Restriction map for
pFN21AB9481
(Circular) (Six-base) MAPSORT of: pFN21AB9481.seq Check: 7789 from: 1 to: 11350
pFN21AB9481 11350 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9160 278
Size: 53 83 186 936 7624 2468
Fragments arranged by size:
7624 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2678 3712 5196 8520 8693 1937
Size: 741 1034 1484 3324 173 4594
Fragments arranged by size:
4594 3324 1484 1034 741 173
Acc65I G'GTAC_C
Cuts at: 6918 8671 6918
Size: 1753 9597
AclI AA'CG_TT
Cuts at: 1563 3229 8040 9477 9850 1563
Size: 1666 4811 1437 373 3063
Fragments arranged by size:
4811 3063 1666 1437 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2032 2727 3563 4281 4738 4813 5627 5916
Size: 400 695 836 718 457 75 814 289
Cuts at: 5916 7181 7391 7454 7945 9413 10797 11065 1632
Size: 1265 210 63 491 1468 1384 268 1917
Fragments arranged by size:
1917 1468 1384 1265 836 814 718 695
491 457 400 289 268 210 75 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11153
AflIII A'CryG_T
Cuts at: 1203 5541 10249 11226 1203
Size: 4338 4708 977 1327
Fragments arranged by size:
4708 4338 1327 977
AgeI A'CCGG_T
Cuts at: 10181 10181
Size: 11350
AhdI GACnn_n'nnGTC
Cuts at: 4365 8508 10079 4365
Size: 4143 1571 5636
Fragments arranged by size:
5636 4143 1571
AleI CACnn'nnGTG
Cuts at: 1166 7023 7127 7886 8360 8370 1166
Size: 5857 104 759 474 10 4146
Fragments arranged by size:
5857 4146 759 474 104 10
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5730 8388 1184
Size: 4546 2658 4146
Fragments arranged by size:
4546 4146 2658
AlwNI CAG_nnn'CTG
Cuts at: 4319 4850 5120 5648 5918 6182 6251 8296 9093
Size: 531 270 528 270 264 69 2045 797
Cuts at: 9093 10665 4319
Size: 1572 5004
Fragments arranged by size:
5004 2045 1572 797 531 528 270 270
264 69
ApaI G_GGCC'C
Cuts at: 6510 8355 6510
Size: 1845 9505
ApaLI G'TGCA_C
Cuts at: 8363 8465 9407 10563 8363
Size: 102 942 1156 9150
Fragments arranged by size:
9150 1156 942 102
ApoI r'AATT_y
Cuts at: 1493 2015 2796 3132 4018 4027 4214 4288 4406
Size: 522 781 336 886 9 187 74 118
Cuts at: 4406 5313 5810 7352 7439 8659 8885 9037 11146
Size: 907 497 1542 87 1220 226 152 2109
Cuts at: 11146 1493
Size: 1697
Fragments arranged by size:
2109 1697 1542 1220 907 886 781 522
497 336 226 187 152 118 87 74
9
AseI AT'TA_AT
Cuts at: 160 1032 9904 160
Size: 872 8872 1606
Fragments arranged by size:
8872 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11350
AvaI C'yCGr_G
Cuts at: 1870 1943 8341 8675 1870
Size: 73 6398 334 4545
Fragments arranged by size:
6398 4545 334 73
AvrII C'CTAG_G
Cuts at: 7321 7321
Size: 11350
BamHI G'GATC_C
Cuts at: 3378 3885 6783 8680 9047 3378
Size: 507 2898 1897 367 5681
Fragments arranged by size:
5681 2898 1897 507 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2272 4234 4657 5955 6918
Size: 325 217 627 485 1962 423 1298 963
Cuts at: 6918 8000 8671 10126 618
Size: 1082 671 1455 1842
Fragments arranged by size:
1962 1842 1455 1298 1082 963 671 627
485 423 325 217
BanII G_rGCy'C
Cuts at: 1391 2129 2807 3972 5994 6049 6510 7503 8355
Size: 738 678 1165 2022 55 461 993 852
Cuts at: 8355 8669 1391
Size: 314 4072
Fragments arranged by size:
4072 2022 1165 993 852 738 678 461
314 55
BbeI G_GCGC'C
Cuts at: 4661 4661
Size: 11350
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3029 3988 6565 6614 6978 7050 928
Size: 971 1130 959 2577 49 364 72 5228
Fragments arranged by size:
5228 2577 1130 971 959 364 72 49
BbvCI CC'TCA_GC
Cuts at: 4637 4637
Size: 11350
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2268 2302 2457 2491 9541 9575 1903
Size: 34 331 34 155 34 7050 34 3678
Fragments arranged by size:
7050 3678 331 155 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4918 9242 10452 4918
Size: 4324 1210 5816
Fragments arranged by size:
5816 4324 1210
BclI T'GATC_A
Cuts at: 1548 2293 4936 1548
Size: 745 2643 7962
Fragments arranged by size:
7962 2643 745
BfrBI ATG'CAT
Cuts at: 5675 5675
Size: 11350
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 9961 136
Size: 107 122 71 631 605 337 7952 1525
Fragments arranged by size:
7952 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5818 6400 11345 5818
Size: 582 4945 5823
Fragments arranged by size:
5823 4945 582
BlpI GC'TnA_GC
Cuts at: 2245 5993 8767 2245
Size: 3748 2774 4828
Fragments arranged by size:
4828 3748 2774
Bme1580I G_kGCm'C
Cuts at: 1790 2864 6510 6909 8355 8367 8469 9411 10567
Size: 1074 3646 399 1446 12 102 942 1156
Cuts at: 10567 1790
Size: 2573
Fragments arranged by size:
3646 2573 1446 1156 1074 942 399 102
12
BmgBI CAC'GTC
Cuts at: 1318 5530 7795 1318
Size: 4212 2265 4873
Fragments arranged by size:
4873 4212 2265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3880 6444 8059
Size: 416 53 83 399 24 2463 2564 1615
Cuts at: 8059 8284 9081 10039 442
Size: 225 797 958 1753
Fragments arranged by size:
2564 2463 1753 1615 958 797 416 399
225 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11350
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7910 8564 7910
Size: 654 10696
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2321 2335 2680 2752 3240 3255 3827 3885
Size: 1082 14 345 72 488 15 572 58
Cuts at: 3885 4055 4447 4947 5576 6020 6259 6887 7217
Size: 170 392 500 629 444 239 628 330
Cuts at: 7217 8405 10010 1239
Size: 1188 1605 2579
Fragments arranged by size:
2579 1605 1188 1082 629 628 572 500
488 444 392 345 330 239 170 72
58 15 14
Bpu10I CC'TnA_GC
Cuts at: 4637 4637
Size: 11350
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 4481 6033 9475 10340 10638 2330
Size: 2151 1552 3442 865 298 3042
Fragments arranged by size:
3442 3042 2151 1552 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3840 4164 4463 10013 882
Size: 617 2341 324 299 5550 2219
Fragments arranged by size:
5550 2341 2219 617 324 299
BsaAI yAC'GTr
Cuts at: 493 1204 6373 6498 9141 493
Size: 711 5169 125 2643 2702
Fragments arranged by size:
5169 2702 2643 711 125
BsaBI GATnn'nnATC
Cuts at: 5849 6030 7911 11344 5849
Size: 181 1881 3433 5855
Fragments arranged by size:
5855 3433 1881 181
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4658 9157 9539 10174
Size: 53 83 186 936 3125 4499 382 635
Cuts at: 10174 275
Size: 1451
Fragments arranged by size:
4499 3125 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3065 4619 7686 9783 10181 10455 10602 11013 3065
Size: 1554 3067 2097 398 274 147 411 3402
Fragments arranged by size:
3402 3067 2097 1554 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2457 2487 4593 4623 4857
Size: 30 165 30 1054 30 2106 30 234
Cuts at: 4857 4887 6326 6356 7199 7229 7262 7292 1178
Size: 30 1439 30 843 30 33 30 5236
Fragments arranged by size:
5236 2106 1439 1054 843 234 165 33
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2497 6996 7796 1186
Size: 1311 4499 800 4740
Fragments arranged by size:
4740 4499 1311 800
BseYI C'CCAG_C
Cuts at: 5911 6175 8348 10553 11049 5911
Size: 264 2173 2205 496 6212
Fragments arranged by size:
6212 2205 2173 496 264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2184 2624 6959 1163
Size: 573 448 440 4335 5554
Fragments arranged by size:
5554 4335 573 448 440
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3712 4624 6948 8800 9561 10589
Size: 275 186 1711 912 2324 1852 761 1028
Cuts at: 10589 1540
Size: 2301
Fragments arranged by size:
2324 2301 1852 1711 1028 912 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 2489 5153 5541 5994 6049 8367 8469 9411 9496
Size: 2664 388 453 55 2318 102 942 85
Cuts at: 9496 10567 2489
Size: 1071 3272
Fragments arranged by size:
3272 2664 2318 1071 942 453 388 102
85 55
BsmI GAATG_Cn'
Cuts at: 4989 5483 6827 7398 7632 8861 8954 4989
Size: 494 1344 571 234 1229 93 7385
Fragments arranged by size:
7385 1344 1229 571 494 234 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4392 5451 4392
Size: 1059 10291
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2129 2489 2807 2864 3972 5153 5541
Size: 399 339 360 318 57 1108 1181 388
Cuts at: 5541 5994 6049 6510 6909 7503 8355 8367 8469
Size: 453 55 461 399 594 852 12 102
Cuts at: 8469 8669 9411 9496 10567 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1181 1108 1071 852 742 594 461
453 399 399 388 360 339 318 200
102 85 57 55 12
BspEI T'CCGG_A
Cuts at: 3065 11013 3065
Size: 7948 3402
BspHI T'CATG_A
Cuts at: 4696 5580 6894 7734 9239 10969 4696
Size: 884 1314 840 1505 1730 5077
Fragments arranged by size:
5077 1730 1505 1314 884 840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2045 2294 6747 8705 844
Size: 1201 249 4453 1958 3489
Fragments arranged by size:
4453 3489 1958 1201 249
BsrBI CCG'CTC
Cuts at: 1394 1823 2244 4840 9237 1394
Size: 429 421 2596 4397 3507
Fragments arranged by size:
4397 3507 2596 429 421
BsrDI GCAATG_nn'
Cuts at: 65 1248 4725 5135 6471 6742 6768 7818 9845
Size: 1183 3477 410 1336 271 26 1050 2027
Cuts at: 9845 10019 65
Size: 174 1396
Fragments arranged by size:
3477 2027 1396 1336 1183 1050 410 271
174 26
BsrFI r'CCGG_y
Cuts at: 1700 1810 3035 3354 6747 6947 9994 10181 1700
Size: 110 1225 319 3393 200 3047 187 2869
Fragments arranged by size:
3393 3047 2869 1225 319 200 187 110
BsrGI T'GTAC_A
Cuts at: 96 5043 5341 6015 6105 96
Size: 4947 298 674 90 5341
Fragments arranged by size:
5341 4947 674 298 90
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11350
BssSI C'ACGA_G
Cuts at: 9410 10422 9410
Size: 1012 10338
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5672 6550 794
Size: 4878 878 5594
Fragments arranged by size:
5594 4878 878
BstBI TT'CG_AA
Cuts at: 10154 10154
Size: 11350
BstEII G'GTnAC_C
Cuts at: 1184 7682 8059 1184
Size: 6498 377 4475
Fragments arranged by size:
6498 4475 377
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 3335 6865 6964 2359
Size: 976 3530 99 6745
Fragments arranged by size:
6745 3530 976 99
BstYI r'GATC_y
Cuts at: 1972 2983 3378 3885 4545 5128 5208 5818 5850
Size: 1011 395 507 660 583 80 610 32
Cuts at: 5850 6400 6635 6704 6783 8629 8680 9047 9432
Size: 550 235 69 79 1846 51 367 385
Cuts at: 9432 9449 10901 10987 10999 11345 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1846 1452 1011 660 610 583 550
507 395 385 367 346 235 86 80
79 69 51 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 5930 5930
Size: 11350
BtgI C'CryG_G
Cuts at: 513 1065 2004 2230 3328 3525 4518 6762 7600
Size: 552 939 226 1098 197 993 2244 838
Cuts at: 7600 8269 513
Size: 669 3594
Fragments arranged by size:
3594 2244 1098 993 939 838 669 552
226 197
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10611
Fragments arranged by size:
10611 683 56
BtsI GCAGTG_nn'
Cuts at: 2162 2842 3177 3777 6551 7156 7380 8365 8377
Size: 680 335 600 2774 605 224 985 12
Cuts at: 8377 8469 8866 9660 9680 2162
Size: 92 397 794 20 3832
Fragments arranged by size:
3832 2774 985 794 680 605 600 397
335 224 92 20 12
ClaI AT'CG_AT
Cuts at: 1554 5278 7852 1554
