Restriction map for
pFN21AB9520
(Circular) (Six-base) MAPSORT of: pFN21AB9520.seq Check: 7549 from: 1 to: 6040
pFN21AB9520 6040 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4103 5487 5755 1632
Size: 2471 1384 268 1917
Fragments arranged by size:
2471 1917 1384 268
AfeI AGC'GCT
Cuts at: 3044 3044
Size: 6040
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 4939 5916 1203
Size: 3736 977 1327
Fragments arranged by size:
3736 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AlwNI CAG_nnn'CTG
Cuts at: 3783 5355 3783
Size: 1572 4468
ApaI G_GGCC'C
Cuts at: 2437 3121 2437
Size: 684 5356
ApaLI G'TGCA_C
Cuts at: 2356 4097 5253 2356
Size: 1741 1156 3143
Fragments arranged by size:
3143 1741 1156
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2191 2415 2555 2712 2729 2741 2876
Size: 73 248 224 140 157 17 12 135
Cuts at: 2876 2935 3057 3365 1870
Size: 59 122 308 4545
Fragments arranged by size:
4545 308 248 224 157 140 135 122
73 59 17 12
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 3361 4816 618
Size: 325 217 627 468 1106 1455 1842
Fragments arranged by size:
1842 1455 1106 627 468 325 217
BanII G_rGCy'C
Cuts at: 1391 2437 2713 2736 2975 3111 3121 3359 1391
Size: 1046 276 23 239 136 10 238 4072
Fragments arranged by size:
4072 1046 276 239 238 136 23 10
BbeI G_GCGC'C
Cuts at: 2259 2259
Size: 6040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5069
BbvCI CC'TCA_GC
Cuts at: 2639 2660 2856 2639
Size: 21 196 5823
Fragments arranged by size:
5823 196 21
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4231 4265 1903
Size: 34 2294 34 3678
Fragments arranged by size:
3678 2294 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 3138 1548
Size: 1590 4450
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2026 2183 2867
Size: 107 122 71 631 605 354 157 684
Cuts at: 2867 3218 4651 136
Size: 351 1433 1525
Fragments arranged by size:
1525 1433 684 631 605 354 351 157
122 107 71
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2360 2437 2802 2945 3121 4101 5257 1790
Size: 570 77 365 143 176 980 1156 2573
Fragments arranged by size:
2573 1156 980 570 365 176 143 77
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 4729 442
Size: 416 53 83 399 24 2354 958 1753
Fragments arranged by size:
2354 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 2902 3023 3344 4700 1239
Size: 1400 263 121 321 1356 2579
Fragments arranged by size:
2579 1400 1356 321 263 121
Bpu10I CC'TnA_GC
Cuts at: 2519 2639 2660 2856 2519
Size: 120 21 196 5703
Fragments arranged by size:
5703 196 120 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2948 4165 5030 5328 2948
Size: 1217 865 298 3660
Fragments arranged by size:
3660 1217 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2428 4703 882
Size: 617 929 2275 2219
Fragments arranged by size:
2275 2219 929 617
BsaAI yAC'GTr
Cuts at: 493 1204 2287 3831 493
Size: 711 1083 1544 2702
Fragments arranged by size:
2702 1544 1083 711
BsaBI GATnn'nnATC
Cuts at: 3140 6034 3140
Size: 2894 3146
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2256 2987 3847 4229
Size: 53 83 186 936 723 731 860 382
Cuts at: 4229 4864 275
Size: 635 1451
Fragments arranged by size:
1451 936 860 731 723 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2519 2549 3176 3206 1178
Size: 30 165 30 1116 30 627 30 4012
Fragments arranged by size:
4012 1116 627 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2353 2923 1186
Size: 1167 570 4303
Fragments arranged by size:
4303 1167 570
BseYI C'CCAG_C
Cuts at: 2152 2436 2523 2748 2778 3039 3114 5243 5739
Size: 284 87 225 30 261 75 2129 496
Cuts at: 5739 2152
Size: 2453
Fragments arranged by size:
2453 2129 496 284 261 225 87 75
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 1163
Size: 573 1068 4399
Fragments arranged by size:
4399 1068 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2314 2398 2847 3490 4251 5279
Size: 275 186 313 84 449 643 761 1028
Cuts at: 5279 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 643 449 313 275 186
84
BsiHKAI G_wGCw'C
Cuts at: 2360 2385 3330 4101 4186 5257 2360
Size: 25 945 771 85 1071 3143
Fragments arranged by size:
3143 1071 945 771 85 25
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2360 2385 2437 2713 2736 2802 2945
Size: 399 570 25 52 276 23 66 143
Cuts at: 2945 2975 3111 3121 3330 3359 4101 4186 5257
Size: 30 136 10 209 29 742 85 1071
Cuts at: 5257 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 570 399 276 209 143
136 85 66 52 30 29 25 23
10
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 3929 5659 3929
Size: 1730 4310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 3395 844
Size: 1514 1037 3489
Fragments arranged by size:
3489 1514 1037
BsrBI CCG'CTC
Cuts at: 1394 1823 2420 3927 1394
Size: 429 597 1507 3507
Fragments arranged by size:
3507 1507 597 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2723 4684 4871 1700
Size: 110 913 1961 187 2869
Fragments arranged by size:
2869 1961 913 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 2011 2013 2081 1925
Size: 86 2 68 5884
Fragments arranged by size:
5884 86 68 2
BssSI C'ACGA_G
Cuts at: 4100 5112 4100
Size: 1012 5028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 2410 1184
