Restriction map for
pFN21AB9503
(Circular) (Six-base) MAPSORT of: pFN21AB9503.seq Check: 9018 from: 1 to: 8509
pFN21AB9503 8509 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5400 6319 278
Size: 53 83 186 936 3864 919 2468
Fragments arranged by size:
3864 2468 936 919 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5181 5631 5852 1937
Size: 3244 450 221 4594
Fragments arranged by size:
4594 3244 450 221
Acc65I G'GTAC_C
Cuts at: 5830 5830
Size: 8509
AclI AA'CG_TT
Cuts at: 1563 6636 7009 1563
Size: 5073 373 3063
Fragments arranged by size:
5073 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3090 4506 6572 7956 8224 1632
Size: 1458 1416 2066 1384 268 1917
Fragments arranged by size:
2066 1917 1458 1416 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8312
AflIII A'CryG_T
Cuts at: 1203 4282 5023 7408 8385 1203
Size: 3079 741 2385 977 1327
Fragments arranged by size:
3079 2385 1327 977 741
AgeI A'CCGG_T
Cuts at: 7340 7340
Size: 8509
AhdI GACnn_n'nnGTC
Cuts at: 7238 7238
Size: 8509
AleI CACnn'nnGTG
Cuts at: 1166 2052 2237 1166
Size: 886 185 7438
Fragments arranged by size:
7438 886 185
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2329 2972 1184
Size: 1145 643 6721
Fragments arranged by size:
6721 1145 643
AlwNI CAG_nnn'CTG
Cuts at: 2465 3163 4153 4321 5339 6252 7824 2465
Size: 698 990 168 1018 913 1572 3150
Fragments arranged by size:
3150 1572 1018 990 913 698 168
ApaI G_GGCC'C
Cuts at: 2943 3242 4118 4676 5801 2943
Size: 299 876 558 1125 5651
Fragments arranged by size:
5651 1125 876 558 299
ApaLI G'TGCA_C
Cuts at: 6566 7722 6566
Size: 1156 7353
ApoI r'AATT_y
Cuts at: 1493 2092 3600 3611 4223 4251 4652 4727 5080
Size: 599 1508 11 612 28 401 75 353
Cuts at: 5080 5471 5750 5818 6044 6196 8305 1493
Size: 391 279 68 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 612 599 401 391 353
279 226 152 75 68 28 11
AseI AT'TA_AT
Cuts at: 160 1032 7063 160
Size: 872 6031 1606
Fragments arranged by size:
6031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8509
AvaI C'yCGr_G
Cuts at: 1870 1943 4704 5834 1870
Size: 73 2761 1130 4545
Fragments arranged by size:
4545 2761 1130 73
BamHI G'GATC_C
Cuts at: 3591 4986 5839 6206 3591
Size: 1395 853 367 5894
Fragments arranged by size:
5894 1395 853 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2603 3348 3368 4013 4556
Size: 325 217 627 816 745 20 645 543
Cuts at: 4556 4939 5032 5435 5665 5830 7285 618
Size: 383 93 403 230 165 1455 1842
Fragments arranged by size:
1842 1455 816 745 645 627 543 403
383 325 230 217 165 93 20
BanII G_rGCy'C
Cuts at: 1391 2943 3242 4118 4676 5509 5541 5770 5801
Size: 1552 299 876 558 833 32 229 31
Cuts at: 5801 5828 1391
Size: 27 4072
Fragments arranged by size:
4072 1552 876 833 558 299 229 32
31 27
BbeI G_GCGC'C
Cuts at: 4560 4560
Size: 8509
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2803 2891 3225 4416 928
Size: 971 904 88 334 1191 5021
Fragments arranged by size:
5021 1191 971 904 334 88
BbvCI CC'TCA_GC
Cuts at: 2458 4302 2458
Size: 1844 6665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3891 3925 4685 4719 6700 6734 1903
Size: 34 1954 34 760 34 1981 34 3678
Fragments arranged by size:
3678 1981 1954 760 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6401 7611 6401
Size: 1210 7299
BclI T'GATC_A
Cuts at: 1548 4323 1548
Size: 2775 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7120 136
