Restriction map for
pFN21AB9490
(Circular) (Six-base) MAPSORT of: pFN21AB9490.seq Check: 9468 from: 1 to: 8269
pFN21AB9490 8269 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4362 5017 4362
Size: 655 7614
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6079 278
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5612 1937
Size: 3675 4594
Acc65I G'GTAC_C
Cuts at: 2133 5590 2133
Size: 3457 4812
AclI AA'CG_TT
Cuts at: 1563 6396 6769 1563
Size: 4833 373 3063
Fragments arranged by size:
4833 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3740 4464 5223 6332 7716 7984 1632
Size: 2108 724 759 1109 1384 268 1917
Fragments arranged by size:
2108 1917 1384 1109 759 724 268
AflII C'TTAA_G
Cuts at: 820 1017 2704 820
Size: 197 1687 6385
Fragments arranged by size:
6385 1687 197
AflIII A'CryG_T
Cuts at: 1203 2033 2845 7168 8145 1203
Size: 830 812 4323 977 1327
Fragments arranged by size:
4323 1327 977 830 812
AgeI A'CCGG_T
Cuts at: 7100 7100
Size: 8269
AhdI GACnn_n'nnGTC
Cuts at: 4576 6998 4576
Size: 2422 5847
AleI CACnn'nnGTG
Cuts at: 1166 5529 1166
Size: 4363 3906
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3627 1184
Size: 2443 5826
AlwNI CAG_nnn'CTG
Cuts at: 4332 6012 7584 4332
Size: 1680 1572 5017
Fragments arranged by size:
5017 1680 1572
ApaLI G'TGCA_C
Cuts at: 6326 7482 6326
Size: 1156 7113
ApoI r'AATT_y
Cuts at: 1493 2052 2504 2556 2574 2613 2743 2884 5099
Size: 559 452 52 18 39 130 141 2215
Cuts at: 5099 5192 5463 5578 5804 5956 8065 1493
Size: 93 271 115 226 152 2109 1697
Fragments arranged by size:
2215 2109 1697 559 452 271 226 152
141 130 115 93 52 39 18
AseI AT'TA_AT
Cuts at: 160 1032 6823 160
Size: 872 5791 1606
Fragments arranged by size:
5791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
AvaI C'yCGr_G
Cuts at: 1870 1943 3420 3998 4988 5594 1870
Size: 73 1477 578 990 606 4545
Fragments arranged by size:
4545 1477 990 606 578 73
BamHI G'GATC_C
Cuts at: 2982 5599 5966 2982
Size: 2617 367 5285
Fragments arranged by size:
5285 2617 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2133 2637 3312 5590 7045
Size: 325 217 627 346 504 675 2278 1455
Cuts at: 7045 618
Size: 1842
Fragments arranged by size:
2278 1842 1455 675 627 504 346 325
217
BanII G_rGCy'C
Cuts at: 1391 4553 5181 5296 5352 5588 1391
Size: 3162 628 115 56 236 4072
Fragments arranged by size:
4072 3162 628 236 115 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2862 3645 3746 4470 4593 5229 5385
Size: 971 963 783 101 724 123 636 156
Cuts at: 5385 928
Size: 3812
Fragments arranged by size:
3812 971 963 783 724 636 156 123
101
BbvCI CC'TCA_GC
Cuts at: 5327 5327
Size: 8269
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3485 3519 6460 6494 1903
Size: 34 1548 34 2941 34 3678
Fragments arranged by size:
3678 2941 1548 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2633 6161 7371 2633
Size: 3528 1210 3531
Fragments arranged by size:
3531 3528 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8269
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6880 136
Size: 107 122 71 631 605 5208 1525
Fragments arranged by size:
5208 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2227 2932 3586 4819 8264 2227
Size: 705 654 1233 3445 2232
Fragments arranged by size:
3445 2232 1233 705 654
BlpI GC'TnA_GC
Cuts at: 2776 5686 2776
Size: 2910 5359
Bme1580I G_kGCm'C
Cuts at: 1790 6330 7486 1790
Size: 4540 1156 2573
Fragments arranged by size:
4540 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4573 4679 1318
Size: 3255 106 4908
Fragments arranged by size:
4908 3255 106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3043 3741 6000
Size: 416 53 83 399 24 1626 698 2259
Cuts at: 6000 6958 442
Size: 958 1753
Fragments arranged by size:
2259 1753 1626 958 698 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8269
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3675 3912 4132 6929 1239
Size: 2436 237 220 2797 2579
Fragments arranged by size:
2797 2579 2436 237 220
Bpu10I CC'TnA_GC
Cuts at: 2633 5327 2633
Size: 2694 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2369 3259 6394 7259 7557 2369
Size: 890 3135 865 298 3081
Fragments arranged by size:
3135 3081 890 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6932 882
Size: 617 5433 2219
Fragments arranged by size:
5433 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6060 493
Size: 711 4856 2702
Fragments arranged by size:
4856 2702 711
BsaBI GATnn'nnATC
Cuts at: 4104 4853 8263 4104
Size: 749 3410 4110
Fragments arranged by size:
4110 3410 749
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6076 6458 7093 275
Size: 53 83 186 936 4543 382 635 1451
Fragments arranged by size:
4543 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5121 6702 7100 7374 7521 7932 5121
Size: 1581 398 274 147 411 5458
Fragments arranged by size:
5458 1581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2857 2887 3467 3497 3651
Size: 30 165 30 1454 30 580 30 154
Cuts at: 3651 3681 3885 3915 5535 5565 1178
Size: 30 204 30 1620 30 3882
Fragments arranged by size:
3882 1620 1454 580 204 165 154 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3581 4310 1186
Size: 2395 729 5145
Fragments arranged by size:
5145 2395 729
BseYI C'CCAG_C
Cuts at: 3362 4109 4486 4554 7472 7968 3362
Size: 747 377 68 2918 496 3663
Fragments arranged by size:
3663 2918 747 496 377 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2256 2751 3456 3923 4595 4775 4891
Size: 573 520 495 705 467 672 180 116
Cuts at: 4891 1163
Size: 4541
Fragments arranged by size:
4541 705 672 573 520 495 467 180
116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5719 6480 7508 1540
Size: 275 186 3718 761 1028 2301
Fragments arranged by size:
3718 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3421 4553 5181 5296 6330 6415 7486 3421
Size: 1132 628 115 1034 85 1071 4204
Fragments arranged by size:
4204 1132 1071 1034 628 115 85
BsmI GAATG_Cn'
Cuts at: 2385 4986 5136 5780 5873 2385
Size: 2601 150 644 93 4781
Fragments arranged by size:
4781 2601 644 150 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3421 4553 5181 5296 5352 5588 6330
Size: 399 1631 1132 628 115 56 236 742
Cuts at: 6330 6415 7486 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1631 1132 1071 742 628 399 236
115 85 56
BspEI T'CCGG_A
Cuts at: 5121 7932 5121
Size: 2811 5458
BspHI T'CATG_A
Cuts at: 2040 2718 3790 6158 7888 2040
Size: 678 1072 2368 1730 2421
Fragments arranged by size:
2421 2368 1730 1072 678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4362 4747 5017 5624 844
Size: 3518 385 270 607 3489
Fragments arranged by size:
3518 3489 607 385 270
BsrBI CCG'CTC
Cuts at: 1394 1823 6156 1394
Size: 429 4333 3507
Fragments arranged by size:
4333 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6764 6938 65
Size: 1183 5516 174 1396
Fragments arranged by size:
5516 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6913 7100 1700
Size: 110 5103 187 2869
Fragments arranged by size:
5103 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8269
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8269
BssSI C'ACGA_G
Cuts at: 6329 7341 6329
Size: 1012 7257
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3589 794
Size: 2795 5474
BstBI TT'CG_AA
Cuts at: 3949 7073 3949
Size: 3124 5145
BstEII G'GTnAC_C
Cuts at: 1184 2939 4030 1184
Size: 1755 1091 5423
Fragments arranged by size:
5423 1755 1091
BstXI CCAn_nnnn'nTGG
Cuts at: 3258 3656 4138 3258
Size: 398 482 7389
Fragments arranged by size:
7389 482 398
BstYI r'GATC_y
Cuts at: 1972 2227 2932 2982 3115 3261 3586 4439 4819
Size: 255 705 50 133 146 325 853 380
Cuts at: 4819 5599 5966 6351 6368 7820 7906 7918 8264
Size: 780 367 385 17 1452 86 12 346
Cuts at: 8264 1972
Size: 1977
Fragments arranged by size:
1977 1452 853 780 705 385 380 367
346 325 255 146 133 86 50 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4290 513
Size: 552 939 2286 4492
Fragments arranged by size:
4492 2286 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7530
Fragments arranged by size:
7530 683 56
BtsI GCAGTG_nn'
Cuts at: 2199 4161 4256 5785 6579 6599 2199
Size: 1962 95 1529 794 20 3869
Fragments arranged by size:
3869 1962 1529 794 95 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2409 2444 529
Size: 35 1845 35 6354
Fragments arranged by size:
6354 1845 35 35
DraI TTT'AAA
Cuts at: 2683 4890 5573 2683
Size: 2207 683 5379
Fragments arranged by size:
5379 2207 683
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3750 7276 809
Size: 507 2434 3526 1802
Fragments arranged by size:
3526 2434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3340 3669 5716
Size: 54 1425 50 275 18 1510 329 2047
Cuts at: 5716 6605 8234 8
Size: 889 1629 43
Fragments arranged by size:
2047 1629 1510 1425 889 329 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5716 1537
Size: 275 3904 4090
Fragments arranged by size:
4090 3904 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2353 4216 6199 1353
Size: 1000 1863 1983 3423
Fragments arranged by size:
3423 1983 1863 1000
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 6856 7230 7376 387
Size: 1064 138 2843 2424 374 146 1280
Fragments arranged by size:
2843 2424 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4551 5179 5294 4551
Size: 628 115 7526
Fragments arranged by size:
7526 628 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3675 3740 3912 4132 4464 5223 6332
Size: 393 2043 65 172 220 332 759 1109
Cuts at: 6332 6929 7716 7984 1239
Size: 597 787 268 1524
Fragments arranged by size:
2043 1524 1109 787 759 597 393 332
268 220 172 65
EcoO109I rG'GnC_Cy
Cuts at: 2735 3743 4344 4602 2735
Size: 1008 601 258 6402
Fragments arranged by size:
6402 1008 601 258
EcoRI G'AATT_C
Cuts at: 5099 5192 5578 5956 5099
Size: 93 386 378 7412
Fragments arranged by size:
7412 386 378 93
EcoRV GAT'ATC
Cuts at: 2152 3298 2152
Size: 1146 7123
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2708 2951 2708
Size: 243 8026
FspI TGC'GCA
Cuts at: 5135 6775 5135
Size: 1640 6629
FspAI rTGC'GCAy
Cuts at: 5135 5135
Size: 8269
HaeII r_GCGC'y
Cuts at: 1678 7416 1678
Size: 5738 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2188 2220 2823 2855 4562
Size: 32 135 32 616 32 603 32 1707
Cuts at: 4562 4594 6910 6942 6984 7016 1373
Size: 32 2316 32 42 32 2626
Fragments arranged by size:
2626 2316 1707 616 603 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5613 5859 669
Size: 988 281 3675 246 3079
Fragments arranged by size:
3675 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2403 748
Size: 1655 6614
HpaI GTT'AAC
Cuts at: 5859 5859
Size: 8269
KpnI G_GTAC'C
Cuts at: 2137 5594 2137
Size: 3457 4812
MfeI C'AATT_G
Cuts at: 3229 3256 4804 4908 5868 3229
Size: 27 1548 104 960 5630
Fragments arranged by size:
5630 1548 960 104 27
MluI A'CGCG_T
Cuts at: 8145 8145
Size: 8269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2843 2866 3018 3510 4768 7383 7567 1305
Size: 1538 23 152 492 1258 2615 184 2007
Fragments arranged by size:
2615 2007 1538 1258 492 184 152 23
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3342 10
Size: 54 1425 343 1510 4937
Fragments arranged by size:
4937 1510 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 3552 5441 5529 6227 6586 6745
Size: 648 993 1393 1889 88 698 359 159
Cuts at: 6745 518
Size: 2042
Fragments arranged by size:
2042 1889 1393 993 698 648 359 159
88
MspA1I CmG'CkG
Cuts at: 1606 3617 4011 4113 4203 5145 5425 5685 6362
Size: 2011 394 102 90 942 280 260 677
Cuts at: 6362 7510 7755 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 2011 1148 942 677 394 280 260
245 102 90
NcoI C'CATG_G
Cuts at: 513 1065 2004 4290 513
Size: 552 939 2286 4492
Fragments arranged by size:
4492 2286 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8269
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8269
NotI GC'GGCC_GC
Cuts at: 5716 5716
Size: 8269
NspI r_CATG'y
Cuts at: 1132 1229 2037 2260 2418 2849 5627 7172 1132
Size: 97 808 223 158 431 2778 1545 2229
