Restriction map for
pFN21AB9486
(Circular) (Six-base) MAPSORT of: pFN21AB9486.seq Check: 8787 from: 1 to: 8368
pFN21AB9486 8368 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6178 278
Size: 53 83 186 936 4642 2468
Fragments arranged by size:
4642 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 2483 5711 1937
Size: 274 272 3228 4594
Fragments arranged by size:
4594 3228 274 272
Acc65I G'GTAC_C
Cuts at: 2643 5689 2643
Size: 3046 5322
AclI AA'CG_TT
Cuts at: 1563 6495 6868 1563
Size: 4932 373 3063
Fragments arranged by size:
4932 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2385 3393 6431 7815 8083 1632
Size: 753 1008 3038 1384 268 1917
Fragments arranged by size:
3038 1917 1384 1008 753 268
AfeI AGC'GCT
Cuts at: 4472 4472
Size: 8368
AflII C'TTAA_G
Cuts at: 820 1017 2339 820
Size: 197 1322 6849
Fragments arranged by size:
6849 1322 197
AflIII A'CryG_T
Cuts at: 1203 2486 3815 7267 8244 1203
Size: 1283 1329 3452 977 1327
Fragments arranged by size:
3452 1329 1327 1283 977
AgeI A'CCGG_T
Cuts at: 7199 7199
Size: 8368
AhdI GACnn_n'nnGTC
Cuts at: 7097 7097
Size: 8368
AleI CACnn'nnGTG
Cuts at: 1166 3637 3660 1166
Size: 2471 23 5874
Fragments arranged by size:
5874 2471 23
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8368
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5179 5211 5179
Size: 32 8336
AlwNI CAG_nnn'CTG
Cuts at: 2326 2557 3097 4348 4766 5543 6111 7683 2326
Size: 231 540 1251 418 777 568 1572 3011
Fragments arranged by size:
3011 1572 1251 777 568 540 418 231
ApaI G_GGCC'C
Cuts at: 2057 3759 4512 2057
Size: 1702 753 5913
Fragments arranged by size:
5913 1702 753
ApaLI G'TGCA_C
Cuts at: 5407 6425 7581 5407
Size: 1018 1156 6194
Fragments arranged by size:
6194 1156 1018
ApoI r'AATT_y
Cuts at: 1493 2528 3455 4079 4536 5677 5903 6055 8164
Size: 1035 927 624 457 1141 226 152 2109
Cuts at: 8164 1493
Size: 1697
Fragments arranged by size:
2109 1697 1141 1035 927 624 457 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6922 160
Size: 872 5890 1606
Fragments arranged by size:
5890 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8368
AvaI C'yCGr_G
Cuts at: 1870 1943 2417 3752 5084 5693 1870
Size: 73 474 1335 1332 609 4545
Fragments arranged by size:
4545 1335 1332 609 474 73
BamHI G'GATC_C
Cuts at: 5557 5698 6065 5557
Size: 141 367 7860
Fragments arranged by size:
7860 367 141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2162 2643 3639 4322 5689
Size: 325 217 627 375 481 996 683 1367
Cuts at: 5689 7144 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1367 996 683 627 481 375
325 217
BanII G_rGCy'C
Cuts at: 1391 2057 2840 3498 3753 3759 3961 4512 4773
Size: 666 783 658 255 6 202 551 261
Cuts at: 4773 5066 5687 1391
Size: 293 621 4072
Fragments arranged by size:
4072 783 666 658 621 551 293 261
255 202 6
BbeI G_GCGC'C
Cuts at: 2166 4326 2166
Size: 2160 6208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3420 928
Size: 971 1521 5876
Fragments arranged by size:
5876 1521 971
BbvCI CC'TCA_GC
Cuts at: 2550 3461 5601 2550
Size: 911 2140 5317
Fragments arranged by size:
5317 2140 911
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5180 5214 5328 5362 6559 6593 1903
Size: 34 3243 34 114 34 1197 34 3678
Fragments arranged by size:
3678 3243 1197 114 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6260 7470 6260
Size: 1210 7158
BclI T'GATC_A
Cuts at: 1548 3072 3154 4180 1548
Size: 1524 82 1026 5736
Fragments arranged by size:
5736 1524 1026 82
BfrBI ATG'CAT
