Restriction map for
pFN21AB9483
(Circular) (Six-base) MAPSORT of: pFN21AB9483.seq Check: 7967 from: 1 to: 7276
pFN21AB9483 7276 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5086 278
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4559 4619 1937
Size: 2622 60 4594
Fragments arranged by size:
4594 2622 60
Acc65I G'GTAC_C
Cuts at: 4597 4597
Size: 7276
AclI AA'CG_TT
Cuts at: 1563 2866 5403 5776 1563
Size: 1303 2537 373 3063
Fragments arranged by size:
3063 2537 1303 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2157 2178 3072 3276 3309 3398 3536
Size: 464 61 21 894 204 33 89 138
Cuts at: 3536 5339 6723 6991 1632
Size: 1803 1384 268 1917
Fragments arranged by size:
1917 1803 1384 894 464 268 204 138
89 61 33 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7079
AflIII A'CryG_T
Cuts at: 1203 6175 7152 1203
Size: 4972 977 1327
Fragments arranged by size:
4972 1327 977
AgeI A'CCGG_T
Cuts at: 6107 6107
Size: 7276
AhdI GACnn_n'nnGTC
Cuts at: 3724 6005 3724
Size: 2281 4995
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2370 1184
Size: 1186 6090
AlwNI CAG_nnn'CTG
Cuts at: 3424 3514 5019 6591 3424
Size: 90 1505 1572 4109
Fragments arranged by size:
4109 1572 1505 90
ApaI G_GGCC'C
Cuts at: 4344 4344
Size: 7276
ApaLI G'TGCA_C
Cuts at: 5333 6489 5333
Size: 1156 6120
ApoI r'AATT_y
Cuts at: 1493 3958 4585 4811 4963 7072 1493
Size: 2465 627 226 152 2109 1697
Fragments arranged by size:
2465 2109 1697 627 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5830 160
Size: 872 4798 1606
Fragments arranged by size:
4798 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
AvaI C'yCGr_G
Cuts at: 1870 1943 2487 4601 1870
Size: 73 544 2114 4545
Fragments arranged by size:
4545 2114 544 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4315 4348 4315
Size: 33 7243
BamHI G'GATC_C
Cuts at: 4606 4973 4606
Size: 367 6909
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3098 4597 6052 618
Size: 325 217 627 1311 1499 1455 1842
Fragments arranged by size:
1842 1499 1455 1311 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2449 3631 4344 4437 4595 1391
Size: 1058 1182 713 93 158 4072
Fragments arranged by size:
4072 1182 1058 713 158 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2102 928
Size: 971 203 6102
Fragments arranged by size:
6102 971 203
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4178 4212 5467 5501 1903
Size: 34 2241 34 1255 34 3678
Fragments arranged by size:
3678 2241 1255 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3571 5168 6378 3571
Size: 1597 1210 4469
Fragments arranged by size:
4469 1597 1210
BclI T'GATC_A
Cuts at: 1548 2800 1548
Size: 1252 6024
BfrBI ATG'CAT
Cuts at: 4145 4145
Size: 7276
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5887 136
Size: 107 122 71 631 605 4215 1525
Fragments arranged by size:
4215 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7271 7271
Size: 7276
BlpI GC'TnA_GC
Cuts at: 4693 4693
Size: 7276
Bme1580I G_kGCm'C
Cuts at: 1790 4344 5337 6493 1790
Size: 2554 993 1156 2573
Fragments arranged by size:
2573 2554 1156 993
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7276
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2640 5007 5965
Size: 416 53 83 399 24 1223 2367 958
Cuts at: 5965 442
Size: 1753
Fragments arranged by size:
2367 1753 1223 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7276
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2578 4305 2578
Size: 1727 5549
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3681 4451 5936 1239
Size: 2442 770 1485 2579
Fragments arranged by size:
2579 2442 1485 770
Bpu10I CC'TnA_GC
Cuts at: 2343 2343
Size: 7276
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5401 6266 6564 5401
Size: 865 298 6113
Fragments arranged by size:
6113 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2462 3261 5939 882
Size: 617 963 799 2678 2219
Fragments arranged by size:
2678 2219 963 799 617
BsaAI yAC'GTr
Cuts at: 493 1204 4337 5067 493
Size: 711 3133 730 2702
Fragments arranged by size:
3133 2702 730 711
BsaBI GATnn'nnATC
Cuts at: 7270 7270
Size: 7276
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5083 5465 6100 275
Size: 53 83 186 936 3550 382 635 1451
Fragments arranged by size:
3550 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2020 4535 5709 6107 6381 6528 6939 2020
Size: 2515 1174 398 274 147 411 2357
Fragments arranged by size:
2515 2357 1174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7051
Fragments arranged by size:
7051 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2309 2327 2330 2333 2336 2339 2342
Size: 889 234 18 3 3 3 3 3
Cuts at: 2342 2505 2532 1186
Size: 163 27 5930
Fragments arranged by size:
5930 889 234 163 27 18 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 3424 4017 6479 6975 3424
Size: 593 2462 496 3725
Fragments arranged by size:
3725 2462 593 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3121 3486 1163
Size: 573 1385 365 4953
Fragments arranged by size:
4953 1385 573 365
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4726 5487 6515 1540
Size: 275 186 2725 761 1028 2301
Fragments arranged by size:
2725 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2449 3765 4437 5337 5422 6493 2449
Size: 1316 672 900 85 1071 3232
Fragments arranged by size:
3232 1316 1071 900 672 85
BsmI GAATG_Cn'
Cuts at: 4787 4880 4787
Size: 93 7183
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2449 3631 3765 4344 4437 4595 5337
Size: 399 659 1182 134 579 93 158 742
Cuts at: 5337 5422 6493 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1182 1071 742 659 579 399 158
134 93 85
BspEI T'CCGG_A
Cuts at: 2020 6939 2020
Size: 4919 2357
BspHI T'CATG_A
Cuts at: 5165 6895 5165
Size: 1730 5546
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 4631 844
Size: 2299 1488 3489
Fragments arranged by size:
3489 2299 1488
BsrBI CCG'CTC
Cuts at: 1394 1823 5163 1394
Size: 429 3340 3507
Fragments arranged by size:
3507 3340 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2609 3624 3721 5771 5945 65
Size: 1183 1361 1015 97 2050 174 1396
Fragments arranged by size:
2050 1396 1361 1183 1015 174 97
BsrFI r'CCGG_y
Cuts at: 1700 1810 5920 6107 1700
Size: 110 4110 187 2869
Fragments arranged by size:
4110 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3193 4297 96
Size: 3097 1104 3075
Fragments arranged by size:
3097 3075 1104
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7276
BssSI C'ACGA_G
Cuts at: 5336 6348 5336
Size: 1012 6264
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3491 3500 3755 794
Size: 2697 9 255 4315
Fragments arranged by size:
4315 2697 255 9
BstBI TT'CG_AA
Cuts at: 2067 6080 2067
Size: 4013 3263
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7276
BstYI r'GATC_y
Cuts at: 1972 4606 4973 5358 5375 6827 6913 6925 7271
Size: 2634 367 385 17 1452 86 12 346
Cuts at: 7271 1972
Size: 1977
Fragments arranged by size:
2634 1977 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 4560 4560
Size: 7276
Bsu36I CC'TnA_GG
Cuts at: 3322 3322
Size: 7276
BtgI C'CryG_G
Cuts at: 513 1065 2814 2979 4372 513
Size: 552 1749 165 1393 3417
Fragments arranged by size:
3417 1749 1393 552 165
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2376 4058 487
Size: 683 56 1150 1682 3705
Fragments arranged by size:
3705 1682 1150 683 56
BtsI GCAGTG_nn'
Cuts at: 2352 4531 4792 5586 5606 2352
Size: 2179 261 794 20 4022
Fragments arranged by size:
4022 2179 794 261 20
ClaI AT'CG_AT
Cuts at: 1554 4321 1554
Size: 2767 4509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3366 3401 529
Size: 35 2802 35 4404
Fragments arranged by size:
4404 2802 35 35
DraI TTT'AAA
Cuts at: 2640 2838 3315 3609 4580 2640
Size: 198 477 294 971 5336
Fragments arranged by size:
5336 971 477 294 198
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2207 6283 809
Size: 507 891 4076 1802
Fragments arranged by size:
4076 1802 891 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2811 4723 5612
Size: 54 1425 50 275 18 981 1912 889
Cuts at: 5612 7241 8
Size: 1629 43
Fragments arranged by size:
1912 1629 1425 981 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4723 1537
Size: 275 2911 4090
Fragments arranged by size:
4090 2911 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2295 2304 2496 3102 4480 5206 1353
Size: 723 219 9 192 606 1378 726 3423
Fragments arranged by size:
3423 1378 726 723 606 219 192 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2089 5863 6237 6383 387
Size: 1064 138 500 3774 374 146 1280
Fragments arranged by size:
3774 1280 1064 500 374 146 138
EcoICRI GAG'CTC
Cuts at: 2447 4435 2447
Size: 1988 5288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2157 2178 3072 3276 3309 3398
Size: 393 464 61 21 894 204 33 89
Cuts at: 3398 3536 3681 4451 5339 5936 6723 6991 1239
Size: 138 145 770 888 597 787 268 1524
Fragments arranged by size:
1524 894 888 787 770 597 464 393
268 204 145 138 89 61 33 21
EcoO109I rG'GnC_Cy
Cuts at: 2968 4341 4426 2968
Size: 1373 85 5818
Fragments arranged by size:
5818 1373 85
EcoRI G'AATT_C
Cuts at: 4585 4963 4585
Size: 378 6898
EcoRV GAT'ATC
Cuts at: 3166 3645 3166
Size: 479 6797
FspI TGC'GCA
Cuts at: 2634 5782 2634
Size: 3148 4128
HaeII r_GCGC'y
Cuts at: 1678 6423 1678
Size: 4745 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2785 2817 3689 3710 3721
Size: 32 135 32 1213 32 872 21 11
Cuts at: 3721 3742 5917 5949 5991 6023 1373
Size: 21 2175 32 42 32 2626
Fragments arranged by size:
2626 2175 1213 872 135 42 32 32
32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2992 4620 4866 669
Size: 988 281 1054 1628 246 3079
Fragments arranged by size:
3079 1628 1054 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2949 3036 3291 748
Size: 2201 87 255 4733
Fragments arranged by size:
4733 2201 255 87
HpaI GTT'AAC
Cuts at: 2992 4866 2992
Size: 1874 5402
KpnI G_GTAC'C
Cuts at: 4601 4601
Size: 7276
MfeI C'AATT_G
Cuts at: 4875 4875
Size: 7276
MluI A'CGCG_T
Cuts at: 7152 7152
Size: 7276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6390 6574 1305
Size: 5085 184 2007
Fragments arranged by size:
5085 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2813 10
Size: 54 1425 343 981 4473
Fragments arranged by size:
4473 1425 981 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3387 5234 5593 5752 518
Size: 648 2221 1847 359 159 2042
Fragments arranged by size:
2221 2042 1847 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 2878 3002 3424 4692 5369 6517 6762
Size: 529 743 124 422 1268 677 1148 245
Cuts at: 6762 1606
Size: 2120
Fragments arranged by size:
2120 1268 1148 743 677 529 422 245
124
NcoI C'CATG_G
Cuts at: 513 1065 2814 2979 513
Size: 552 1749 165 4810
Fragments arranged by size:
4810 1749 552 165
NdeI CA'TA_TG
Cuts at: 387 4254 387
Size: 3867 3409
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7276
NotI GC'GGCC_GC
Cuts at: 4723 4723
Size: 7276
NsiI A_TGCA'T
Cuts at: 4147 4147
Size: 7276
NspI r_CATG'y
Cuts at: 1132 1229 4172 4289 4634 6179 1132
Size: 97 2943 117 345 1545 2229
Fragments arranged by size:
2943 2229 1545 345 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7276
PciI A'CATG_T
Cuts at: 6175 6175
Size: 7276
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2858 1108
Size: 1750 5526
PmeI GTTT'AAAC
Cuts at: 4580 4580
Size: 7276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5345 5377 6858 6890 5345
Size: 32 1481 32 5731
Fragments arranged by size:
5731 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7276
PsiI TTA'TAA
Cuts at: 4846 4846
Size: 7276
PspOMI G'GGCC_C
Cuts at: 4340 4340
Size: 7276
PspXI vC'TCGA_Gb
Cuts at: 2487 2487
Size: 7276
PstI C_TGCA'G
Cuts at: 830 4628 830
Size: 3798 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7276
PvuII CAG'CTG
Cuts at: 2135 2878 3002 3424 2135
Size: 743 124 422 5987
Fragments arranged by size:
5987 743 422 124
SacI G_AGCT'C
Cuts at: 2449 4437 2449
Size: 1988 5288
SalI G'TCGA_C
Cuts at: 1936 4618 1936
Size: 2682 4594
SbfI CC_TGCA'GG
Cuts at: 4628 4628
Size: 7276
ScaI AGT'ACT
Cuts at: 5524 7236 5524
Size: 1712 5564
SfcI C'TryA_G
Cuts at: 826 1045 4222 4572 4624 5759 6440 6631 826
Size: 219 3177 350 52 1135 681 191 1471
Fragments arranged by size:
3177 1471 1135 681 350 219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7276
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7276
SmaI CCC'GGG
Cuts at: 1872 4603 1872
Size: 2731 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2487 5380 6281 6543 820
Size: 197 926 544 2893 901 262 1553
Fragments arranged by size:
2893 1553 926 901 544 262 197
SnaBI TAC'GTA
Cuts at: 493 5067 493
Size: 4574 2702
SpeI A'CTAG_T
Cuts at: 152 3238 152
Size: 3086 4190
SphI G_CATG'C
Cuts at: 1132 4172 4634 1132
Size: 3040 462 3774
Fragments arranged by size:
3774 3040 462
SspI AAT'ATT
Cuts at: 5 52 2701 3753 3881 5200 5
Size: 47 2649 1052 128 1319 2081
Fragments arranged by size:
2649 2081 1319 1052 128 47
StuI AGG'CCT
Cuts at: 1517 2425 3320 4239 1517
Size: 908 895 919 4554
Fragments arranged by size:
4554 919 908 895
StyI C'CwwG_G
Cuts at: 513 1065 2590 2814 2979 4715 513
Size: 552 1525 224 165 1736 3074
Fragments arranged by size:
3074 1736 1525 552 224 165
SwaI ATTT'AAAT
Cuts at: 2640 2640
Size: 7276
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 2786 5303 5320 5473 5658 6103
Size: 1255 873 333 2517 17 153 185 445
Cuts at: 6103 325
Size: 1498
Fragments arranged by size:
2517 1498 1255 873 445 333 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3193 4297 5522
Size: 274 80 33 51 1443 1216 1104 1225
Cuts at: 5522 7234 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1225 1216 1104 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3776 3808 4170 4202 7236 7268 3776
Size: 32 362 32 3034 32 3784
Fragments arranged by size:
3784 3034 362 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7276
XbaI T'CTAG_A
Cuts at: 4612 4612
Size: 7276
XhoI C'TCGA_G
Cuts at: 1943 2487 1943
Size: 544 6732
XmaI C'CCGG_G
Cuts at: 1870 4601 1870
Size: 2731 4545
XmnI GAAnn'nnTTC
Cuts at: 3294 4031 5405 3294
Size: 737 1374 5165
Fragments arranged by size:
5165 1374 737
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5084 276
Size: 53 83 186 936 3550 2468
Fragments arranged by size:
3550 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BsiWI
BsmBI BstXI DraIII EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI XcmI