Size: 107 122 71 631 605 5448 1525
Fragments arranged by size:
5448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3730 8504 3730
Size: 4774 3735
BlpI GC'TnA_GC
Cuts at: 5926 5926
Size: 8509
Bme1580I G_kGCm'C
Cuts at: 1790 2176 2943 3242 4118 4676 4944 5440 5801
Size: 386 767 299 876 558 268 496 361
Cuts at: 5801 6570 7726 1790
Size: 769 1156 2573
Fragments arranged by size:
2573 1156 876 769 767 558 496 386
361 299 268
BmgBI CAC'GTC
Cuts at: 1318 5254 5605 1318
Size: 3936 351 4222
Fragments arranged by size:
4222 3936 351
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6240 7198 442
Size: 416 53 83 399 24 4823 958 1753
Fragments arranged by size:
4823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8509
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2130 2130
Size: 8509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2208 2276 2838 3000 3254 3518 4296 4394
Size: 969 68 562 162 254 264 778 98
Cuts at: 4394 4925 5129 5603 7169 1239
Size: 531 204 474 1566 2579
Fragments arranged by size:
2579 1566 969 778 562 531 474 264
254 204 162 98 68
Bpu10I CC'TnA_GC
Cuts at: 2458 2838 4302 4620 5037 5769 2458
Size: 380 1464 318 417 732 5198
Fragments arranged by size:
5198 1464 732 417 380 318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 2990 4535 5396 6634 7499 7797 2339
Size: 651 1545 861 1238 865 298 3051
Fragments arranged by size:
3051 1545 1238 865 861 651 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3872 3982 7172 882
Size: 617 2373 110 3190 2219
Fragments arranged by size:
3190 2373 2219 617 110
BsaAI yAC'GTr
Cuts at: 493 1204 4283 6300 493
Size: 711 3079 2017 2702
Fragments arranged by size:
3079 2702 2017 711
BsaBI GATnn'nnATC
Cuts at: 2594 3326 3449 4328 8503 2594
Size: 732 123 879 4175 2600
Fragments arranged by size:
4175 2600 879 732 123
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4347 4557 5397 6316
Size: 53 83 186 936 2814 210 840 919
Cuts at: 6316 6698 7333 275
Size: 382 635 1451
Fragments arranged by size:
2814 1451 936 919 840 635 382 210
186 83 53
BsaWI w'CCGG_w
Cuts at: 2796 3303 5238 6942 7340 7614 7761 8172 2796
Size: 507 1935 1704 398 274 147 411 3133
Fragments arranged by size:
3133 1935 1704 507 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2973 3003 4269 4299 1178
Size: 30 165 30 1570 30 1266 30 5388
Fragments arranged by size:
5388 1570 1266 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2117 2273 2885 3094 4061 4181 4757 5600
Size: 931 156 612 209 967 120 576 843
Cuts at: 5600 1186
Size: 4095
Fragments arranged by size:
4095 967 931 843 612 576 209 156
120
BseYI C'CCAG_C
Cuts at: 2579 2717 2786 3643 4796 5644 5756 7712 8208
Size: 138 69 857 1153 848 112 1956 496
Cuts at: 8208 2579
Size: 2880
Fragments arranged by size:
2880 1956 1153 857 848 496 138 112
69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3494 3980 1163
Size: 573 1758 486 5692
Fragments arranged by size:
5692 1758 573 486
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5959 6720 7748 1540
Size: 275 186 3958 761 1028 2301
Fragments arranged by size:
3958 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2344 4282 6570 6655 7726 2344
Size: 1938 2288 85 1071 3127
Fragments arranged by size:
3127 2288 1938 1071 85
BsmI GAATG_Cn'
Cuts at: 2699 4590 6020 6113 2699
Size: 1891 1430 93 5095
Fragments arranged by size:
5095 1891 1430 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3106 3106
