Restriction map for
pFN21AB9498
(Circular) (Six-base) MAPSORT of: pFN21AB9498.seq Check: 4954 from: 1 to: 6538
pFN21AB9498 6538 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4348 278
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3881 1937
Size: 1944 4594
Acc65I G'GTAC_C
Cuts at: 3859 3859
Size: 6538
AclI AA'CG_TT
Cuts at: 1563 4665 5038 1563
Size: 3102 373 3063
Fragments arranged by size:
3102 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4601 5985 6253 1632
Size: 2969 1384 268 1917
Fragments arranged by size:
2969 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6341
AflIII A'CryG_T
Cuts at: 1203 5437 6414 1203
Size: 4234 977 1327
Fragments arranged by size:
4234 1327 977
AgeI A'CCGG_T
Cuts at: 2697 5369 2697
Size: 2672 3866
AhdI GACnn_n'nnGTC
Cuts at: 5267 5267
Size: 6538
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6538
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6538
AlwNI CAG_nnn'CTG
Cuts at: 4281 5853 4281
Size: 1572 4966
ApaLI G'TGCA_C
Cuts at: 4595 5751 4595
Size: 1156 5382
ApoI r'AATT_y
Cuts at: 1493 2345 2432 2499 3847 4073 4225 6334 1493
Size: 852 87 67 1348 226 152 2109 1697
Fragments arranged by size:
2109 1697 1348 852 226 152 87 67
AseI AT'TA_AT
Cuts at: 160 1032 5092 160
Size: 872 4060 1606
Fragments arranged by size:
4060 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
AvaI C'yCGr_G
Cuts at: 1870 1943 2244 2955 3069 3204 3863 1870
Size: 73 301 711 114 135 659 4545
Fragments arranged by size:
4545 711 659 301 135 114 73
BamHI G'GATC_C
Cuts at: 3868 4235 3868
Size: 367 6171
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2197 3859 5314 618
Size: 325 217 627 410 1662 1455 1842
Fragments arranged by size:
1842 1662 1455 627 410 325 217
BanII G_rGCy'C
Cuts at: 1391 2608 2939 3497 3857 1391
Size: 1217 331 558 360 4072
Fragments arranged by size:
4072 1217 558 360 331
BbeI G_GCGC'C
Cuts at: 2201 2201
Size: 6538
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2048 2264 2907 928
Size: 971 149 216 643 4559
Fragments arranged by size:
4559 971 643 216 149
BbvCI CC'TCA_GC
Cuts at: 2014 2070 2014
Size: 56 6482
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4729 4763 1903
Size: 34 2792 34 3678
Fragments arranged by size:
3678 2792 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4430 5640 4430
Size: 1210 5328
BclI T'GATC_A
Cuts at: 1548 2518 3406 1548
Size: 970 888 4680
Fragments arranged by size:
4680 970 888
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5149 136
Size: 107 122 71 631 605 337 3140 1525
Fragments arranged by size:
3140 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3716 6533 3716
Size: 2817 3721
BlpI GC'TnA_GC
Cuts at: 3955 3955
Size: 6538
Bme1580I G_kGCm'C
Cuts at: 1790 2464 4599 5755 1790
Size: 674 2135 1156 2573
Fragments arranged by size:
2573 2135 1156 674
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6538
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3055 3294 3633
Size: 416 53 83 399 24 1638 239 339
Cuts at: 3633 4269 5227 442
Size: 636 958 1753
Fragments arranged by size:
1753 1638 958 636 416 399 339 239
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2620 2923 3528 3697 5198 1239
Size: 1381 303 605 169 1501 2579
Fragments arranged by size:
2579 1501 1381 605 303 169
Bpu10I CC'TnA_GC
Cuts at: 2014 2070 3726 2014
Size: 56 1656 4826
Fragments arranged by size:
4826 1656 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4663 5528 5826 4663
Size: 865 298 5375
Fragments arranged by size:
5375 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3657 5201 882
Size: 617 2158 1544 2219
Fragments arranged by size:
2219 2158 1544 617
BsaAI yAC'GTr
Cuts at: 493 1204 2387 4329 493
Size: 711 1183 1942 2702
Fragments arranged by size:
2702 1942 1183 711
BsaBI GATnn'nnATC
Cuts at: 2568 2712 2724 2751 6532 2568
Size: 144 12 27 3781 2574
Fragments arranged by size:
3781 2574 144 27 12
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2198 4345 4727 5362
Size: 53 83 186 936 665 2147 382 635
Cuts at: 5362 275
Size: 1451
Fragments arranged by size:
2147 1451 936 665 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2262 2697 4971 5369 5643 5790 6201 2262
Size: 435 2274 398 274 147 411 2599
Fragments arranged by size:
2599 2274 435 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2593 2623 3313 3343 1178
Size: 30 165 30 1190 30 690 30 4373
Fragments arranged by size:
4373 1190 690 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2895 1186
Size: 1709 4829
BseYI C'CCAG_C
Cuts at: 2121 2393 5741 6237 2121
Size: 272 3348 496 2422
Fragments arranged by size:
3348 2422 496 272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2391 3705 1163
Size: 573 655 1314 3996
Fragments arranged by size:
3996 1314 655 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2263 2930 3257 3988 4749 5777
Size: 275 186 262 667 327 731 761 1028
Cuts at: 5777 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 731 667 327 275 262
186
BsiHKAI G_wGCw'C
Cuts at: 2939 3497 4599 4684 5755 2939
Size: 558 1102 85 1071 3722
Fragments arranged by size:
3722 1102 1071 558 85
BsmI GAATG_Cn'
Cuts at: 3454 3610 4049 4142 3454
Size: 156 439 93 5850
Fragments arranged by size:
5850 439 156 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3642 3642
Size: 6538
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2464 2608 2939 3497 3857 4599 4684
Size: 399 674 144 331 558 360 742 85
Cuts at: 4684 5755 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 674 558 399 360 331
144 85
BspEI T'CCGG_A
Cuts at: 6201 6201
Size: 6538
BspHI T'CATG_A
Cuts at: 4427 6157 4427
Size: 1730 4808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3893 844
Size: 3049 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4425 1394
Size: 429 2602 3507
Fragments arranged by size:
3507 2602 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5033 5207 65
Size: 1183 3785 174 1396
Fragments arranged by size:
3785 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2697 5182 5369 1700
Size: 110 887 2485 187 2869
Fragments arranged by size:
2869 2485 887 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6538
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6538
BssSI C'ACGA_G
Cuts at: 4598 5610 4598
Size: 1012 5526
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6538
BstBI TT'CG_AA
Cuts at: 5342 5342
Size: 6538
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6538
BstXI CCAn_nnnn'nTGG
Cuts at: 2121 2121
Size: 6538
BstYI r'GATC_y
Cuts at: 1972 3716 3868 4235 4620 4637 6089 6175 6187
Size: 1744 152 367 385 17 1452 86 12
Cuts at: 6187 6533 1972
Size: 346 1977
Fragments arranged by size:
1977 1744 1452 385 367 346 152 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3030 3741 3030
Size: 711 5827
BtgI C'CryG_G
Cuts at: 513 1065 2004 2234 3637 513
Size: 552 939 230 1403 3414
Fragments arranged by size:
3414 1403 939 552 230
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5799
Fragments arranged by size:
5799 683 56
BtsI GCAGTG_nn'
Cuts at: 2226 2292 2379 2836 2857 4054 4848 4868 2226
Size: 66 87 457 21 1197 794 20 3896
Fragments arranged by size:
3896 1197 794 457 87 66 21 20
ClaI AT'CG_AT
Cuts at: 1554 2755 1554
Size: 1201 5337
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6503
DraI TTT'AAA
Cuts at: 3842 3842
Size: 6538
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5545 809
Size: 507 4229 1802
Fragments arranged by size:
4229 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2557 2764 3985
Size: 54 1425 50 275 18 727 207 1221
Cuts at: 3985 4874 6503 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1221 889 727 275 207 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3985 1537
Size: 275 2173 4090
Fragments arranged by size:
4090 2173 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 2472 2641 2833 4468 1353
Size: 972 147 169 192 1635 3423
Fragments arranged by size:
3423 1635 972 192 169 147
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5125 5499 5645 387
Size: 1064 138 3536 374 146 1280
Fragments arranged by size:
3536 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2937 3495 2937
Size: 558 5980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2620 2923 3528 3697 4601 5198 5985
Size: 393 988 303 605 169 904 597 787
Cuts at: 5985 6253 1239
Size: 268 1524
Fragments arranged by size:
1524 988 904 787 605 597 393 303
268 169
EcoNI CCTnn'n_nnAGG
Cuts at: 2646 2646
Size: 6538
EcoO109I rG'GnC_Cy
Cuts at: 2440 2984 3288 2440
Size: 544 304 5690
Fragments arranged by size:
5690 544 304
EcoRI G'AATT_C
Cuts at: 2345 2499 3847 4225 2345
Size: 154 1348 378 4658
Fragments arranged by size:
4658 1348 378 154
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 6538
FspI TGC'GCA
Cuts at: 5044 5044
Size: 6538
HaeII r_GCGC'y
Cuts at: 1678 2201 5685 1678
Size: 523 3484 2531
Fragments arranged by size:
3484 2531 523
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2349 2381 2593 2625 2912
Size: 32 135 32 777 32 212 32 287
Cuts at: 2912 2936 2944 2968 3029 3061 3362 3394 3497
Size: 24 8 24 61 32 301 32 103
Cuts at: 3497 3529 3695 3701 3727 3733 5179 5211 5253
Size: 32 166 6 26 6 1446 32 42
Cuts at: 5253 5285 1373
Size: 32 2626
Fragments arranged by size:
2626 1446 777 301 287 212 166 135
103 61 42 32 32 32 32 32
32 32 32 32 26 24 24 8