Cuts at: 2708 2708
Size: 8368
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2039 6979 136
Size: 107 122 71 631 605 367 4940 1525
Fragments arranged by size:
4940 1525 631 605 367 122 107 71
BglII A'GATC_T
Cuts at: 8363 8363
Size: 8368
BlpI GC'TnA_GC
Cuts at: 5785 5785
Size: 8368
Bme1580I G_kGCm'C
Cuts at: 1790 2057 3324 3759 4512 5411 6429 7585 1790
Size: 267 1267 435 753 899 1018 1156 2573
Fragments arranged by size:
2573 1267 1156 1018 899 753 435 267
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2087 6099 7057
Size: 416 53 83 399 24 670 4012 958
Cuts at: 7057 442
Size: 1753
Fragments arranged by size:
4012 1753 958 670 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8368
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 3081 4345 4435 4657 4869 7028 1239
Size: 845 997 1264 90 222 212 2159 2579
Fragments arranged by size:
2579 2159 1264 997 845 222 212 90
Bpu10I CC'TnA_GC
Cuts at: 2140 2550 3461 5115 5601 2140
Size: 410 911 1654 486 4907
Fragments arranged by size:
4907 1654 911 486 410
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3434 3727 4707 5461 6493 7358 7656 3434
Size: 293 980 754 1032 865 298 4146
Fragments arranged by size:
4146 1032 980 865 754 298 293
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3659 5010 5321 7031 882
Size: 617 2160 1351 311 1710 2219
Fragments arranged by size:
2219 2160 1710 1351 617 311
BsaAI yAC'GTr
Cuts at: 493 1204 2487 2605 6159 493
Size: 711 1283 118 3554 2702
Fragments arranged by size:
3554 2702 1283 711 118
BsaBI GATnn'nnATC
Cuts at: 8362 8362
Size: 8368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 4323 6175 6557
Size: 53 83 186 936 630 2160 1852 382
Cuts at: 6557 7192 275
Size: 635 1451
Fragments arranged by size:
2160 1852 1451 936 635 630 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3401 3523 4300 5503 6801 7199 7473 7620 8031
Size: 122 777 1203 1298 398 274 147 411
Cuts at: 8031 3401
Size: 3738
Fragments arranged by size:
3738 1298 1203 777 411 398 274 147
122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2450 2480 2564 2594 4775
Size: 30 165 30 1047 30 84 30 2181
Cuts at: 4775 4805 5179 5209 5222 5252 1178
Size: 30 374 30 13 30 4294
Fragments arranged by size:
4294 2181 1047 374 165 84 30 30
30 30 30 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2455 2458 2642 3840 5042 1186
Size: 1269 3 184 1198 1202 4512
Fragments arranged by size:
4512 1269 1202 1198 184 3
BseYI C'CCAG_C
Cuts at: 4448 5350 7571 8067 4448
Size: 902 2221 496 4749
Fragments arranged by size:
4749 2221 902 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2279 2312 2444 3990 4667 5260 1163
Size: 573 543 33 132 1546 677 593 4271
Fragments arranged by size:
4271 1546 677 593 573 543 132 33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2130 2270 5818 6579 7607 1540
Size: 275 186 129 140 3548 761 1028 2301
Fragments arranged by size:
3548 2301 1028 761 275 186 140 129
BsiHKAI G_wGCw'C
Cuts at: 2840 3248 3753 4661 4940 5411 6429 6514 7585
Size: 408 505 908 279 471 1018 85 1071
Cuts at: 7585 2840
Size: 3623
Fragments arranged by size:
3623 1071 1018 908 505 471 408 279
85
BsmI GAATG_Cn'
Cuts at: 2284 4918 5879 5972 2284
Size: 2634 961 93 4680
Fragments arranged by size:
4680 2634 961 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4514 4669 4514
Size: 155 8213
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2840 3248 3324 3498 3753 3759
Size: 399 267 783 408 76 174 255 6
Cuts at: 3759 3961 4512 4661 4773 4940 5066 5411 5687
Size: 202 551 149 112 167 126 345 276
Cuts at: 5687 6429 6514 7585 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 783 742 551 408 399 345
276 267 255 202 174 167 149 126
112 85 76 6
BspEI T'CCGG_A
Cuts at: 3523 8031 3523
Size: 4508 3860
BspHI T'CATG_A
Cuts at: 3310 4183 4593 6257 7987 3310
Size: 873 410 1664 1730 3691
Fragments arranged by size:
3691 1730 1664 873 410
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4342 4633 5339 5723 844
Size: 3498 291 706 384 3489
Fragments arranged by size:
3498 3489 706 384 291
BsrBI CCG'CTC
Cuts at: 1394 1823 6255 1394
Size: 429 4432 3507
Fragments arranged by size:
4432 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2435 3940 4098 5390 6863 7037 65
Size: 1183 1187 1505 158 1292 1473 174 1396
Fragments arranged by size:
1505 1473 1396 1292 1187 1183 174 158
BsrFI r'CCGG_y
Cuts at: 1700 1810 5160 5466 7012 7199 1700
Size: 110 3350 306 1546 187 2869
Fragments arranged by size:
3350 2869 1546 306 187 110
BsrGI T'GTAC_A
Cuts at: 96 2610 5364 96
Size: 2514 2754 3100
Fragments arranged by size:
3100 2754 2514
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8368
BssSI C'ACGA_G
Cuts at: 2897 6428 7440 2897
Size: 3531 1012 3825
Fragments arranged by size:
3825 3531 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5652 794
Size: 4858 3510
BstBI TT'CG_AA
Cuts at: 4378 7172 4378
Size: 2794 5574
BstEII G'GTnAC_C
Cuts at: 1184 2591 1184
Size: 1407 6961
BstXI CCAn_nnnn'nTGG
Cuts at: 2384 4821 2384
Size: 2437 5931
BstYI r'GATC_y
Cuts at: 1972 2634 4869 5557 5698 6065 6450 6467 7919
Size: 662 2235 688 141 367 385 17 1452
Cuts at: 7919 8005 8017 8363 1972
Size: 86 12 346 1977
Fragments arranged by size:
2235 1977 1452 688 662 385 367 346
141 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3471 3471
Size: 8368
BtgI C'CryG_G
Cuts at: 513 1065 2004 2133 2647 3769 3994 5396 513
Size: 552 939 129 514 1122 225 1402 3485
Fragments arranged by size:
3485 1402 1122 939 552 514 225 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3289 487
Size: 683 56 2063 5566
Fragments arranged by size:
5566 2063 683 56
BtsI GCAGTG_nn'
Cuts at: 3847 4750 4917 5411 5884 6678 6698 3847
Size: 903 167 494 473 794 20 5517
Fragments arranged by size:
5517 903 794 494 473 167 20
ClaI AT'CG_AT
Cuts at: 1554 4045 1554
Size: 2491 5877
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5005 5040 529
Size: 35 4441 35 3857
Fragments arranged by size:
4441 3857 35 35
DraI TTT'AAA
Cuts at: 2992 5672 2992
Size: 2680 5688
DraIII CAC_nnn'GTG
Cuts at: 3564 3564
Size: 8368
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7375 809
Size: 507 6059 1802
Fragments arranged by size:
6059 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2665 3772
Size: 54 1425 50 275 18 258 577 1107
Cuts at: 3772 5815 6704 8333 8
Size: 2043 889 1629 43
Fragments arranged by size:
2043 1629 1425 1107 889 577 275 258
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5815 1537
Size: 275 4003 4090
Fragments arranged by size:
4090 4003 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4029 5219 5319 6298 1353
Size: 2676 1190 100 979 3423
Fragments arranged by size:
3423 2676 1190 979 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3772 6955 7329 7475 387
Size: 1064 138 2183 3183 374 146 1280
Fragments arranged by size:
3183 2183 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2838 3751 2838
Size: 913 7455
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2385 3081 3393 4345 4435 4657