Fragments arranged by size:
2778 2229 1545 808 431 223 158 97
PasI CC'CwG_GG
Cuts at: 1348 5250 1348
Size: 3902 4367
PciI A'CATG_T
Cuts at: 2033 2845 7168 2033
Size: 812 4323 3134
Fragments arranged by size:
4323 3134 812
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3973 1108
Size: 2865 5404
PfoI T'CCnGG_A
Cuts at: 2174 2389 2174
Size: 215 8054
PmeI GTTT'AAAC
Cuts at: 5573 5573
Size: 8269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6338 6370 7851 7883 6338
Size: 32 1481 32 6724
Fragments arranged by size:
6724 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2735 3743 2735
Size: 1008 7261
PshAI GACnn'nnGTC
Cuts at: 1613 5425 1613
Size: 3812 4457
PsiI TTA'TAA
Cuts at: 5839 5839
Size: 8269
PspXI vC'TCGA_Gb
Cuts at: 3420 3998 3420
Size: 578 7691
PstI C_TGCA'G
Cuts at: 830 2148 3594 4118 5347 5434 5621 830
Size: 1318 1446 524 1229 87 187 3478
Fragments arranged by size:
3478 1446 1318 1229 524 187 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8269
PvuII CAG'CTG
Cuts at: 3617 4011 4113 5145 5425 3617
Size: 394 102 1032 280 6461
Fragments arranged by size:
6461 1032 394 280 102
SacI G_AGCT'C
Cuts at: 4553 5181 5296 4553
Size: 628 115 7526
Fragments arranged by size:
7526 628 115
SalI G'TCGA_C
Cuts at: 1936 5611 1936
Size: 3675 4594
SapI GCTCTTCn'nnn_
Cuts at: 4216 4216
Size: 8269
SbfI CC_TGCA'GG
Cuts at: 5621 5621
Size: 8269
ScaI AGT'ACT
Cuts at: 2115 6517 8229 2115
Size: 4402 1712 2155
Fragments arranged by size:
4402 2155 1712
SexAI A'CCwGG_T
Cuts at: 4865 4865
Size: 8269
SfcI C'TryA_G
Cuts at: 826 1045 2144 2562 3092 3590 4114 4227 4527
Size: 219 1099 418 530 498 524 113 300
Cuts at: 4527 5157 5343 5430 5617 6752 7433 7624 826
Size: 630 186 87 187 1135 681 191 1471
Fragments arranged by size:
1471 1135 1099 681 630 530 524 498
418 300 219 191 187 186 113 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8269
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8269
SmaI CCC'GGG
Cuts at: 1872 5596 1872
Size: 3724 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2348 2704 3274 3420 3998 6373
Size: 197 926 405 356 570 146 578 2375
Cuts at: 6373 7274 7536 820
Size: 901 262 1553
Fragments arranged by size:
2375 1553 926 901 578 570 405 356
262 197 146
SnaBI TAC'GTA
Cuts at: 493 6060 493
Size: 5567 2702
SpeI A'CTAG_T
Cuts at: 152 4718 152
Size: 4566 3703
SphI G_CATG'C
Cuts at: 1132 2418 5627 1132
Size: 1286 3209 3774
Fragments arranged by size:
3774 3209 1286
SspI AAT'ATT
Cuts at: 5 52 6193 5
Size: 47 6141 2081
Fragments arranged by size:
6141 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8269
StyI C'CwwG_G
Cuts at: 513 1065 2004 2827 3408 4290 5321 5708 513
Size: 552 939 823 581 882 1031 387 3074
Fragments arranged by size:
3074 1031 939 882 823 581 552 387
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2420 3421 6296 6313 6466 6651 7096
Size: 1255 840 1001 2875 17 153 185 445
Cuts at: 7096 325
Size: 1498
Fragments arranged by size:
2875 1498 1255 1001 840 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2113 4636 4721
Size: 274 80 33 51 1443 136 2523 85
Cuts at: 4721 6515 8227 96
Size: 1794 1712 138
Fragments arranged by size:
2523 1794 1712 1443 274 138 136 85
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 2857 2889 3651 3683 3885 3917 8229
Size: 32 684 32 762 32 202 32 4312
Cuts at: 8229 8261 2141
Size: 32 2149
Fragments arranged by size:
4312 2149 762 684 202 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3644 1316
Size: 2328 5941
XbaI T'CTAG_A
Cuts at: 3823 5605 3823
Size: 1782 6487
XhoI C'TCGA_G
Cuts at: 1943 3420 3998 1943
Size: 1477 578 6214
Fragments arranged by size:
6214 1477 578
XmaI C'CCGG_G
Cuts at: 1870 5594 1870
Size: 3724 4545
XmnI GAAnn'nnTTC
Cuts at: 2811 3060 6398 2811
Size: 249 3338 4682
Fragments arranged by size:
4682 3338 249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6077 276
Size: 53 83 186 936 4543 2468
Fragments arranged by size:
4543 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI