Restriction map for
pFN21AB9542
(Circular) (Six-base) MAPSORT of: pFN21AB9542.seq Check: 6662 from: 1 to: 5575
pFN21AB9542 5575 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3385 278
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2279 2673 2799 3638 5022 5290 1632
Size: 437 210 394 126 839 1384 268 1917
Fragments arranged by size:
1917 1384 839 437 394 268 210 126
AfeI AGC'GCT
Cuts at: 2523 2523
Size: 5575
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 2138 2503 4474 5451 1203
Size: 935 365 1971 977 1327
Fragments arranged by size:
1971 1327 977 935 365
AgeI A'CCGG_T
Cuts at: 2796 4406 2796
Size: 1610 3965
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5575
AlwNI CAG_nnn'CTG
Cuts at: 2650 3318 4890 2650
Size: 668 1572 3335
Fragments arranged by size:
3335 1572 668
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2884 3110 3262 5371 1493
Size: 1391 226 152 2109 1697
Fragments arranged by size:
2109 1697 1391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2157 2210 4129 160
Size: 872 1125 53 1919 1606
Fragments arranged by size:
1919 1606 1125 872 53
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2900 1870
Size: 73 957 4545
Fragments arranged by size:
4545 957 73
BamHI G'GATC_C
Cuts at: 2905 3272 2905
Size: 367 5208
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2768 2896 4351 618
Size: 325 217 627 981 128 1455 1842
Fragments arranged by size:
1842 1455 981 627 325 217 128
BanII G_rGCy'C
Cuts at: 1391 2894 1391
Size: 1503 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3766 3800 1903
Size: 34 50 34 1745 34 3678
Fragments arranged by size:
3678 1745 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2404 4186 136
Size: 107 122 71 631 605 732 1782 1525
Fragments arranged by size:
1782 1525 732 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2479 5570 2479
Size: 3091 2484
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4792 1790
Size: 1846 1156 2573
Fragments arranged by size:
2573 1846 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3306 4264 442
Size: 416 53 83 399 24 1889 958 1753
Fragments arranged by size:
1889 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2735 4235 1239
Size: 1496 1500 2579
Fragments arranged by size:
2579 1500 1496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2504 2828 3700 4565 4863 2504
Size: 324 872 865 298 3216
Fragments arranged by size:
3216 872 865 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2022 2630 4238 882
Size: 617 523 608 1608 2219
Fragments arranged by size:
2219 1608 617 608 523
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3382 3764 4399 275
Size: 53 83 186 936 1849 382 635 1451
Fragments arranged by size:
1849 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2796 4008 4406 4680 4827 5238 2796
Size: 1212 398 274 147 411 3133
Fragments arranged by size:
3133 1212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5350
Fragments arranged by size:
5350 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2846 1186
Size: 1660 3915
BseYI C'CCAG_C
Cuts at: 2033 2164 2352 2719 4778 5274 2033
Size: 131 188 367 2059 496 2334
Fragments arranged by size:
2334 2059 496 367 188 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2788 3636 3721 4792 2788
Size: 848 85 1071 3571
Fragments arranged by size:
3571 1071 848 85
BsmI GAATG_Cn'
Cuts at: 3086 3179 3086
Size: 93 5482
BsmBI CGTCTCn'nnnn_
Cuts at: 2017 2017
Size: 5575
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2788 2894 3636 3721 4792 1391
Size: 399 998 106 742 85 1071 2174
Fragments arranged by size:
2174 1071 998 742 399 106 85
BspEI T'CCGG_A
Cuts at: 5238 5238
Size: 5575
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2930 844
Size: 2086 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2107 2796 4219 4406 1700
Size: 110 297 689 1423 187 2869
Fragments arranged by size:
2869 1423 689 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 2828 3635 4647 2828
Size: 807 1012 3756
Fragments arranged by size:
3756 1012 807
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 2639 1184
Size: 1455 4120
BstXI CCAn_nnnn'nTGG
Cuts at: 2537 2679 2537
Size: 142 5433
BstYI r'GATC_y
Cuts at: 1972 2479 2905 3272 3657 3674 5126 5212 5224
Size: 507 426 367 385 17 1452 86 12
Cuts at: 5224 5570 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 507 426 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2620 513