Size: 1226 4814
BstXI CCAn_nnnn'nTGG
Cuts at: 2152 3082 2152
Size: 930 5110
BstYI r'GATC_y
Cuts at: 1972 3370 3737 4122 4139 5591 5677 5689 6035
Size: 1398 367 385 17 1452 86 12 346
Cuts at: 6035 1972
Size: 1977
Fragments arranged by size:
1977 1452 1398 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2106 2145 2901 3157 513
Size: 552 939 102 39 756 256 3396
Fragments arranged by size:
3396 939 756 552 256 102 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 2563 3163 3328 3556 4350 4370 2466
Size: 97 600 165 228 794 20 4136
Fragments arranged by size:
4136 794 600 228 165 97 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2249 5047 809
Size: 507 933 2798 1802
Fragments arranged by size:
2798 1802 933 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2142 2184
Size: 54 1425 50 275 18 285 27 42
Cuts at: 2184 2311 2844 3072 3084 3160 3487 4376 6005
Size: 127 533 228 12 76 327 889 1629
Cuts at: 6005 8
Size: 43
Fragments arranged by size:
1629 1425 889 533 327 285 275 228
127 76 54 50 43 42 27 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 2311 2844 3487 1537
Size: 275 499 533 643 4090
Fragments arranged by size:
4090 643 533 499 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3970 1353
Size: 2617 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3251 4627 5001 5147 387
Size: 1064 138 1662 1376 374 146 1280
Fragments arranged by size:
1662 1376 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2639 2902 3023 3344 4103 4700 5487
Size: 393 1007 263 121 321 759 597 787
Cuts at: 5487 5755 1239
Size: 268 1524
Fragments arranged by size:
1524 1007 787 759 597 393 321 268
263 121
EcoO109I rG'GnC_Cy
Cuts at: 2317 3118 2317
Size: 801 5239
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 2051 2132 2259 2856 3046 5187 1678
Size: 373 81 127 597 190 2141 2531
Fragments arranged by size:
2531 2141 597 373 190 127 81
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2499 2519 2531 2551 4681
Size: 32 135 32 927 20 12 20 2130
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2130 927 135 42 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3226 3384 3630 669
Size: 988 281 1288 158 246 3079
Fragments arranged by size:
3079 1288 988 281 246 158
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KasI G'GCGC_C
Cuts at: 2255 2255
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2265 5154 5338 1305
Size: 960 2889 184 2007
Fragments arranged by size:
2889 2007 960 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2144 3074 3162 10
Size: 54 1425 343 312 930 88 2888
Fragments arranged by size:
2888 1425 930 343 312 88 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2568 2826 3017 3998 4357 4516 518
Size: 648 1402 258 191 981 359 159 2042
Fragments arranged by size:
2042 1402 981 648 359 258 191 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2108 2301 2818 3456 4133 5281 5526
Size: 426 76 193 517 638 677 1148 245
Cuts at: 5526 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 638 517 426 245 193
76
NaeI GCC'GGC
Cuts at: 2725 2725
Size: 6040
NarI GG'CG_CC
Cuts at: 2256 2256
Size: 6040
NcoI C'CATG_G
Cuts at: 513 1065 2004 3157 513
Size: 552 939 1153 3396
Fragments arranged by size:
3396 1153 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NgoMIV G'CCGG_C
Cuts at: 2723 2723
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 3398 4943 1132
Size: 97 2169 1545 2229
Fragments arranged by size:
2229 2169 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2771 1108
Size: 1663 4377
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PspOMI G'GGCC_C
Cuts at: 2433 3117 2433
Size: 684 5356
PspXI vC'TCGA_Gb
Cuts at: 2191 2935 2191
Size: 744 5296
PstI C_TGCA'G
Cuts at: 830 3392 830
Size: 2562 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
SacII CC_GC'GG
Cuts at: 2109 2109
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 3388 4523 5204 5395 826
Size: 219 2343 1135 681 191 1471
Fragments arranged by size:
2343 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2257 2257
Size: 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2191 2712 2935 2963 4144 5045
Size: 197 926 248 521 223 28 1181 901
Cuts at: 5045 5307 820
Size: 262 1553
Fragments arranged by size:
1553 1181 926 901 521 262 248 223
197 28
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2004 3157 3479 513
Size: 552 939 1153 322 3074
Fragments arranged by size:
3074 1153 939 552 322
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 2398 2473 2825 2926 3053 4067
Size: 1255 639 179 75 352 101 127 1014
Cuts at: 4067 4084 4237 4422 4867 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1014 639 445 352 185 179
153 127 101 75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4286 5998 96
Size: 274 80 33 51 1443 2309 1712 138
Fragments arranged by size:
2309 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 2191 2712 2935 1943
Size: 248 521 223 5048
Fragments arranged by size:
5048 521 248 223
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 2213 4169 2213
Size: 1956 4084
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI
PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI
SrfI SwaI XcmI