Size: 3724 2574 5052
Fragments arranged by size:
5052 3724 2574
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 6751 6786 529
Size: 35 6187 35 5093
Fragments arranged by size:
6187 5093 35 35
DraI TTT'AAA
Cuts at: 3694 8654 3694
Size: 4960 6390
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6062 8273 10357 809
Size: 507 4746 2211 2084 1802
Fragments arranged by size:
4746 2211 2084 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 4344 6489
Size: 54 1425 50 275 18 381 2133 2145
Cuts at: 6489 6765 6945 8105 8261 8797 9686 11315 8
Size: 276 180 1160 156 536 889 1629 43
Fragments arranged by size:
2145 2133 1629 1425 1160 889 536 381
276 275 180 156 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6945 8797 1537
Size: 275 5133 1852 4090
Fragments arranged by size:
5133 4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2230 2703 2952 3899 4300 4555 5225 7960
Size: 877 473 249 947 401 255 670 2735
Cuts at: 7960 9280 1353
Size: 1320 3423
Fragments arranged by size:
3423 2735 1320 947 877 670 473 401
255 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2738 2768 5647 8228 9937 10311
Size: 1064 138 1149 30 2879 2581 1709 374
Cuts at: 10311 10457 387
Size: 146 1280
Fragments arranged by size:
2879 2581 1709 1280 1149 1064 374 146
138 30
EcoICRI GAG'CTC
Cuts at: 5992 6047 5992
Size: 55 11295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2032 2321 2335 2680 2727 2752 3240
Size: 393 400 289 14 345 47 25 488
Cuts at: 3240 3255 3563 3827 3885 4055 4281 4447 4738
Size: 15 308 264 58 170 226 166 291
Cuts at: 4738 4813 4947 5576 5627 5916 6020 6259 6887
Size: 75 134 629 51 289 104 239 628
Cuts at: 6887 7181 7217 7391 7454 7945 8405 9413 10010
Size: 294 36 174 63 491 460 1008 597
Cuts at: 10010 10797 11065 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1008 787 629 628 597 491 488
460 400 393 345 308 294 291 289
289 268 264 239 226 174 170 166
134 104 75 63 58 51 47 36
25 15 14
EcoO109I rG'GnC_Cy
Cuts at: 5601 6325 6506 6507 7429 8137 5601
Size: 724 181 1 922 708 8814
Fragments arranged by size:
8814 922 724 708 181 1
EcoRI G'AATT_C
Cuts at: 4027 4214 7439 8659 9037 4027
Size: 187 3225 1220 378 6340
Fragments arranged by size:
6340 3225 1220 378 187
EcoRV GAT'ATC
Cuts at: 5740 5740
Size: 11350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2324 5548 8029 2324
Size: 3224 2481 5645
Fragments arranged by size:
5645 3224 2481
FspI TGC'GCA
Cuts at: 5141 5774 9856 5141
Size: 633 4082 6635
Fragments arranged by size:
6635 4082 633
HaeII r_GCGC'y
Cuts at: 1678 4661 10497 1678
Size: 2983 5836 2531
Fragments arranged by size:
5836 2983 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2346 2378 2614 2646 2932
Size: 32 135 32 774 32 236 32 286
Cuts at: 2932 2964 3820 3852 4351 4383 5724 5756 6109
Size: 32 856 32 499 32 1341 32 353
Cuts at: 6109 6141 6625 6657 7519 7551 7588 7620 7904
Size: 32 484 32 862 32 37 32 284
Cuts at: 7904 7936 8494 8526 8619 8651 9991 10023 10065
Size: 32 558 32 93 32 1340 32 42
Cuts at: 10065 10097 1373
Size: 32 2626
Fragments arranged by size:
2626 1341 1340 862 856 774 558 499
484 353 286 284 236 135 93 42
37 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2410 2679 3713 5197 5506 6055
Size: 988 281 472 269 1034 1484 309 549
Cuts at: 6055 7078 8694 8940 669
Size: 1023 1616 246 3079
Fragments arranged by size:
3079 1616 1484 1034 1023 988 549 472
309 281 269 246
HindIII A'AGCT_T
Cuts at: 748 7298 748
Size: 6550 4800
HpaI GTT'AAC
Cuts at: 8940 8940
Size: 11350
KasI G'GCGC_C
Cuts at: 4657 4657
Size: 11350
KpnI G_GTAC'C
Cuts at: 6922 8675 6922
Size: 1753 9597
MfeI C'AATT_G
Cuts at: 8949 8949
Size: 11350
MluI A'CGCG_T
Cuts at: 11226 11226
Size: 11350
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2244 7779 10464 10648 1305
Size: 939 5535 2685 184 2007
Fragments arranged by size:
5535 2685 2007 939 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2213 4346 6491 8107 8263
Size: 54 1425 343 381 2133 2145 1616 156
Cuts at: 8263 10
Size: 3097
Fragments arranged by size:
3097 2145 2133 1616 1425 381 343 156
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2278 2893 3393 3467 3926 4092 4434
Size: 648 1112 615 500 74 459 166 342
Cuts at: 4434 6896 6935 7023 7025 7127 7223 7556 7886
Size: 2462 39 88 2 102 96 333 330
Cuts at: 7886 8054 8360 8370 8532 9308 9667 9826 518
Size: 168 306 10 162 776 359 159 2042
Fragments arranged by size:
2462 2042 1112 776 648 615 500 459
359 342 333 330 306 168 166 162
159 102 96 88 74 39 10 2
MspA1I CmG'CkG
Cuts at: 1606 2232 2262 3212 3735 5215 5598 5918 5970
Size: 626 30 950 523 1480 383 320 52
Cuts at: 5970 5997 6422 7086 8111 8766 9443 10591 10836
Size: 27 425 664 1025 655 677 1148 245
Cuts at: 10836 1606
Size: 2120
Fragments arranged by size:
2120 1480 1148 1025 950 677 664 655
626 523 425 383 320 245 52 30
27
NaeI GCC'GGC
Cuts at: 6749 6949 6749
Size: 200 11150
NarI GG'CG_CC
Cuts at: 4658 4658
Size: 11350
NcoI C'CATG_G
Cuts at: 513 1065 2004 3328 3525 7600 8269 513
Size: 552 939 1324 197 4075 669 3594
Fragments arranged by size:
4075 3594 1324 939 669 552 197
NdeI CA'TA_TG
Cuts at: 387 3082 387
Size: 2695 8655
NgoMIV G'CCGG_C
Cuts at: 6747 6947 6747
Size: 200 11150
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11350
NotI GC'GGCC_GC
Cuts at: 8797 8797
Size: 11350
NsiI A_TGCA'T
Cuts at: 5677 5677
Size: 11350
NspI r_CATG'y
Cuts at: 1132 1229 3047 3413 4091 4343 4922 5168 7555
Size: 97 1818 366 678 252 579 246 2387
Cuts at: 7555 8651 8708 10253 1132
Size: 1096 57 1545 2229
Fragments arranged by size:
2387 2229 1818 1545 1096 678 579 366
252 246 97 57
PasI CC'CwG_GG
Cuts at: 1348 2845 1348
Size: 1497 9853
PciI A'CATG_T
Cuts at: 10249 10249
Size: 11350
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5554 6251 6498 6701 8270 1108
Size: 4446 697 247 203 1569 4188
Fragments arranged by size:
4446 4188 1569 697 247 203
PfoI T'CCnGG_A
Cuts at: 3598 6036 6358 6674 8187 3598
Size: 2438 322 316 1513 6761
Fragments arranged by size:
6761 2438 1513 322 316
PmeI GTTT'AAAC
Cuts at: 8654 8654
Size: 11350
PmlI CAC'GTG
Cuts at: 6498 6498
Size: 11350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5528 5560 9419 9451 10932 10964 5528
Size: 32 3859 32 1481 32 5914
Fragments arranged by size:
5914 3859 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5601 6325 7429 8137 5601
Size: 724 1104 708 8814
Fragments arranged by size:
8814 1104 724 708
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 11350
PsiI TTA'TAA
Cuts at: 8920 8920
Size: 11350
PspOMI G'GGCC_C
Cuts at: 6506 8351 6506
Size: 1845 9505
PstI C_TGCA'G
Cuts at: 830 2596 3484 6546 7085 7586 8151 8372 8702
Size: 1766 888 3062 539 501 565 221 330
Cuts at: 8702 830
Size: 3478
Fragments arranged by size:
3478 3062 1766 888 565 539 501 330
221
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11350
PvuII CAG'CTG
Cuts at: 3212 3735 5215 5918 5997 7086 3212
Size: 523 1480 703 79 1089 7476
Fragments arranged by size:
7476 1480 1089 703 523 79
SacI G_AGCT'C
Cuts at: 5994 6049 5994
Size: 55 11295
SacII CC_GC'GG
Cuts at: 2233 2233
Size: 11350
SalI G'TCGA_C
Cuts at: 1936 2677 3711 5195 8692 1936
Size: 741 1034 1484 3497 4594
Fragments arranged by size:
4594 3497 1484 1034 741
SanDI GG'GwC_CC
Cuts at: 5601 5601
Size: 11350
SapI GCTCTTCn'nnn_
Cuts at: 5225 5225
Size: 11350
SbfI CC_TGCA'GG
Cuts at: 8702 8702
Size: 11350
ScaI AGT'ACT
Cuts at: 3145 9598 11310 3145
Size: 6453 1712 3185
Fragments arranged by size:
6453 3185 1712
SexAI A'CCwGG_T
Cuts at: 4230 6921 4230
Size: 2691 8659
SfcI C'TryA_G
Cuts at: 826 1045 2592 2602 2830 3480 3496 4128 4257
Size: 219 1547 10 228 650 16 632 129
Cuts at: 4257 6542 7081 7387 7582 7742 8147 8368 8698
Size: 2285 539 306 195 160 405 221 330
Cuts at: 8698 9833 10514 10705 826
Size: 1135 681 191 1471
Fragments arranged by size:
2285 1547 1471 1135 681 650 632 539
405 330 306 228 221 219 195 191
160 129 16 10
SfoI GGC'GCC
Cuts at: 4659 4659
Size: 11350
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11350
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11350
SmaI CCC'GGG
Cuts at: 1872 8343 8677 1872
Size: 6471 334 4545
Fragments arranged by size:
6471 4545 334
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2309 4496 6048 9454 10355 10617
Size: 197 926 366 2187 1552 3406 901 262
Cuts at: 10617 820
Size: 1553
Fragments arranged by size:
3406 2187 1553 1552 926 901 366 262
197
SnaBI TAC'GTA
Cuts at: 493 9141 493
Size: 8648 2702
SpeI A'CTAG_T
Cuts at: 152 3508 6061 152
Size: 3356 2553 5441
Fragments arranged by size:
5441 3356 2553
SphI G_CATG'C
Cuts at: 1132 4922 7555 8708 1132
Size: 3790 2633 1153 3774
Fragments arranged by size:
3790 3774 2633 1153
SspI AAT'ATT
Cuts at: 5 52 3199 5395 9274 5
Size: 47 3147 2196 3879 2081
Fragments arranged by size:
3879 3147 2196 2081 47
StuI AGG'CCT
Cuts at: 1517 2576 2765 5871 6351 1517
Size: 1059 189 3106 480 6516
Fragments arranged by size:
6516 3106 1059 480 189
StyI C'CwwG_G
Cuts at: 513 1065 2004 2760 3328 3525 3671 4042 4351
Size: 552 939 756 568 197 146 371 309
Cuts at: 4351 5653 6486 7321 7600 7770 8004 8269 8789
Size: 1302 833 835 279 170 234 265 520
Cuts at: 8789 513
Size: 3074
Fragments arranged by size:
3074 1302 939 835 833 756 568 552
520 371 309 279 265 234 197 170
146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4903 6370 6854 8303 8427 9377 9394
Size: 1255 3323 1467 484 1449 124 950 17
Cuts at: 9394 9547 9732 10177 325
Size: 153 185 445 1498
Fragments arranged by size:
3323 1498 1467 1449 1255 950 484 445
185 153 124 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3143 4512 5043
Size: 274 80 33 51 1443 1166 1369 531
Cuts at: 5043 5341 6015 6105 7200 9596 11308 96
Size: 298 674 90 1095 2396 1712 138
Fragments arranged by size:
2396 1712 1443 1369 1166 1095 674 531
298 274 138 90 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3246 3278 4299 4331 4593 4625 5434 5466 6597
Size: 32 1021 32 262 32 809 32 1131
Cuts at: 6597 6629 7199 7231 11310 11342 3246
Size: 32 570 32 4079 32 3254
Fragments arranged by size:
4079 3254 1131 1021 809 570 262 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4856 5173 1316
Size: 3540 317 7493
Fragments arranged by size:
7493 3540 317
XbaI T'CTAG_A
Cuts at: 8686 8686
Size: 11350
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 8443 8443
Size: 11350
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11350
XmaI C'CCGG_G
Cuts at: 1870 8341 8675 1870
Size: 6471 334 4545
Fragments arranged by size:
6471 4545 334
XmnI GAAnn'nnTTC
Cuts at: 2064 3229 7118 7439 9479 2064
Size: 1165 3889 321 2040 3935
Fragments arranged by size:
3935 3889 2040 1165 321
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9158 276
Size: 53 83 186 936 7624 2468
Fragments arranged by size:
7624 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BsiWI BstZ17I DraIII
EcoNI FseI FspAI NruI PacI PspXI PsrI RsrII
SfiI SrfI SwaI