Restriction map for
pFN21AB9504
(Circular) (Six-base) MAPSORT of: pFN21AB9504.seq Check: 9985 from: 1 to: 8647
pFN21AB9504 8647 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3846 5231 3846
Size: 1385 7262
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6457 278
Size: 53 83 186 936 4921 2468
Fragments arranged by size:
4921 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4491 5990 1937
Size: 2554 1499 4594
Fragments arranged by size:
4594 2554 1499
Acc65I G'GTAC_C
Cuts at: 4944 5968 4944
Size: 1024 7623
AclI AA'CG_TT
Cuts at: 1563 6774 7147 1563
Size: 5211 373 3063
Fragments arranged by size:
5211 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3548 3730 6710 8094 8362 1632
Size: 1916 182 2980 1384 268 1917
Fragments arranged by size:
2980 1917 1916 1384 268 182
AfeI AGC'GCT
Cuts at: 5630 5630
Size: 8647
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8450
AflIII A'CryG_T
Cuts at: 1203 4791 7546 8523 1203
Size: 3588 2755 977 1327
Fragments arranged by size:
3588 2755 1327 977
AgeI A'CCGG_T
Cuts at: 7478 7478
Size: 8647
AhdI GACnn_n'nnGTC
Cuts at: 7376 7376
Size: 8647
AleI CACnn'nnGTG
Cuts at: 1166 2390 5276 5522 5888 1166
Size: 1224 2886 246 366 3925
Fragments arranged by size:
3925 2886 1224 366 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2552 3281 3623 5489 5557 5624 5664 1184
Size: 1368 729 342 1866 68 67 40 4167
Fragments arranged by size:
4167 1866 1368 729 342 68 67 40
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3501 3533 3501
Size: 32 8615
AlwNI CAG_nnn'CTG
Cuts at: 2066 2194 3088 3526 3817 3979 4070 4176 4300
Size: 128 894 438 291 162 91 106 124
Cuts at: 4300 4780 5359 5482 5597 5618 5695 5732 5848
Size: 480 579 123 115 21 77 37 116
Cuts at: 5848 6390 7962 2066
Size: 542 1572 2751
Fragments arranged by size:
2751 1572 894 579 542 480 438 291
162 128 124 123 116 115 106 91
77 37 21
ApaI G_GGCC'C
Cuts at: 4897 4897
Size: 8647
ApaLI G'TGCA_C
Cuts at: 2458 3077 4655 6704 7860 2458
Size: 619 1578 2049 1156 3245
Fragments arranged by size:
3245 2049 1578 1156 619
ApoI r'AATT_y
Cuts at: 1493 5132 5956 6182 6334 8443 1493
Size: 3639 824 226 152 2109 1697
Fragments arranged by size:
3639 2109 1697 824 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7201 160
Size: 872 6169 1606
Fragments arranged by size:
6169 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8647
AvaI C'yCGr_G
Cuts at: 1870 1943 4208 4592 4832 5972 1870
Size: 73 2265 384 240 1140 4545
Fragments arranged by size:
4545 2265 1140 384 240 73
BamHI G'GATC_C
Cuts at: 4908 5977 6344 4908
Size: 1069 367 7211
Fragments arranged by size:
7211 1069 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2369 4380 4870 4944 5080
Size: 325 217 627 582 2011 490 74 136
Cuts at: 5080 5278 5968 7423 618
Size: 198 690 1455 1842
Fragments arranged by size:
2011 1842 1455 690 627 582 490 325
217 198 136 74
BanII G_rGCy'C
Cuts at: 1391 2442 2912 3326 3360 3903 4674 4897 5589
Size: 1051 470 414 34 543 771 223 692
Cuts at: 5589 5966 1391
Size: 377 4072
Fragments arranged by size:
4072 1051 771 692 543 470 414 377
223 34
BbeI G_GCGC'C
Cuts at: 4874 4874
Size: 8647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3298 3769 3851 928
Size: 971 1399 471 82 5724
Fragments arranged by size:
5724 1399 971 471 82
BbvCI CC'TCA_GC
Cuts at: 4058 4283 5938 4058
Size: 225 1655 6767
Fragments arranged by size:
6767 1655 225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4544 4578 6838 6872 1903
Size: 34 2607 34 2260 34 3678
Fragments arranged by size:
3678 2607 2260 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6539 7749 6539
Size: 1210 7437
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3822 4018 7258
Size: 107 122 71 631 605 2150 196 3240
Cuts at: 7258 136
Size: 1525
Fragments arranged by size:
3240 2150 1525 631 605 196 122 107
71
BglII A'GATC_T
Cuts at: 8642 8642
Size: 8647
BlpI GC'TnA_GC
Cuts at: 3269 4133 5169 6064 3269
Size: 864 1036 895 5852
Fragments arranged by size:
5852 1036 895 864
Bme1580I G_kGCm'C
Cuts at: 1790 2462 2663 2830 3053 3081 4385 4659 4751
Size: 672 201 167 223 28 1304 274 92
Cuts at: 4751 4897 6708 7864 1790
Size: 146 1811 1156 2573
Fragments arranged by size:
2573 1811 1304 1156 672 274 223 201
167 146 92 28
BmgBI CAC'GTC
Cuts at: 1318 3289 1318
Size: 1971 6676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2666 2768 5128
Size: 416 53 83 399 24 1249 102 2360
Cuts at: 5128 5914 6378 7336 442
Size: 786 464 958 1753
Fragments arranged by size:
2360 1753 1249 958 786 464 416 399
102 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8647
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3373 3887 3917 4945 4994 5198 7307 1239
Size: 2134 514 30 1028 49 204 2109 2579
Fragments arranged by size:
2579 2134 2109 1028 514 204 49 30
Bpu10I CC'TnA_GC
Cuts at: 3482 4058 4218 4283 4457 4604 5938 3482
Size: 576 160 65 174 147 1334 6191
Fragments arranged by size:
6191 1334 576 174 160 147 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3122 4619 5006 5192 6772 7637 7935 3122
Size: 1497 387 186 1580 865 298 3834
Fragments arranged by size:
3834 1580 1497 865 387 298 186
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3422 4578 4682 7310 882
Size: 617 1923 1156 104 2628 2219
Fragments arranged by size:
2628 2219 1923 1156 617 104
BsaAI yAC'GTr
Cuts at: 493 1204 4792 6438 493
Size: 711 3588 1646 2702
Fragments arranged by size:
3588 2702 1646 711
BsaBI GATnn'nnATC
Cuts at: 5267 8641 5267
Size: 3374 5273
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4871 4992 5332 6454
Size: 53 83 186 936 3338 121 340 1122
Cuts at: 6454 6836 7471 275
Size: 382 635 1451
Fragments arranged by size:
3338 1451 1122 936 635 382 340 186
121 83 53
BsaWI w'CCGG_w
Cuts at: 5622 7080 7478 7752 7899 8310 5622
Size: 1458 398 274 147 411 5959
Fragments arranged by size:
5959 1458 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3885 3890 3915 3920 4790
Size: 30 165 30 2482 5 25 5 870
Cuts at: 4790 4820 4918 4948 1178
Size: 30 98 30 4877
Fragments arranged by size:
4877 2482 870 165 98 30 30 30
30 25 5 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 2252 2419 2501 2936 3353 3440 3452
Size: 1063 3 167 82 435 417 87 12
Cuts at: 3452 3767 3818 3953 4226 4405 4682 4850 5768
Size: 315 51 135 273 179 277 168 918
Cuts at: 5768 5926 1186
Size: 158 3907
Fragments arranged by size:
3907 1063 918 435 417 315 277 273
179 168 167 158 135 87 82 51
12 3
BseYI C'CCAG_C
Cuts at: 2155 2341 2707 2839 3031 3949 3960 4195 4300
Size: 186 366 132 192 918 11 235 105
Cuts at: 4300 5003 5084 5494 5590 7850 8346 2155
Size: 703 81 410 96 2260 496 2456
Fragments arranged by size:
2456 2260 918 703 496 410 366 235
192 186 132 105 96 81 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 5495 1163
Size: 573 378 3381 4315
Fragments arranged by size:
4315 3381 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6097 6858 7886 1540
Size: 275 186 4096 761 1028 2301
Fragments arranged by size:
4096 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2462 2912 3018 3081 3294 3360 3413 3903 4106
Size: 450 106 63 213 66 53 490 203
Cuts at: 4106 4557 4633 4659 4674 5529 6708 6793 7864
Size: 451 76 26 15 855 1179 85 1071
Cuts at: 7864 2462
Size: 3245
Fragments arranged by size:
3245 1179 1071 855 490 451 450 213
203 106 85 76 66 63 53 26
15
BsmI GAATG_Cn'
Cuts at: 6158 6251 6158
Size: 93 8554
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2442 2462 2663 2830 2912 3018 3053
Size: 399 652 20 201 167 82 106 35
Cuts at: 3053 3081 3294 3326 3360 3413 3903 4106 4385
Size: 28 213 32 34 53 490 203 279
Cuts at: 4385 4557 4633 4659 4674 4751 4897 5529 5589
Size: 172 76 26 15 77 146 632 60
Cuts at: 5589 5966 6708 6793 7864 1391
Size: 377 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 652 632 490 399 377
279 213 203 201 172 167 146 106
85 82 77 76 60 53 35 34
32 28 26 20 15
BspEI T'CCGG_A
Cuts at: 8310 8310
Size: 8647
BspHI T'CATG_A
Cuts at: 3018 4080 4269 6536 8266 3018
Size: 1062 189 2267 1730 3399
Fragments arranged by size:
3399 2267 1730 1062 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2202 2346 3225 3816 3846 5231 6002 844
Size: 1358 144 879 591 30 1385 771 3489
Fragments arranged by size:
3489 1385 1358 879 771 591 144 30
BsrBI CCG'CTC
Cuts at: 1394 1823 2379 3297 3442 5182 6534 1394
Size: 429 556 918 145 1740 1352 3507
Fragments arranged by size:
3507 1740 1352 918 556 429 145
BsrDI GCAATG_nn'
Cuts at: 65 1248 3280 7142 7316 65
Size: 1183 2032 3862 174 1396
Fragments arranged by size:
3862 2032 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2021 2787 3344 3681 4377 5199 7291
Size: 110 211 766 557 337 696 822 2092
Cuts at: 7291 7478 1700
Size: 187 2869
Fragments arranged by size:
2869 2092 822 766 696 557 337 211
187 110
BsrGI T'GTAC_A
Cuts at: 96 2394 4275 96
Size: 2298 1881 4468
Fragments arranged by size:
4468 2298 1881
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8647
BssSI C'ACGA_G
Cuts at: 6707 7719 6707
Size: 1012 7635
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5732 794
Size: 4938 3709
BstBI TT'CG_AA
Cuts at: 7451 7451
Size: 8647
BstEII G'GTnAC_C
Cuts at: 1184 5401 1184
Size: 4217 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 2689 5452 5810 2689
Size: 2763 358 5526
Fragments arranged by size:
5526 2763 358
BstYI r'GATC_y
Cuts at: 1972 3207 3522 4908 5154 5536 5977 6344 6729
Size: 1235 315 1386 246 382 441 367 385
Cuts at: 6729 6746 8198 8284 8296 8642 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1386 1235 441 385 382 367
346 315 246 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3239 3239
Size: 8647
BtgI C'CryG_G
Cuts at: 513 1065 2004 2736 3072 3915 4088 4851 513
Size: 552 939 732 336 843 173 763 4309
Fragments arranged by size:
4309 939 843 763 732 552 336 173
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7908
Fragments arranged by size:
7908 683 56
BtsI GCAGTG_nn'
Cuts at: 2661 2862 3382 3995 5463 5601 6163 6957 6977
Size: 201 520 613 1468 138 562 794 20
Cuts at: 6977 2661
Size: 4331
Fragments arranged by size:
4331 1468 794 613 562 520 201 138
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8647
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5209 5244 529
Size: 35 4645 35 3932
Fragments arranged by size:
4645 3932 35 35
DraI TTT'AAA
Cuts at: 5951 5951
Size: 8647
DraIII CAC_nnn'GTG
Cuts at: 2810 3375 2810
Size: 565 8082
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5237 7654 809
Size: 507 3921 2417 1802
Fragments arranged by size:
3921 2417 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3625 4611 4782
Size: 54 1425 50 275 18 1795 986 171
Cuts at: 4782 5812 6094 6983 8612 8
Size: 1030 282 889 1629 43
Fragments arranged by size:
1795 1629 1425 1030 986 889 282 275
171 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6094 1537
Size: 275 4282 4090
Fragments arranged by size:
4282 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2143 3110 3287 4680 6577 1353
Size: 790 967 177 1393 1897 3423
Fragments arranged by size:
3423 1897 1393 967 790 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4364 5066 7234 7608 7754 387
Size: 1064 138 2775 702 2168 374 146 1280
Fragments arranged by size:
2775 2168 1280 1064 702 374 146 138
EcoICRI GAG'CTC
Cuts at: 2910 3358 3901 4672 2910
Size: 448 543 771 6885
Fragments arranged by size:
6885 771 543 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3373 3548 3730 3887 3917 4945 4994
Size: 393 1741 175 182 157 30 1028 49
Cuts at: 4994 5198 6710 7307 8094 8362 1239
Size: 204 1512 597 787 268 1524
Fragments arranged by size:
1741 1524 1512 1028 787 597 393 268
204 182 175 157 49 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3929 5063 3929
Size: 1134 7513
EcoO109I rG'GnC_Cy
Cuts at: 2419 2686 2800 3085 3823 3976 4149 4777 5069
Size: 267 114 285 738 153 173 628 292
Cuts at: 5069 5758 2419
Size: 689 5308
Fragments arranged by size:
5308 738 689 628 292 285 267 173
153 114
EcoRI G'AATT_C
Cuts at: 5132 5956 6334 5132
Size: 824 378 7445
Fragments arranged by size:
7445 824 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2285 4660 5884 2285
Size: 2375 1224 5048
Fragments arranged by size:
5048 2375 1224
FseI GG_CCGG'CC
Cuts at: 3348 3348
Size: 8647
FspI TGC'GCA
Cuts at: 2540 2990 7153 2540
Size: 450 4163 4034
Fragments arranged by size:
4163 4034 450
HaeII r_GCGC'y
Cuts at: 1678 3817 3967 4874 5176 5632 7794 1678
Size: 2139 150 907 302 456 2162 2531
Fragments arranged by size:
2531 2162 2139 907 456 302 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2663 2695 3139 3171 3890
Size: 32 135 32 1091 32 444 32 719
Cuts at: 3890 3922 4450 4482 7288 7320 7362 7394 1373
Size: 32 528 32 2806 32 42 32 2626
Fragments arranged by size:
2806 2626 1091 719 528 444 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3909 4416 5991 6237 669
Size: 988 281 1971 507 1575 246 3079
Fragments arranged by size:
3079 1971 1575 988 507 281 246
HindIII A'AGCT_T
Cuts at: 748 3567 748
Size: 2819 5828
HpaI GTT'AAC
Cuts at: 6237 6237
Size: 8647
KasI G'GCGC_C
Cuts at: 4870 4870
Size: 8647
KpnI G_GTAC'C
Cuts at: 4948 5972 4948
Size: 1024 7623
MfeI C'AATT_G
Cuts at: 6246 6246
Size: 8647
MluI A'CGCG_T
Cuts at: 8523 8523
Size: 8647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2476 2877 4055 7761 7945 1305
Size: 1171 401 1178 3706 184 2007
Fragments arranged by size:
3706 2007 1178 1171 401 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4613 4784 5814 10
Size: 54 1425 343 2781 171 1030 2843
Fragments arranged by size:
2843 2781 1425 1030 343 171 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2390 3416 3497 3641 4179 5141 5276
Size: 648 1224 1026 81 144 538 962 135
Cuts at: 5276 5450 5471 5522 5888 6605 6964 7123 518
Size: 174 21 51 366 717 359 159 2042
Fragments arranged by size:
2042 1224 1026 962 717 648 538 366
359 174 159 144 135 81 51 21
MspA1I CmG'CkG
Cuts at: 1606 2469 2585 3478 4067 4300 4885 5344 5375
Size: 863 116 893 589 233 585 459 31
Cuts at: 5375 5732 5942 6063 6740 7888 8133 1606
Size: 357 210 121 677 1148 245 2120
Fragments arranged by size:
2120 1148 893 863 677 589 585 459
357 245 233 210 121 116 31
NaeI GCC'GGC
Cuts at: 2023 3346 5201 2023
Size: 1323 1855 5469
Fragments arranged by size:
5469 1855 1323
NarI GG'CG_CC
Cuts at: 4871 4871
Size: 8647
NcoI C'CATG_G
Cuts at: 513 1065 2004 3915 4851 513
Size: 552 939 1911 936 4309
Fragments arranged by size:
4309 1911 939 936 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8647
NgoMIV G'CCGG_C
Cuts at: 2021 3344 5199 2021
Size: 1323 1855 5469
Fragments arranged by size:
5469 1855 1323
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8647
NotI GC'GGCC_GC
Cuts at: 6094 6094
Size: 8647
NspI r_CATG'y
Cuts at: 1132 1229 2029 2150 2391 3640 6005 7550 1132
Size: 97 800 121 241 1249 2365 1545 2229
Fragments arranged by size:
2365 2229 1545 1249 800 241 121 97
PasI CC'CwG_GG
Cuts at: 1348 2434 4890 1348
Size: 1086 2456 5105
Fragments arranged by size:
5105 2456 1086
PciI A'CATG_T
Cuts at: 7546 7546
Size: 8647
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3088 3372 3375 4300 5597 1108
Size: 1980 284 3 925 1297 4158
Fragments arranged by size:
4158 1980 1297 925 284 3
PfoI T'CCnGG_A
Cuts at: 2289 3087 4254 2289
Size: 798 1167 6682
Fragments arranged by size:
6682 1167 798
PmeI GTTT'AAAC
Cuts at: 5951 5951
Size: 8647
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6716 6748 8229 8261 6716
Size: 32 1481 32 7102
Fragments arranged by size:
7102 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2419 2800 3085 4149 5069 5758 2419
Size: 381 285 1064 920 689 5308
Fragments arranged by size:
5308 1064 920 689 381 285
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8647
PsiI TTA'TAA
Cuts at: 6217 6217
Size: 8647
PspOMI G'GGCC_C
Cuts at: 4893 4893
Size: 8647
PstI C_TGCA'G
Cuts at: 830 2799 3354 3612 3813 5040 5643 5999 830
Size: 1969 555 258 201 1227 603 356 3478
Fragments arranged by size:
3478 1969 1227 603 555 356 258 201
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8647
PvuII CAG'CTG
Cuts at: 2469 4300 5344 5375 5732 5942 2469
Size: 1831 1044 31 357 210 5174
Fragments arranged by size:
5174 1831 1044 357 210 31
SacI G_AGCT'C
Cuts at: 2912 3360 3903 4674 2912
Size: 448 543 771 6885
Fragments arranged by size:
6885 771 543 448
SalI G'TCGA_C
Cuts at: 1936 5989 1936
Size: 4053 4594
SapI GCTCTTCn'nnn_
Cuts at: 3110 3110
Size: 8647
SbfI CC_TGCA'GG
Cuts at: 2799 3354 5999 2799
Size: 555 2645 5447
Fragments arranged by size:
5447 2645 555
ScaI AGT'ACT
Cuts at: 5929 6895 8607 5929
Size: 966 1712 5969
Fragments arranged by size:
5969 1712 966
SexAI A'CCwGG_T
Cuts at: 2817 3081 3371 5352 2817
Size: 264 290 1981 6112
Fragments arranged by size:
6112 1981 290 264
SfcI C'TryA_G
Cuts at: 826 1045 2047 2219 2795 3203 3350 3512 3608
Size: 219 1002 172 576 408 147 162 96
Cuts at: 3608 3809 4624 5036 5060 5639 5895 5995 7130
Size: 201 815 412 24 579 256 100 1135
Cuts at: 7130 7811 8002 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1002 815 681 579 576 412
408 256 219 201 191 172 162 147
100 96 24
SfoI GGC'GCC
Cuts at: 4872 4872
Size: 8647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8647
SgrAI Cr'CCGG_yG
Cuts at: 1700 4377 1700
Size: 2677 5970
SmaI CCC'GGG
Cuts at: 1872 5974 1872
Size: 4102 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3137 4634 4985 5207 6751 7652
Size: 197 926 1194 1497 351 222 1544 901
Cuts at: 7652 7914 820
Size: 262 1553
Fragments arranged by size:
1553 1544 1497 1194 926 901 351 262
222 197
SnaBI TAC'GTA
Cuts at: 493 6438 493
Size: 5945 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8647
SphI G_CATG'C
Cuts at: 1132 6005 1132
Size: 4873 3774
SspI AAT'ATT
Cuts at: 5 52 6571 5
Size: 47 6519 2081
Fragments arranged by size:
6519 2081 47
StuI AGG'CCT
Cuts at: 1517 3758 1517
Size: 2241 6406
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 3915 4851 6086 513
Size: 552 939 9 1902 936 1235 3074
Fragments arranged by size:
3074 1902 1235 939 936 552 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3070 3196 4304 5098 5227 6674 6691
Size: 1255 1490 126 1108 794 129 1447 17
Cuts at: 6691 6844 7029 7474 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1490 1447 1255 1108 794 445 185
153 129 126 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2394 3660 4275
Size: 274 80 33 51 1443 417 1266 615
Cuts at: 4275 5927 6893 8605 96
Size: 1652 966 1712 138
Fragments arranged by size:
1712 1652 1443 1266 966 615 417 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3083 3115 3171 3203 3885 3917 4918 4950 5073
Size: 32 56 32 682 32 1001 32 123
Cuts at: 5073 5105 8607 8639 3083
Size: 32 3502 32 3091
Fragments arranged by size:
3502 3091 1001 682 123 56 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8647
XbaI T'CTAG_A
Cuts at: 5983 5983
Size: 8647
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 3976 4909 2287
Size: 1689 933 6025
Fragments arranged by size:
6025 1689 933
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8647
XmaI C'CCGG_G
Cuts at: 1870 5972 1870
Size: 4102 4545
XmnI GAAnn'nnTTC
Cuts at: 4433 5630 6776 4433
Size: 1197 1146 6304
Fragments arranged by size:
6304 1197 1146
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6455 276
Size: 53 83 186 936 4921 2468
Fragments arranged by size:
4921 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I
EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI
RsrII SacII SanDI SfiI SrfI SwaI