Size: 552 1555 3468
Fragments arranged by size:
3468 1555 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DraIII CAC_nnn'GTG
Cuts at: 2662 2662
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3022 3911 5540
Size: 54 1425 50 275 18 1192 889 1629
Cuts at: 5540 8
Size: 43
Fragments arranged by size:
1629 1425 1192 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2306 3505 1353
Size: 953 1199 3423
Fragments arranged by size:
3423 1199 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2462 4162 4536 4682 387
Size: 1064 138 873 1700 374 146 1280
Fragments arranged by size:
1700 1280 1064 873 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2279 2673 2735 2799 3638 4235
Size: 393 437 210 394 62 64 839 597
Cuts at: 4235 5022 5290 1239
Size: 787 268 1524
Fragments arranged by size:
1524 839 787 597 437 394 393 268
210 64 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2421 2421
Size: 5575
EcoO109I rG'GnC_Cy
Cuts at: 2694 2694
Size: 5575
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 2525 4722 1678
Size: 847 2197 2531
Fragments arranged by size:
2531 2197 847
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2049 2081 4216 4248 4290
Size: 32 135 32 477 32 2135 32 42
Cuts at: 4290 4322 1373
Size: 32 2626
Fragments arranged by size:
2626 2135 477 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 2237 748
Size: 1489 4086
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2080 2580 4689 4873 1305
Size: 775 500 2109 184 2007
Fragments arranged by size:
2109 2007 775 500 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2281 3533 3892 4051 518
Size: 648 960 155 1252 359 159 2042
Fragments arranged by size:
2042 1252 960 648 359 159 155
MspA1I CmG'CkG
Cuts at: 1606 2991 3668 4816 5061 1606
Size: 1385 677 1148 245 2120
Fragments arranged by size:
2120 1385 1148 677 245
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5575
NcoI C'CATG_G
Cuts at: 513 1065 2620 513
Size: 552 1555 3468
Fragments arranged by size:
3468 1555 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2142 2507 2933 4478 1132
Size: 97 913 365 426 1545 2229
Fragments arranged by size:
2229 1545 913 426 365 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 2138 2503 4474 2138
Size: 365 1971 3239
Fragments arranged by size:
3239 1971 365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2124 2662 1108
Size: 1016 538 4021
Fragments arranged by size:
4021 1016 538
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2694 2694
Size: 5575
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2431 2463 2431
Size: 32 5543
PstI C_TGCA'G
Cuts at: 830 2422 2927 830
Size: 1592 505 3478
Fragments arranged by size:
3478 1592 505
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SanDI GG'GwC_CC
Cuts at: 2694 2694
Size: 5575
SbfI CC_TGCA'GG
Cuts at: 2422 2927 2422
Size: 505 5070
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2418 2923 4058 4739 4930 826
Size: 219 1373 505 1135 681 191 1471
Fragments arranged by size:
1471 1373 1135 681 505 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2483 2843 3679 4580 4842 820
Size: 197 926 540 360 836 901 262 1553
Fragments arranged by size:
1553 926 901 836 540 360 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2933 1132
Size: 1801 3774
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 2416 1517
Size: 899 4676
StyI C'CwwG_G
Cuts at: 513 1065 2620 3014 513
Size: 552 1555 394 3074
Fragments arranged by size:
3074 1555 552 394
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2025 2366 2531 3602 3619 3772 3957
Size: 1255 445 341 165 1071 17 153 185
Cuts at: 3957 4402 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1071 445 445 341 185 165
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2242 2839 3821
Size: 274 80 33 51 1443 265 597 982
Cuts at: 3821 5533 96
Size: 1712 138
Fragments arranged by size:
1712 1443 982 597 274 265 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5535 5567 5535
Size: 32 5543
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5575
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2125 2125
Size: 5575
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3383 276
Size: 53 83 186 936 1849 2468
Fragments arranged by size:
2468 1849 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PvuII RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI SwaI