Restriction map for
pFN21AB9549
(Circular) (Six-base) MAPSORT of: pFN21AB9549.seq Check: 5804 from: 1 to: 6109
pFN21AB9549 6109 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3246 3246
Size: 6109
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3919 278
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3452 1937
Size: 1515 4594
Acc65I G'GTAC_C
Cuts at: 3430 3430
Size: 6109
AclI AA'CG_TT
Cuts at: 1563 4236 4609 1563
Size: 2673 373 3063
Fragments arranged by size:
3063 2673 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 4172 5556 5824 1632
Size: 465 2075 1384 268 1917
Fragments arranged by size:
2075 1917 1384 465 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5912
AflIII A'CryG_T
Cuts at: 1203 2355 3220 3402 5008 5985 1203
Size: 1152 865 182 1606 977 1327
Fragments arranged by size:
1606 1327 1152 977 865 182
AgeI A'CCGG_T
Cuts at: 4940 4940
Size: 6109
AhdI GACnn_n'nnGTC
Cuts at: 4838 4838
Size: 6109
AleI CACnn'nnGTG
Cuts at: 1166 2358 2843 1166
Size: 1192 485 4432
Fragments arranged by size:
4432 1192 485
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6109
AlwNI CAG_nnn'CTG
Cuts at: 3852 5424 3852
Size: 1572 4537
ApaLI G'TGCA_C
Cuts at: 2836 4166 5322 2836
Size: 1330 1156 3623
Fragments arranged by size:
3623 1330 1156
ApoI r'AATT_y
Cuts at: 1493 2565 2870 3029 3418 3644 3796 5905 1493
Size: 1072 305 159 389 226 152 2109 1697
Fragments arranged by size:
2109 1697 1072 389 305 226 159 152
AseI AT'TA_AT
Cuts at: 160 1032 4663 160
Size: 872 3631 1606
Fragments arranged by size:
3631 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
AvaI C'yCGr_G
Cuts at: 1870 1943 2045 2679 3434 1870
Size: 73 102 634 755 4545
Fragments arranged by size:
4545 755 634 102 73
BamHI G'GATC_C
Cuts at: 3439 3806 3439
Size: 367 5742
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3430 4885 618
Size: 325 217 627 1643 1455 1842
Fragments arranged by size:
1842 1643 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2069 2237 2865 3428 1391
Size: 678 168 628 563 4072
Fragments arranged by size:
4072 678 628 563 168
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2765 2961 3356 928
Size: 971 866 196 395 3681
Fragments arranged by size:
3681 971 866 395 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4300 4334 1903
Size: 34 2363 34 3678
Fragments arranged by size:
3678 2363 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4001 5211 4001
Size: 1210 4899
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6109
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4720 136
Size: 107 122 71 631 605 3048 1525
Fragments arranged by size:
3048 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2537 2608 3390 6104 2537
Size: 71 782 2714 2542
Fragments arranged by size:
2714 2542 782 71
BlpI GC'TnA_GC
Cuts at: 3526 3526
Size: 6109
Bme1580I G_kGCm'C
Cuts at: 1790 2840 4170 5326 1790
Size: 1050 1330 1156 2573
Fragments arranged by size:
2573 1330 1156 1050
BmgBI CAC'GTC
Cuts at: 1318 2841 1318
Size: 1523 4586
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2280 3840 4798
Size: 416 53 83 399 24 863 1560 958
Cuts at: 4798 442
Size: 1753
Fragments arranged by size:
1753 1560 958 863 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6109
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4769 1239
Size: 3530 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 4234 5099 5397 2384
Size: 1850 865 298 3096
Fragments arranged by size:
3096 1850 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2843 4772 882
Size: 617 1344 1929 2219
Fragments arranged by size:
2219 1929 1344 617
BsaAI yAC'GTr
Cuts at: 493 1204 3900 493
Size: 711 2696 2702
Fragments arranged by size:
2702 2696 711
BsaBI GATnn'nnATC
Cuts at: 6103 6103
Size: 6109
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3916 4298 4933 275
Size: 53 83 186 936 2383 382 635 1451
Fragments arranged by size:
2383 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4542 4940 5214 5361 5772 4542
Size: 398 274 147 411 4879
Fragments arranged by size:
4879 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2729 2759 1178
Size: 30 165 30 1326 30 4528
Fragments arranged by size:
4528 1326 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 1186
Size: 1044 5065
BseYI C'CCAG_C
Cuts at: 5312 5808 5312
Size: 496 5613
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3356 1163
Size: 573 1620 3916
Fragments arranged by size:
3916 1620 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2425 3559 4320 5348 1540
