Restriction map for
pFN21AB9513
(Circular) (Six-base) MAPSORT of: pFN21AB9513.seq Check: 6814 from: 1 to: 6145
pFN21AB9513 6145 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3955 278
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2102 3488 1937
Size: 165 1386 4594
Fragments arranged by size:
4594 1386 165
Acc65I G'GTAC_C
Cuts at: 2304 3466 2304
Size: 1162 4983
AclI AA'CG_TT
Cuts at: 1563 4272 4645 1563
Size: 2709 373 3063
Fragments arranged by size:
3063 2709 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4208 5592 5860 1632
Size: 2576 1384 268 1917
Fragments arranged by size:
2576 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5948
AflIII A'CryG_T
Cuts at: 1203 2409 3381 5044 6021 1203
Size: 1206 972 1663 977 1327
Fragments arranged by size:
1663 1327 1206 977 972
AgeI A'CCGG_T
Cuts at: 4976 4976
Size: 6145
AhdI GACnn_n'nnGTC
Cuts at: 4874 4874
Size: 6145
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4954
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6145
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3243 3275 3243
Size: 32 6113
AlwNI CAG_nnn'CTG
Cuts at: 2542 2764 3888 5460 2542
Size: 222 1124 1572 3227
Fragments arranged by size:
3227 1572 1124 222
ApaLI G'TGCA_C
Cuts at: 4202 5358 4202
Size: 1156 4989
ApoI r'AATT_y
Cuts at: 1493 3454 3680 3832 5941 1493
Size: 1961 226 152 2109 1697
Fragments arranged by size:
2109 1961 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4699 160
Size: 872 3667 1606
Fragments arranged by size:
3667 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
AvaI C'yCGr_G
Cuts at: 1870 1943 3470 1870
Size: 73 1527 4545
Fragments arranged by size:
4545 1527 73
BamHI G'GATC_C
Cuts at: 2420 3475 3842 2420
Size: 1055 367 4723
Fragments arranged by size:
4723 1055 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2304 2703 3047 3466 4921
Size: 325 217 627 517 399 344 419 1455
Cuts at: 4921 618
Size: 1842
Fragments arranged by size:
1842 1455 627 517 419 399 344 325
217
BanII G_rGCy'C
Cuts at: 1391 2169 2499 3117 3464 1391
Size: 778 330 618 347 4072
Fragments arranged by size:
4072 778 618 347 330
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2828 928
Size: 971 929 4245
Fragments arranged by size:
4245 971 929
BbvCI CC'TCA_GC
Cuts at: 2069 2543 2069
Size: 474 5671
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4336 4370 1903
Size: 34 50 34 2315 34 3678
Fragments arranged by size:
3678 2315 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4037 5247 4037
Size: 1210 4935
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6145
BfrBI ATG'CAT
Cuts at: 3425 3425
Size: 6145
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4756 136
Size: 107 122 71 631 605 3084 1525
Fragments arranged by size:
3084 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6140 6140
Size: 6145
BlpI GC'TnA_GC
Cuts at: 3562 3562
Size: 6145
Bme1580I G_kGCm'C
Cuts at: 1790 3052 3347 4206 5362 1790
Size: 1262 295 859 1156 2573
Fragments arranged by size:
2573 1262 1156 859 295
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6145
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2628 3240 3876
Size: 416 53 83 399 24 1211 612 636
Cuts at: 3876 4834 442
Size: 958 1753
Fragments arranged by size:
1753 1211 958 636 612 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6145
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2837 4805 1239
Size: 1598 1968 2579
Fragments arranged by size:
2579 1968 1598
Bpu10I CC'TnA_GC
Cuts at: 2069 2543 2069
Size: 474 5671
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4270 5135 5433 4270
Size: 865 298 4982
Fragments arranged by size:
4982 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2275 2606 4808 882
Size: 617 776 331 2202 2219
Fragments arranged by size:
2219 2202 776 617 331
BsaAI yAC'GTr
Cuts at: 493 1204 3936 493
Size: 711 2732 2702
Fragments arranged by size:
2732 2702 711
BsaBI GATnn'nnATC
Cuts at: 2553 6139 2553
Size: 3586 2559
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3279 3952 4334 4969
Size: 53 83 186 936 1746 673 382 635
Cuts at: 4969 275
Size: 1451
Fragments arranged by size:
1746 1451 936 673 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2423 3261 4578 4976 5250 5397 5808 2423
Size: 838 1317 398 274 147 411 2760
Fragments arranged by size:
2760 1317 838 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2556 2586 1178
Size: 30 165 30 1153 30 4737
Fragments arranged by size:
4737 1153 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6145
BseYI C'CCAG_C
Cuts at: 2216 5348 5844 2216
Size: 3132 496 2517
Fragments arranged by size:
3132 2517 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2335 2537 1163
Size: 573 599 202 4771
Fragments arranged by size:
4771 599 573 202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3079 3595 4356 5384 1540
Size: 275 186 1078 516 761 1028 2301
Fragments arranged by size:
2301 1078 1028 761 516 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 2964 3117 4206 4291 5362 2499
Size: 465 153 1089 85 1071 3282
Fragments arranged by size:
3282 1089 1071 465 153 85
BsmI GAATG_Cn'
Cuts at: 3656 3749 3656
Size: 93 6052
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2169 2499 2964 3052 3117 3347 3464
Size: 399 379 330 465 88 65 230 117
Cuts at: 3464 4206 4291 5362 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 465 399 379 330 230
117 88 85 65
BspEI T'CCGG_A
Cuts at: 2423 3261 5808 2423
Size: 838 2547 2760
Fragments arranged by size:
2760 2547 838
BspHI T'CATG_A
Cuts at: 3136 4034 5764 3136
Size: 898 1730 3517
Fragments arranged by size:
3517 1730 898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3500 844
Size: 2656 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4032 1394
Size: 429 2209 3507
Fragments arranged by size:
3507 2209 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4640 4814 65
Size: 1183 3392 174 1396
Fragments arranged by size:
3392 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4789 4976 1700
Size: 110 2979 187 2869
Fragments arranged by size:
2979 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6145
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6145
BssSI C'ACGA_G
Cuts at: 4205 5217 4205
Size: 1012 5133
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2524 794
Size: 1730 4415
BstBI TT'CG_AA
Cuts at: 2436 4949 2436
Size: 2513 3632
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6145
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 2848
Size: 6145
BstYI r'GATC_y
Cuts at: 1972 2420 3475 3842 4227 4244 5696 5782 5794
Size: 448 1055 367 385 17 1452 86 12
Cuts at: 5794 6140 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1055 448 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2293 513
Size: 552 1228 4365
Fragments arranged by size:
4365 1228 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5406
Fragments arranged by size:
5406 683 56
BtsI GCAGTG_nn'
Cuts at: 3409 3661 4455 4475 3409
Size: 252 794 20 5079
Fragments arranged by size:
5079 794 252 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6145
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6110
DraI TTT'AAA
Cuts at: 3449 3449
Size: 6145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5152 809
Size: 507 3836 1802
Fragments arranged by size:
3836 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2223 2838 3076
Size: 54 1425 50 275 18 393 615 238
Cuts at: 3076 3592 4481 6110 8
Size: 516 889 1629 43
Fragments arranged by size:
1629 1425 889 615 516 393 275 238
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3076 3592 1537
Size: 275 1264 516 4090
Fragments arranged by size:
4090 1264 516 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3105 4075 1353
Size: 1752 970 3423
Fragments arranged by size:
3423 1752 970
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4732 5106 5252 387
Size: 1064 138 3143 374 146 1280
Fragments arranged by size:
3143 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2497 3115 2497
Size: 618 5527
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2837 4208 4805 5592 5860 1239
Size: 393 1205 1371 597 787 268 1524
Fragments arranged by size:
1524 1371 1205 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3454 3832 3454
Size: 378 5767
EcoRV GAT'ATC
Cuts at: 2551 2551
Size: 6145
FspI TGC'GCA
Cuts at: 4651 4651
Size: 6145
HaeII r_GCGC'y
Cuts at: 1678 5292 1678
Size: 3614 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4786 4818 4860 4892 1373
Size: 32 135 32 3214 32 42 32 2626
Fragments arranged by size:
3214 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2103 2953 3489 3735 669
Size: 988 281 165 850 536 246 3079
Fragments arranged by size:
3079 988 850 536 281 246 165
HindIII A'AGCT_T
Cuts at: 748 2394 748
Size: 1646 4499
HpaI GTT'AAC
Cuts at: 3735 3735
Size: 6145
KpnI G_GTAC'C
Cuts at: 2308 3470 2308
Size: 1162 4983
MfeI C'AATT_G
Cuts at: 3744 3744
Size: 6145
MluI A'CGCG_T
Cuts at: 6021 6021
Size: 6145
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5259 5443 1305
Size: 3954 184 2007
Fragments arranged by size:
3954 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2840 10
Size: 54 1425 343 1008 3315
Fragments arranged by size:
3315 1425 1008 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 4103 4462 4621 518
Size: 648 1191 1746 359 159 2042
Fragments arranged by size:
2042 1746 1191 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2539 2815 3561 4238 5386 5631 1606
Size: 933 276 746 677 1148 245 2120
Fragments arranged by size:
2120 1148 933 746 677 276 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5593
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6145
NotI GC'GGCC_GC
Cuts at: 3592 3592
Size: 6145
NsiI A_TGCA'T
Cuts at: 3427 3427
Size: 6145
NspI r_CATG'y
Cuts at: 1132 1229 2282 2413 3385 3429 3503 5048 1132
Size: 97 1053 131 972 44 74 1545 2229
Fragments arranged by size:
2229 1545 1053 972 131 97 74 44
PasI CC'CwG_GG
Cuts at: 1348 3394 1348
Size: 2046 4099
PciI A'CATG_T
Cuts at: 2409 3381 5044 2409
Size: 972 1663 3510
Fragments arranged by size:
3510 1663 972
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2586 2764 1108
Size: 1478 178 4489
Fragments arranged by size:
4489 1478 178
PmeI GTTT'AAAC
Cuts at: 3449 3449
Size: 6145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4214 4246 5727 5759 4214
Size: 32 1481 32 4600
Fragments arranged by size:
4600 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6145
PsiI TTA'TAA
Cuts at: 3715 3715
Size: 6145
PstI C_TGCA'G
Cuts at: 830 2079 2596 3497 830
Size: 1249 517 901 3478
Fragments arranged by size:
3478 1249 901 517
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6145
PvuII CAG'CTG
Cuts at: 2539 2815 2539
Size: 276 5869
SacI G_AGCT'C
Cuts at: 2499 3117 2499
Size: 618 5527
SalI G'TCGA_C
Cuts at: 1936 2101 3487 1936
Size: 165 1386 4594
Fragments arranged by size:
4594 1386 165
SapI GCTCTTCn'nnn_
Cuts at: 3105 3105
Size: 6145
SbfI CC_TGCA'GG
Cuts at: 3497 3497
Size: 6145
ScaI AGT'ACT
Cuts at: 4393 6105 4393
Size: 1712 4433
SfcI C'TryA_G
Cuts at: 826 1045 2075 2592 3493 4628 5309 5500 826
Size: 219 1030 517 901 1135 681 191 1471
Fragments arranged by size:
1471 1135 1030 901 681 517 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6145
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6145
SmaI CCC'GGG
Cuts at: 1872 3472 1872
Size: 1600 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4249 5150 5412 820
Size: 197 926 2306 901 262 1553
Fragments arranged by size:
2306 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3936 493
Size: 3443 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6145
SphI G_CATG'C
Cuts at: 1132 3503 1132
Size: 2371 3774
SspI AAT'ATT
Cuts at: 5 52 4069 5
Size: 47 4017 2081
Fragments arranged by size:
4017 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6145
StyI C'CwwG_G
Cuts at: 513 1065 3168 3194 3584 513
Size: 552 2103 26 390 3074
Fragments arranged by size:
3074 2103 552 390 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2397 2490 3035 3386 4172 4189 4342
Size: 1255 817 93 545 351 786 17 153
Cuts at: 4342 4527 4972 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 817 786 545 445 351 185
153 93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 3024 4391
Size: 274 80 33 51 1443 225 822 1367
Cuts at: 4391 6103 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1367 822 274 225 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3073 3105 6105 6137 3073
Size: 32 3000 32 3081
Fragments arranged by size:
3081 3000 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6145
XbaI T'CTAG_A
Cuts at: 3481 3481
Size: 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2679 2679
Size: 6145
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6145
XmaI C'CCGG_G
Cuts at: 1870 3470 1870
Size: 1600 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 4274
Size: 6145
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3953 276
Size: 53 83 186 936 2419 2468
Fragments arranged by size:
2468 2419 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI