Restriction map for
pFN21AB9509
(Circular) (Six-base) MAPSORT of: pFN21AB9509.seq Check: 9137 from: 1 to: 5785
pFN21AB9509 5785 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3595 278
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3128 1937
Size: 1191 4594
Acc65I G'GTAC_C
Cuts at: 3106 3106
Size: 5785
AclI AA'CG_TT
Cuts at: 1563 3912 4285 1563
Size: 2349 373 3063
Fragments arranged by size:
3063 2349 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2168 2315 2657 2975 3848 5232 5500
Size: 465 71 147 342 318 873 1384 268
Cuts at: 5500 1632
Size: 1917
Fragments arranged by size:
1917 1384 873 465 342 318 268 147
71
AflII C'TTAA_G
Cuts at: 820 1017 2548 820
Size: 197 1531 4057
Fragments arranged by size:
4057 1531 197
AflIII A'CryG_T
Cuts at: 1203 4684 5661 1203
Size: 3481 977 1327
Fragments arranged by size:
3481 1327 977
AgeI A'CCGG_T
Cuts at: 4616 4616
Size: 5785
AhdI GACnn_n'nnGTC
Cuts at: 4514 4514
Size: 5785
AleI CACnn'nnGTG
Cuts at: 1166 2112 1166
Size: 946 4839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3081 1184
Size: 1897 3888
AlwNI CAG_nnn'CTG
Cuts at: 2146 2680 2755 3528 5100 2146
Size: 534 75 773 1572 2831
Fragments arranged by size:
2831 1572 773 534 75
ApaLI G'TGCA_C
Cuts at: 3842 4998 3842
Size: 1156 4629
ApoI r'AATT_y
Cuts at: 1493 2326 3094 3320 3472 5581 1493
Size: 833 768 226 152 2109 1697
Fragments arranged by size:
2109 1697 833 768 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4339 160
Size: 872 3307 1606
Fragments arranged by size:
3307 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
AvaI C'yCGr_G
Cuts at: 1870 1943 3110 1870
Size: 73 1167 4545
Fragments arranged by size:
4545 1167 73
BamHI G'GATC_C
Cuts at: 3115 3482 3115
Size: 367 5418
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3106 4561 618
Size: 325 217 627 1319 1455 1842
Fragments arranged by size:
1842 1455 1319 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3104 1391
Size: 1713 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2312 2360 928
Size: 971 413 48 4353
Fragments arranged by size:
4353 971 413 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2101 2135 3976 4010 1903
Size: 34 164 34 1841 34 3678
Fragments arranged by size:
3678 1841 164 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3677 4887 3677
Size: 1210 4575
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5785
BfrBI ATG'CAT
Cuts at: 2340 2340
Size: 5785
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2790 4396
Size: 107 122 71 631 605 337 781 1606
Cuts at: 4396 136
Size: 1525
Fragments arranged by size:
1606 1525 781 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5780 5780
Size: 5785
BlpI GC'TnA_GC
Cuts at: 3202 3202
Size: 5785
Bme1580I G_kGCm'C
Cuts at: 1790 3846 5002 1790
Size: 2056 1156 2573
Fragments arranged by size:
2573 2056 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5785
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3010 3516 4474
Size: 416 53 83 399 24 1593 506 958
Cuts at: 4474 442
Size: 1753
Fragments arranged by size:
1753 1593 958 506 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5785
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2372 2438 2456 2654 2834 2956 2993 3008
Size: 1133 66 18 198 180 122 37 15
Cuts at: 3008 4445 1239
Size: 1437 2579
Fragments arranged by size:
2579 1437 1133 198 180 122 66 37
18 15
Bpu10I CC'TnA_GC
Cuts at: 2780 2780
Size: 5785
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2948 3910 4775 5073 2948
Size: 962 865 298 3660
Fragments arranged by size:
3660 962 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4448 882
Size: 617 2949 2219
Fragments arranged by size:
2949 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3576 493
Size: 711 2372 2702
Fragments arranged by size:
2702 2372 711
BsaBI GATnn'nnATC
Cuts at: 2253 5779 2253
Size: 3526 2259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3592 3974 4609 275
Size: 53 83 186 936 2059 382 635 1451
Fragments arranged by size:
2059 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4218 4616 4890 5037 5448 4218
Size: 398 274 147 411 4555
Fragments arranged by size:
4555 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2627 2657 2966 2996 1178
Size: 30 165 30 1224 30 309 30 3967
Fragments arranged by size:
3967 1224 309 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2186 2369 1186
Size: 988 12 183 4602
Fragments arranged by size:
4602 988 183 12
BseYI C'CCAG_C
Cuts at: 2581 4988 5484 2581
Size: 2407 496 2882
Fragments arranged by size:
2882 2407 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5212
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3235 3996 5024 1540
Size: 275 186 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3846 3931 5002 3846
Size: 85 1071 4629
Fragments arranged by size:
4629 1071 85
BsmI GAATG_Cn'
Cuts at: 3296 3389 3296
Size: 93 5692
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3104 3846 3931 5002 1391
Size: 399 1314 742 85 1071 2174
Fragments arranged by size:
2174 1314 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5448 5448
Size: 5785
BspHI T'CATG_A
Cuts at: 3674 5404 3674
Size: 1730 4055
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2772 3140 844
Size: 1928 368 3489
Fragments arranged by size:
3489 1928 368
BsrBI CCG'CTC
Cuts at: 1394 1823 2164 2992 3672 1394
Size: 429 341 828 680 3507
Fragments arranged by size:
3507 828 680 429 341
BsrDI GCAATG_nn'
Cuts at: 65 1248 2844 4280 4454 65
Size: 1183 1596 1436 174 1396
Fragments arranged by size:
1596 1436 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2724 3021 4429 4616 1700
Size: 110 914 297 1408 187 2869
Fragments arranged by size:
2869 1408 914 297 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5785
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5785
BssSI C'ACGA_G
Cuts at: 3845 4857 3845
Size: 1012 4773
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5785
BstBI TT'CG_AA
Cuts at: 4589 4589
Size: 5785
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5785
BstXI CCAn_nnnn'nTGG
Cuts at: 2224 2971 2224
Size: 747 5038
BstYI r'GATC_y
Cuts at: 1972 2380 2585 3115 3482 3867 3884 5336 5422
Size: 408 205 530 367 385 17 1452 86
Cuts at: 5422 5434 5780 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 530 408 385 367 346 205
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2103 487
Size: 683 56 877 4169
Fragments arranged by size:
4169 877 683 56
BtsI GCAGTG_nn'
Cuts at: 2119 3301 4095 4115 2119
Size: 1182 794 20 3789
Fragments arranged by size:
3789 1182 794 20
ClaI AT'CG_AT
Cuts at: 1554 3063 1554
Size: 1509 4276
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5750
DraI TTT'AAA
Cuts at: 3089 3089
Size: 5785
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2066 4792 809
Size: 507 750 2726 1802
Fragments arranged by size:
2726 1802 750 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 3232 4121
Size: 54 1425 50 275 18 294 1108 889
Cuts at: 4121 5750 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1108 889 294 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3232 1537
Size: 275 1420 4090
Fragments arranged by size:
4090 1420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2516 3715 1353
Size: 1163 1199 3423
Fragments arranged by size:
3423 1199 1163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 2309 3040 4372 4746 4892
Size: 1064 138 438 282 731 1332 374 146
Cuts at: 4892 387
Size: 1280
Fragments arranged by size:
1332 1280 1064 731 438 374 282 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2168 2315 2372 2438 2456 2654
Size: 393 465 71 147 57 66 18 198
Cuts at: 2654 2657 2834 2956 2975 2993 3008 3848 4445
Size: 3 177 122 19 18 15 840 597
Cuts at: 4445 5232 5500 1239
Size: 787 268 1524
Fragments arranged by size:
1524 840 787 597 465 393 268 198
177 147 122 71 66 57 19 18
18 15 3
EcoO109I rG'GnC_Cy
Cuts at: 2176 2809 3010 2176
Size: 633 201 4951
Fragments arranged by size:
4951 633 201
EcoRI G'AATT_C
Cuts at: 3094 3472 3094
Size: 378 5407
FspI TGC'GCA
Cuts at: 4291 4291
Size: 5785
HaeII r_GCGC'y
Cuts at: 1678 2426 4932 1678
Size: 748 2506 2531
Fragments arranged by size:
2531 2506 748
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2398 2430 2570 2602 2728
Size: 32 135 32 826 32 140 32 126
Cuts at: 2728 2760 2834 2866 4426 4458 4500 4532 1373
Size: 32 74 32 1560 32 42 32 2626
Fragments arranged by size:
2626 1560 826 140 135 126 74 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2059 2485 2493 2521 3129 3375
Size: 988 281 121 426 8 28 608 246
Cuts at: 3375 669
Size: 3079
Fragments arranged by size:
3079 988 608 426 281 246 121 28
8
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5785
HpaI GTT'AAC
Cuts at: 3375 3375
Size: 5785
KpnI G_GTAC'C
Cuts at: 3110 3110
Size: 5785
MfeI C'AATT_G
Cuts at: 3384 3384
Size: 5785
MluI A'CGCG_T
Cuts at: 5661 5661
Size: 5785
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 4899 5083 1305
Size: 1515 2079 184 2007
Fragments arranged by size:
2079 2007 1515 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3963
Fragments arranged by size:
3963 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2112 3743 4102 4261 518
Size: 648 946 1631 359 159 2042
Fragments arranged by size:
2042 1631 946 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3201 3878 5026 5271 1606
Size: 1595 677 1148 245 2120
Fragments arranged by size:
2120 1595 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4294
Fragments arranged by size:
4294 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5785
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5785
NotI GC'GGCC_GC
Cuts at: 3232 3232
Size: 5785
NsiI A_TGCA'T
Cuts at: 2342 2342
Size: 5785
NspI r_CATG'y
Cuts at: 1132 1229 3143 4688 1132
Size: 97 1914 1545 2229
Fragments arranged by size:
2229 1914 1545 97
PacI TTA_AT'TAA
Cuts at: 2334 2334
Size: 5785
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5785
PciI A'CATG_T
Cuts at: 4684 4684
Size: 5785
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5785
PmeI GTTT'AAAC
Cuts at: 3089 3089
Size: 5785
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3854 3886 5367 5399 3854
Size: 32 1481 32 4240
Fragments arranged by size:
4240 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2176 3010 2176
Size: 834 4951
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5785
PsiI TTA'TAA
Cuts at: 3355 3355
Size: 5785
PstI C_TGCA'G
Cuts at: 830 2751 3137 830
Size: 1921 386 3478
Fragments arranged by size:
3478 1921 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5785
SalI G'TCGA_C
Cuts at: 1936 3127 1936
Size: 1191 4594
SbfI CC_TGCA'GG
Cuts at: 3137 3137
Size: 5785
ScaI AGT'ACT
Cuts at: 4033 5745 4033
Size: 1712 4073
SexAI A'CCwGG_T
Cuts at: 2754 2754
Size: 5785
SfcI C'TryA_G
Cuts at: 826 1045 2360 2567 2747 3133 4268 4949 5140
Size: 219 1315 207 180 386 1135 681 191
Cuts at: 5140 826
Size: 1471
Fragments arranged by size:
1471 1315 1135 681 386 219 207 191
180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5785
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5785
SmaI CCC'GGG
Cuts at: 1872 3112 1872
Size: 1240 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2548 2927 3889 4790 5052 820
Size: 197 926 605 379 962 901 262 1553
Fragments arranged by size:
1553 962 926 901 605 379 262 197
SnaBI TAC'GTA
Cuts at: 493 3576 493
Size: 3083 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5785
SphI G_CATG'C
Cuts at: 1132 3143 1132
Size: 2011 3774
SspI AAT'ATT
Cuts at: 5 52 3709 5
Size: 47 3657 2081
Fragments arranged by size:
3657 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 3224 513
Size: 552 939 1220 3074
Fragments arranged by size:
3074 1220 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3812 3829 3982 4167 4612 325
Size: 1255 2232 17 153 185 445 1498
Fragments arranged by size:
2232 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4031 5743 96
Size: 274 80 33 51 1443 2054 1712 138
Fragments arranged by size:
2054 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5745 5777 5745
Size: 32 5753
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5785
XbaI T'CTAG_A
Cuts at: 2443 3121 2443
Size: 678 5107
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5785
XmaI C'CCGG_G
Cuts at: 1870 3110 1870
Size: 1240 4545
XmnI GAAnn'nnTTC
Cuts at: 3914 3914
Size: 5785
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3593 276
Size: 53 83 186 936 2059 2468
Fragments arranged by size:
2468 2059 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI