Restriction map for
pFN21AB9484
(Circular) (Six-base) MAPSORT of: pFN21AB9484.seq Check: 9441 from: 1 to: 10840
pFN21AB9484 10840 bp
With 182 enzymes: SgfI *
October 20, 2009 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6074 7414 7794 7894 8097 6074
Size: 1340 380 100 203 8817
Fragments arranged by size:
8817 1340 380 203 100
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5550 5968 6579 7434
Size: 53 83 186 936 4014 418 611 855
Cuts at: 7434 8650 278
Size: 1216 2468
Fragments arranged by size:
4014 2468 1216 936 855 611 418 186
83 53
AccI GT'mk_AC
Cuts at: 1937 8183 1937
Size: 6246 4594
Acc65I G'GTAC_C
Cuts at: 8161 8161
Size: 10840
AclI AA'CG_TT
Cuts at: 1563 8967 9340 1563
Size: 7404 373 3063
Fragments arranged by size:
7404 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4108 4217 4874 5629 7300 7301 8903 10287
Size: 2476 109 657 755 1671 1 1602 1384
Cuts at: 10287 10555 1632
Size: 268 1917
Fragments arranged by size:
2476 1917 1671 1602 1384 755 657 268
109 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10643
AflIII A'CryG_T
Cuts at: 1203 2190 3437 3720 5924 9739 10716 1203
Size: 987 1247 283 2204 3815 977 1327
Fragments arranged by size:
3815 2204 1327 1247 987 977 283
AgeI A'CCGG_T
Cuts at: 9671 9671
Size: 10840
AhdI GACnn_n'nnGTC
Cuts at: 5476 7075 7435 9569 5476
Size: 1599 360 2134 6747
Fragments arranged by size:
6747 2134 1599 360
AleI CACnn'nnGTG
Cuts at: 1166 2492 5399 1166
Size: 1326 2907 6607
Fragments arranged by size:
6607 2907 1326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2726 3372 4009 6584 6981 1184
Size: 1542 646 637 2575 397 5043
Fragments arranged by size:
5043 2575 1542 646 637 397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 6989 7021 2438
Size: 32 4519 32 6257
Fragments arranged by size:
6257 4519 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2778 2899 3930 4060 4380 4879 4900 4993 5446
Size: 121 1031 130 320 499 21 93 453
Cuts at: 5446 6226 6592 6703 7399 7717 8583 10155 2778
Size: 780 366 111 696 318 866 1572 3463
Fragments arranged by size:
3463 1572 1031 866 780 696 499 453
366 320 318 130 121 111 93 21
ApaI G_GGCC'C
Cuts at: 2030 3383 4667 4907 5592 5671 5869 6691 6909
Size: 1353 1284 240 685 79 198 822 218
Cuts at: 6909 8012 2030
Size: 1103 4858
Fragments arranged by size:
4858 1353 1284 1103 822 685 240 218
198 79
ApaLI G'TGCA_C
Cuts at: 3749 5621 8897 10053 3749
Size: 1872 3276 1156 4536
Fragments arranged by size:
4536 3276 1872 1156
ApoI r'AATT_y
Cuts at: 1493 5539 6200 8149 8375 8527 10636 1493
Size: 4046 661 1949 226 152 2109 1697
Fragments arranged by size:
4046 2109 1949 1697 661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 9394 160
Size: 872 8362 1606
Fragments arranged by size:
8362 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10840
AvaI C'yCGr_G
Cuts at: 1870 1943 2023 2247 2474 2843 2983 3041 3132
Size: 73 80 224 227 369 140 58 91
Cuts at: 3132 3410 3487 3569 3981 4347 4613 5258 5459
Size: 278 77 82 412 366 266 645 201
Cuts at: 5459 5664 5862 6249 6500 6624 6824 6886 7461
Size: 205 198 387 251 124 200 62 575
Cuts at: 7461 7504 7535 8165 1870
Size: 43 31 630 4545
Fragments arranged by size:
4545 645 630 575 412 387 369 366
278 266 251 227 224 205 201 200
198 140 124 91 82 80 77 73
62 58 43 31
BamHI G'GATC_C
Cuts at: 8170 8537 8170
Size: 367 10473
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2543 2623 2837 2870 2969
Size: 325 217 627 756 80 214 33 99
Cuts at: 2969 3018 3064 4412 4562 5009 5125 5137 5650
Size: 49 46 1348 150 447 116 12 513
Cuts at: 5650 5697 6092 6116 6366 6393 6401 6452 6542
Size: 47 395 24 250 27 8 51 90
Cuts at: 6542 6805 6866 7211 7361 7782 7946 8076 8161
Size: 263 61 345 150 421 164 130 85
Cuts at: 8161 9616 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1348 756 627 513 447 421
395 345 325 263 250 217 214 164
150 150 130 116 99 90 85 80
61 51 49 47 46 33 27 24
12 8
BanII G_rGCy'C
Cuts at: 1391 2030 2559 2606 3198 3383 3637 4230 4667
Size: 639 529 47 592 185 254 593 437
Cuts at: 4667 4907 4977 5259 5265 5381 5592 5671 5869
Size: 240 70 282 6 116 211 79 198
Cuts at: 5869 6691 6776 6909 7811 7975 8012 8159 1391
Size: 822 85 133 902 164 37 147 4072
Fragments arranged by size:
4072 902 822 639 593 592 529 437
282 254 240 211 198 185 164 147
133 116 85 79 70 47 37 6
BbeI G_GCGC'C
Cuts at: 2547 2627 2841 2874 2973 3022 3068 4566 5013
Size: 80 214 33 99 49 46 1498 447
Cuts at: 5013 5701 6096 6120 6397 6546 6809 7950 2547
Size: 688 395 24 277 149 263 1141 5437
Fragments arranged by size:
5437 1498 1141 688 447 395 277 263
214 149 99 80 49 46 33 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2923 4862 5360 7058 928
Size: 971 1024 1939 498 1698 4710
Fragments arranged by size:
4710 1939 1698 1024 971 498
BbvCI CC'TCA_GC
Cuts at: 3787 3787
Size: 10840
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2122 2156 3168 3202 6815 6849 9031
Size: 34 185 34 1012 34 3613 34 2182
Cuts at: 9031 9065 1903
Size: 34 3678
Fragments arranged by size:
3678 3613 2182 1012 185 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7591 8732 9942 7591
Size: 1141 1210 8489
Fragments arranged by size:
8489 1210 1141
BclI T'GATC_A
Cuts at: 1548 2283 1548
Size: 735 10105
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3062 4365 4530
Size: 107 122 71 631 605 1390 1303 165
Cuts at: 4530 4671 4875 5648 6081 6223 6738 7026 9451
Size: 141 204 773 433 142 515 288 2425
Cuts at: 9451 136
Size: 1525
Fragments arranged by size:
2425 1525 1390 1303 773 631 605 515
433 288 204 165 142 141 122 107
71
BglII A'GATC_T
Cuts at: 10835 10835
Size: 10840
BlpI GC'TnA_GC
Cuts at: 2714 4756 8257 2714
Size: 2042 3501 5297
Fragments arranged by size:
5297 3501 2042
Bme1580I G_kGCm'C
Cuts at: 1790 2030 2612 2822 3191 3295 3383 3671 3720
Size: 240 582 210 369 104 88 288 49
Cuts at: 3720 3753 4306 4660 4667 4907 5130 5592 5625
Size: 33 553 354 7 240 223 462 33
Cuts at: 5625 5653 5671 5869 5961 6404 6691 6734 6851
Size: 28 18 198 92 443 287 43 117
Cuts at: 6851 6869 6909 7216 7785 7842 8012 8901 10057
Size: 18 40 307 569 57 170 889 1156
Cuts at: 10057 1790
Size: 2573
Fragments arranged by size:
2573 1156 889 582 569 553 462 443
369 354 307 288 287 240 240 223
210 198 170 117 104 92 88 57
49 43 40 33 33 28 18 18
7
BmgBI CAC'GTC
Cuts at: 1318 3438 5659 5787 5927 7437 7594 1318
Size: 2120 2221 128 140 1510 157 4564
Fragments arranged by size:
4564 2221 2120 1510 157 140 128
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 4368 5871
Size: 416 53 83 399 24 923 2028 1503
Cuts at: 5871 6052 6441 8132 8571 9529 442
Size: 181 389 1691 439 958 1753
Fragments arranged by size:
2028 1753 1691 1503 958 923 439 416
399 389 181 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10840
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2321 4771 6160 2321
Size: 2450 1389 7001
Fragments arranged by size:
7001 2450 1389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2395 2462 2475 3569 3941 5438 5444
Size: 893 263 67 13 1094 372 1497 6
Cuts at: 5444 6233 6395 6545 6614 6725 6962 7016 7124
Size: 789 162 150 69 111 237 54 108
Cuts at: 7124 7211 7747 8114 9500 1239
Size: 87 536 367 1386 2579
Fragments arranged by size:
2579 1497 1386 1094 893 789 536 372
367 263 237 162 150 111 108 87
69 67 54 13 6
Bpu10I CC'TnA_GC
Cuts at: 3787 4982 6299 3787
Size: 1195 1317 8328
Fragments arranged by size:
8328 1317 1195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 8965 9830 10128 8965
Size: 865 298 9677
Fragments arranged by size:
9677 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2292 3143 3174 3644 3797 6416 7189
Size: 617 793 851 31 470 153 2619 773
Cuts at: 7189 7381 9503 882
Size: 192 2122 2219
Fragments arranged by size:
2619 2219 2122 851 793 773 617 470
192 153 31
BsaAI yAC'GTr
Cuts at: 493 1204 2193 3309 3723 3748 3781 4234 5737
Size: 711 989 1116 414 25 33 453 1503
Cuts at: 5737 7297 7414 8631 493
Size: 1560 117 1217 2702
Fragments arranged by size:
2702 1560 1503 1217 1116 989 711 453
414 117 33 25
BsaBI GATnn'nnATC
Cuts at: 10834 10834
Size: 10840
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2504 2544 2582 2624
Size: 53 83 186 936 971 40 38 42
Cuts at: 2624 2643 2838 2871 2970 3019 3065 3522 4310
Size: 19 195 33 99 49 46 457 788
Cuts at: 4310 4563 5010 5165 5357 5412 5430 5547 5698
Size: 253 447 155 192 55 18 117 151
Cuts at: 5698 5965 6093 6117 6394 6543 6576 6806 7431
Size: 267 128 24 277 149 33 230 625
Cuts at: 7431 7947 8100 8647 9029 9664 275
Size: 516 153 547 382 635 1451
Fragments arranged by size:
1451 971 936 788 635 625 547 516
457 447 382 277 267 253 230 195
192 186 155 153 151 149 128 117
99 83 55 53 49 46 42 40
38 33 33 24 19 18
BsaWI w'CCGG_w
Cuts at: 4016 5535 5562 9273 9671 9945 10092 10503 4016
Size: 1519 27 3711 398 274 147 411 4353
Fragments arranged by size:
4353 3711 1519 411 398 274 147 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2180 2210 2438 2468 2732
Size: 30 165 30 777 30 228 30 264
Cuts at: 2732 2762 3804 3834 4519 4549 5041 5071 6081
Size: 30 1042 30 685 30 492 30 1010
Cuts at: 6081 6111 6917 6947 6989 7019 7793 7823 1178
Size: 30 806 30 42 30 774 30 4195
Fragments arranged by size:
4195 1042 1010 806 777 774 685 492
264 228 165 42 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2138 2141 2174 2459 3131 3989 4760 4967
Size: 952 3 33 285 672 858 771 207
Cuts at: 4967 7924 1186
Size: 2957 4102
Fragments arranged by size:
4102 2957 952 858 771 672 285 207
33 3
BseYI C'CCAG_C
Cuts at: 2612 3365 3693 3728 4423 4928 5027 5153 5948
Size: 753 328 35 695 505 99 126 795
Cuts at: 5948 5982 7022 7812 7942 8011 10043 10539 2612
Size: 34 1040 790 130 69 2032 496 2913
Fragments arranged by size:
2913 2032 1040 795 790 753 695 505
496 328 130 126 99 69 35 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2204 2346 2707 3437 3728 3742 3761
Size: 573 468 142 361 730 291 14 19
Cuts at: 3761 5133 6314 6941 7441 8069 1163
Size: 1372 1181 627 500 628 3934
Fragments arranged by size:
3934 1372 1181 730 628 627 573 500
468 361 291 142 19 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2014 2039 2793 3122 3256 4654
Size: 275 186 13 25 754 329 134 1398
Cuts at: 4654 5723 6742 7204 8290 9051 10079 1540
Size: 1069 1019 462 1086 761 1028 2301
Fragments arranged by size:
2301 1398 1086 1069 1028 1019 761 754
462 329 275 186 134 25 13
BsiHKAI G_wGCw'C
Cuts at: 2145 3488 3753 3845 3879 4442 4604 5519 5625
Size: 1343 265 92 34 563 162 915 106
Cuts at: 5625 5736 6838 7413 7725 8901 8986 10057 2145
Size: 111 1102 575 312 1176 85 1071 2928
Fragments arranged by size:
2928 1343 1176 1102 1071 915 575 563
312 265 162 111 106 92 85 34
BsmI GAATG_Cn'
Cuts at: 2532 2669 3476 3683 8351 8444 2532
Size: 137 807 207 4668 93 4928
Fragments arranged by size:
4928 4668 807 207 137 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2145 2559 2606 2612 2822 3191
Size: 399 240 115 414 47 6 210 369
Cuts at: 3191 3198 3295 3383 3488 3637 3671 3720 3753
Size: 7 97 88 105 149 34 49 33
Cuts at: 3753 3845 3879 4230 4306 4442 4604 4660 4667
Size: 92 34 351 76 136 162 56 7
Cuts at: 4667 4907 4977 5130 5259 5265 5381 5519 5592
Size: 240 70 153 129 6 116 138 73
Cuts at: 5592 5625 5653 5671 5736 5869 5961 6404 6691
Size: 33 28 18 65 133 92 443 287
Cuts at: 6691 6734 6776 6838 6851 6869 6909 7216 7413
Size: 43 42 62 13 18 40 307 197
Cuts at: 7413 7725 7785 7811 7842 7975 8012 8159 8901
Size: 312 60 26 31 133 37 147 742
Cuts at: 8901 8986 10057 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 414 399 369 351
312 307 287 240 240 210 197 162
153 149 147 138 136 133 133 129
116 115 105 97 92 92 88 85
76 73 70 65 62 60 56 49
47 43 42 40 37 34 34 33
33 31 28 26 18 18 13 7
7 6 6
BspEI T'CCGG_A
Cuts at: 4016 5535 5562 10503 4016
Size: 1519 27 4941 4353
Fragments arranged by size:
4941 4353 1519 27
BspHI T'CATG_A
Cuts at: 8729 10459 8729
Size: 1730 9110
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2768 2837 6041 6074 7414 7436 7794 7894
Size: 1924 69 3204 33 1340 22 358 100
Cuts at: 7894 8097 8195 844
Size: 203 98 3489
Fragments arranged by size:
3489 3204 1924 1340 358 203 100 98
69 33 22
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 2116 5220 6144 6529 7291 7747
Size: 429 227 66 3104 924 385 762 456
Cuts at: 7747 8727 1394
Size: 980 3507
Fragments arranged by size:
3507 3104 980 924 762 456 429 385
227 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 5796 6721 7918 9335 9509 65
Size: 1183 4548 925 1197 1417 174 1396
Fragments arranged by size:
4548 1417 1396 1197 1183 925 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2009 2013 2084 3201 3345 4065 4996
Size: 110 199 4 71 1117 144 720 931
Cuts at: 4996 5700 6127 6322 6346 6545 7928 8073 9484
Size: 704 427 195 24 199 1383 145 1411
Cuts at: 9484 9671 1700
Size: 187 2869
Fragments arranged by size:
2869 1411 1383 1117 931 720 704 427
199 199 195 187 145 144 110 71
24 4
BsrGI T'GTAC_A
Cuts at: 96 4535 6234 96
Size: 4439 1699 4702
Fragments arranged by size:
4702 4439 1699
BssHII G'CGCG_C
Cuts at: 1925 2104 2106 2783 3590 1925
Size: 179 2 677 807 9175
Fragments arranged by size:
9175 807 677 179 2
BssSI C'ACGA_G
Cuts at: 2809 4603 7014 8900 9912 2809
Size: 1794 2411 1886 1012 3737
Fragments arranged by size:
3737 2411 1886 1794 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2664 3154 3540 3930 6873 7417 8083 794
Size: 1870 490 386 390 2943 544 666 3551
Fragments arranged by size:
3551 2943 1870 666 544 490 390 386
BstBI TT'CG_AA
Cuts at: 9644 9644
Size: 10840
BstEII G'GTnAC_C
Cuts at: 1184 4171 7448 1184
Size: 2987 3277 4576
Fragments arranged by size:
4576 3277 2987
BstXI CCAn_nnnn'nTGG
Cuts at: 2376 2620 5161 7372 7957 2376
Size: 244 2541 2211 585 5259
Fragments arranged by size:
5259 2541 2211 585 244
BstYI r'GATC_y
Cuts at: 1972 2163 2343 3579 5682 8170 8537 8922 8939
Size: 191 180 1236 2103 2488 367 385 17
Cuts at: 8939 10391 10477 10489 10835 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2488 2103 1977 1452 1236 385 367 346
191 180 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4466 6219 6551 7468 4466
Size: 1753 332 917 7838
Fragments arranged by size:
7838 1753 917 332
BtgI C'CryG_G
Cuts at: 513 1065 2004 2719 2833 3229 3555 4396 4668
Size: 552 939 715 114 396 326 841 272
Cuts at: 4668 5133 5811 5907 6022 6516 6679 6796 7740
Size: 465 678 96 115 494 163 117 944
Cuts at: 7740 7818 8004 513
Size: 78 186 3349
Fragments arranged by size:
3349 944 939 841 715 678 552 494
465 396 326 272 186 163 117 115
114 96 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4846 5052 5220 5817 487
Size: 683 56 3620 206 168 597 5510
Fragments arranged by size:
5510 3620 683 597 206 168 56
BtsI GCAGTG_nn'
Cuts at: 2690 3425 3626 3860 3931 4646 4991 5082 5826
Size: 735 201 234 71 715 345 91 744
Cuts at: 5826 5940 7085 7555 7664 8015 8356 9150 9170
Size: 114 1145 470 109 351 341 794 20
Cuts at: 9170 2690
Size: 4360
Fragments arranged by size:
4360 1145 794 744 735 715 470 351
345 341 234 201 114 109 91 71
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10840
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4795 4830 529
Size: 35 4231 35 6539
Fragments arranged by size:
6539 4231 35 35
DraI TTT'AAA
Cuts at: 8144 8144
Size: 10840
DraIII CAC_nnn'GTG
Cuts at: 4188 5109 4188
Size: 921 9919
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3086 9847 809
Size: 507 1770 6761 1802
Fragments arranged by size:
6761 1802 1770 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2011 2036
Size: 54 1425 50 275 18 177 4 25
Cuts at: 2036 2061 2378 2790 3163 3232 4522 4651 5283
Size: 25 317 412 373 69 1290 129 632
Cuts at: 5283 5720 5814 6286 6739 7201 7767 8287 9176
Size: 437 94 472 453 462 566 520 889
Cuts at: 9176 10805 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1290 889 632 566 520 472
462 453 437 412 373 317 275 177
129 94 69 54 50 43 25 25
18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2011 2036 2790 4651 5720 6739 7201
Size: 275 199 25 754 1861 1069 1019 462
Cuts at: 7201 8287 1537
Size: 1086 4090
Fragments arranged by size:
4090 1861 1086 1069 1019 754 462 275
199 25
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 5537 6764 8770 1353
Size: 1241 2943 1227 2006 3423
Fragments arranged by size:
3423 2943 2006 1241 1227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2696 5049 5158 7633 9427 9801
Size: 1064 138 1107 2353 109 2475 1794 374
Cuts at: 9801 9947 387
Size: 146 1280
Fragments arranged by size:
2475 2353 1794 1280 1107 1064 374 146
138 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2395 2462 2475 3569 3941 4108
Size: 393 500 263 67 13 1094 372 167
Cuts at: 4108 4217 4874 5438 5444 5629 6233 6395 6545
Size: 109 657 564 6 185 604 162 150
Cuts at: 6545 6614 6725 6962 7016 7124 7211 7300 7301
Size: 69 111 237 54 108 87 89 1
Cuts at: 7301 7747 8114 8903 9500 10287 10555 1239
Size: 446 367 789 597 787 268 1524
Fragments arranged by size:
1524 1094 789 787 657 604 597 564
500 446 393 372 367 268 263 237
185 167 162 150 111 109 108 89
87 69 67 54 13 6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2444 3641 4985 7124 7177 8093 2444
Size: 1197 1344 2139 53 916 5191
Fragments arranged by size:
5191 2139 1344 1197 916 53
EcoO109I rG'GnC_Cy
Cuts at: 2478 2740 3273 3379 3394 3599 4259 4462 4664
Size: 262 533 106 15 205 660 203 202
Cuts at: 4664 4876 5581 5588 5668 5866 6030 6687 6688
Size: 212 705 7 80 198 164 657 1
Cuts at: 6688 6906 6973 7019 7491 7918 7987 2478
Size: 218 67 46 472 427 69 5331
Fragments arranged by size:
5331 705 660 657 533 472 427 262
218 212 205 203 202 198 164 106
80 69 67 46 15 7 1
EcoRI G'AATT_C
Cuts at: 5539 6200 8149 8527 5539
Size: 661 1949 378 7852
Fragments arranged by size:
7852 1949 661 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 7087 7087
Size: 10840
FseI GG_CCGG'CC
Cuts at: 2013 2013
Size: 10840
FspI TGC'GCA
Cuts at: 7697 9346 7697
Size: 1649 9191
HaeII r_GCGC'y
Cuts at: 1678 2547 2627 2701 2841 2874 2973 3022 3068
Size: 869 80 74 140 33 99 49 46
Cuts at: 3068 3661 4566 5013 5701 6096 6120 6212 6278
Size: 593 905 447 688 395 24 92 66
Cuts at: 6278 6397 6524 6546 6592 6809 6995 7308 7950
Size: 119 127 22 46 217 186 313 642
Cuts at: 7950 9987 1678
Size: 2037 2531
Fragments arranged by size:
2531 2037 905 869 688 642 593 447
395 313 217 186 140 127 119 99
92 80 74 66 49 46 46 33
24 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2764 4079 4111 4497
Size: 32 135 32 1160 32 1315 32 386
Cuts at: 4497 4529 5041 5073 5462 5494 6081 6113 7061
Size: 32 512 32 389 32 587 32 948
Cuts at: 7061 7093 7421 7453 9481 9513 9555 9587 1373
Size: 32 328 32 2028 32 42 32 2626
Fragments arranged by size:
2626 2028 1315 1160 948 587 512 389
386 328 135 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6580 7846 8184 8430 669
Size: 988 281 4642 1266 338 246 3079
Fragments arranged by size:
4642 3079 1266 988 338 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 10840
HpaI GTT'AAC
Cuts at: 8430 8430
Size: 10840
KasI G'GCGC_C
Cuts at: 2543 2623 2837 2870 2969 3018 3064 4562 5009
Size: 80 214 33 99 49 46 1498 447
Cuts at: 5009 5697 6092 6116 6393 6542 6805 7946 2543
Size: 688 395 24 277 149 263 1141 5437
Fragments arranged by size:
5437 1498 1141 688 447 395 277 263
214 149 99 80 49 46 33 24
KpnI G_GTAC'C
Cuts at: 8165 8165
Size: 10840
MfeI C'AATT_G
Cuts at: 8439 8439
Size: 10840
MluI A'CGCG_T
Cuts at: 10716 10716
Size: 10840
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2525 2619 2775 3735 5406 6727 9954 10138
Size: 1220 94 156 960 1671 1321 3227 184
Cuts at: 10138 1305
Size: 2007
Fragments arranged by size:
3227 2007 1671 1321 1220 960 184 156
94
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2380 5285 7769 10
Size: 54 1425 343 548 2905 2484 3081
Fragments arranged by size:
3081 2905 2484 1425 548 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2374 2492 5399 8798 9157 9316 518
Size: 648 1208 118 2907 3399 359 159 2042
Fragments arranged by size:
3399 2907 2042 1208 648 359 159 118
MspA1I CmG'CkG
Cuts at: 1606 2032 2041 2289 2677 2721 2994 3243 3612
Size: 426 9 248 388 44 273 249 369
Cuts at: 3612 3791 4128 4276 4479 4548 4670 4852 5725
Size: 179 337 148 203 69 122 182 873
Cuts at: 5725 6072 7026 7136 7372 7396 7586 7857 7942
Size: 347 954 110 236 24 190 271 85
Cuts at: 7942 7957 8083 8256 8933 10081 10326 1606
Size: 15 126 173 677 1148 245 2120
Fragments arranged by size:
2120 1148 954 873 677 426 388 369
347 337 273 271 249 248 245 236
203 190 182 179 173 148 126 122
110 85 69 44 24 15 9
NaeI GCC'GGC
Cuts at: 2011 2086 3347 4067 4998 6129 6324 6547 8075
Size: 75 1261 720 931 1131 195 223 1528
Cuts at: 8075 2011
Size: 4776
Fragments arranged by size:
4776 1528 1261 1131 931 720 223 195
75
NarI GG'CG_CC
Cuts at: 2544 2624 2838 2871 2970 3019 3065 4563 5010
Size: 80 214 33 99 49 46 1498 447
Cuts at: 5010 5698 6093 6117 6394 6543 6806 7947 2544
Size: 688 395 24 277 149 263 1141 5437
Fragments arranged by size:
5437 1498 1141 688 447 395 277 263
214 149 99 80 49 46 33 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 4396 6679 6796 513
