Restriction map for
pFN21AB9558
(Circular) (Six-base) MAPSORT of: pFN21AB9558.seq Check: 498 from: 1 to: 6754
pFN21AB9558 6754 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4097 1937
Size: 2160 4594
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2119 2628 3316 4817 6201 6469 1632
Size: 487 509 688 1501 1384 268 1917
Fragments arranged by size:
1917 1501 1384 688 509 487 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 5653 6630 1203
Size: 4450 977 1327
Fragments arranged by size:
4450 1327 977
AgeI A'CCGG_T
Cuts at: 3021 5585 3021
Size: 2564 4190
AhdI GACnn_n'nnGTC
Cuts at: 2390 5483 2390
Size: 3093 3661
AleI CACnn'nnGTG
Cuts at: 1166 2810 3375 4050 1166
Size: 1644 565 675 3870
Fragments arranged by size:
3870 1644 675 565
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6754
AlwNI CAG_nnn'CTG
Cuts at: 2086 2272 2314 2524 2959 4497 6069 2086
Size: 186 42 210 435 1538 1572 2771
Fragments arranged by size:
2771 1572 1538 435 210 186 42
ApaI G_GGCC'C
Cuts at: 2018 2643 2725 2018
Size: 625 82 6047
Fragments arranged by size:
6047 625 82
ApaLI G'TGCA_C
Cuts at: 4811 5967 4811
Size: 1156 5598
ApoI r'AATT_y
Cuts at: 1493 2900 3160 3534 4063 4289 4441 6550 1493
Size: 1407 260 374 529 226 152 2109 1697
Fragments arranged by size:
2109 1697 1407 529 374 260 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2114 2169 2976 3566 4079 1870
Size: 73 74 97 55 807 590 513 4545
Fragments arranged by size:
4545 807 590 513 97 74 73 55
BamHI G'GATC_C
Cuts at: 2707 2836 4084 4451 2707
Size: 129 1248 367 5010
Fragments arranged by size:
5010 1248 367 129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2804 2986 3040 3189 3441
Size: 325 217 627 1017 182 54 149 252
Cuts at: 3441 3525 3864 4075 5530 618
Size: 84 339 211 1455 1842
Fragments arranged by size:
1842 1455 1017 627 339 325 252 217
211 182 149 84 54
BanII G_rGCy'C
Cuts at: 1391 2018 2024 2043 2114 2643 2725 2873 3706
Size: 627 6 19 71 529 82 148 833
Cuts at: 3706 4073 1391
Size: 367 4072
Fragments arranged by size:
4072 833 627 529 367 148 82 71
19 6
BbeI G_GCGC'C
Cuts at: 3044 3044
Size: 6754
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2628 3016 928
Size: 971 729 388 4666
Fragments arranged by size:
4666 971 729 388
BbvCI CC'TCA_GC
Cuts at: 2180 2180
Size: 6754
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3100 3134 4945 4979 1903
Size: 34 1163 34 1811 34 3678
Fragments arranged by size:
3678 1811 1163 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4646 5856 4646
Size: 1210 5544
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2877 3710 5365
Size: 107 122 71 631 605 1205 833 1655
Cuts at: 5365 136
Size: 1525
Fragments arranged by size:
1655 1525 1205 833 631 605 122 107
71
BglII A'GATC_T
Cuts at: 2241 3495 6749 2241
Size: 1254 3254 2246
Fragments arranged by size:
3254 2246 1254
BlpI GC'TnA_GC
Cuts at: 4171 4171
Size: 6754
Bme1580I G_kGCm'C
Cuts at: 1790 2018 2643 2725 2991 3482 4815 5971 1790
Size: 228 625 82 266 491 1333 1156 2573
Fragments arranged by size:
2573 1333 1156 625 491 266 228 82
BmgBI CAC'GTC
Cuts at: 1318 2668 1318
Size: 1350 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2638 2646 2781
Size: 416 53 83 399 24 1221 8 135
Cuts at: 2781 2835 3110 3468 4485 5443 442
Size: 54 275 358 1017 958 1753
Fragments arranged by size:
1753 1221 1017 958 416 399 358 275
135 83 54 53 24 8
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2095 2498 2713 2792 2871 3013 3564
Size: 839 17 403 215 79 79 142 551