Size: 393 452 301 696 312 952 90 222
Cuts at: 4657 4869 6431 7028 7815 8083 1239
Size: 212 1562 597 787 268 1524
Fragments arranged by size:
1562 1524 952 787 696 597 452 393
312 301 268 222 212 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2815 5057 2815
Size: 2242 6126
EcoO109I rG'GnC_Cy
Cuts at: 2065 2878 3756 4508 2065
Size: 813 878 752 5925
Fragments arranged by size:
5925 878 813 752
EcoRI G'AATT_C
Cuts at: 2528 5677 6055 2528
Size: 3149 378 4841
Fragments arranged by size:
4841 3149 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4909 4909
Size: 8368
FseI GG_CCGG'CC
Cuts at: 5470 5470
Size: 8368
FspI TGC'GCA
Cuts at: 6874 6874
Size: 8368
HaeII r_GCGC'y
Cuts at: 1678 2099 2166 4326 4474 7515 1678
Size: 421 67 2160 148 3041 2531
Fragments arranged by size:
3041 2531 2160 421 148 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2450 2482 2716 2748 3057
Size: 32 135 32 878 32 234 32 309
Cuts at: 3057 3064 3089 3096 3139 3171 5542 5546 5574
Size: 7 25 7 43 32 2371 4 28
Cuts at: 5574 5578 7009 7041 7083 7115 1373
Size: 4 1431 32 42 32 2626
Fragments arranged by size:
2626 2371 1431 878 309 234 135 43
42 32 32 32 32 32 32 32
28 25 7 7 4 4
HincII GTy'rAC
Cuts at: 669 1657 1938 5712 5958 669
Size: 988 281 3774 246 3079
Fragments arranged by size:
3774 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8368
HpaI GTT'AAC
Cuts at: 5958 5958
Size: 8368
KasI G'GCGC_C
Cuts at: 2162 4322 2162
Size: 2160 6208
KpnI G_GTAC'C
Cuts at: 2647 5693 2647
Size: 3046 5322
MfeI C'AATT_G
Cuts at: 5967 5967
Size: 8368
MluI A'CGCG_T
Cuts at: 8244 8244
Size: 8368
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7482 7666 1305
Size: 6177 184 2007
Fragments arranged by size:
6177 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 10
Size: 54 1425 343 258 6288
Fragments arranged by size:
6288 1425 343 258 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2763 2966 3020 3156 3637 3660 5395
Size: 648 1597 203 54 136 481 23 1735
Cuts at: 5395 6326 6685 6844 518
Size: 931 359 159 2042
Fragments arranged by size:
2042 1735 1597 931 648 481 359 203
159 136 54 23
MspA1I CmG'CkG
Cuts at: 1606 2104 2135 2160 2169 2641 2861 3913 4319
Size: 498 31 25 9 472 220 1052 406
Cuts at: 4319 5784 6461 7609 7854 1606
Size: 1465 677 1148 245 2120
Fragments arranged by size:
2120 1465 1148 1052 677 498 472 406
245 220 31 25 9
NaeI GCC'GGC
Cuts at: 5162 5468 5162
Size: 306 8062
NarI GG'CG_CC
Cuts at: 2163 4323 2163
Size: 2160 6208
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6877
Fragments arranged by size:
6877 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8368
NgoMIV G'CCGG_C
Cuts at: 5160 5466 5160
Size: 306 8062
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8368
NotI GC'GGCC_GC
Cuts at: 5815 5815
Size: 8368
NsiI A_TGCA'T
Cuts at: 2710 2710
Size: 8368
NspI r_CATG'y
Cuts at: 1132 1229 3819 4833 5726 7271 1132
Size: 97 2590 1014 893 1545 2229
Fragments arranged by size:
2590 2229 1545 1014 893 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8368
PciI A'CATG_T
Cuts at: 3815 7267 3815
Size: 3452 4916
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8368
PfoI T'CCnGG_A
Cuts at: 3809 3837 4341 3809
Size: 28 504 7836
Fragments arranged by size:
7836 504 28
PmeI GTTT'AAAC
Cuts at: 5672 5672
Size: 8368
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5179 5211 6437 6469 7950 7982 5179
Size: 32 1226 32 1481 32 5565
Fragments arranged by size:
5565 1481 1226 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4526 5002 1613
Size: 2913 476 4979
Fragments arranged by size:
4979 2913 476
PsiI TTA'TAA
Cuts at: 4257 5938 4257
Size: 1681 6687
PspOMI G'GGCC_C
Cuts at: 2053 3755 4508 2053
Size: 1702 753 5913
Fragments arranged by size:
5913 1702 753
PstI C_TGCA'G
Cuts at: 830 3365 3437 4353 4745 5124 5720 830
Size: 2535 72 916 392 379 596 3478
Fragments arranged by size:
3478 2535 916 596 392 379 72
PvuI CG_AT'CG
Cuts at: 2001 2270 2001
Size: 269 8099
PvuII CAG'CTG
Cuts at: 2104 2861 3913 4319 2104
Size: 757 1052 406 6153
Fragments arranged by size:
6153 1052 757 406
SacI G_AGCT'C
Cuts at: 2840 3753 2840
Size: 913 7455
SacII CC_GC'GG
Cuts at: 2136 2136
Size: 8368
SalI G'TCGA_C
Cuts at: 1936 5710 1936
Size: 3774 4594
SbfI CC_TGCA'GG
Cuts at: 5720 5720
Size: 8368
ScaI AGT'ACT
Cuts at: 6616 8328 6616
Size: 1712 6656
SfcI C'TryA_G
Cuts at: 826 1045 2575 3361 3433 4349 4741 5120 5165
Size: 219 1530 786 72 916 392 379 45
Cuts at: 5165 5716 6851 7532 7723 826
Size: 551 1135 681 191 1471
Fragments arranged by size:
1530 1471 1135 916 786 681 551 392
379 219 191 72 45
SfoI GGC'GCC
Cuts at: 2164 4324 2164
Size: 2160 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8368
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8368
SmaI CCC'GGG
Cuts at: 1872 5695 1872
Size: 3823 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2339 3449 3706 4722 5476 6472
Size: 197 926 396 1110 257 1016 754 996
Cuts at: 6472 7373 7635 820
Size: 901 262 1553
Fragments arranged by size:
1553 1110 1016 996 926 901 754 396
262 257 197
SnaBI TAC'GTA
Cuts at: 493 6159 493
Size: 5666 2702
SpeI A'CTAG_T
Cuts at: 152 4168 152
Size: 4016 4352
SphI G_CATG'C
Cuts at: 1132 5726 1132
Size: 4594 3774
SspI AAT'ATT
Cuts at: 5 52 3922 4295 5299 6292 5
Size: 47 3870 373 1004 993 2081
Fragments arranged by size:
3870 2081 1004 993 373 47
StuI AGG'CCT
Cuts at: 1517 4263 4841 1517
Size: 2746 578 5044
Fragments arranged by size:
5044 2746 578
StyI C'CwwG_G
Cuts at: 513 1065 2004 2091 3604 4587 4993 5807 513
Size: 552 939 87 1513 983 406 814 3074
Fragments arranged by size:
3074 1513 983 939 814 552 406 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2116 4957 5204 6395 6412 6565 6750
Size: 1255 536 2841 247 1191 17 153 185
Cuts at: 6750 7195 325
Size: 445 1498
Fragments arranged by size:
2841 1498 1255 1191 536 445 247 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2610 4056 4826
Size: 274 80 33 51 1443 633 1446 770
Cuts at: 4826 5364 6614 8326 96
Size: 538 1250 1712 138
Fragments arranged by size:
1712 1446 1443 1250 770 633 538 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3685 3717 3805 3837 4801 4833 5019 5051 5222
Size: 32 88 32 964 32 186 32 171
Cuts at: 5222 5254 8328 8360 3685
Size: 32 3074 32 3693
Fragments arranged by size:
3693 3074 964 186 171 88 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5440 1316
Size: 4124 4244
XbaI T'CTAG_A
Cuts at: 5704 5704
Size: 8368
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2645 4566 2645
Size: 1921 6447
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8368
XmaI C'CCGG_G
Cuts at: 1870 5693 1870
Size: 3823 4545
XmnI GAAnn'nnTTC
Cuts at: 4892 5252 6497 4892
Size: 360 1245 6763
Fragments arranged by size:
6763 1245 360
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6176 276
Size: 53 83 186 936 4642 2468
Fragments arranged by size:
4642 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BplI BsiWI BstZ17I EcoRV
FspAI NruI PacI PmlI PpuMI PspXI PsrI RsrII
SanDI SapI SexAI SfiI SrfI SwaI