Restriction map for
pFN21AB9482
(Circular) (Six-base) MAPSORT of: pFN21AB9482.seq Check: 5690 from: 1 to: 6256
pFN21AB9482 6256 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3352 3352
Size: 6256
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4066 278
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 3599 1937
Size: 1374 288 4594
Fragments arranged by size:
4594 1374 288
Acc65I G'GTAC_C
Cuts at: 3577 3577
Size: 6256
AclI AA'CG_TT
Cuts at: 1563 4383 4756 1563
Size: 2820 373 3063
Fragments arranged by size:
3063 2820 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2509 4319 5703 5971 1632
Size: 877 1810 1384 268 1917
Fragments arranged by size:
1917 1810 1384 877 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6059
AflIII A'CryG_T
Cuts at: 1203 2367 2823 3477 5155 6132 1203
Size: 1164 456 654 1678 977 1327
Fragments arranged by size:
1678 1327 1164 977 654 456
AgeI A'CCGG_T
Cuts at: 5087 5087
Size: 6256
AhdI GACnn_n'nnGTC
Cuts at: 2824 4985 2824
Size: 2161 4095
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6256
AlwNI CAG_nnn'CTG
Cuts at: 3298 3999 5571 3298
Size: 701 1572 3983
Fragments arranged by size:
3983 1572 701
ApaI G_GGCC'C
Cuts at: 2898 2898
Size: 6256
ApaLI G'TGCA_C
Cuts at: 2423 4313 5469 2423
Size: 1890 1156 3210
Fragments arranged by size:
3210 1890 1156
ApoI r'AATT_y
Cuts at: 1493 3565 3791 3943 6052 1493
Size: 2072 226 152 2109 1697
Fragments arranged by size:
2109 2072 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4810 160
Size: 872 3778 1606
Fragments arranged by size:
3778 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
AvaI C'yCGr_G
Cuts at: 1870 1943 2282 3227 3509 3581 1870
Size: 73 339 945 282 72 4545
Fragments arranged by size:
4545 945 339 282 73 72
AvrII C'CTAG_G
Cuts at: 2014 2014
Size: 6256
BamHI G'GATC_C
Cuts at: 3586 3953 3586
Size: 367 5889
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2716 2875 2989 3577 5032
Size: 325 217 627 929 159 114 588 1455
Cuts at: 5032 618
Size: 1842
Fragments arranged by size:
1842 1455 929 627 588 325 217 159
114
BanII G_rGCy'C
Cuts at: 1391 2898 3146 3575 1391
Size: 1507 248 429 4072
Fragments arranged by size:
4072 1507 429 248
BbeI G_GCGC'C
Cuts at: 2720 2879 2720
Size: 159 6097
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3110 3452 928
Size: 971 1211 342 3732
Fragments arranged by size:
3732 1211 971 342
BbvCI CC'TCA_GC
Cuts at: 3079 3079
Size: 6256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2599 2633 3239 3273 4447 4481 1903
Size: 34 662 34 606 34 1174 34 3678
Fragments arranged by size:
3678 1174 662 606 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4148 5358 4148
Size: 1210 5046
BclI T'GATC_A
Cuts at: 1548 3522 1548
Size: 1974 4282
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2411 2480
Size: 107 122 71 631 605 337 402 69
Cuts at: 2480 3165 4867 136
Size: 685 1702 1525
Fragments arranged by size:
1702 1525 685 631 605 402 337 122
107 71 69
BglII A'GATC_T
Cuts at: 6251 6251
Size: 6256
BlpI GC'TnA_GC
Cuts at: 3455 3673 3455
Size: 218 6038
Bme1580I G_kGCm'C
Cuts at: 1790 2396 2427 2898 4317 5473 1790
Size: 606 31 471 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 606 471 31
BmgBI CAC'GTC
Cuts at: 1318 2370 3358 1318
Size: 1052 988 4216
Fragments arranged by size:
4216 1052 988
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2383 3987 4945
Size: 416 53 83 399 24 966 1604 958
Cuts at: 4945 442
Size: 1753
Fragments arranged by size:
1753 1604 966 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6256
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 6256
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2165 2654 2666 2821 4916 1239
Size: 926 489 12 155 2095 2579
Fragments arranged by size:
2579 2095 926 489 155 12
Bpu10I CC'TnA_GC
Cuts at: 3079 3079
Size: 6256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2176 2252 2534 3130 3479 3533 4381 5246 5544
Size: 76 282 596 349 54 848 865 298
Cuts at: 5544 2176
Size: 2888
Fragments arranged by size:
2888 865 848 596 349 298 282 76
54
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4919 882
Size: 617 3420 2219
Fragments arranged by size:
3420 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2127 4047 493
Size: 711 923 1920 2702
Fragments arranged by size:
2702 1920 923 711
BsaBI GATnn'nnATC
Cuts at: 6250 6250
Size: 6256
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2717 2876 3107 4063
Size: 53 83 186 936 1184 159 231 956
Cuts at: 4063 4445 5080 275
Size: 382 635 1451
Fragments arranged by size:
1451 1184 956 936 635 382 231 186
159 83 53
BsaWI w'CCGG_w
Cuts at: 4689 5087 5361 5508 5919 4689
Size: 398 274 147 411 5026
Fragments arranged by size:
5026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2639 2669 1178
Size: 30 165 30 1236 30 4765
Fragments arranged by size:
4765 1236 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2198 2249 2492 2501 2504 2515 2591
Size: 1009 3 51 243 9 3 11 76
Cuts at: 2591 2594 2696 2903 2945 2954 2957 2972 2984
Size: 3 102 207 42 9 3 15 12
Cuts at: 2984 2987 2990 2993 3047 3065 3067 3187 1186
Size: 3 3 3 54 18 2 120 4255
Fragments arranged by size:
4255 1009 243 207 120 102 76 54
51 42 18 15 12 11 9 9
3 3 3 3 3 3 3 2
BseYI C'CCAG_C
Cuts at: 3173 3318 5459 5955 3173
Size: 145 2141 496 3474
Fragments arranged by size:
3474 2141 496 145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3380 1163
Size: 573 1644 4039
Fragments arranged by size:
4039 1644 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3706 4467 5495 1540
Size: 275 186 1705 761 1028 2301
Fragments arranged by size:
2301 1705 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2427 3146 4317 4402 5473 2427
Size: 719 1171 85 1071 3210
Fragments arranged by size:
3210 1171 1071 719 85
BsmI GAATG_Cn'
Cuts at: 3767 3860 3767
Size: 93 6163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2396 2427 2898 3146 3575 4317 4402
Size: 399 606 31 471 248 429 742 85
Cuts at: 4402 5473 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 606 471 429 399 248
85 31
BspEI T'CCGG_A
Cuts at: 5919 5919
Size: 6256
BspHI T'CATG_A
Cuts at: 4145 5875 4145
Size: 1730 4526
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3352 3429 3611 844
Size: 2508 77 182 3489
Fragments arranged by size:
3489 2508 182 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2149 3124 4143 1394
Size: 429 326 975 1019 3507
Fragments arranged by size:
3507 1019 975 429 326
BsrDI GCAATG_nn'
Cuts at: 65 1248 4751 4925 65
Size: 1183 3503 174 1396
Fragments arranged by size:
3503 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4900 5087 1700
Size: 110 3090 187 2869
Fragments arranged by size:
3090 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6256
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6256
BssSI C'ACGA_G
Cuts at: 4316 5328 4316
Size: 1012 5244
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6256
BstBI TT'CG_AA
Cuts at: 5060 5060
Size: 6256
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6256
BstYI r'GATC_y
Cuts at: 1972 3489 3586 3953 4338 4355 5807 5893 5905
Size: 1517 97 367 385 17 1452 86 12
Cuts at: 5905 6251 1972
Size: 346 1977
Fragments arranged by size:
1977 1517 1452 385 367 346 97 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2955 2955
Size: 6256
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2444 2802 487
Size: 683 56 1218 358 3941
Fragments arranged by size:
3941 1218 683 358 56
BtsI GCAGTG_nn'
Cuts at: 2215 2469 3772 4566 4586 2215
Size: 254 1303 794 20 3885
Fragments arranged by size:
3885 1303 794 254 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6256
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6221
DraI TTT'AAA
Cuts at: 3560 3560
Size: 6256
DraIII CAC_nnn'GTG
Cuts at: 2391 2391
Size: 6256
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5263 809
Size: 507 3947 1802
Fragments arranged by size:
3947 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2596 2724 3703
Size: 54 1425 50 275 18 766 128 979
Cuts at: 3703 4592 6221 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 979 889 766 275 128 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3703 1537
Size: 275 1891 4090
Fragments arranged by size:
4090 1891 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2847 3092 4186 1353
Size: 1494 245 1094 3423
Fragments arranged by size:
3423 1494 1094 245
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4843 5217 5363 387
Size: 1064 138 3254 374 146 1280
Fragments arranged by size:
3254 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3144 3144
Size: 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2165 2509 2654 2666 2821 4319 4916
Size: 393 533 344 145 12 155 1498 597
Cuts at: 4916 5703 5971 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1498 787 597 533 393 344 268
155 145 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3551 3551
Size: 6256
EcoO109I rG'GnC_Cy
Cuts at: 2018 2403 2894 3287 3418 2018
Size: 385 491 393 131 4856
Fragments arranged by size:
4856 491 393 385 131
EcoRI G'AATT_C
Cuts at: 3565 3943 3565
Size: 378 5878
FspI TGC'GCA
Cuts at: 2621 4762 2621
Size: 2141 4115
HaeII r_GCGC'y
Cuts at: 1678 2720 2879 5403 1678
Size: 1042 159 2524 2531
Fragments arranged by size:
2531 2524 1042 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2354 2386 2639 2671 2810
Size: 32 135 32 782 32 253 32 139
Cuts at: 2810 2842 4897 4929 4971 5003 1373
Size: 32 2055 32 42 32 2626
Fragments arranged by size:
2626 2055 782 253 139 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2116 2311 3312 3600 3846 669
Size: 988 281 178 195 1001 288 246 3079
Fragments arranged by size:
3079 1001 988 288 281 246 195 178
HindIII A'AGCT_T
Cuts at: 748 2225 748
Size: 1477 4779
HpaI GTT'AAC
Cuts at: 3846 3846
Size: 6256
KasI G'GCGC_C
Cuts at: 2716 2875 2716
Size: 159 6097
KpnI G_GTAC'C
Cuts at: 3581 3581
Size: 6256
MfeI C'AATT_G
Cuts at: 3855 3855
Size: 6256
MluI A'CGCG_T
Cuts at: 6132 6132
Size: 6256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3018 5370 5554 1305
Size: 1713 2352 184 2007
Fragments arranged by size:
2352 2007 1713 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2726 10
Size: 54 1425 343 894 3540
Fragments arranged by size:
3540 1425 894 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4214 4573 4732 518
Size: 648 3048 359 159 2042
Fragments arranged by size:
3048 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2542 2722 3351 3672 4349 5497 5742
Size: 843 93 180 629 321 677 1148 245
Cuts at: 5742 1606
Size: 2120
Fragments arranged by size:
2120 1148 843 677 629 321 245 180
93
NarI GG'CG_CC
Cuts at: 2717 2876 2717
Size: 159 6097
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4765
Fragments arranged by size:
4765 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6256
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6256
NotI GC'GGCC_GC
Cuts at: 3703 3703
Size: 6256
NspI r_CATG'y
Cuts at: 1132 1229 2827 3614 5159 1132
Size: 97 1598 787 1545 2229
Fragments arranged by size:
2229 1598 1545 787 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6256
PciI A'CATG_T
Cuts at: 2823 5155 2823
Size: 2332 3924
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 2815 1108
Size: 1524 183 4549
Fragments arranged by size:
4549 1524 183
PmeI GTTT'AAAC
Cuts at: 3560 3560
Size: 6256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4325 4357 5838 5870 4325
Size: 32 1481 32 4711
Fragments arranged by size:
4711 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3287 3418 3287
Size: 131 6125
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6256
PsiI TTA'TAA
Cuts at: 3826 3826
Size: 6256
PspOMI G'GGCC_C
Cuts at: 2894 2894
Size: 6256
PspXI vC'TCGA_Gb
Cuts at: 3227 3227
Size: 6256
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2357 2389 2357
Size: 32 6224
PstI C_TGCA'G
Cuts at: 830 3262 3608 830
Size: 2432 346 3478
Fragments arranged by size:
3478 2432 346
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6256
SacI G_AGCT'C
Cuts at: 3146 3146
Size: 6256
SalI G'TCGA_C
Cuts at: 1936 3310 3598 1936
Size: 1374 288 4594
Fragments arranged by size:
4594 1374 288
SanDI GG'GwC_CC
Cuts at: 3287 3287
Size: 6256
SapI GCTCTTCn'nnn_
Cuts at: 2847 2847
Size: 6256
SbfI CC_TGCA'GG
Cuts at: 3608 3608
Size: 6256
ScaI AGT'ACT
Cuts at: 4504 6216 4504
Size: 1712 4544
SfcI C'TryA_G
Cuts at: 826 1045 3258 3604 4739 5420 5611 826
Size: 219 2213 346 1135 681 191 1471
Fragments arranged by size:
2213 1471 1135 681 346 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2411 2411
Size: 6256
SfoI GGC'GCC
Cuts at: 2718 2877 2718
Size: 159 6097
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6256
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6256
SmaI CCC'GGG
Cuts at: 1872 3583 1872
Size: 1711 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2155 2267 2549 3145 3227 3494
Size: 197 926 212 112 282 596 82 267
Cuts at: 3494 3548 4360 5261 5523 820
Size: 54 812 901 262 1553
Fragments arranged by size:
1553 926 901 812 596 282 267 262
212 197 112 82 54
SnaBI TAC'GTA
Cuts at: 493 4047 493
Size: 3554 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6256
SphI G_CATG'C
Cuts at: 1132 3614 1132
Size: 2482 3774
SspI AAT'ATT
Cuts at: 5 52 4180 5
Size: 47 4128 2081
Fragments arranged by size:
4128 2081 47
StuI AGG'CCT
Cuts at: 1517 2414 2774 3074 1517
Size: 897 360 300 4699
Fragments arranged by size:
4699 897 360 300
StyI C'CwwG_G
Cuts at: 513 1065 2004 2014 2700 2727 2871 2898 3695
Size: 552 939 10 686 27 144 27 797
Cuts at: 3695 513
Size: 3074
Fragments arranged by size:
3074 939 797 686 552 144 27 27
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 3007 3127 3358 4283 4300 4453
Size: 1255 822 605 120 231 925 17 153
Cuts at: 4453 4638 5083 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 925 822 605 445 231 185
153 120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4502 6214 96
Size: 274 80 33 51 1443 2525 1712 138
Fragments arranged by size:
2525 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6216 6248 6216
Size: 32 6224
Tth111I GACn'n_nGTC
Cuts at: 1316 2111 1316
Size: 795 5461
XbaI T'CTAG_A
Cuts at: 3592 3592
Size: 6256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3299 3299
Size: 6256
XhoI C'TCGA_G
Cuts at: 1943 3227 1943
Size: 1284 4972
XmaI C'CCGG_G
Cuts at: 1870 3581 1870
Size: 1711 4545
XmnI GAAnn'nnTTC
Cuts at: 2522 3431 4385 2522
Size: 909 954 4393
Fragments arranged by size:
4393 954 909
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4064 276
Size: 53 83 186 936 2530 2468
Fragments arranged by size:
2530 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PvuII RsrII SacII SexAI
SrfI SwaI