Size: 552 939 2392 2283 117 4557
Fragments arranged by size:
4557 2392 2283 939 552 117
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10840
NgoMIV G'CCGG_C
Cuts at: 2009 2084 3345 4065 4996 6127 6322 6545 8073
Size: 75 1261 720 931 1131 195 223 1528
Cuts at: 8073 2009
Size: 4776
Fragments arranged by size:
4776 1528 1261 1131 931 720 223 195
75
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10840
NotI GC'GGCC_GC
Cuts at: 2036 5720 8287 2036
Size: 3684 2567 4589
Fragments arranged by size:
4589 3684 2567
NruI TCG'CGA
Cuts at: 5071 5071
Size: 10840
NspI r_CATG'y
Cuts at: 1132 1229 2097 3742 8198 9743 1132
Size: 97 868 1645 4456 1545 2229
Fragments arranged by size:
4456 2229 1645 1545 868 97
PasI CC'CwG_GG
Cuts at: 1348 4243 4660 4970 5291 6853 7165 1348
Size: 2895 417 310 321 1562 312 5023
Fragments arranged by size:
5023 2895 1562 417 321 312 310
PciI A'CATG_T
Cuts at: 9739 9739
Size: 10840
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3074 4900 5109 6592 6703 7819 7857 1108
Size: 1966 1826 209 1483 111 1116 38 4091
Fragments arranged by size:
4091 1966 1826 1483 1116 209 111 38
PfoI T'CCnGG_A
Cuts at: 5415 5415
Size: 10840
PmeI GTTT'AAAC
Cuts at: 8144 8144
Size: 10840
PmlI CAC'GTG
Cuts at: 3748 3781 5737 7414 3748
Size: 33 1956 1677 7174
Fragments arranged by size:
7174 1956 1677 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 6989 7021 7879 7911 8909 8941 10422
Size: 32 4519 32 858 32 998 32 1481
Cuts at: 10422 10454 2438
Size: 32 2824
Fragments arranged by size:
4519 2824 1481 998 858 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2478 2740 3599 5581 6030 6973 2478
Size: 262 859 1982 449 943 6345
Fragments arranged by size:
6345 1982 943 859 449 262
PshAI GACnn'nnGTC
Cuts at: 1613 2961 7181 7625 1613
Size: 1348 4220 444 4828
Fragments arranged by size:
4828 4220 1348 444
PsiI TTA'TAA
Cuts at: 8410 8410
Size: 10840
PspOMI G'GGCC_C
Cuts at: 2026 3379 4663 4903 5588 5667 5865 6687 6905
Size: 1353 1284 240 685 79 198 822 218
Cuts at: 6905 8008 2026
Size: 1103 4858
Fragments arranged by size:
4858 1353 1284 1103 822 685 240 218
198 79
PspXI vC'TCGA_Gb
Cuts at: 3487 5258 3487
Size: 1771 9069
PstI C_TGCA'G
Cuts at: 830 2676 2765 3150 3480 4547 5730 6071 7671
Size: 1846 89 385 330 1067 1183 341 1600
Cuts at: 7671 7905 8192 830
Size: 234 287 3478
Fragments arranged by size:
3478 1846 1600 1183 1067 385 341 330
287 234 89
PvuI CG_AT'CG
Cuts at: 2001 3122 2001
Size: 1121 9719
PvuII CAG'CTG
Cuts at: 2994 3791 4128 4548 4852 6072 7372 7396 7957
Size: 797 337 420 304 1220 1300 24 561
Cuts at: 7957 2994
Size: 5877
Fragments arranged by size:
5877 1300 1220 797 561 420 337 304
24
SacII CC_GC'GG
Cuts at: 2722 4671 2722
Size: 1949 8891
SalI G'TCGA_C
Cuts at: 1936 8182 1936
Size: 6246 4594
SanDI GG'GwC_CC
Cuts at: 2478 2740 2478
Size: 262 10578
SapI GCTCTTCn'nnn_
Cuts at: 2594 6764 2594
Size: 4170 6670
SbfI CC_TGCA'GG
Cuts at: 3480 7905 8192 3480
Size: 4425 287 6128
Fragments arranged by size:
6128 4425 287
ScaI AGT'ACT
Cuts at: 9088 10800 9088
Size: 1712 9128
SexAI A'CCwGG_T
Cuts at: 2238 2319 2238
Size: 81 10759
SfcI C'TryA_G
Cuts at: 826 1045 2653 2672 2761 3146 3277 3476 4177
Size: 219 1608 19 89 385 131 199 701
Cuts at: 4177 4543 5726 6067 7667 7901 8188 9323 10004
Size: 366 1183 341 1600 234 287 1135 681
Cuts at: 10004 10195 826
Size: 191 1471
Fragments arranged by size:
1608 1600 1471 1183 1135 701 681 385
366 341 287 234 219 199 191 131
89 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 7026 7026