Size: 275 186 424 1134 761 1028 2301
Fragments arranged by size:
2301 1134 1028 761 424 275 186
BsiHKAI G_wGCw'C
Cuts at: 2840 2865 3228 4170 4255 5326 2840
Size: 25 363 942 85 1071 3623
Fragments arranged by size:
3623 1071 942 363 85 25
BsmI GAATG_Cn'
Cuts at: 2535 3620 3713 2535
Size: 1085 93 4931
Fragments arranged by size:
4931 1085 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2237 2840 2865 3228 3428 4170
Size: 399 279 168 603 25 363 200 742
Cuts at: 4170 4255 5326 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 603 399 363 279 200
168 85 25
BspEI T'CCGG_A
Cuts at: 5772 5772
Size: 6109
BspHI T'CATG_A
Cuts at: 3998 5728 3998
Size: 1730 4379
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3246 3464 844
Size: 2402 218 3489
Fragments arranged by size:
3489 2402 218
BsrBI CCG'CTC
Cuts at: 1394 1823 3996 1394
Size: 429 2173 3507
Fragments arranged by size:
3507 2173 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2924 4604 4778 65
Size: 1183 1676 1680 174 1396
Fragments arranged by size:
1680 1676 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 4753 4940 1700
Size: 110 241 2702 187 2869
Fragments arranged by size:
2869 2702 241 187 110
BsrGI T'GTAC_A
Cuts at: 96 3217 96
Size: 3121 2988
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6109
BssSI C'ACGA_G
Cuts at: 3024 4169 5181 3024
Size: 1145 1012 3952
Fragments arranged by size:
3952 1145 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3334 794
Size: 2540 3569
BstBI TT'CG_AA
Cuts at: 4913 4913
Size: 6109
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6109
BstXI CCAn_nnnn'nTGG
Cuts at: 2779 2779
Size: 6109
BstYI r'GATC_y
Cuts at: 1972 2436 2537 2608 3390 3439 3806 4191 4208
Size: 464 101 71 782 49 367 385 17
Cuts at: 4208 5660 5746 5758 6104 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 782 464 385 367 346 101
86 71 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2332 3044 2332
Size: 712 5397
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4618
Fragments arranged by size:
4618 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5370
Fragments arranged by size:
5370 683 56
BtsI GCAGTG_nn'
Cuts at: 2824 3625 4419 4439 2824
Size: 801 794 20 4494
Fragments arranged by size:
4494 801 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6109
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6074
DraI TTT'AAA
Cuts at: 3413 3413
Size: 6109
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5116 809
Size: 507 3800 1802
Fragments arranged by size:
3800 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2622 2769 2781
Size: 54 1425 50 275 18 792 147 12
Cuts at: 2781 3556 4445 6074 8
Size: 775 889 1629 43
Fragments arranged by size:
1629 1425 889 792 775 275 147 54
50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 3556 1537
Size: 275 1744 4090
Fragments arranged by size:
4090 1744 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 3275 4039 1353
Size: 720 1202 764 3423
Fragments arranged by size:
3423 1202 764 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2822 4696 5070 5216 387
Size: 1064 138 1233 1874 374 146 1280
Fragments arranged by size:
1874 1280 1233 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2863 2863
Size: 6109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 4172 4769 5556 5824 1239
Size: 393 465 2075 597 787 268 1524
Fragments arranged by size:
2075 1524 787 597 465 393 268
EcoRI G'AATT_C
Cuts at: 2870 3029 3418 3796 2870
Size: 159 389 378 5183
Fragments arranged by size:
5183 389 378 159
EcoRV GAT'ATC
Cuts at: 3064 3127 3262 3064
Size: 63 135 5911
Fragments arranged by size:
5911 135 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 6109
FspI TGC'GCA
Cuts at: 3327 4615 3327
Size: 1288 4821
HaeII r_GCGC'y
Cuts at: 1678 5256 1678
Size: 3578 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2600 2632 4750
Size: 32 135 32 743 32 253 32 2118
Cuts at: 4750 4782 4824 4856 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2118 743 253 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3453 3699 669
Size: 988 281 1515 246 3079
Fragments arranged by size:
3079 1515 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2146 748
Size: 1398 4711
HpaI GTT'AAC
Cuts at: 3699 3699
Size: 6109
KpnI G_GTAC'C
Cuts at: 3434 3434
Size: 6109
MfeI C'AATT_G
Cuts at: 3708 3708
Size: 6109
MluI A'CGCG_T
Cuts at: 2355 5985 2355