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 3882 4128 669
Size: 988 281 1168 776 246 3079
Fragments arranged by size:
3079 1168 988 776 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6538
HpaI GTT'AAC
Cuts at: 4128 4128
Size: 6538
KasI G'GCGC_C
Cuts at: 2197 2197
Size: 6538
KpnI G_GTAC'C
Cuts at: 3863 3863
Size: 6538
MfeI C'AATT_G
Cuts at: 2205 4137 2205
Size: 1932 4606
MluI A'CGCG_T
Cuts at: 6414 6414
Size: 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3407 5652 5836 1305
Size: 2102 2245 184 2007
Fragments arranged by size:
2245 2102 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4716
Fragments arranged by size:
4716 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4496 4855 5014 518
Size: 648 3330 359 159 2042
Fragments arranged by size:
3330 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2121 3113 3175 3954 4631 5779 6024 1606
Size: 515 992 62 779 677 1148 245 2120
Fragments arranged by size:
2120 1148 992 779 677 515 245 62
NarI GG'CG_CC
Cuts at: 2198 2198
Size: 6538
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5047
Fragments arranged by size:
5047 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6538
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6538
NotI GC'GGCC_GC
Cuts at: 3985 3985
Size: 6538
NspI r_CATG'y
Cuts at: 1132 1229 3896 5441 1132
Size: 97 2667 1545 2229
Fragments arranged by size:
2667 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6538
PciI A'CATG_T
Cuts at: 5437 5437
Size: 6538
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6538
PfoI T'CCnGG_A
Cuts at: 2819 3643 2819
Size: 824 5714
PmeI GTTT'AAAC
Cuts at: 3842 3842
Size: 6538
PmlI CAC'GTG
Cuts at: 2387 2387
Size: 6538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 6120 6152 4607
Size: 32 1481 32 4993
Fragments arranged by size:
4993 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 2984 3288 2440
Size: 544 304 5690
Fragments arranged by size:
5690 544 304
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6538
PsiI TTA'TAA
Cuts at: 4108 4108
Size: 6538
PspXI vC'TCGA_Gb
Cuts at: 3069 3069
Size: 6538
PstI C_TGCA'G
Cuts at: 830 3890 830
Size: 3060 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6538
PvuII CAG'CTG
Cuts at: 2121 3113 3175 2121
Size: 992 62 5484
Fragments arranged by size:
5484 992 62
SacI G_AGCT'C
Cuts at: 2939 3497 2939
Size: 558 5980
SalI G'TCGA_C
Cuts at: 1936 3880 1936
Size: 1944 4594
SapI GCTCTTCn'nnn_
Cuts at: 2833 2833
Size: 6538
SbfI CC_TGCA'GG
Cuts at: 3890 3890
Size: 6538
ScaI AGT'ACT
Cuts at: 3025 4786 6498 3025
Size: 1761 1712 3065
Fragments arranged by size:
3065 1761 1712
SfcI C'TryA_G
Cuts at: 826 1045 2616 2875 3143 3886 5021 5702 5893
Size: 219 1571 259 268 743 1135 681 191
Cuts at: 5893 826
Size: 1471
Fragments arranged by size:
1571 1471 1135 743 681 268 259 219
191
SfoI GGC'GCC
Cuts at: 2199 2199
Size: 6538
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6538
SmaI CCC'GGG
Cuts at: 1872 3865 1872
Size: 1993 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3069 4642 5543 5805 820
Size: 197 926 1126 1573 901 262 1553
Fragments arranged by size:
1573 1553 1126 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4329 493
Size: 3836 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6538
SphI G_CATG'C
Cuts at: 1132 3896 1132
Size: 2764 3774
SspI AAT'ATT
Cuts at: 5 52 4462 5
Size: 47 4410 2081
Fragments arranged by size:
4410 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6538
StyI C'CwwG_G
Cuts at: 513 1065 2004 3977 513
Size: 552 939 1973 3074
Fragments arranged by size:
3074 1973 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2799 2916 2919 3231 3445 4565 4582
Size: 1255 1219 117 3 312 214 1120 17
Cuts at: 4582 4735 4920 5365 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1219 1120 445 312 214 185
153 117 17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3023 4784 6496
Size: 274 80 33 51 1443 1046 1761 1712
Cuts at: 6496 96
Size: 138
Fragments arranged by size:
1761 1712 1443 1046 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2815 2847 3253 3285 3313 3345 6498 6530 2815
Size: 32 406 32 28 32 3153 32 2823
Fragments arranged by size:
3153 2823 406 32 32 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6538
XbaI T'CTAG_A
Cuts at: 3340 3874 3340
Size: 534 6004
XhoI C'TCGA_G
Cuts at: 1943 3069 1943
Size: 1126 5412
XmaI C'CCGG_G
Cuts at: 1870 3863 1870
Size: 1993 4545
XmnI GAAnn'nnTTC
Cuts at: 2349 4667 2349
Size: 2318 4220
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4346 276
Size: 53 83 186 936 2812 2468
Fragments arranged by size:
2812 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacII
SanDI SexAI SfiI SrfI SwaI XcmI