Restriction map for
pFN21AB9517
(Circular) (Six-base) MAPSORT of: pFN21AB9517.seq Check: 9981 from: 1 to: 6400
pFN21AB9517 6400 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3241 3241
Size: 6400
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4210 278
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3743 1937
Size: 1806 4594
Acc65I G'GTAC_C
Cuts at: 3721 3721
Size: 6400
AclI AA'CG_TT
Cuts at: 1563 4527 4900 1563
Size: 2964 373 3063
Fragments arranged by size:
3063 2964 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 3377 3599 4463 5847 6115 1632
Size: 531 1214 222 864 1384 268 1917
Fragments arranged by size:
1917 1384 1214 864 531 268 222
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6203
AflIII A'CryG_T
Cuts at: 1203 2076 2760 5299 6276 1203
Size: 873 684 2539 977 1327
Fragments arranged by size:
2539 1327 977 873 684
AgeI A'CCGG_T
Cuts at: 2982 5231 2982
Size: 2249 4151
AhdI GACnn_n'nnGTC
Cuts at: 3131 5129 3131
Size: 1998 4402
AleI CACnn'nnGTG
Cuts at: 1166 2486 3492 1166
Size: 1320 1006 4074
Fragments arranged by size:
4074 1320 1006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6400
AlwNI CAG_nnn'CTG
Cuts at: 2293 3041 3537 3550 4143 5715 2293
Size: 748 496 13 593 1572 2978
Fragments arranged by size:
2978 1572 748 593 496 13
ApaI G_GGCC'C
Cuts at: 2071 3409 3465 2071
Size: 1338 56 5006
Fragments arranged by size:
5006 1338 56
ApaLI G'TGCA_C
Cuts at: 2764 3647 4457 5613 2764
Size: 883 810 1156 3551
Fragments arranged by size:
3551 1156 883 810
ApoI r'AATT_y
Cuts at: 1493 2912 3709 3935 4087 6196 1493
Size: 1419 797 226 152 2109 1697
Fragments arranged by size:
2109 1697 1419 797 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4954 160
Size: 872 3922 1606
Fragments arranged by size:
3922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 2226 2639 3725 1870
Size: 73 283 413 1086 4545
Fragments arranged by size:
4545 1086 413 283 73
BamHI G'GATC_C
Cuts at: 2941 3730 4097 2941
Size: 789 367 5244
Fragments arranged by size:
5244 789 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2493 2547 2635 3721 5176
Size: 325 217 627 706 54 88 1086 1455
Cuts at: 5176 618
Size: 1842
Fragments arranged by size:
1842 1455 1086 706 627 325 217 88
54
BanII G_rGCy'C
Cuts at: 1391 2071 2151 3409 3465 3719 1391
Size: 680 80 1258 56 254 4072
Fragments arranged by size:
4072 1258 680 254 80 56
BbeI G_GCGC'C
Cuts at: 2551 2551
Size: 6400
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3398 3587 928
Size: 971 1499 189 3741
Fragments arranged by size:
3741 1499 971 189
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4591 4625 1903
Size: 34 2654 34 3678
Fragments arranged by size:
3678 2654 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4292 5502 4292
Size: 1210 5190
BclI T'GATC_A
Cuts at: 1548 3195 1548
Size: 1647 4753
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5011 136
Size: 107 122 71 631 605 3339 1525
Fragments arranged by size:
3339 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6395 6395
Size: 6400
BlpI GC'TnA_GC
Cuts at: 2840 3817 2840
Size: 977 5423
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2640 2768 3409 3465 3475 3651 4461
Size: 281 569 128 641 56 10 176 810
Cuts at: 4461 5617 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 810 641 569 281 176 128
56 10
BmgBI CAC'GTC
Cuts at: 1318 2424 3655 1318
Size: 1106 1231 4063
Fragments arranged by size:
4063 1231 1106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2430 4131 5089
Size: 416 53 83 399 24 1013 1701 958
Cuts at: 5089 442
Size: 1753
Fragments arranged by size:
1753 1701 1013 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2135 5060 1239
Size: 896 2925 2579
Fragments arranged by size:
2925 2579 896
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3314 4525 5390 5688 3314
Size: 1211 865 298 4026
Fragments arranged by size:
4026 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 5063 882
Size: 617 609 2955 2219
Fragments arranged by size:
2955 2219 617 609
BsaAI yAC'GTr
Cuts at: 493 1204 2813 3588 4191 493
Size: 711 1609 775 603 2702
Fragments arranged by size:
2702 1609 775 711 603
BsaBI GATnn'nnATC
Cuts at: 2669 6394 2669
Size: 3725 2675
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2548 4207 4589 5224
Size: 53 83 186 936 1015 1659 382 635
Cuts at: 5224 275
Size: 1451
Fragments arranged by size:
1659 1451 1015 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2982 4833 5231 5505 5652 6063 2982
Size: 1851 398 274 147 411 3319
Fragments arranged by size:
3319 1851 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2088 2118 2348 2378 3092
Size: 30 165 30 685 30 230 30 714
Cuts at: 3092 3122 1178
Size: 30 4456
Fragments arranged by size:
4456 714 685 230 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2175 2356 2612 2743 2798 2831 3113 3224
Size: 989 181 256 131 55 33 282 111
Cuts at: 3224 3260 3359 3455 1186
Size: 36 99 96 4131
Fragments arranged by size:
4131 989 282 256 181 131 111 99
96 55 36 33
BseYI C'CCAG_C
Cuts at: 2109 2234 3450 5603 6099 2109
Size: 125 1216 2153 496 2410
Fragments arranged by size:
2410 2153 1216 496 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3302 3404 3516 1163
Size: 573 1566 102 112 4047
Fragments arranged by size:
4047 1566 573 112 102
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3144 3850 4611 5639 1540
Size: 275 186 1143 706 761 1028 2301
Fragments arranged by size:
2301 1143 1028 761 706 275 186
BsiHKAI G_wGCw'C
Cuts at: 2313 2768 3177 3651 4461 4546 5617 2313
Size: 455 409 474 810 85 1071 3096
Fragments arranged by size:
3096 1071 810 474 455 409 85
BsiWI C'GTAC_G
Cuts at: 3258 3258
Size: 6400
BsmI GAATG_Cn'
Cuts at: 3911 4004 3911
Size: 93 6307
BsmBI CGTCTCn'nnnn_
Cuts at: 2368 2368
Size: 6400
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2151 2313 2640 2768 3177 3409
Size: 399 281 80 162 327 128 409 232
Cuts at: 3409 3465 3475 3651 3719 4461 4546 5617 1391
Size: 56 10 176 68 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 327 281 232
176 162 128 85 80 68 56 10
BspEI T'CCGG_A
Cuts at: 6063 6063
Size: 6400
BspHI T'CATG_A
Cuts at: 2875 3192 3657 4289 6019 2875
Size: 317 465 632 1730 3256
Fragments arranged by size:
3256 1730 632 465 317
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2844 3241 3755 844
Size: 2000 397 514 3489
Fragments arranged by size:
3489 2000 514 397
BsrBI CCG'CTC
Cuts at: 1394 1823 4287 1394
Size: 429 2464 3507
Fragments arranged by size:
3507 2464 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3522 4895 5069 65
Size: 1183 2274 1373 174 1396
Fragments arranged by size:
2274 1396 1373 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2982 3309 3512 5044 5231 1700
Size: 110 1172 327 203 1532 187 2869
Fragments arranged by size:
2869 1532 1172 327 203 187 110
BsrGI T'GTAC_A
Cuts at: 96 3108 96
Size: 3012 3388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6400
BssSI C'ACGA_G
Cuts at: 4460 5472 4460
Size: 1012 5388
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2268 3427 3540 794
Size: 1474 1159 113 3654
Fragments arranged by size:
3654 1474 1159 113
BstBI TT'CG_AA
Cuts at: 5204 5204
Size: 6400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6400
BstYI r'GATC_y
Cuts at: 1972 2203 2941 3730 4097 4482 4499 5951 6037
Size: 231 738 789 367 385 17 1452 86
Cuts at: 6037 6049 6395 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 789 738 385 367 346 231
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2501 2538 2679 2501
Size: 37 141 6222
Fragments arranged by size:
6222 141 37
BtgI C'CryG_G
Cuts at: 513 1065 2400 2460 2712 513
Size: 552 1335 60 252 4201
Fragments arranged by size:
4201 1335 552 252 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3097 487
Size: 683 56 1871 3790
Fragments arranged by size:
3790 1871 683 56
BtsI GCAGTG_nn'
Cuts at: 2092 2528 3487 3499 3916 4710 4730 2092
Size: 436 959 12 417 794 20 3762
Fragments arranged by size:
3762 959 794 436 417 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6365
DraI TTT'AAA
Cuts at: 3704 3704
Size: 6400
DraIII CAC_nnn'GTG
Cuts at: 3427 3427
Size: 6400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5407 809
Size: 507 4091 1802
Fragments arranged by size:
4091 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2329 3239 3847
Size: 54 1425 50 275 18 499 910 608
Cuts at: 3847 4736 6365 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 910 889 608 499 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3847 1537
Size: 275 2035 4090
Fragments arranged by size:
4090 2035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2862 4330 1353
Size: 1509 1468 3423
Fragments arranged by size:
3423 1509 1468
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2812 2924 4987 5361 5507 387
Size: 1064 138 1223 112 2063 374 146 1280
Fragments arranged by size:
2063 1280 1223 1064 374 146 138 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2135 2163 3377 3599 4463 5060 5847
Size: 393 503 28 1214 222 864 597 787
Cuts at: 5847 6115 1239
Size: 268 1524
Fragments arranged by size:
1524 1214 864 787 597 503 393 268
222 28
EcoNI CCTnn'n_nnAGG
Cuts at: 2903 2903
Size: 6400
EcoO109I rG'GnC_Cy
Cuts at: 2067 2068 2884 3125 3406 3461 3462 2067
Size: 1 816 241 281 55 1 5005
Fragments arranged by size:
5005 816 281 241 55 1 1
EcoRI G'AATT_C
Cuts at: 2912 3709 4087 2912
Size: 797 378 5225
Fragments arranged by size:
5225 797 378
FspI TGC'GCA
Cuts at: 4906 4906
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 2235 2551 2847 5547 1678
Size: 557 316 296 2700 2531
Fragments arranged by size:
2700 2531 557 316 296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2678 2710 3117 3149 5041
Size: 32 135 32 1106 32 407 32 1892
Cuts at: 5041 5073 5115 5147 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1892 1106 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3744 3990 669
Size: 988 281 1806 246 3079
Fragments arranged by size:
3079 1806 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6400
HpaI GTT'AAC
Cuts at: 3990 3990
Size: 6400
KasI G'GCGC_C
Cuts at: 2547 2547
Size: 6400
KpnI G_GTAC'C
Cuts at: 3725 3725
Size: 6400
MfeI C'AATT_G
Cuts at: 3999 3999
Size: 6400
MluI A'CGCG_T
Cuts at: 6276 6276
Size: 6400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5514 5698 1305
Size: 4209 184 2007
Fragments arranged by size:
4209 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2331 10
Size: 54 1425 343 499 4079
Fragments arranged by size:
4079 1425 499 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2486 3191 3492 4358 4717 4876 518
Size: 648 1320 705 301 866 359 159 2042
Fragments arranged by size:
2042 1320 866 705 648 359 301 159
MspA1I CmG'CkG
Cuts at: 1606 2322 2392 3550 3679 3816 4493 5641 5886
Size: 716 70 1158 129 137 677 1148 245
Cuts at: 5886 1606
Size: 2120
Fragments arranged by size:
2120 1158 1148 716 677 245 137 129
70
NarI GG'CG_CC
Cuts at: 2548 2548
Size: 6400
NcoI C'CATG_G
Cuts at: 513 1065 2712 513
Size: 552 1647 4201
Fragments arranged by size:
4201 1647 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6400
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6400
NotI GC'GGCC_GC
Cuts at: 3847 3847
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 2080 2764 3630 3758 5303 1132
Size: 97 851 684 866 128 1545 2229
Fragments arranged by size:
2229 1545 866 851 684 128 97
PasI CC'CwG_GG
Cuts at: 1348 2963 1348
Size: 1615 4785
PciI A'CATG_T
Cuts at: 2076 2760 5299 2076
Size: 684 2539 3177
Fragments arranged by size:
3177 2539 684
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2406 1108
Size: 1298 5102
PfoI T'CCnGG_A
Cuts at: 2151 2619 2151
Size: 468 5932
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PmlI CAC'GTG
Cuts at: 2813 3588 2813
Size: 775 5625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2719 2751 4469 4501 5982 6014 2719
Size: 32 1718 32 1481 32 3105
Fragments arranged by size:
3105 1718 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3125 3125
Size: 6400
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6400
PsiI TTA'TAA
Cuts at: 3970 3970
Size: 6400
PspOMI G'GGCC_C
Cuts at: 2067 3405 3461 2067
Size: 1338 56 5006
Fragments arranged by size:
5006 1338 56
PspXI vC'TCGA_Gb
Cuts at: 2226 2226
Size: 6400
PstI C_TGCA'G
Cuts at: 830 2087 2724 2907 3494 3752 830
Size: 1257 637 183 587 258 3478
Fragments arranged by size:
3478 1257 637 587 258 183
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
PvuII CAG'CTG
Cuts at: 2392 3550 3679 2392
Size: 1158 129 5113
Fragments arranged by size:
5113 1158 129
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SanDI GG'GwC_CC
Cuts at: 3125 3125
Size: 6400
SapI GCTCTTCn'nnn_
Cuts at: 2862 2862
Size: 6400
SbfI CC_TGCA'GG
Cuts at: 3752 3752
Size: 6400
ScaI AGT'ACT
Cuts at: 4648 6360 4648
Size: 1712 4688
SexAI A'CCwGG_T
Cuts at: 3423 3423
Size: 6400
SfcI C'TryA_G
Cuts at: 826 1045 2083 2720 2903 3490 3748 4883 5564
Size: 219 1038 637 183 587 258 1135 681
Cuts at: 5564 5755 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1038 681 637 587 258 219
191 183
SfoI GGC'GCC
Cuts at: 2549 2549
Size: 6400
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 3727 1872
Size: 1855 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 3329 4504 5405 5667 820
Size: 197 926 283 1103 1175 901 262 1553
Fragments arranged by size:
1553 1175 1103 926 901 283 262 197
SnaBI TAC'GTA
Cuts at: 493 4191 493
Size: 3698 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SspI AAT'ATT
Cuts at: 5 52 4324 5
Size: 47 4272 2081
Fragments arranged by size:
4272 2081 47
StuI AGG'CCT
Cuts at: 1517 3354 1517
Size: 1837 4563
StyI C'CwwG_G
Cuts at: 513 1065 2712 3839 513
Size: 552 1647 1127 3074
Fragments arranged by size:
3074 1647 1127 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2536 4427 4444 4597 4782 5227 325
Size: 1255 956 1891 17 153 185 445 1498
Fragments arranged by size:
1891 1498 1255 956 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3108 4646 6358
Size: 274 80 33 51 1443 1131 1538 1712
Cuts at: 6358 96
Size: 138
Fragments arranged by size:
1712 1538 1443 1131 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6360 6392 6360
Size: 32 6368
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6400
XbaI T'CTAG_A
Cuts at: 3736 3736
Size: 6400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2443 2935 2443
Size: 492 5908
XhoI C'TCGA_G
Cuts at: 1943 2226 1943
Size: 283 6117
XmaI C'CCGG_G
Cuts at: 1870 3725 1870
Size: 1855 4545
XmnI GAAnn'nnTTC
Cuts at: 2871 4529 2871
Size: 1658 4742
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4208 276
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PsrI RsrII SacI
SacII SfiI SrfI SwaI