Cuts at: 3564 5414 1239
Size: 1850 2579
Fragments arranged by size:
2579 1850 839 551 403 215 142 79
79 17
Bpu10I CC'TnA_GC
Cuts at: 2180 2867 3000 2180
Size: 687 133 5934
Fragments arranged by size:
5934 687 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3070 4879 5744 6042 3070
Size: 1809 865 298 3782
Fragments arranged by size:
3782 1809 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3967 5417 882
Size: 617 2468 1450 2219
Fragments arranged by size:
2468 2219 1450 617
BsaAI yAC'GTr
Cuts at: 493 1204 4545 493
Size: 711 3341 2702
Fragments arranged by size:
3341 2702 711
BsaBI GATnn'nnATC
Cuts at: 6748 6748
Size: 6754
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3041 4561 4943
Size: 53 83 186 936 476 1032 1520 382
Cuts at: 4943 5578 275
Size: 635 1451
Fragments arranged by size:
1520 1451 1032 936 635 476 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2320 3021 5187 5585 5859 6006 6417 2320
Size: 701 2166 398 274 147 411 2657
Fragments arranged by size:
2657 2166 701 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4006 4036 1178
Size: 30 165 30 2603 30 3896
Fragments arranged by size:
3896 2603 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2444 2705 3311 1186
Size: 889 369 261 606 4629
Fragments arranged by size:
4629 889 606 369 261
BseYI C'CCAG_C
Cuts at: 2534 2881 3004 5957 6453 2534
Size: 347 123 2953 496 2835
Fragments arranged by size:
2953 2835 496 347 123
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2327 2762 3220 1163
Size: 573 591 435 458 4697
Fragments arranged by size:
4697 591 573 458 435
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3026 3110 4204 4965 5993 1540
Size: 275 186 1025 84 1094 761 1028 2301
Fragments arranged by size:
2301 1094 1028 1025 761 275 186 84
BsiHKAI G_wGCw'C
Cuts at: 3286 4815 4900 5971 3286
Size: 1529 85 1071 4069
Fragments arranged by size:
4069 1529 1071 85
BsmI GAATG_Cn'
Cuts at: 4265 4358 4265
Size: 93 6661
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2024 2043 2114 2643 2725 2873
Size: 399 228 6 19 71 529 82 148
Cuts at: 2873 2991 3286 3482 3706 4073 4815 4900 5971
Size: 118 295 196 224 367 742 85 1071
Cuts at: 5971 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 529 399 367 295 228
224 196 148 118 85 82 71 19
6
BspEI T'CCGG_A
Cuts at: 2320 6417 2320
Size: 4097 2657
BspHI T'CATG_A
Cuts at: 3016 3622 3904 4643 6373 3016
Size: 606 282 739 1730 3397
Fragments arranged by size:
3397 1730 739 606 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4109 844
Size: 3265 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2328 4641 1394
Size: 429 505 2313 3507
Fragments arranged by size:
3507 2313 505 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2679 5249 5423 65
Size: 1183 1431 2570 174 1396
Fragments arranged by size:
2570 1431 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 3021 5398 5585 1700
Size: 110 374 837 2377 187 2869
Fragments arranged by size:
2869 2377 837 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 2676 96
Size: 2580 4174
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 2454 4814 5826 2454
Size: 2360 1012 3382
Fragments arranged by size:
3382 2360 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2314 2524 2579 3402 3766 794
Size: 1520 210 55 823 364 3782
Fragments arranged by size:
3782 1520 823 364 210 55
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 2341 1184
Size: 1157 5597
BstYI r'GATC_y
Cuts at: 1972 2241 2292 2707 2836 3495 3848 4084 4451
Size: 269 51 415 129 659 353 236 367
Cuts at: 4451 4836 4853 6305 6391 6403 6749 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 659 415 385 367 353 346
269 236 129 86 51 17 12
Bsu36I CC'TnA_GG
Cuts at: 2952 2952
Size: 6754
BtgI C'CryG_G
Cuts at: 513 1065 2004 2203 2401 3697 513
Size: 552 939 199 198 1296 3570
Fragments arranged by size:
3570 1296 939 552 199 198
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6015
Fragments arranged by size:
6015 683 56
BtsI GCAGTG_nn'
Cuts at: 4270 5064 5084 4270
Size: 794 20 5940
Fragments arranged by size:
5940 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6719
DraI TTT'AAA
Cuts at: 4058 4058
Size: 6754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5761 809
Size: 507 4445 1802
Fragments arranged by size:
4445 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4201 5090 6719
Size: 54 1425 50 275 18 2371 889 1629
Cuts at: 6719 8
Size: 43
Fragments arranged by size:
2371 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2889 4684 1353
Size: 1536 1795 3423
Fragments arranged by size:
3423 1795 1536
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2095 2119 2498 2628 2713 2792
Size: 393 446 17 24 379 130 85 79
Cuts at: 2792 2871 3013 3316 3564 4817 5414 6201 6469
Size: 79 142 303 248 1253 597 787 268
Cuts at: 6469 1239
Size: 1524
Fragments arranged by size:
1524 1253 787 597 446 393 379 303
268 248 142 130 85 79 79 24
17
EcoNI CCTnn'n_nnAGG
Cuts at: 2100 2285 2351 2607 3293 2100
Size: 185 66 256 686 5561
Fragments arranged by size:
5561 686 256 185 66
EcoO109I rG'GnC_Cy
Cuts at: 2531 2548 2640 2721 2722 2797 2863 2531
Size: 17 92 81 1 75 66 6422
Fragments arranged by size:
6422 92 81 75 66 17 1
EcoRI G'AATT_C
Cuts at: 3534 4063 4441 3534
Size: 529 378 5847
Fragments arranged by size:
5847 529 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3742 3742
Size: 6754
FspI TGC'GCA
Cuts at: 2228 5260 2228
Size: 3032 3722
FspAI rTGC'GCAy
Cuts at: 2228 2228
Size: 6754
HaeII r_GCGC'y
Cuts at: 1678 3044 5901 1678
Size: 1366 2857 2531
Fragments arranged by size:
2857 2531 1366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2376 2408 3487 3519 3908
Size: 32 135 32 804 32 1079 32 389
Cuts at: 3908 3940 4006 4038 5395 5427 5469 5501 1373
Size: 32 66 32 1357 32 42 32 2626
Fragments arranged by size:
2626 1357 1079 804 389 135 66 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4098 4344 669
Size: 988 281 2160 246 3079
Fragments arranged by size:
3079 2160 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3751 4003 748
Size: 3003 252 3499
Fragments arranged by size:
3499 3003 252
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KasI G'GCGC_C
Cuts at: 3040 3040
Size: 6754
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 4353 4353
Size: 6754
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3453 3537 5868 6052 1305
Size: 2148 84 2331 184 2007
Fragments arranged by size:
2331 2148 2007 184 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4932
Fragments arranged by size:
4932 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2810 3375 4050 4712 5071 5230 518
Size: 648 1644 565 675 662 359 159 2042
Fragments arranged by size:
2042 1644 675 662 648 565 359 159
MspA1I CmG'CkG
Cuts at: 1606 2162 3209 4170 4847 5995 6240 1606
Size: 556 1047 961 677 1148 245 2120
Fragments arranged by size:
2120 1148 1047 961 677 556 245
NaeI GCC'GGC
Cuts at: 2186 2186
Size: 6754
NarI GG'CG_CC
Cuts at: 3041 3041
Size: 6754
NcoI C'CATG_G
Cuts at: 513 1065 2004 2203 2401 3697 513
Size: 552 939 199 198 1296 3570
Fragments arranged by size:
3570 1296 939 552 199 198
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6754
NgoMIV G'CCGG_C
Cuts at: 2184 2184
Size: 6754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 3206 3769 4112 5657 1132
Size: 97 1977 563 343 1545 2229
Fragments arranged by size:
2229 1977 1545 563 343 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6754
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3056 1108
Size: 1948 4806
PfoI T'CCnGG_A
Cuts at: 2887 2887
Size: 6754
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4823 4855 6336 6368 4823
Size: 32 1481 32 5209
Fragments arranged by size:
5209 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2797 2548
Size: 249 6505
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 3558 4324 3558
Size: 766 5988
PspOMI G'GGCC_C
Cuts at: 2014 2639 2721 2014
Size: 625 82 6047
Fragments arranged by size:
6047 625 82
PstI C_TGCA'G
Cuts at: 830 2520 3123 4106 830
Size: 1690 603 983 3478
Fragments arranged by size:
3478 1690 983 603
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6754
PvuII CAG'CTG
Cuts at: 2162 3209 2162
Size: 1047 5707
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SanDI GG'GwC_CC
Cuts at: 2548 2548
Size: 6754
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SfcI C'TryA_G
Cuts at: 826 1045 2516 3119 4102 5237 5918 6109 826
Size: 219 1471 603 983 1135 681 191 1471
Fragments arranged by size:
1471 1471 1135 983 681 603 219 191
SfoI GGC'GCC
Cuts at: 3042 3042
Size: 6754
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 2019 2171 4081 1872
Size: 147 152 1910 4545
Fragments arranged by size:
4545 1910 152 147
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3085 4858 5759 6021 820
Size: 197 926 1142 1773 901 262 1553
Fragments arranged by size:
1773 1553 1142 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 3769 4112 1132
Size: 2637 343 3774
Fragments arranged by size:
3774 2637 343
SrfI GCCC'GGGC
Cuts at: 2019 2019
Size: 6754
SspI AAT'ATT
Cuts at: 5 52 4678 5
Size: 47 4626 2081
Fragments arranged by size:
4626 2081 47
StuI AGG'CCT
Cuts at: 1517 2120 3071 1517
Size: 603 951 5200
Fragments arranged by size:
5200 951 603
StyI C'CwwG_G
Cuts at: 513 1065 2004 2203 2401 3072 3697 4193 513
Size: 552 939 199 198 671 625 496 3074
Fragments arranged by size:
3074 939 671 625 552 496 199 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2122 2196 2822 3124 3215 3359 3383
Size: 1255 542 74 626 302 91 144 24
Cuts at: 3383 4781 4798 4951 5136 5581 325
Size: 1398 17 153 185 445 1498
Fragments arranged by size:
1498 1398 1255 626 542 445 302 185
153 144 91 74 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2676 5000 6712
Size: 274 80 33 51 1443 699 2324 1712
Cuts at: 6712 96
Size: 138
Fragments arranged by size:
2324 1712 1443 699 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 6714 6746 2403
Size: 32 4279 32 2411
Fragments arranged by size:
4279 2411 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6754
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2159 2159
Size: 6754
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6754
XmaI C'CCGG_G
Cuts at: 1870 2017 2169 4079 1870
Size: 147 152 1910 4545
Fragments arranged by size:
4545 1910 152 147
XmnI GAAnn'nnTTC
Cuts at: 2919 3329 3581 3752 4883 2919
Size: 410 252 171 1131 4790
Fragments arranged by size:
4790 1131 410 252 171
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SwaI