Restriction map for
pFN21AB9502
(Circular) (Six-base) MAPSORT of: pFN21AB9502.seq Check: 5948 from: 1 to: 8224
pFN21AB9502 8224 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6034 278
Size: 53 83 186 936 4498 2468
Fragments arranged by size:
4498 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2386 4274 4881 5567 1937
Size: 449 1888 607 686 4594
Fragments arranged by size:
4594 1888 686 607 449
Acc65I G'GTAC_C
Cuts at: 3293 5512 5545 3293
Size: 2219 33 5972
Fragments arranged by size:
5972 2219 33
AclI AA'CG_TT
Cuts at: 1563 6351 6724 1563
Size: 4788 373 3063
Fragments arranged by size:
4788 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2958 3164 3356 3597 3705 3720 4507 4613
Size: 1326 206 192 241 108 15 787 106
Cuts at: 4613 6287 7671 7939 1632
Size: 1674 1384 268 1917
Fragments arranged by size:
1917 1674 1384 1326 787 268 241 206
192 108 106 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8027
AflIII A'CryG_T
Cuts at: 1203 2196 3979 7123 8100 1203
Size: 993 1783 3144 977 1327
Fragments arranged by size:
3144 1783 1327 993 977
AgeI A'CCGG_T
Cuts at: 7055 7055
Size: 8224
AhdI GACnn_n'nnGTC
Cuts at: 6953 6953
Size: 8224
AleI CACnn'nnGTG
Cuts at: 1166 4937 1166
Size: 3771 4453
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8224
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2116 2148 2116
Size: 32 8192
AlwNI CAG_nnn'CTG
Cuts at: 2621 5522 5967 7539 2621
Size: 2901 445 1572 3306
Fragments arranged by size:
3306 2901 1572 445
ApaLI G'TGCA_C
Cuts at: 3777 6281 7437 3777
Size: 2504 1156 4564
Fragments arranged by size:
4564 2504 1156
ApoI r'AATT_y
Cuts at: 1493 2708 2797 2996 3122 3235 3621 3757 4172
Size: 1215 89 199 126 113 386 136 415
Cuts at: 4172 4184 4260 5533 5759 5911 8020 1493
Size: 12 76 1273 226 152 2109 1697
Fragments arranged by size:
2109 1697 1273 1215 415 386 226 199
152 136 126 113 89 76 12
AseI AT'TA_AT
Cuts at: 160 1032 6778 160
Size: 872 5746 1606
Fragments arranged by size:
5746 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8224
AvaI C'yCGr_G
Cuts at: 1870 1943 2056 5549 1870
Size: 73 113 3493 4545
Fragments arranged by size:
4545 3493 113 73
BamHI G'GATC_C
Cuts at: 4548 5554 5921 4548
Size: 1006 367 6851
Fragments arranged by size:
6851 1006 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2314 2676 3226 3293 4939
Size: 325 217 627 527 362 550 67 1646
Cuts at: 4939 5512 5545 7000 618
Size: 573 33 1455 1842
Fragments arranged by size:
1842 1646 1455 627 573 550 527 362
325 217 67 33
BanII G_rGCy'C
Cuts at: 1391 2333 5543 1391
Size: 942 3210 4072
Fragments arranged by size:
4072 3210 942
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3152 4041 4083 4134 4653 5225 928
Size: 971 1253 889 42 51 519 572 3927
Fragments arranged by size:
3927 1253 971 889 572 519 51 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6415 6449 1903
Size: 34 4478 34 3678
Fragments arranged by size:
4478 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3710 5436 6116 7326 3710
Size: 1726 680 1210 4608
Fragments arranged by size:
4608 1726 1210 680
BclI T'GATC_A
Cuts at: 1548 4213 1548
Size: 2665 5559
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2029 6835 136
Size: 107 122 71 631 605 357 4806 1525
Fragments arranged by size:
4806 1525 631 605 357 122 107 71
BglII A'GATC_T
Cuts at: 3034 3953 4604 8219 3034
Size: 919 651 3615 3039
Fragments arranged by size:
3615 3039 919 651
BlpI GC'TnA_GC
Cuts at: 5641 5641
Size: 8224
Bme1580I G_kGCm'C
Cuts at: 1790 3781 6285 7441 1790
Size: 1991 2504 1156 2573
Fragments arranged by size:
2573 2504 1991 1156
BmgBI CAC'GTC
Cuts at: 1318 2399 1318
Size: 1081 7143
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2265 2708 5955
Size: 416 53 83 399 24 848 443 3247
Cuts at: 5955 6913 442
Size: 958 1753
Fragments arranged by size:
3247 1753 958 848 443 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3366 3393 1056
Size: 2310 27 5887
Fragments arranged by size:
5887 2310 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3538 3538
Size: 8224
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2626 4640 4957 6884 1239
Size: 1387 2014 317 1927 2579
Fragments arranged by size:
2579 2014 1927 1387 317
Bpu10I CC'TnA_GC
Cuts at: 2651 2651
Size: 8224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2920 3107 6349 7214 7512 2920
Size: 187 3242 865 298 3632
Fragments arranged by size:
3632 3242 865 298 187
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2080 6887 882
Size: 617 581 4807 2219
Fragments arranged by size:
4807 2219 617 581
BsaAI yAC'GTr
Cuts at: 493 1204 5030 6015 493
Size: 711 3826 985 2702
Fragments arranged by size:
3826 2702 985 711
BsaBI GATnn'nnATC
Cuts at: 2568 4218 8218 2568
Size: 1650 4000 2574
Fragments arranged by size:
4000 2574 1650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6031 6413 7048 275
Size: 53 83 186 936 4498 382 635 1451
Fragments arranged by size:
4498 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6657 7055 7329 7476 7887 6657
Size: 398 274 147 411 6994
Fragments arranged by size:
6994 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2474 2504 3652 3682 1178
Size: 30 165 30 1071 30 1148 30 5720
Fragments arranged by size:
5720 1148 1071 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 8224
BseYI C'CCAG_C
Cuts at: 2050 7427 7923 2050
Size: 5377 496 2351
Fragments arranged by size:
5377 2351 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4496 5171 1163
Size: 573 2760 675 4216
Fragments arranged by size:
4216 2760 675 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5674 6435 7463 1540
Size: 275 186 3673 761 1028 2301
Fragments arranged by size:
3673 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2370 3490 3781 4167 6285 6370 7441 2370
Size: 1120 291 386 2118 85 1071 3153
Fragments arranged by size:
3153 2118 1120 1071 386 291 85
BsmI GAATG_Cn'
Cuts at: 3544 5735 5828 3544
Size: 2191 93 5940
Fragments arranged by size:
5940 2191 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2370 3490 3781 4167 5543 6285
Size: 399 543 37 1120 291 386 1376 742
Cuts at: 6285 6370 7441 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1376 1120 1071 742 543 399 386
291 85 37
BspEI T'CCGG_A
Cuts at: 7887 7887
Size: 8224
BspHI T'CATG_A
Cuts at: 2349 2542 5500 6113 7843 2349
Size: 193 2958 613 1730 2730
Fragments arranged by size:
2958 2730 1730 613 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3281 3758 5113 5579 844
Size: 2437 477 1355 466 3489
Fragments arranged by size:
3489 2437 1355 477 466
BsrBI CCG'CTC
Cuts at: 1394 1823 6111 1394
Size: 429 4288 3507
Fragments arranged by size:
4288 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6719 6893 65
Size: 1183 5471 174 1396
Fragments arranged by size:
5471 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6868 7055 1700
Size: 110 5058 187 2869
Fragments arranged by size:
5058 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4775 96
Size: 4679 3545
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8224
BssSI C'ACGA_G
Cuts at: 6284 7296 6284
Size: 1012 7212
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8224
BstBI TT'CG_AA
Cuts at: 7028 7028
Size: 8224
BstEII G'GTnAC_C
Cuts at: 1184 4796 1184
Size: 3612 4612
BstYI r'GATC_y
Cuts at: 1972 2945 3034 3953 4548 4604 5554 5921 6306
Size: 973 89 919 595 56 950 367 385
Cuts at: 6306 6323 7775 7861 7873 8219 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 973 950 919 595 385 367
346 89 86 56 17 12
BstZ17I GTA'TAC
Cuts at: 2387 4275 2387
Size: 1888 6336
BtgI C'CryG_G
Cuts at: 513 1065 4544 513
Size: 552 3479 4193
Fragments arranged by size:
4193 3479 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5123 487
Size: 683 56 3897 3588
Fragments arranged by size:
3897 3588 683 56
BtsI GCAGTG_nn'
Cuts at: 3781 5740 6534 6554 3781
Size: 1959 794 20 5451
Fragments arranged by size:
5451 1959 794 20
ClaI AT'CG_AT
Cuts at: 1554 2509 1554
Size: 955 7269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8189
DraI TTT'AAA
Cuts at: 4749 4840 5528 4749
Size: 91 688 7445
Fragments arranged by size:
7445 688 91
DraIII CAC_nnn'GTG
Cuts at: 3155 5344 3155
Size: 2189 6035
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7231 809
Size: 507 5915 1802
Fragments arranged by size:
5915 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3250 4541 5671
Size: 54 1425 50 275 18 1420 1291 1130
Cuts at: 5671 6560 8189 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1420 1291 1130 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5671 1537
Size: 275 3859 4090
Fragments arranged by size:
4090 3859 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2956 3217 3495 3849 4155 6154 1353
Size: 1603 261 278 354 306 1999 3423
Fragments arranged by size:
3423 1999 1603 354 306 278 261
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6811 7185 7331 387
Size: 1064 138 5222 374 146 1280
Fragments arranged by size:
5222 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2626 2958 3164 3356 3597 3705 3720
Size: 393 994 332 206 192 241 108 15
Cuts at: 3720 4507 4613 4640 4957 6287 6884 7671 7939
Size: 787 106 27 317 1330 597 787 268
Cuts at: 7939 1239
Size: 1524
Fragments arranged by size:
1524 1330 994 787 787 597 393 332
317 268 241 206 192 108 106 27
15
EcoNI CCTnn'n_nnAGG
Cuts at: 3738 3738
Size: 8224
EcoO109I rG'GnC_Cy
Cuts at: 3632 3632
Size: 8224
EcoRI G'AATT_C
Cuts at: 2797 5533 5911 2797
Size: 2736 378 5110
Fragments arranged by size:
5110 2736 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2967 4397 2967
Size: 1430 6794
FspI TGC'GCA
Cuts at: 6730 6730
Size: 8224
HaeII r_GCGC'y
Cuts at: 1678 2017 7371 1678
Size: 339 5354 2531
Fragments arranged by size:
5354 2531 339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2235 2267 2474 2506 4589
Size: 32 135 32 663 32 207 32 2083
Cuts at: 4589 4621 5037 5069 6865 6897 6939 6971 1373
Size: 32 416 32 1796 32 42 32 2626
Fragments arranged by size:
2626 2083 1796 663 416 207 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5568 5814 669
Size: 988 281 3630 246 3079
Fragments arranged by size:
3630 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2343 5075 748
Size: 1595 2732 3897
Fragments arranged by size:
3897 2732 1595
HpaI GTT'AAC
Cuts at: 5814 5814
Size: 8224
KpnI G_GTAC'C
Cuts at: 3297 5516 5549 3297
Size: 2219 33 5972
Fragments arranged by size:
5972 2219 33
MfeI C'AATT_G
Cuts at: 4197 5823 4197
Size: 1626 6598
MluI A'CGCG_T
Cuts at: 8100 8100
Size: 8224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2181 7338 7522 1305
Size: 876 5157 184 2007
Fragments arranged by size:
5157 2007 876 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3252 4543 10
Size: 54 1425 343 1420 1291 3691
Fragments arranged by size:
3691 1425 1420 1291 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4224 4937 5097 6182 6541 6700 518
Size: 648 3058 713 160 1085 359 159 2042
Fragments arranged by size:
3058 2042 1085 713 648 359 160 159
MspA1I CmG'CkG
Cuts at: 1606 2114 4574 4861 5008 5640 6317 7465 7710
Size: 508 2460 287 147 632 677 1148 245
Cuts at: 7710 1606
Size: 2120
Fragments arranged by size:
2460 2120 1148 677 632 508 287 245
147
NcoI C'CATG_G
Cuts at: 513 1065 4544 513
Size: 552 3479 4193
Fragments arranged by size:
4193 3479 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8224
NheI G'CTAG_C
Cuts at: 1052 3362 3389 1052
Size: 2310 27 5887
Fragments arranged by size:
5887 2310 27
NotI GC'GGCC_GC
Cuts at: 5671 5671
Size: 8224
NspI r_CATG'y
Cuts at: 1132 1229 2023 2200 3983 5375 5582 7127 1132
Size: 97 794 177 1783 1392 207 1545 2229
Fragments arranged by size:
2229 1783 1545 1392 794 207 177 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8224
PciI A'CATG_T
Cuts at: 2196 3979 7123 2196
Size: 1783 3144 3297
Fragments arranged by size:
3297 3144 1783
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3449 1108
Size: 2341 5883
PfoI T'CCnGG_A
Cuts at: 3988 3988
Size: 8224
PmeI GTTT'AAAC
Cuts at: 5528 5528
Size: 8224
PmlI CAC'GTG
Cuts at: 5030 5030
Size: 8224
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6293 6325 7806 7838 6293
Size: 32 1481 32 6679
Fragments arranged by size:
6679 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3632 3632
Size: 8224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8224
PsiI TTA'TAA
Cuts at: 3128 4710 5794 3128
Size: 1582 1084 5558
Fragments arranged by size:
5558 1582 1084
PstI C_TGCA'G
Cuts at: 830 2113 2738 3220 3715 3916 4062 5124 5576
Size: 1283 625 482 495 201 146 1062 452
Cuts at: 5576 830
Size: 3478
Fragments arranged by size:
3478 1283 1062 625 495 482 452 201
146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8224
PvuII CAG'CTG
Cuts at: 4574 4861 5008 4574
Size: 287 147 7790
Fragments arranged by size:
7790 287 147
SalI G'TCGA_C
Cuts at: 1936 5566 1936
Size: 3630 4594
SapI GCTCTTCn'nnn_
Cuts at: 3217 3495 4155 3217
Size: 278 660 7286
Fragments arranged by size:
7286 660 278
SbfI CC_TGCA'GG
Cuts at: 5576 5576
Size: 8224
ScaI AGT'ACT
Cuts at: 6472 8184 6472
Size: 1712 6512
SexAI A'CCwGG_T
Cuts at: 3151 3151
Size: 8224
SfcI C'TryA_G
Cuts at: 826 1045 2109 2734 3216 3549 3711 3912 3918
Size: 219 1064 625 482 333 162 201 6
Cuts at: 3918 4058 4317 5120 5572 6707 7388 7579 826
Size: 140 259 803 452 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 803 681 625 482 452
333 259 219 201 191 162 140 6
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8224
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8224
SmaI CCC'GGG
Cuts at: 1872 2058 5551 1872
Size: 186 3493 4545
Fragments arranged by size:
4545 3493 186
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2935 3086 6328 7229 7491 820
Size: 197 926 992 151 3242 901 262 1553
Fragments arranged by size:
3242 1553 992 926 901 262 197 151
SnaBI TAC'GTA
Cuts at: 493 6015 493
Size: 5522 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8224
SphI G_CATG'C
Cuts at: 1132 2023 5582 1132
Size: 891 3559 3774
Fragments arranged by size:
3774 3559 891
SspI AAT'ATT
Cuts at: 5 52 2429 4236 6148 5
Size: 47 2377 1807 1912 2081
Fragments arranged by size:
2377 2081 1912 1807 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8224
StyI C'CwwG_G
Cuts at: 513 1065 2858 3183 3297 3942 4278 4544 5663
Size: 552 1793 325 114 645 336 266 1119
Cuts at: 5663 513
Size: 3074
Fragments arranged by size:
3074 1793 1119 645 552 336 325 266
114
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6251 6268 6421 6606 7051 325
Size: 1255 4671 17 153 185 445 1498
Fragments arranged by size:
4671 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4133 4487 4775
Size: 274 80 33 51 1443 2156 354 288
Cuts at: 4775 6470 8182 96
Size: 1695 1712 138
Fragments arranged by size:
2156 1712 1695 1443 354 288 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4362 4394 4678 4710 5014 5046 8184 8216 4362
Size: 32 284 32 304 32 3138 32 4370
Fragments arranged by size:
4370 3138 304 284 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5432 1316
Size: 4116 4108
XbaI T'CTAG_A
Cuts at: 5560 5560
Size: 8224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2378 4542 2378
Size: 2164 6060
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8224
XmaI C'CCGG_G
Cuts at: 1870 2056 5549 1870
Size: 186 3493 4545
Fragments arranged by size:
4545 3493 186
XmnI GAAnn'nnTTC
Cuts at: 2801 2996 5100 6353 2801
Size: 195 2104 1253 4672
Fragments arranged by size:
4672 2104 1253 195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6032 276
Size: 53 83 186 936 4498 2468
Fragments arranged by size:
4498 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI
SfoI SrfI SwaI