4285 5311 5586 5824
Size: 1319 82 545 1355 140 1026 275 238
Cuts at: 5824 5973 6181 8147 8408 9026 844
Size: 149 208 1966 261 618 3489
Fragments arranged by size:
3489 1966 1355 1319 1026 618 545 275
261 238 208 149 140 82
BsrBI CCG'CTC
Cuts at: 1394 1823 4819 7495 8860 9558 1394
Size: 429 2996 2676 1365 698 3507
Fragments arranged by size:
3507 2996 2676 1365 698 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5691 10166 10340 65
Size: 1183 4443 4475 174 1396
Fragments arranged by size:
4475 4443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2706 2739 2886 3137 3838 3872 3985
Size: 110 896 33 147 251 701 34 113
Cuts at: 3985 4908 5855 6891 10315 10502 1700
Size: 923 947 1036 3424 187 2869
Fragments arranged by size:
3424 2869 1036 947 923 896 701 251
187 147 113 110 34 33
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11671
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11671
BssSI C'ACGA_G
Cuts at: 4724 6632 7545 9731 10743 4724
Size: 1908 913 2186 1012 5652
Fragments arranged by size:
5652 2186 1908 1012 913
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5578 6988 7243 794
Size: 4784 1410 255 5222
Fragments arranged by size:
5222 4784 1410 255
BstBI TT'CG_AA
Cuts at: 2927 10475 2927
Size: 7548 4123
BstEII G'GTnAC_C
Cuts at: 1184 4573 4688 1184
Size: 3389 115 8167
Fragments arranged by size:
8167 3389 115
BstXI CCAn_nnnn'nTGG
Cuts at: 3788 3899 4081 4685 5410 6175 7204 7373 7391
Size: 111 182 604 725 765 1029 169 18
Cuts at: 7391 7801 8695 3788
Size: 410 894 6764
Fragments arranged by size:
6764 1029 894 765 725 604 410 182
169 111 18
BstYI r'GATC_y
Cuts at: 1972 3996 4308 5079 5310 5742 6526 9001 9368
Size: 2024 312 771 231 432 784 2475 367
Cuts at: 9368 9753 9770 11222 11308 11320 11666 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2475 2024 1977 1452 784 771 432 385
367 346 312 231 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2480 2501 3213 3729 4734 5261 5979 6016 6108
Size: 21 712 516 1005 527 718 37 92
Cuts at: 6108 6818 2480
Size: 710 7333
Fragments arranged by size:
7333 1005 718 712 710 527 516 92
37 21
BtgI C'CryG_G
Cuts at: 513 1065 2018 2088 2133 2790 2901 2957 3228
Size: 552 953 70 45 657 111 56 271
Cuts at: 3228 3436 3810 3895 4080 4269 4828 5302 5634
Size: 208 374 85 185 189 559 474 332
Cuts at: 5634 5646 7176 7366 7437 8165 8604 513
Size: 12 1530 190 71 728 439 3580
Fragments arranged by size:
3580 1530 953 728 657 559 552 474
439 374 332 271 208 190 189 185
111 85 71 70 56 45 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3948 5154 6006 487
Size: 683 56 2722 1206 852 6152
Fragments arranged by size:
6152 2722 1206 852 683 56
BtsI GCAGTG_nn'
Cuts at: 4376 4993 5346 5488 5814 5946 6137 6468 8245
Size: 617 353 142 326 132 191 331 1777
Cuts at: 8245 9187 9981 10001 4376
Size: 942 794 20 6046
Fragments arranged by size:
6046 1777 942 794 617 353 331 326
191 142 132 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11636
DraI TTT'AAA
Cuts at: 8975 8975
Size: 11671
DraIII CAC_nnn'GTG
Cuts at: 2338 4356 5852 2338
Size: 2018 1496 8157
Fragments arranged by size:
8157 2018 1496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3080 6335 8201 8777 10678 809
Size: 507 1764 3255 1866 576 1901 1802
Fragments arranged by size:
3255 1901 1866 1802 1764 576 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2073 2130 2533
Size: 54 1425 50 275 18 243 57 403
Cuts at: 2533 2841 3088 3221 3321 3957 3975 3983 3987
Size: 308 247 133 100 636 18 8 4
Cuts at: 3987 4534 4567 4906 5031 5211 5391 5445 7062
Size: 547 33 339 125 180 180 54 1617
Cuts at: 7062 7206 7725 8223 8278 8631 8875 8956 9118
Size: 144 519 498 55 353 244 81 162
Cuts at: 9118 10007 11636 8
Size: 889 1629 43
Fragments arranged by size:
1629 1617 1425 889 636 547 519 498
403 353 339 308 275 247 244 243
180 180 162 144 133 125 100 81
57 55 54 54 50 43 33 18
18 8 4
EagI C'GGCC_G
Cuts at: 1537 1812 3983 5445 9118 1537
Size: 275 2171 1462 3673 4090
Fragments arranged by size:
4090 3673 2171 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2580 5261 5834 6276 7253 8738 9601 1353
Size: 1227 2681 573 442 977 1485 863 3423
Fragments arranged by size:
3423 2681 1485 1227 977 863 573 442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6668 7813 8243 10258 10632 10778
Size: 1064 138 5079 1145 430 2015 374 146
Cuts at: 10778 387
Size: 1280
Fragments arranged by size:
5079 2015 1280 1145 1064 430 374 146
138
EcoICRI GAG'CTC
Cuts at: 3445 7030 3445
Size: 3585 8086
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2283 2604 2972 2993 3170 3584
Size: 393 506 145 321 368 21 177 414
Cuts at: 3584 3986 4307 4391 4445 4457 5119 5378 5386
Size: 402 321 84 54 12 662 259 8
Cuts at: 5386 5741 5810 6007 6077 6082 6218 6289 6776
Size: 355 69 197 70 5 136 71 487
Cuts at: 6776 6814 7016 7400 7874 7987 8059 8282 8399
Size: 38 202 384 474 113 72 223 117
Cuts at: 8399 8456 8874 9734 10331 11118 11386 1239
Size: 57 418 860 597 787 268 1524
Fragments arranged by size:
1524 860 787 662 597 506 487 474
418 414 402 393 384 368 355 321
321 268 259 223 202 197 177 145
136 117 113 84 72 71 70 69
57 54 38 21 12 8 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2479 3258 3380 4439 4805 5421 6224 7949 8261
Size: 779 122 1059 366 616 803 1725 312
Cuts at: 8261 8892 2479
Size: 631 5258
Fragments arranged by size:
5258 1725 1059 803 779 631 616 366
312 122
EcoO109I rG'GnC_Cy
Cuts at: 2428 2460 2628 3725 3769 3784 4119 4120 5188
Size: 32 168 1097 44 15 335 1 1068
Cuts at: 5188 5257 5575 5704 5956 6156 6249 6877 6878
Size: 69 318 129 252 200 93 628 1
Cuts at: 6878 6928 7147 7431 7670 7955 8267 8353 8403
Size: 50 219 284 239 285 312 86 50
Cuts at: 8403 8404 8600 8779 8882 8883 2428
Size: 1 196 179 103 1 5216
Fragments arranged by size:
5216 1097 1068 628 335 318 312 285
284 252 239 219 200 196 179 168
129 103 93 86 69 50 50 44
32 15 1 1 1 1
EcoRI G'AATT_C
Cuts at: 8980 9358 8980
Size: 378 11293
EcoRV GAT'ATC
Cuts at: 4954 4954
Size: 11671
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2210 3535 4520 8659 2210
Size: 1325 985 4139 5222
Fragments arranged by size:
5222 4139 1325 985
FseI GG_CCGG'CC
Cuts at: 3989 3989
Size: 11671
FspI TGC'GCA
Cuts at: 3395 6599 10177 3395
Size: 3204 3578 4889
Fragments arranged by size:
4889 3578 3204
HaeII r_GCGC'y
Cuts at: 1678 3820 5886 6261 6584 7114 7231 7879 8717
Size: 2142 2066 375 323 530 117 648 838
Cuts at: 8717 10818 1678
Size: 2101 2531
Fragments arranged by size:
2531 2142 2101 2066 838 648 530 375
323 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3740 3772 5019 5051 5132
Size: 32 135 32 2168 32 1247 32 81
Cuts at: 5132 5164 5520 5552 5984 5999 6016 6031 6497
Size: 32 356 32 432 15 17 15 466
Cuts at: 6497 6503 6529 6535 7574 7606 10312 10344 10386
Size: 6 26 6 1039 32 2706 32 42
Cuts at: 10386 10418 1373
Size: 32 2626
Fragments arranged by size:
2706 2626 2168 1247 1039 466 432 356
135 81 42 32 32 32 32 32
32 32 32 32 26 17 15 15
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3643 9015 9261 669
Size: 988 281 1705 5372 246 3079
Fragments arranged by size:
5372 3079 1705 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3111 3192 7866 748
Size: 2363 81 4674 4553
Fragments arranged by size:
4674 4553 2363 81
HpaI GTT'AAC
Cuts at: 9261 9261
Size: 11671
KasI G'GCGC_C
Cuts at: 5882 6580 5882
Size: 698 10973
KpnI G_GTAC'C
Cuts at: 2814 4234 8996 2814
Size: 1420 4762 5489
Fragments arranged by size:
5489 4762 1420
MfeI C'AATT_G
Cuts at: 9270 9270
Size: 11671
MluI A'CGCG_T
Cuts at: 11547 11547
Size: 11671
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3573 3846 5352 10785 10969 1305
Size: 2268 273 1506 5433 184 2007
Fragments arranged by size:
5433 2268 2007 1506 273 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2075 2132 3090 3223 3977
Size: 54 1425 343 243 57 958 133 754
Cuts at: 3977 4536 5033 5393 7208 7727 8225 8633 8877
Size: 559 497 360 1815 519 498 408 244
Cuts at: 8877 8958 10
Size: 81 2723
Fragments arranged by size:
2723 1815 1425 958 754 559 519 498
497 408 360 343 244 243 133 81
57 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2339 2759 2829 2877 3897 4683 4871
Size: 648 1173 420 70 48 1020 786 188
Cuts at: 4871 5447 5670 6593 7371 7404 7475 7832 8687
Size: 576 223 923 778 33 71 357 855
Cuts at: 8687 8867 9629 9988 10147 518
Size: 180 762 359 159 2042
Fragments arranged by size:
2042 1173 1020 923 855 786 778 762
648 576 420 359 357 223 188 180
159 71 70 48 33
MspA1I CmG'CkG
Cuts at: 1606 2020 2090 2161 2216 3295 3556 3721 3897
Size: 414 70 71 55 1079 261 165 176
Cuts at: 3897 4204 5170 5317 5513 5768 5788 6071 6289
Size: 307 966 147 196 255 20 283 218
Cuts at: 6289 6619 7036 7162 7282 7339 7570 7624 7759
Size: 330 417 126 120 57 231 54 135
Cuts at: 7759 7840 8329 8627 8867 9087 9764 10912 11157
Size: 81 489 298 240 220 677 1148 245
Cuts at: 11157 1606
Size: 2120
Fragments arranged by size:
2120 1148 1079 966 677 489 417 414
330 307 298 283 261 255 245 240
231 220 218 196 176 165 147 135
126 120 81 71 70 57 55 54
20
NaeI GCC'GGC
Cuts at: 2708 2741 3139 3840 3874 3987 4910 5857 6893
Size: 33 398 701 34 113 923 947 1036
Cuts at: 6893 2708
Size: 7486
Fragments arranged by size:
7486 1036 947 923 701 398 113 34
33
NarI GG'CG_CC
Cuts at: 5883 6581 5883
Size: 698 10973
NcoI C'CATG_G
Cuts at: 513 1065 3228 3436 4828 5634 8165 8604 513
Size: 552 2163 208 1392 806 2531 439 3580
Fragments arranged by size:
3580 2531 2163 1392 806 552 439 208
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11671
NgoMIV G'CCGG_C
Cuts at: 2706 2739 3137 3838 3872 3985 4908 5855 6891
Size: 33 398 701 34 113 923 947 1036
Cuts at: 6891 2706
Size: 7486
Fragments arranged by size:
7486 1036 947 923 701 398 113 34
33
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11671
NotI GC'GGCC_GC
Cuts at: 9118 9118
Size: 11671
NsiI A_TGCA'T
Cuts at: 8110 8110
Size: 11671
NspI r_CATG'y
Cuts at: 1132 1229 3359 7768 8150 8671 9029 10574 1132
Size: 97 2130 4409 382 521 358 1545 2229
Fragments arranged by size:
4409 2229 2130 1545 521 382 358 97
PasI CC'CwG_GG
Cuts at: 1348 2614 6785 8173 1348
Size: 1266 4171 1388 4846
Fragments arranged by size:
4846 4171 1388 1266
PciI A'CATG_T
Cuts at: 8667 10570 8667
Size: 1903 9768
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 2638 4356 6382 7285 7870 8332 1108
Size: 1230 300 1718 2026 903 585 462 4447
Fragments arranged by size:
4447 2026 1718 1230 903 585 462 300
PfoI T'CCnGG_A
Cuts at: 3009 3492 3699 6266 8259 8890 3009
Size: 483 207 2567 1993 631 5790
Fragments arranged by size:
5790 2567 1993 631 483 207
PmeI GTTT'AAAC
Cuts at: 8975 8975
Size: 11671
PmlI CAC'GTG
Cuts at: 4595 4595
Size: 11671
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2384 2416 5367 5399 9740 9772 11253 11285 2384
Size: 32 2951 32 4341 32 1481 32 2770
Fragments arranged by size:
4341 2951 2770 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 2460 3784 5188 5257 5575 5956 6249 7147
Size: 32 1324 1404 69 318 381 293 898
Cuts at: 7147 7955 8267 8353 8779 2428
Size: 808 312 86 426 5320
Fragments arranged by size:
5320 1404 1324 898 808 426 381 318
312 293 86 69 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11671
PsiI TTA'TAA
Cuts at: 9241 9241
Size: 11671
PspOMI G'GGCC_C
Cuts at: 2627 4119 5704 6155 6877 7563 7670 8403 8882
Size: 1492 1585 451 722 686 107 733 479
Cuts at: 8882 2627
Size: 5416
Fragments arranged by size:
5416 1585 1492 733 722 686 479 451
107
PspXI vC'TCGA_Gb
Cuts at: 3446 6035 3446
Size: 2589 9082
PstI C_TGCA'G
Cuts at: 830 2160 2166 2235 2256 2295 3146 3945 4128
Size: 1330 6 69 21 39 851 799 183
Cuts at: 4128 4143 5322 5821 6192 6225 6453 7332 7348
Size: 15 1179 499 371 33 228 879 16
Cuts at: 7348 8922 9023 830
Size: 1574 101 3478
Fragments arranged by size:
3478 1574 1330 1179 879 851 799 499
371 228 183 101 69 39 33 21
16 15 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11671
PvuII CAG'CTG
Cuts at: 2161 2216 3295 3556 5170 5317 5513 5768 5788
Size: 55 1079 261 1614 147 196 255 20
Cuts at: 5788 6289 6619 7036 7282 7339 7570 7759 7840
Size: 501 330 417 246 57 231 189 81
Cuts at: 7840 8329 8867 2161
Size: 489 538 4965
Fragments arranged by size:
4965 1614 1079 538 501 489 417 330
261 255 246 231 196 189 147 81
57 55 20
SacI G_AGCT'C
Cuts at: 3447 7032 3447
Size: 3585 8086
SacII CC_GC'GG
Cuts at: 2021 2091 3898 2021
Size: 70 1807 9794
Fragments arranged by size:
9794 1807 70
SalI G'TCGA_C
Cuts at: 1936 9013 1936
Size: 7077 4594
SanDI GG'GwC_CC
Cuts at: 2460 7147 7955 8267 2460
Size: 4687 808 312 5864
Fragments arranged by size:
5864 4687 808 312
SapI GCTCTTCn'nnn_
Cuts at: 5834 6276 7253 8738 5834
Size: 442 977 1485 8767
Fragments arranged by size:
8767 1485 977 442
SbfI CC_TGCA'GG
Cuts at: 2256 6192 9023 2256
Size: 3936 2831 4904
Fragments arranged by size:
4904 3936 2831
ScaI AGT'ACT
Cuts at: 5277 8963 9919 11631 5277
Size: 3686 956 1712 5317
Fragments arranged by size:
5317 3686 1712 956
SexAI A'CCwGG_T
Cuts at: 5862 5862
Size: 11671
SfcI C'TryA_G
Cuts at: 826 1045 2156 2162 2231 2252 2291 3142 3941
Size: 219 1111 6 69 21 39 851 799
Cuts at: 3941 4124 4139 4511 5118 5318 5599 5817 6188
Size: 183 15 372 607 200 281 218 371
Cuts at: 6188 6221 6449 7328 7344 8515 8918 9019 10154
Size: 33 228 879 16 1171 403 101 1135
Cuts at: 10154 10835 11026 826
Size: 681 191 1471
Fragments arranged by size:
1471 1171 1135 1111 879 851 799 681
607 403 372 371 281 228 219 218
200 191 183 101 69 39 33 21
16 15 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 7724 7724
Size: 11671
SfoI GGC'GCC
Cuts at: 5884 6582 5884
Size: 698 10973
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11671
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11671
SmaI CCC'GGG
Cuts at: 1872 3462 5096 7055 7562 8317 8998 1872
Size: 1590 1634 1959 507 755 681 4545
Fragments arranged by size:
4545 1959 1634 1590 755 681 507
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3344 3446 5418 6035 6240 8357
Size: 197 926 1401 102 1972 617 205 2117
Cuts at: 8357 8657 8948 9775 10676 10938 820
Size: 300 291 827 901 262 1553
Fragments arranged by size:
2117 1972 1553 1401 926 901 827 617
300 291 262 205 197 102
SnaBI TAC'GTA
Cuts at: 493 9462 493
Size: 8969 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11671
SphI G_CATG'C
Cuts at: 1132 9029 1132
Size: 7897 3774
SspI AAT'ATT
Cuts at: 5 52 6970 9595 5
Size: 47 6918 2625 2081
Fragments arranged by size:
6918 2625 2081 47
StuI AGG'CCT
Cuts at: 1517 2996 3743 5369 5984 6021 6447 7463 7514
Size: 1479 747 1626 615 37 426 1016 51
Cuts at: 7514 1517
Size: 5674
Fragments arranged by size:
5674 1626 1479 1016 747 615 426 51
37
StyI C'CwwG_G
Cuts at: 513 1065 2049 2464 2718 3228 3436 3787 4027
Size: 552 984 415 254 510 208 351 240
Cuts at: 4027 4828 5634 5886 8165 8604 8634 9110 513
Size: 801 806 252 2279 439 30 476 3074
Fragments arranged by size:
3074 2279 984 806 801 552 510 476
439 415 351 254 252 240 208 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2159 3305 3860 4409 4562 5264 5917
Size: 1255 579 1146 555 549 153 702 653
Cuts at: 5917 6014 7397 7646 8950 8956 9698 9715 9868
Size: 97 1383 249 1304 6 742 17 153
Cuts at: 9868 10053 10498 325
Size: 185 445 1498
Fragments arranged by size:
1498 1383 1304 1255 1146 742 702 653
579 555 549 445 249 185 153 153
97 17 6
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5275 8961 9917
Size: 274 80 33 51 1443 3298 3686 956
Cuts at: 9917 11629 96
Size: 1712 138
Fragments arranged by size:
3686 3298 1712 1443 956 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4179 4211 8439 8471 11631 11663 4179
Size: 32 4228 32 3160 32 4187
Fragments arranged by size:
4228 4187 3160 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11671
XbaI T'CTAG_A
Cuts at: 9007 9007
Size: 11671
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 3967 4063 4420 5353 5510 7075 7174 8188
Size: 1362 96 357 933 157 1565 99 1014
Cuts at: 8188 2605
Size: 6088
Fragments arranged by size:
6088 1565 1362 1014 933 357 157 99
96
XhoI C'TCGA_G
Cuts at: 1943 3446 6035 1943
Size: 1503 2589 7579
Fragments arranged by size:
7579 2589 1503
XmaI C'CCGG_G
Cuts at: 1870 3460 5094 7053 7560 8315 8996 1870
Size: 1590 1634 1959 507 755 681 4545
Fragments arranged by size:
4545 1959 1634 1590 755 681 507
XmnI GAAnn'nnTTC
Cuts at: 9800 9800
Size: 11671
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8773 9479 276
Size: 53 83 186 936 7239 706 2468
Fragments arranged by size:
7239 2468 936 706 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BsiWI BstZ17I FspAI NruI PacI
PsrI RsrII SrfI SwaI