Size: 8509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2176 2344 2943 3242 4118 4282 4676
Size: 399 386 168 599 299 876 164 394
Cuts at: 4676 4944 5440 5509 5541 5770 5801 5828 6570
Size: 268 496 69 32 229 31 27 742
Cuts at: 6570 6655 7726 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 876 742 599 496 399 394
386 299 268 229 168 164 85 69
32 31 27
BspEI T'CCGG_A
Cuts at: 8172 8172
Size: 8509
BspHI T'CATG_A
Cuts at: 2412 6398 8128 2412
Size: 3986 1730 2793
Fragments arranged by size:
3986 2793 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5617 5864 844
Size: 4773 247 3489
Fragments arranged by size:
4773 3489 247
BsrBI CCG'CTC
Cuts at: 1394 1823 3366 4035 5502 6396 1394
Size: 429 1543 669 1467 894 3507
Fragments arranged by size:
3507 1543 1467 894 669 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2895 2954 7004 7178 65
Size: 1183 1647 59 4050 174 1396
Fragments arranged by size:
4050 1647 1396 1183 174 59
BsrFI r'CCGG_y
Cuts at: 1700 1810 7153 7340 1700
Size: 110 5343 187 2869
Fragments arranged by size:
5343 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8509
BssSI C'ACGA_G
Cuts at: 6569 7581 6569
Size: 1012 7497
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2468 4153 794
Size: 1674 1685 5150
Fragments arranged by size:
5150 1685 1674
BstBI TT'CG_AA
Cuts at: 7313 7313
Size: 8509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8509
BstXI CCAn_nnnn'nTGG
Cuts at: 2902 4423 4784 2902
Size: 1521 361 6627
Fragments arranged by size:
6627 1521 361
BstYI r'GATC_y
Cuts at: 1972 2205 2559 2964 2994 3535 3591 3730 4986
Size: 233 354 405 30 541 56 139 1256
Cuts at: 4986 5839 6206 6591 6608 8060 8146 8158 8504
Size: 853 367 385 17 1452 86 12 346
Cuts at: 8504 1972
Size: 1977
Fragments arranged by size:
1977 1452 1256 853 541 405 385 367
354 346 233 139 86 56 30 17
12
BstZ17I GTA'TAC
Cuts at: 5632 5632
Size: 8509
BtgI C'CryG_G
Cuts at: 513 1065 4077 5440 513
Size: 552 3012 1363 3582
Fragments arranged by size:
3582 3012 1363 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2474 2698 3545 5041 487
Size: 683 56 1248 224 847 1496 3955
Fragments arranged by size:
3955 1496 1248 847 683 224 56
BtsI GCAGTG_nn'
Cuts at: 2907 5722 6025 6819 6839 2907
Size: 2815 303 794 20 4577
Fragments arranged by size:
4577 2815 794 303 20
ClaI AT'CG_AT
Cuts at: 1554 4660 1554
Size: 3106 5403
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8474
DraI TTT'AAA
Cuts at: 5813 5813
Size: 8509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7516 809
Size: 507 6200 1802
Fragments arranged by size:
6200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2361 3165 3954
Size: 54 1425 50 275 18 531 804 789
Cuts at: 3954 4867 5956 6845 8474 8
Size: 913 1089 889 1629 43
Fragments arranged by size:
1629 1425 1089 913 889 804 789 531
275 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5956 1537
Size: 275 4144 4090
Fragments arranged by size:
4144 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 3356 3428 4482 5649 6439 1353
Size: 741 1262 72 1054 1167 790 3423
Fragments arranged by size:
3423 1262 1167 1054 790 741 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4178 7096 7470 7616 387
Size: 1064 138 2589 2918 374 146 1280
Fragments arranged by size:
2918 2589 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2208 2276 2838 3000 3090 3254 3518
Size: 393 576 68 562 162 90 164 264
Cuts at: 3518 4296 4394 4506 4925 5129 5603 6572 7169
Size: 778 98 112 419 204 474 969 597
Cuts at: 7169 7956 8224 1239
Size: 787 268 1524
Fragments arranged by size:
1524 969 787 778 597 576 562 474
419 393 268 264 204 164 162 112
98 90 68
EcoNI CCTnn'n_nnAGG
Cuts at: 5673 5673
Size: 8509
EcoO109I rG'GnC_Cy
Cuts at: 3238 3239 3460 3677 4114 4115 4318 4609 4673
Size: 1 221 217 437 1 203 291 64
Cuts at: 4673 5797 5798 3238
Size: 1124 1 5949
Fragments arranged by size:
5949 1124 437 291 221 217 203 64
1 1 1
EcoRI G'AATT_C
Cuts at: 5471 5818 6196 5471
Size: 347 378 7784
Fragments arranged by size:
7784 378 347
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2149 2149
Size: 8509
FspI TGC'GCA
Cuts at: 3413 7015 3413
Size: 3602 4907
FspAI rTGC'GCAy
Cuts at: 3413 3413
Size: 8509
HaeII r_GCGC'y
Cuts at: 1678 4560 5624 7656 1678
Size: 2882 1064 2032 2531
Fragments arranged by size:
2882 2531 2032 1064
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2999 3031 3491 3523 4269
Size: 32 135 32 1427 32 460 32 746
Cuts at: 4269 4301 4452 4484 4817 4849 5030 5062 7150
Size: 32 151 32 333 32 181 32 2088
Cuts at: 7150 7182 7224 7256 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2088 1427 746 460 333 181 151
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 4195 5853 6099 669
Size: 988 281 1128 1129 1658 246 3079
Fragments arranged by size:
3079 1658 1129 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8509
HpaI GTT'AAC
Cuts at: 6099 6099
Size: 8509
KasI G'GCGC_C
Cuts at: 4556 4556
Size: 8509
KpnI G_GTAC'C
Cuts at: 5834 5834
Size: 8509
MfeI C'AATT_G
Cuts at: 6108 6108
Size: 8509
MluI A'CGCG_T
Cuts at: 8385 8385
Size: 8509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2141 2233 5223 7623 7807 1305
Size: 836 92 2990 2400 184 2007
Fragments arranged by size:
2990 2400 2007 836 184 92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 3167 3956 10
Size: 54 1425 343 531 804 789 4563
Fragments arranged by size:
4563 1425 804 789 531 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2052 2237 2387 2900 2928 4421 4633
Size: 648 886 185 150 513 28 1493 212
Cuts at: 4633 6467 6826 6985 518
Size: 1834 359 159 2042
Fragments arranged by size:
2042 1834 1493 886 648 513 359 212
185 159 150 28
MspA1I CmG'CkG
Cuts at: 1606 2186 5404 5442 5925 6602 7750 7995 1606
Size: 580 3218 38 483 677 1148 245 2120
Fragments arranged by size:
3218 2120 1148 677 580 483 245 38
NarI GG'CG_CC
Cuts at: 4557 4557
Size: 8509
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7957
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8509
NotI GC'GGCC_GC
Cuts at: 5956 5956
Size: 8509
NspI r_CATG'y
Cuts at: 1132 1229 3715 5027 5867 7412 1132
Size: 97 2486 1312 840 1545 2229
Fragments arranged by size:
2486 2229 1545 1312 840 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8509
PciI A'CATG_T
Cuts at: 5023 7408 5023
Size: 2385 6124
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4393 4523 1108
Size: 3285 130 5094
Fragments arranged by size:
5094 3285 130
PfoI T'CCnGG_A
Cuts at: 4776 4776
Size: 8509
PmeI GTTT'AAAC
Cuts at: 5813 5813
Size: 8509
PmlI CAC'GTG
Cuts at: 4283 4283
Size: 8509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2326 2358 6578 6610 8091 8123 2326
Size: 32 4220 32 1481 32 2712
Fragments arranged by size:
4220 2712 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3460 3677 4318 4609 3460
Size: 217 641 291 7360
Fragments arranged by size:
7360 641 291 217
PshAI GACnn'nnGTC
Cuts at: 1613 3071 1613
Size: 1458 7051
PsiI TTA'TAA
Cuts at: 6079 6079
Size: 8509
PspOMI G'GGCC_C
Cuts at: 2939 3238 4114 4672 5797 2939
Size: 299 876 558 1125 5651
Fragments arranged by size:
5651 1125 876 558 299
PstI C_TGCA'G
Cuts at: 830 2448 5046 5628 5861 830
Size: 1618 2598 582 233 3478
Fragments arranged by size:
3478 2598 1618 582 233
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8509
SacII CC_GC'GG
Cuts at: 5443 5443
Size: 8509
SalI G'TCGA_C
Cuts at: 1936 5851 1936
Size: 3915 4594
SanDI GG'GwC_CC
Cuts at: 3460 3460
Size: 8509
SapI GCTCTTCn'nnn_
Cuts at: 3356 3428 3356
Size: 72 8437
SbfI CC_TGCA'GG
Cuts at: 5861 5861
Size: 8509
ScaI AGT'ACT
Cuts at: 6757 8469 6757
Size: 1712 6797
SfcI C'TryA_G
Cuts at: 826 1045 2444 3243 5042 5126 5178 5624 5857
Size: 219 1399 799 1799 84 52 446 233
Cuts at: 5857 6992 7673 7864 826
Size: 1135 681 191 1471
Fragments arranged by size:
1799 1471 1399 1135 799 681 446 233
219 191 84 52
SfoI GGC'GCC
Cuts at: 4558 4558
Size: 8509
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8509
SmaI CCC'GGG
Cuts at: 1872 5836 1872
Size: 3964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 2969 4550 5375 6613 7514
Size: 197 926 375 651 1581 825 1238 901
Cuts at: 7514 7776 820
Size: 262 1553
Fragments arranged by size:
1581 1553 1238 926 901 825 651 375
262 197
SnaBI TAC'GTA
Cuts at: 493 6300 493
Size: 5807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8509
SphI G_CATG'C
Cuts at: 1132 5867 1132
Size: 4735 3774
SspI AAT'ATT
Cuts at: 5 52 6433 5
Size: 47 6381 2081
Fragments arranged by size:
6381 2081 47
StuI AGG'CCT
Cuts at: 1517 2376 2456 4379 1517
Size: 859 80 1923 5647
Fragments arranged by size:
5647 1923 859 80
StyI C'CwwG_G
Cuts at: 513 1065 2371 2943 3081 3531 4017 4560 4838
Size: 552 1306 572 138 450 486 543 278
Cuts at: 4838 4893 5094 5661 5792 5948 513
Size: 55 201 567 131 156 3074
Fragments arranged by size:
3074 1306 572 567 552 543 486 450
278 201 156 138 131 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2800 5357 6536 6553 6706 6891 7336
Size: 1255 1220 2557 1179 17 153 185 445
Cuts at: 7336 325
Size: 1498
Fragments arranged by size:
2557 1498 1255 1220 1179 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3396 4760 6755
Size: 274 80 33 51 1443 1419 1364 1995
Cuts at: 6755 8467 96
Size: 1712 138
Fragments arranged by size:
1995 1712 1443 1419 1364 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3352 3384 8469 8501 3352
Size: 32 5085 32 3360
Fragments arranged by size:
5085 3360 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3662 4349 4541 1316
Size: 2346 687 192 5284
Fragments arranged by size:
5284 2346 687 192
XbaI T'CTAG_A
Cuts at: 5845 5845
Size: 8509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2183 4567 4900 5680 2183
Size: 2384 333 780 5012
Fragments arranged by size:
5012 2384 780 333
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8509
XmaI C'CCGG_G
Cuts at: 1870 5834 1870
Size: 3964 4545
XmnI GAAnn'nnTTC
Cuts at: 6638 6638
Size: 8509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5398 6317 276
Size: 53 83 186 936 3864 919 2468
Fragments arranged by size:
3864 2468 936 919 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
Bsu36I DraIII EcoICRI EcoRV FseI NaeI NgoMIV NruI
NsiI PacI PspXI PsrI PvuII RsrII SacI SexAI
SfiI SrfI SwaI