Size: 10840
SfoI GGC'GCC
Cuts at: 2545 2625 2839 2872 2971 3020 3066 4564 5011
Size: 80 214 33 99 49 46 1498 447
Cuts at: 5011 5699 6094 6118 6395 6544 6807 7948 2545
Size: 688 395 24 277 149 263 1141 5437
Fragments arranged by size:
5437 1498 1141 688 447 395 277 263
214 149 99 80 49 46 33 24
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10840
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10840
SmaI CCC'GGG
Cuts at: 1872 2025 2249 2476 3134 3983 4349 4615 5666
Size: 153 224 227 658 849 366 266 1051
Cuts at: 5666 6251 7463 7537 8167 1872
Size: 585 1212 74 630 4545
Fragments arranged by size:
4545 1212 1051 849 658 630 585 366
266 227 224 153 74
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3487 5258 8944 9845 10107 820
Size: 197 926 1544 1771 3686 901 262 1553
Fragments arranged by size:
3686 1771 1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4234 7297 8631 493
Size: 3741 3063 1334 2702
Fragments arranged by size:
3741 3063 2702 1334
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10840
SphI G_CATG'C
Cuts at: 1132 3742 8198 1132
Size: 2610 4456 3774
Fragments arranged by size:
4456 3774 2610
SrfI GCCC'GGGC
Cuts at: 3983 5666 3983
Size: 1683 9157
SspI AAT'ATT
Cuts at: 5 52 8764 5
Size: 47 8712 2081
Fragments arranged by size:
8712 2081 47
StuI AGG'CCT
Cuts at: 1517 6954 8027 1517
Size: 5437 1073 4330
Fragments arranged by size:
5437 4330 1073
StyI C'CwwG_G
Cuts at: 513 1065 2004 4396 4558 4806 5265 5397 5706
Size: 552 939 2392 162 248 459 132 309
Cuts at: 5706 6679 6796 7602 8022 8279 513
Size: 973 117 806 420 257 3074
Fragments arranged by size:
3074 2392 973 939 806 552 459 420
309 257 248 162 132 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5167 7664 8867 8884 9037 9222 9667
Size: 1255 3587 2497 1203 17 153 185 445
Cuts at: 9667 325
Size: 1498
Fragments arranged by size:
3587 2497 1498 1255 1203 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4535 6234 9086
Size: 274 80 33 51 1443 2558 1699 2852
Cuts at: 9086 10798 96
Size: 1712 138
Fragments arranged by size:
2852 2558 1712 1699 1443 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 3804 3836 4094 4126 6186 6218 10800
Size: 32 1592 32 258 32 2060 32 4582
Cuts at: 10800 10832 2180
Size: 32 2188
Fragments arranged by size:
4582 2188 2060 1592 258 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3086 3107 3257 3521 3911 8102 1316
Size: 1770 21 150 264 390 4191 4054
Fragments arranged by size:
4191 4054 1770 390 264 150 21
XbaI T'CTAG_A
Cuts at: 8176 8176
Size: 10840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2327 3701 5234 7186 7714 2327
Size: 1374 1533 1952 528 5453
Fragments arranged by size:
5453 1952 1533 1374 528
XhoI C'TCGA_G
Cuts at: 1943 3487 5258 1943
Size: 1544 1771 7525
Fragments arranged by size:
7525 1771 1544
XmaI C'CCGG_G
Cuts at: 1870 2023 2247 2474 3132 3981 4347 4613 5664
Size: 153 224 227 658 849 366 266 1051
Cuts at: 5664 6249 7461 7535 8165 1870
Size: 585 1212 74 630 4545
Fragments arranged by size:
4545 1212 1051 849 658 630 585 366
266 227 224 153 74
XmnI GAAnn'nnTTC
Cuts at: 2930 5558 8969 2930
Size: 2628 3411 4801
Fragments arranged by size:
4801 3411 2628
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5548 5966 6577 7432
Size: 53 83 186 936 4014 418 611 855
Cuts at: 7432 8648 276
Size: 1216 2468
Fragments arranged by size:
4014 2468 1216 936 855 611 418 186
83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
EcoICRI EcoRV FspAI NsiI PacI PsrI RsrII SacI
SwaI