Restriction map for
pFN21AB9553
(Circular) (Six-base) MAPSORT of: pFN21AB9553.seq Check: 5785 from: 1 to: 5488
pFN21AB9553 5488 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2518 2518
Size: 5488
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2758 3298 278
Size: 53 83 186 936 1222 540 2468
Fragments arranged by size:
2468 1222 936 540 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2702 2831 1937
Size: 765 129 4594
Fragments arranged by size:
4594 765 129
Acc65I G'GTAC_C
Cuts at: 2809 2809
Size: 5488
AclI AA'CG_TT
Cuts at: 1563 2787 3615 3988 1563
Size: 1224 828 373 3063
Fragments arranged by size:
3063 1224 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2457 2576 3551 4935 5203 1632
Size: 825 119 975 1384 268 1917
Fragments arranged by size:
1917 1384 975 825 268 119
AfeI AGC'GCT
Cuts at: 2411 2411
Size: 5488
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5291
AflIII A'CryG_T
Cuts at: 1203 4387 5364 1203
Size: 3184 977 1327
Fragments arranged by size:
3184 1327 977
AgeI A'CCGG_T
Cuts at: 4319 4319
Size: 5488
AhdI GACnn_n'nnGTC
Cuts at: 4217 4217
Size: 5488
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5488
AlwNI CAG_nnn'CTG
Cuts at: 2110 2605 2674 3231 4803 2110
Size: 495 69 557 1572 2795
Fragments arranged by size:
2795 1572 557 495 69
ApaLI G'TGCA_C
Cuts at: 2514 3545 4701 2514
Size: 1031 1156 3301
Fragments arranged by size:
3301 1156 1031
ApoI r'AATT_y
Cuts at: 1493 2797 3023 3175 5284 1493
Size: 1304 226 152 2109 1697
Fragments arranged by size:
2109 1697 1304 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2780 4042 160
Size: 872 1748 1262 1606
Fragments arranged by size:
1748 1606 1262 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
AvaI C'yCGr_G
Cuts at: 1870 1943 2210 2813 1870
Size: 73 267 603 4545
Fragments arranged by size:
4545 603 267 73
BamHI G'GATC_C
Cuts at: 2818 3185 2818
Size: 367 5121
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2122 2809 4264 618
Size: 325 217 627 335 687 1455 1842
Fragments arranged by size:
1842 1455 687 627 335 325 217
BanII G_rGCy'C
Cuts at: 1391 2095 2210 2693 2807 1391
Size: 704 115 483 114 4072
Fragments arranged by size:
4072 704 483 115 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4517
BbvCI CC'TCA_GC
Cuts at: 2225 2225
Size: 5488
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3679 3713 1903
Size: 34 1742 34 3678
Fragments arranged by size:
3678 1742 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3380 4590 3380
Size: 1210 4278
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5488
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 4099 136
Size: 107 122 71 631 605 433 1994 1525
Fragments arranged by size:
1994 1525 631 605 433 122 107 71
BglII A'GATC_T
Cuts at: 5483 5483
Size: 5488
BlpI GC'TnA_GC
Cuts at: 2905 2905
Size: 5488
Bme1580I G_kGCm'C
Cuts at: 1790 2518 3549 4705 1790
Size: 728 1031 1156 2573
Fragments arranged by size:
2573 1156 1031 728
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5488
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2533 2635 2711
Size: 416 53 83 399 24 1116 102 76
Cuts at: 2711 3219 4177 442
Size: 508 958 1753
Fragments arranged by size:
1753 1116 958 508 416 399 102 83
76 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 2704 4148 1239
Size: 1298 167 1444 2579
Fragments arranged by size:
2579 1444 1298 167
Bpu10I CC'TnA_GC
Cuts at: 2204 2225 2204
Size: 21 5467
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3613 4478 4776 3613
Size: 865 298 4325
Fragments arranged by size:
4325 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4151 882
Size: 617 2652 2219
Fragments arranged by size:
2652 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2513 3279 493
Size: 711 1309 766 2702
Fragments arranged by size:
2702 1309 766 711
BsaBI GATnn'nnATC
Cuts at: 5482 5482
Size: 5488
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2755 3295 3677 4312
Size: 53 83 186 936 1222 540 382 635
Cuts at: 4312 275
Size: 1451
Fragments arranged by size:
1451 1222 936 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3921 4319 4593 4740 5151 3921
Size: 398 274 147 411 4258
Fragments arranged by size:
4258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5263
Fragments arranged by size:
5263 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 2242 2456 2701 1186
Size: 1042 14 214 245 3973
Fragments arranged by size:
3973 1042 245 214 14
BseYI C'CCAG_C
Cuts at: 4691 5187 4691
Size: 496 4992
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2552 1163
Size: 573 816 4099
Fragments arranged by size:
4099 816 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2037 2100 2938 3699 4727 1540
Size: 275 186 36 63 838 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 275 186 63 36
BsiHKAI G_wGCw'C
Cuts at: 2518 2693 3549 3634 4705 2518
Size: 175 856 85 1071 3301
Fragments arranged by size:
3301 1071 856 175 85
BsmI GAATG_Cn'
Cuts at: 2621 2999 3092 2621
Size: 378 93 5017
Fragments arranged by size:
5017 378 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2095 2210 2518 2693 2807 3549 3634
Size: 399 305 115 308 175 114 742 85
Cuts at: 3634 4705 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 308 305 175 115
114 85
BspEI T'CCGG_A
Cuts at: 5151 5151
Size: 5488
BspHI T'CATG_A
Cuts at: 3377 5107 3377
Size: 1730 3758
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2518 2843 844
Size: 1674 325 3489
Fragments arranged by size:
3489 1674 325
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 2160 3375 1394
Size: 429 293 44 1215 3507
Fragments arranged by size:
3507 1215 429 293 44
BsrDI GCAATG_nn'
Cuts at: 65 1248 3983 4157 65
Size: 1183 2735 174 1396
Fragments arranged by size:
2735 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2099 4132 4319 1700
Size: 110 289 2033 187 2869
Fragments arranged by size:
2869 2033 289 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5488
BssHII G'CGCG_C
Cuts at: 1925 2177 1925
Size: 252 5236
BssSI C'ACGA_G
Cuts at: 3548 4560 3548
Size: 1012 4476
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2530 794
Size: 1736 3752
BstBI TT'CG_AA
Cuts at: 4292 4292
Size: 5488
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5488
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5488
BstYI r'GATC_y
Cuts at: 1972 2818 3185 3570 3587 5039 5125 5137 5483
Size: 846 367 385 17 1452 86 12 346
Cuts at: 5483 1972
Size: 1977
Fragments arranged by size:
1977 1452 846 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2567 513
Size: 552 939 563 3434
Fragments arranged by size:
3434 939 563 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4749
Fragments arranged by size:
4749 683 56
BtsI GCAGTG_nn'
Cuts at: 3004 3798 3818 3004
Size: 794 20 4674
Fragments arranged by size:
4674 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5453
DraI TTT'AAA
Cuts at: 2792 2792
Size: 5488
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4495 809
Size: 507 3179 1802
Fragments arranged by size:
3179 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2097 2101
Size: 54 1425 50 275 18 204 63 4
Cuts at: 2101 2478 2935 3824 5453 8
Size: 377 457 889 1629 43
Fragments arranged by size:
1629 1425 889 457 377 275 204 63
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2034 2097 2935 1537
Size: 275 222 63 838 4090
Fragments arranged by size:
4090 838 275 222 63
EarI CTCTTCn'nnn_
Cuts at: 1353 2319 2433 2595 3418 1353
Size: 966 114 162 823 3423
Fragments arranged by size:
3423 966 823 162 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2071 2462 4075 4449 4595 387
Size: 1064 138 482 391 1613 374 146 1280
Fragments arranged by size:
1613 1280 1064 482 391 374 146 138
EcoICRI GAG'CTC
Cuts at: 2691 2691
Size: 5488
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2457 2537 2576 2704 3551 4148 4935
Size: 393 825 80 39 128 847 597 787
Cuts at: 4935 5203 1239
Size: 268 1524
Fragments arranged by size:
1524 847 825 787 597 393 268 128
80 39
EcoO109I rG'GnC_Cy
Cuts at: 2043 2107 2713 2043
Size: 64 606 4818
Fragments arranged by size:
4818 606 64
EcoRI G'AATT_C
Cuts at: 2797 3175 2797
Size: 378 5110
EcoRV GAT'ATC
Cuts at: 2610 2610
Size: 5488
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2075 2075
Size: 5488
FseI GG_CCGG'CC
Cuts at: 2103 2103
Size: 5488
FspI TGC'GCA
Cuts at: 3994 3994
Size: 5488
HaeII r_GCGC'y
Cuts at: 1678 2194 2225 2413 4635 1678
Size: 516 31 188 2222 2531
Fragments arranged by size:
2531 2222 516 188 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2178 2210 2587 2619 4129
Size: 32 135 32 606 32 377 32 1510
Cuts at: 4129 4161 4203 4235 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1510 606 377 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3078 669
Size: 988 281 894 246 3079
Fragments arranged by size:
3079 988 894 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5488
HpaI GTT'AAC
Cuts at: 3078 3078
Size: 5488
KpnI G_GTAC'C
Cuts at: 2813 2813
Size: 5488
MfeI C'AATT_G
Cuts at: 3087 3087
Size: 5488
MluI A'CGCG_T
Cuts at: 5364 5364
Size: 5488
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4602 4786 1305
Size: 3297 184 2007
Fragments arranged by size:
3297 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3666
Fragments arranged by size:
3666 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3446 3805 3964 518
Size: 648 843 1437 359 159 2042
Fragments arranged by size:
2042 1437 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2904 3581 4729 4974 1606
Size: 1298 677 1148 245 2120
Fragments arranged by size:
2120 1298 1148 677 245
NaeI GCC'GGC
Cuts at: 2101 2101
Size: 5488
NcoI C'CATG_G
Cuts at: 513 1065 2004 2567 513
Size: 552 939 563 3434
Fragments arranged by size:
3434 939 563 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5488
NgoMIV G'CCGG_C
Cuts at: 2099 2099
Size: 5488
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5488
NotI GC'GGCC_GC
Cuts at: 2935 2935
Size: 5488
NspI r_CATG'y
Cuts at: 1132 1229 2846 4391 1132
Size: 97 1617 1545 2229
Fragments arranged by size:
2229 1617 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5488
PciI A'CATG_T
Cuts at: 4387 4387
Size: 5488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5488
PmeI GTTT'AAAC
Cuts at: 2792 2792
Size: 5488
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3557 3589 5070 5102 3557
Size: 32 1481 32 3943
Fragments arranged by size:
3943 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2043 2713 2043
Size: 670 4818
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5488
PsiI TTA'TAA
Cuts at: 3058 3058
Size: 5488
PstI C_TGCA'G
Cuts at: 830 2526 2840 830
Size: 1696 314 3478
Fragments arranged by size:
3478 1696 314
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5488
SacI G_AGCT'C
Cuts at: 2693 2693
Size: 5488
SalI G'TCGA_C
Cuts at: 1936 2830 1936
Size: 894 4594
SanDI GG'GwC_CC
Cuts at: 2043 2043
Size: 5488
SbfI CC_TGCA'GG
Cuts at: 2840 2840
Size: 5488
ScaI AGT'ACT
Cuts at: 3736 5448 3736
Size: 1712 3776
SfcI C'TryA_G
Cuts at: 826 1045 2522 2836 3971 4652 4843 826
Size: 219 1477 314 1135 681 191 1471
Fragments arranged by size:
1477 1471 1135 681 314 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2105 2105
Size: 5488
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5488
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5488
SmaI CCC'GGG
Cuts at: 1872 2815 1872
Size: 943 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3592 4493 4755 820
Size: 197 926 1649 901 262 1553
Fragments arranged by size:
1649 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3279 493
Size: 2786 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5488
SphI G_CATG'C
Cuts at: 1132 2846 1132
Size: 1714 3774
SspI AAT'ATT
Cuts at: 5 52 3412 5
Size: 47 3360 2081
Fragments arranged by size:
3360 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5488
StyI C'CwwG_G
Cuts at: 513 1065 2004 2454 2567 2927 513
Size: 552 939 450 113 360 3074
Fragments arranged by size:
3074 939 552 450 360 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3515 3532 3685 3870 4315 325
Size: 1255 1935 17 153 185 445 1498
Fragments arranged by size:
1935 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3734 5446 96
Size: 274 80 33 51 1443 1757 1712 138
Fragments arranged by size:
1757 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5448 5480 5448
Size: 32 5456
Tth111I GACn'n_nGTC
Cuts at: 1316 2641 1316
Size: 1325 4163
XbaI T'CTAG_A
Cuts at: 2824 2824
Size: 5488
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5488
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5488
XmaI C'CCGG_G
Cuts at: 1870 2813 1870
Size: 943 4545
XmnI GAAnn'nnTTC
Cuts at: 3617 3617
Size: 5488
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2756 3296 276
Size: 53 83 186 936 1222 540 2468
Fragments arranged by size:
2468 1222 936 540 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SanDI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SapI SexAI SfoI SrfI
SwaI