Size: 3630 2479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3339 5223 5407 1305
Size: 2034 1884 184 2007
Fragments arranged by size:
2034 2007 1884 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2624 2771 2783 10
Size: 54 1425 343 792 147 12 3336
Fragments arranged by size:
3336 1425 792 343 147 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 2843 4067 4426 4585 518
Size: 648 1192 485 1224 359 159 2042
Fragments arranged by size:
2042 1224 1192 648 485 359 159
MspA1I CmG'CkG
Cuts at: 1606 2430 3310 3331 3525 4202 5350 5595 1606
Size: 824 880 21 194 677 1148 245 2120
Fragments arranged by size:
2120 1148 880 824 677 245 194 21
NaeI GCC'GGC
Cuts at: 2053 2053
Size: 6109
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4618
Fragments arranged by size:
4618 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6109
NgoMIV G'CCGG_C
Cuts at: 2051 2051
Size: 6109
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6109
NotI GC'GGCC_GC
Cuts at: 3556 3556
Size: 6109
NspI r_CATG'y
Cuts at: 1132 1229 3224 3326 3406 3467 5012 1132
Size: 97 1995 102 80 61 1545 2229
Fragments arranged by size:
2229 1995 1545 102 97 80 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6109
PciI A'CATG_T
Cuts at: 3220 3402 5008 3220
Size: 182 1606 4321
Fragments arranged by size:
4321 1606 182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6109
PmeI GTTT'AAAC
Cuts at: 3413 3413
Size: 6109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4178 4210 5691 5723 4178
Size: 32 1481 32 4564
Fragments arranged by size:
4564 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6109
PsiI TTA'TAA
Cuts at: 3020 3679 3020
Size: 659 5450
PspXI vC'TCGA_Gb
Cuts at: 2045 2045
Size: 6109
PstI C_TGCA'G
Cuts at: 830 2608 3243 3387 3461 830
Size: 1778 635 144 74 3478
Fragments arranged by size:
3478 1778 635 144 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6109
PvuII CAG'CTG
Cuts at: 2430 3310 3331 2430
Size: 880 21 5208
Fragments arranged by size:
5208 880 21
SacI G_AGCT'C
Cuts at: 2865 2865
Size: 6109
SalI G'TCGA_C
Cuts at: 1936 3451 1936
Size: 1515 4594
SbfI CC_TGCA'GG
Cuts at: 3461 3461
Size: 6109
ScaI AGT'ACT
Cuts at: 4357 6069 4357
Size: 1712 4397
SfcI C'TryA_G
Cuts at: 826 1045 2604 3239 3383 3394 3457 4592 5273
Size: 219 1559 635 144 11 63 1135 681
Cuts at: 5273 5464 826
Size: 191 1471
Fragments arranged by size:
1559 1471 1135 681 635 219 191 144
63 11
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6109
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6109
SmaI CCC'GGG
Cuts at: 1872 2681 3436 1872
Size: 809 755 4545
Fragments arranged by size:
4545 809 755
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2045 2399 4213 5114 5376 820
Size: 197 926 102 354 1814 901 262 1553
Fragments arranged by size:
1814 1553 926 901 354 262 197 102
SnaBI TAC'GTA
Cuts at: 493 3900 493
Size: 3407 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6109
SphI G_CATG'C
Cuts at: 1132 3326 3467 1132
Size: 2194 141 3774
Fragments arranged by size:
3774 2194 141
SspI AAT'ATT
Cuts at: 5 52 4033 5
Size: 47 3981 2081
Fragments arranged by size:
3981 2081 47
StuI AGG'CCT
Cuts at: 1517 2330 2642 1517
Size: 813 312 4984
Fragments arranged by size:
4984 813 312
StyI C'CwwG_G
Cuts at: 513 1065 2004 2877 3548 513
Size: 552 939 873 671 3074
Fragments arranged by size:
3074 939 873 671 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2191 4136 4153 4306 4491 4936 325
Size: 1255 611 1945 17 153 185 445 1498
Fragments arranged by size:
1945 1498 1255 611 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3217 4355 6067
Size: 274 80 33 51 1443 1240 1138 1712
Cuts at: 6067 96
Size: 138
Fragments arranged by size:
1712 1443 1240 1138 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2729 2761 6069 6101 2729
Size: 32 3308 32 2737
Fragments arranged by size:
3308 2737 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3355 1316
Size: 2039 4070
XbaI T'CTAG_A
Cuts at: 2059 3445 2059
Size: 1386 4723
XhoI C'TCGA_G
Cuts at: 1943 2045 1943
Size: 102 6007
XmaI C'CCGG_G
Cuts at: 1870 2679 3434 1870
Size: 809 755 4545
Fragments arranged by size:
4545 809 755
XmnI GAAnn'nnTTC
Cuts at: 2654 3053 4238 2654
Size: 399 1185 4525
Fragments arranged by size:
4525 1185 399
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3917 276
Size: 53 83 186 936 2383 2468
Fragments arranged by size:
2468 2383 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI
EcoO109I FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI