Restriction map for
pFN21AB9506
(Circular) (Six-base) MAPSORT of: pFN21AB9506.seq Check: 1770 from: 1 to: 6031
pFN21AB9506 6031 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2344 2344
Size: 6031
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3374 1937
Size: 1437 4594
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2284 4094 5478 5746 1632
Size: 652 1810 1384 268 1917
Fragments arranged by size:
1917 1810 1384 652 268
AfeI AGC'GCT
Cuts at: 2150 2150
Size: 6031
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 4930 5907 1203
Size: 3727 977 1327
Fragments arranged by size:
3727 1327 977
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 4333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6031
AlwNI CAG_nnn'CTG
Cuts at: 2543 3774 5346 2543
Size: 1231 1572 3228
Fragments arranged by size:
3228 1572 1231
ApaI G_GGCC'C
Cuts at: 2079 2086 2146 2943 3077 2079
Size: 7 60 797 134 5033
Fragments arranged by size:
5033 797 134 60 7
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 3340 3566 3718 5827 1493
Size: 1847 226 152 2109 1697
Fragments arranged by size:
2109 1847 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2131 2131
Size: 6031
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2242 2254 2684 3070 3103 3356
Size: 73 135 164 12 430 386 33 253
Cuts at: 3356 1870
Size: 4545
Fragments arranged by size:
4545 430 386 253 164 135 73 33
12
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2689 3062 3191 3352
Size: 325 217 627 264 638 373 129 161
Cuts at: 3352 4807 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 638 627 373 325 264 217
161 129
BanII G_rGCy'C
Cuts at: 1391 2079 2086 2146 2195 2255 2391 2460 2779
Size: 688 7 60 49 60 136 69 319
Cuts at: 2779 2943 3077 3350 1391
Size: 164 134 273 4072
Fragments arranged by size:
4072 688 319 273 164 136 134 69
60 60 49 7
BbeI G_GCGC'C
Cuts at: 2055 2693 3195 2055
Size: 638 502 4891
Fragments arranged by size:
4891 638 502
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5060
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4222 4256 1903
Size: 34 2285 34 3678
Fragments arranged by size:
3678 2285 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3149 3923 5133 3149
Size: 774 1210 4047
Fragments arranged by size:
4047 1210 774
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2045 2141 2552
Size: 107 122 71 631 605 373 96 411
Cuts at: 2552 4642 136
Size: 2090 1525
Fragments arranged by size:
2090 1525 631 605 411 373 122 107
96 71
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 3112 3120 3448 3112
Size: 8 328 5695
Fragments arranged by size:
5695 328 8
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2086 2146 2375 2943 3067 3077 4092
Size: 289 7 60 229 568 124 10 1015
Cuts at: 4092 5248 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1015 568 289 229 124 60
10 7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2937 3762 4720
Size: 416 53 83 399 24 1520 825 958
Cuts at: 4720 442
Size: 1753
Fragments arranged by size:
1753 1520 958 825 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2410 2710 2410
Size: 300 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2432 2868 3164 3287 4691 1239
Size: 968 225 436 296 123 1404 2579
Fragments arranged by size:
2579 1404 968 436 296 225 123
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4156 5021 5319 4156
Size: 865 298 4868
Fragments arranged by size:
4868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2167 2882 2966 4694 882
Size: 617 668 715 84 1728 2219
Fragments arranged by size:
2219 1728 715 668 617 84
BsaAI yAC'GTr
Cuts at: 493 1204 3822 493
Size: 711 2618 2702
Fragments arranged by size:
2702 2618 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2690 3192 3838
Size: 53 83 186 936 519 638 502 646
Cuts at: 3838 4220 4855 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 646 638 635 519 502 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2926 4464 4862 5136 5283 5694 2926
Size: 1538 398 274 147 411 3263
Fragments arranged by size:
3263 1538 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2866 2896 1178
Size: 30 165 30 1463 30 4313
Fragments arranged by size:
4313 1463 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2836 3332 1186
Size: 1650 496 3885
Fragments arranged by size:
3885 1650 496
BseYI C'CCAG_C
Cuts at: 2145 2459 2567 5234 5730 2145
Size: 314 108 2667 496 2446
Fragments arranged by size:
2667 2446 496 314 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5458
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 3481 4242 5270 1540
Size: 275 186 72 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 2391 2828 3162 4092 4177 5248 2391
Size: 437 334 930 85 1071 3174
Fragments arranged by size:
3174 1071 930 437 334 85
BsiWI C'GTAC_G
Cuts at: 2626 2626
Size: 6031
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 2485 2485
Size: 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2086 2146 2195 2255 2375 2391
Size: 399 289 7 60 49 60 120 16
Cuts at: 2391 2460 2779 2828 2943 3067 3077 3162 3350
Size: 69 319 49 115 124 10 85 188
Cuts at: 3350 4092 4177 5248 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 289 188 124
120 115 85 85 69 60 60 49
49 16 10 7
BspEI T'CCGG_A
Cuts at: 5694 5694
Size: 6031
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2344 3386 844
Size: 1500 1042 3489
Fragments arranged by size:
3489 1500 1042
BsrBI CCG'CTC
Cuts at: 1394 1823 3148 3918 1394
Size: 429 1325 770 3507
Fragments arranged by size:
3507 1325 770 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4526 4700 65
Size: 1183 3278 174 1396
Fragments arranged by size:
3278 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 2788 4675 4862 1700
Size: 110 238 740 1887 187 2869
Fragments arranged by size:
2869 1887 740 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 2131 1925
Size: 206 5825
BssSI C'ACGA_G
Cuts at: 4091 5103 4091
Size: 1012 5019
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6031
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstXI CCAn_nnnn'nTGG
Cuts at: 2431 2491 2866 3001 2431
Size: 60 375 135 5461
Fragments arranged by size:
5461 375 135 60
BstYI r'GATC_y
Cuts at: 1972 3361 3728 4113 4130 5582 5668 5680 6026
Size: 1389 367 385 17 1452 86 12 346
Cuts at: 6026 1972
Size: 1977
Fragments arranged by size:
1977 1452 1389 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2041 2067 2086 2136 2178 2490 2706
Size: 552 976 26 19 50 42 312 216
Cuts at: 2706 513
Size: 3838
Fragments arranged by size:
3838 976 552 312 216 50 42 26
19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5292
Fragments arranged by size:
5292 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 2499 2601 2844 3050 3218 3547 4341 4361
Size: 335 102 243 206 168 329 794 20
Cuts at: 4361 2164
Size: 3834
Fragments arranged by size:
3834 794 335 329 243 206 168 102
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2532 2553
Size: 54 1425 50 275 18 240 462 21
Cuts at: 2553 2595 2768 2790 3024 3478 4367 5996 8
Size: 42 173 22 234 454 889 1629 43
Fragments arranged by size:
1629 1425 889 462 454 275 240 234
173 54 50 43 42 22 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2070 3478 1537
Size: 275 258 1408 4090
Fragments arranged by size:
4090 1408 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2448 2964 3128 3961 1353
Size: 1026 69 516 164 833 3423
Fragments arranged by size:
3423 1026 833 516 164 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4618 4992 5138 387
Size: 1064 138 3029 374 146 1280
Fragments arranged by size:
3029 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2389 2389
Size: 6031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2284 2432 2868 3164 3287 4094
Size: 393 575 77 148 436 296 123 807
Cuts at: 4094 4691 5478 5746 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 807 787 597 575 436 393 296
268 148 123 77
EcoO109I rG'GnC_Cy
Cuts at: 2082 2142 2521 2731 2939 3074 2082
Size: 60 379 210 208 135 5039
Fragments arranged by size:
5039 379 210 208 135 60
EcoRI G'AATT_C
Cuts at: 3340 3718 3340
Size: 378 5653
FseI GG_CCGG'CC
Cuts at: 2792 2792
Size: 6031
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 2055 2152 2404 2693 3195 5178 1678
Size: 377 97 252 289 502 1983 2531
Fragments arranged by size:
2531 1983 502 377 289 252 97
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2225 2257 2258
Size: 32 135 32 620 32 1 32 1
Cuts at: 2258 2290 2866 2898 4672 4704 4746 4778 1373
Size: 32 576 32 1774 32 42 32 2626
Fragments arranged by size:
2626 1774 620 576 135 42 32 32
32 32 32 32 32 32 1 1
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6031
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KasI G'GCGC_C
Cuts at: 2051 2689 3191 2051
Size: 638 502 4891
Fragments arranged by size:
4891 638 502
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 5907 5907
Size: 6031
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5145 5329 1305
Size: 3840 184 2007
Fragments arranged by size:
3840 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2534 2555 10
Size: 54 1425 343 702 21 3486
Fragments arranged by size:
3486 1425 702 343 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 2864 3989 4348 4507 518
Size: 648 1323 375 1125 359 159 2042
Fragments arranged by size:
2042 1323 1125 648 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2069 2088 2107 2138 3116 3124 3447 4124
Size: 463 19 19 31 978 8 323 677
Cuts at: 4124 5272 5517 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 978 677 463 323 245 31
19 19 8
NaeI GCC'GGC
Cuts at: 2050 2790 2050
Size: 740 5291
NarI GG'CG_CC
Cuts at: 2052 2690 3192 2052
Size: 638 502 4891
Fragments arranged by size:
4891 638 502
NcoI C'CATG_G
Cuts at: 513 1065 2490 513
Size: 552 1425 4054
Fragments arranged by size:
4054 1425 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NgoMIV G'CCGG_C
Cuts at: 2048 2788 2048
Size: 740 5291
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 3389 4934 1132
Size: 97 2160 1545 2229
Fragments arranged by size:
2229 2160 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2836 1348
Size: 1488 4543
PciI A'CATG_T
Cuts at: 4930 4930
Size: 6031
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2368 1108
Size: 1260 4771
PfoI T'CCnGG_A
Cuts at: 2448 2907 3084 3141 2448
Size: 459 177 57 5338
Fragments arranged by size:
5338 459 177 57
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2521 2521
Size: 6031
PshAI GACnn'nnGTC
Cuts at: 1613 2444 1613
Size: 831 5200
PsiI TTA'TAA
Cuts at: 2612 3601 2612
Size: 989 5042
PspOMI G'GGCC_C
Cuts at: 2075 2082 2142 2939 3073 2075
Size: 7 60 797 134 5033
Fragments arranged by size:
5033 797 134 60 7
PstI C_TGCA'G
Cuts at: 830 2581 3383 830
Size: 1751 802 3478
Fragments arranged by size:
3478 1751 802
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
PvuII CAG'CTG
Cuts at: 3116 3124 3116
Size: 8 6023
SacI G_AGCT'C
Cuts at: 2391 2391
Size: 6031
SacII CC_GC'GG
Cuts at: 2070 2089 2139 2070
Size: 19 50 5962
Fragments arranged by size:
5962 50 19
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SanDI GG'GwC_CC
Cuts at: 2521 2521
Size: 6031
SapI GCTCTTCn'nnn_
Cuts at: 2379 2448 2379
Size: 69 5962
SbfI CC_TGCA'GG
Cuts at: 3383 3383
Size: 6031
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 2577 3379 4514 5195 5386 826
Size: 219 1532 802 1135 681 191 1471
Fragments arranged by size:
1532 1471 1135 802 681 219 191
SfoI GGC'GCC
Cuts at: 2053 2691 3193 2053
Size: 638 502 4891
Fragments arranged by size:
4891 638 502
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 2080 3072 3358 1872
Size: 208 992 286 4545
Fragments arranged by size:
4545 992 286 208
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4135 5036 5298 820
Size: 197 926 2192 901 262 1553
Fragments arranged by size:
2192 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3822 493
Size: 3329 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SrfI GCCC'GGGC
Cuts at: 3072 3072
Size: 6031
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6031
StyI C'CwwG_G
Cuts at: 513 1065 2490 3027 3057 3470 513
Size: 552 1425 537 30 413 3074
Fragments arranged by size:
3074 1425 552 537 413 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2755 2813 4058 4075 4228 4413 4858
Size: 1255 1175 58 1245 17 153 185 445
Cuts at: 4858 325
Size: 1498
Fragments arranged by size:
1498 1255 1245 1175 445 185 153 58
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4277 5989 96
Size: 274 80 33 51 1443 2300 1712 138
Fragments arranged by size:
2300 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5991 6023 5991
Size: 32 5999
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2428 2431 2428
Size: 3 6028
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 2078 3070 3356 1870
Size: 208 992 286 4545
Fragments arranged by size:
4545 992 286 208
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI Bpu10I BstZ17I Bsu36I
DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SexAI SfiI SwaI