Restriction map for
pFN21AB9480
(Circular) (Six-base) MAPSORT of: pFN21AB9480.seq Check: 376 from: 1 to: 7201
pFN21AB9480 7201 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2980 3176 3932 2980
Size: 196 756 6249
Fragments arranged by size:
6249 756 196
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5011 278
Size: 53 83 186 936 3475 2468
Fragments arranged by size:
3475 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2664 2677 3489 3978 4544 1937
Size: 727 13 812 489 566 4594
Fragments arranged by size:
4594 812 727 566 489 13
Acc65I G'GTAC_C
Cuts at: 4522 4522
Size: 7201
AclI AA'CG_TT
Cuts at: 1563 5328 5701 1563
Size: 3765 373 3063
Fragments arranged by size:
3765 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 2386 3053 3152 3986 5264 6648 6916
Size: 642 112 667 99 834 1278 1384 268
Cuts at: 6916 1632
Size: 1917
Fragments arranged by size:
1917 1384 1278 834 667 642 268 112
99
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7004
AflIII A'CryG_T
Cuts at: 1203 3144 6100 7077 1203
Size: 1941 2956 977 1327
Fragments arranged by size:
2956 1941 1327 977
AgeI A'CCGG_T
Cuts at: 2316 6032 2316
Size: 3716 3485
AhdI GACnn_n'nnGTC
Cuts at: 4459 5930 4459
Size: 1471 5730
AleI CACnn'nnGTG
Cuts at: 1166 4145 1166
Size: 2979 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2117 2540 1184
Size: 933 423 5845
Fragments arranged by size:
5845 933 423
AlwNI CAG_nnn'CTG
Cuts at: 2110 3031 4253 4330 4944 6516 2110
Size: 921 1222 77 614 1572 2795
Fragments arranged by size:
2795 1572 1222 921 614 77
ApaLI G'TGCA_C
Cuts at: 2450 5258 6414 2450
Size: 2808 1156 3237
Fragments arranged by size:
3237 2808 1156
ApoI r'AATT_y
Cuts at: 1493 3647 4510 4736 4888 6997 1493
Size: 2154 863 226 152 2109 1697
Fragments arranged by size:
2154 2109 1697 863 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5755 160
Size: 872 4723 1606
Fragments arranged by size:
4723 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
AvaI C'yCGr_G
Cuts at: 1870 1943 4036 4526 1870
Size: 73 2093 490 4545
Fragments arranged by size:
4545 2093 490 73
BamHI G'GATC_C
Cuts at: 4531 4898 4531
Size: 367 6834
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 3701 4522 5977 618
Size: 325 217 627 246 1668 821 1455 1842
Fragments arranged by size:
1842 1668 1455 821 627 325 246 217
BanII G_rGCy'C
Cuts at: 1391 3951 4369 4379 4392 4520 1391
Size: 2560 418 10 13 128 4072
Fragments arranged by size:
4072 2560 418 128 13 10
BbeI G_GCGC'C
Cuts at: 3705 3705
Size: 7201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3045 3886 928
Size: 971 1146 841 4243
Fragments arranged by size:
4243 1146 971 841
BbvCI CC'TCA_GC
Cuts at: 3657 3730 3657
Size: 73 7128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2794 2828 5392 5426 1903
Size: 34 50 34 773 34 2564 34 3678
Fragments arranged by size:
3678 2564 773 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5093 6303 5093
Size: 1210 5991
BclI T'GATC_A
Cuts at: 1548 2934 1548
Size: 1386 5815
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3946 5812 136
Size: 107 122 71 631 605 2274 1866 1525
Fragments arranged by size:
2274 1866 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7196 7196
Size: 7201
BlpI GC'TnA_GC
Cuts at: 4618 4618
Size: 7201
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2454 3942 4219 5262 6418 1790
Size: 246 418 1488 277 1043 1156 2573
Fragments arranged by size:
2573 1488 1156 1043 418 277 246
BmgBI CAC'GTC
Cuts at: 1318 4131 4186 1318
Size: 2813 55 4333
Fragments arranged by size:
4333 2813 55
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2039 3271 3427
Size: 416 53 83 399 24 622 1232 156
Cuts at: 3427 4932 5890 442
Size: 1505 958 1753
Fragments arranged by size:
1753 1505 1232 958 622 416 399 156
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3289 3289
Size: 7201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2326 2573 2891 3041 3339 5861 1239
Size: 1087 247 318 150 298 2522 2579
Fragments arranged by size:
2579 2522 1087 318 298 247 150
Bpu10I CC'TnA_GC
Cuts at: 3657 3730 4323 4378 3657
Size: 73 593 55 6480
Fragments arranged by size:
6480 593 73 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5326 6191 6489 5326
Size: 865 298 6038
Fragments arranged by size:
6038 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5864 882
Size: 617 4365 2219
Fragments arranged by size:
4365 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4992 493
Size: 711 3788 2702
Fragments arranged by size:
3788 2702 711
BsaBI GATnn'nnATC
Cuts at: 7195 7195
Size: 7201
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2502 3702 4437
Size: 53 83 186 936 591 378 1200 735
Cuts at: 4437 5008 5390 6025 275
Size: 571 382 635 1451
Fragments arranged by size:
1451 1200 936 735 635 591 571 382
378 186 83 53
BsaWI w'CCGG_w
Cuts at: 2190 2316 5634 6032 6306 6453 6864 2190
Size: 126 3318 398 274 147 411 2527
Fragments arranged by size:
3318 2527 411 398 274 147 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6976
Fragments arranged by size:
6976 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3326 4430 1186
Size: 2140 1104 3957
Fragments arranged by size:
3957 2140 1104
BseYI C'CCAG_C
Cuts at: 3598 6404 6900 3598
Size: 2806 496 3899
Fragments arranged by size:
3899 2806 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2609 2788 3148 3194 1163
Size: 573 873 179 360 46 5170
Fragments arranged by size:
5170 873 573 360 179 46
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3816 4651 5412 6440 1540
Size: 275 186 1815 835 761 1028 2301
Fragments arranged by size:
2301 1815 1028 835 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2454 3270 4239 4379 5262 5347 6418 2454
Size: 816 969 140 883 85 1071 3237
Fragments arranged by size:
3237 1071 969 883 816 140 85
BsmI GAATG_Cn'
Cuts at: 4712 4805 4712
Size: 93 7108
BsmBI CGTCTCn'nnnn_
Cuts at: 2333 2333
Size: 7201
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2454 3270 3942 3951 4219 4239
Size: 399 246 418 816 672 9 268 20
Cuts at: 4239 4369 4379 4392 4520 5262 5347 6418 1391
Size: 130 10 13 128 742 85 1071 2174
Fragments arranged by size:
2174 1071 816 742 672 418 399 268
246 130 128 85 20 13 10 9
BspEI T'CCGG_A
Cuts at: 2190 6864 2190
Size: 4674 2527
BspHI T'CATG_A
Cuts at: 3906 5090 6820 3906
Size: 1184 1730 4287
Fragments arranged by size:
4287 1730 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2556 2622 2980 3176 3765 3932 4014 4556
Size: 1712 66 358 196 589 167 82 542
Cuts at: 4556 844
Size: 3489
Fragments arranged by size:
3489 1712 589 542 358 196 167 82
66
BsrBI CCG'CTC
Cuts at: 1394 1823 2881 5088 1394
Size: 429 1058 2207 3507
Fragments arranged by size:
3507 2207 1058 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2586 3723 4276 5696 5870 65
Size: 1183 1338 1137 553 1420 174 1396
Fragments arranged by size:
1420 1396 1338 1183 1137 553 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2316 4009 5845 6032 1700
Size: 110 506 1693 1836 187 2869
Fragments arranged by size:
2869 1836 1693 506 187 110
BsrGI T'GTAC_A
Cuts at: 96 3783 3954 96
Size: 3687 171 3343
Fragments arranged by size:
3687 3343 171
BssHII G'CGCG_C
Cuts at: 1925 3806 1925
Size: 1881 5320
BssSI C'ACGA_G
Cuts at: 5261 6273 5261
Size: 1012 6189
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2110 794
Size: 1316 5885
BstBI TT'CG_AA
Cuts at: 6005 6005
Size: 7201
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7201
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 3403 2137
Size: 1266 5935
BstYI r'GATC_y
Cuts at: 1972 2421 4029 4531 4898 5283 5300 6752 6838
Size: 449 1608 502 367 385 17 1452 86
Cuts at: 6838 6850 7196 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1608 1452 502 449 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3172 3375 3750 513
Size: 552 2107 203 375 3964
Fragments arranged by size:
3964 2107 552 375 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2457 4303 487
Size: 683 56 1231 1846 3385
Fragments arranged by size:
3385 1846 1231 683 56
BtsI GCAGTG_nn'
Cuts at: 2165 4284 4717 5511 5531 2165
Size: 2119 433 794 20 3835
Fragments arranged by size:
3835 2119 794 433 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7201
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2932 2967 529
Size: 35 2368 35 4763
Fragments arranged by size:
4763 2368 35 35
DraI TTT'AAA
Cuts at: 4505 4505
Size: 7201
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6208 809
Size: 507 4892 1802
Fragments arranged by size:
4892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2022 2953 3813
Size: 54 1425 50 275 18 192 931 860
Cuts at: 3813 4011 4059 4648 5537 7166 8
Size: 198 48 589 889 1629 43
Fragments arranged by size:
1629 1425 931 889 860 589 275 198
192 54 50 48 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3813 4648 1537
Size: 275 2001 835 4090
Fragments arranged by size:
4090 2001 835 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2735 3332 3344 3626 4085 4097 5131 1353
Size: 1382 597 12 282 459 12 1034 3423
Fragments arranged by size:
3423 1382 1034 597 459 282 12 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5788 6162 6308 387
Size: 1064 138 4199 374 146 1280
Fragments arranged by size:
4199 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4377 4377
Size: 7201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2274 2326 2386 2573 2891 3041 3053
Size: 393 642 52 60 187 318 150 12
Cuts at: 3053 3152 3339 3986 5264 5861 6648 6916 1239
Size: 99 187 647 1278 597 787 268 1524
Fragments arranged by size:
1524 1278 787 647 642 597 393 318
268 187 187 150 99 60 52 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2192 2192
Size: 7201
EcoO109I rG'GnC_Cy
Cuts at: 2107 2545 2107
Size: 438 6763
EcoRI G'AATT_C
Cuts at: 4510 4888 4510
Size: 378 6823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2911 3667 2911
Size: 756 6445
FspI TGC'GCA
Cuts at: 3764 4109 5707 3764
Size: 345 1598 5258
Fragments arranged by size:
5258 1598 345
FspAI rTGC'GCAy
Cuts at: 4109 4109
Size: 7201
HaeII r_GCGC'y
Cuts at: 1678 2082 3705 6348 1678
Size: 404 1623 2643 2531
Fragments arranged by size:
2643 2531 1623 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3273 3305 4379 4411 4445
Size: 32 135 32 1701 32 1074 32 34
Cuts at: 4445 4477 5842 5874 5916 5948 1373
Size: 32 1365 32 42 32 2626
Fragments arranged by size:
2626 1701 1365 1074 135 42 34 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2353 2533 4545 4791 669
Size: 988 281 415 180 2012 246 3079
Fragments arranged by size:
3079 2012 988 415 281 246 180
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7201
HpaI GTT'AAC
Cuts at: 4791 4791
Size: 7201
KasI G'GCGC_C
Cuts at: 3701 3701
Size: 7201
KpnI G_GTAC'C
Cuts at: 4526 4526
Size: 7201
MfeI C'AATT_G
Cuts at: 4800 4800
Size: 7201
MluI A'CGCG_T
Cuts at: 7077 7077
Size: 7201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3243 6315 6499 1305
Size: 1938 3072 184 2007
Fragments arranged by size:
3072 2007 1938 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2024 2955 10
Size: 54 1425 343 192 931 4256
Fragments arranged by size:
4256 1425 931 343 192 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3374 3575 3785 3935 3956 4133 4145
Size: 648 2208 201 210 150 21 177 12
Cuts at: 4145 5159 5518 5677 518
Size: 1014 359 159 2042
Fragments arranged by size:
2208 2042 1014 648 359 210 201 177
159 150 21 12
MspA1I CmG'CkG
Cuts at: 1606 2470 3630 4617 5294 6442 6687 1606
Size: 864 1160 987 677 1148 245 2120
Fragments arranged by size:
2120 1160 1148 987 864 677 245
NaeI GCC'GGC
Cuts at: 4011 4011
Size: 7201
NarI GG'CG_CC
Cuts at: 3702 3702
Size: 7201
NcoI C'CATG_G
Cuts at: 513 1065 3375 3750 513
Size: 552 2310 375 3964
Fragments arranged by size:
3964 2310 552 375
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7201
NgoMIV G'CCGG_C
Cuts at: 4009 4009
Size: 7201
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7201
NotI GC'GGCC_GC
Cuts at: 4648 4648
Size: 7201
NspI r_CATG'y
Cuts at: 1132 1229 2674 3961 4559 6104 1132
Size: 97 1445 1287 598 1545 2229
Fragments arranged by size:
2229 1545 1445 1287 598 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7201
PciI A'CATG_T
Cuts at: 6100 6100
Size: 7201
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4057 1108
Size: 2949 4252
PfoI T'CCnGG_A
Cuts at: 2424 2424
Size: 7201
PmeI GTTT'AAAC
Cuts at: 4505 4505
Size: 7201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3668 3700 5270 5302 6783 6815 3668
Size: 32 1570 32 1481 32 4054
Fragments arranged by size:
4054 1570 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2545 2545
Size: 7201
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7201
PsiI TTA'TAA
Cuts at: 4771 4771
Size: 7201
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2549 2581 2549
Size: 32 7169
PstI C_TGCA'G
Cuts at: 830 2106 3200 4335 4553 830
Size: 1276 1094 1135 218 3478
Fragments arranged by size:
3478 1276 1135 1094 218
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7201
SacI G_AGCT'C
Cuts at: 4379 4379
Size: 7201
SalI G'TCGA_C
Cuts at: 1936 4543 1936
Size: 2607 4594
SapI GCTCTTCn'nnn_
Cuts at: 4097 4097
Size: 7201
SbfI CC_TGCA'GG
Cuts at: 2106 4553 2106
Size: 2447 4754
ScaI AGT'ACT
Cuts at: 3008 5449 7161 3008
Size: 2441 1712 3048
Fragments arranged by size:
3048 2441 1712
SexAI A'CCwGG_T
Cuts at: 3972 4050 4497 3972
Size: 78 447 6676
Fragments arranged by size:
6676 447 78
SfcI C'TryA_G
Cuts at: 826 1045 2074 2102 2566 2665 3196 3913 4331
Size: 219 1029 28 464 99 531 717 418
Cuts at: 4331 4549 5684 6365 6556 826
Size: 218 1135 681 191 1471
Fragments arranged by size:
1471 1135 1029 717 681 531 464 418
219 218 191 99 28
SfoI GGC'GCC
Cuts at: 3703 3703
Size: 7201
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7201
SmaI CCC'GGG
Cuts at: 1872 4528 1872
Size: 2656 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5305 6206 6468 820
Size: 197 926 3362 901 262 1553
Fragments arranged by size:
3362 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4992 493
Size: 4499 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7201
SphI G_CATG'C
Cuts at: 1132 2674 4559 1132
Size: 1542 1885 3774
Fragments arranged by size:
3774 1885 1542
SspI AAT'ATT
Cuts at: 5 52 5125 5
Size: 47 5073 2081
Fragments arranged by size:
5073 2081 47
StuI AGG'CCT
Cuts at: 1517 3774 3848 1517
Size: 2257 74 4870
Fragments arranged by size:
4870 2257 74
StyI C'CwwG_G
Cuts at: 513 1065 2061 2223 3375 3750 4640 513
Size: 552 996 162 1152 375 890 3074
Fragments arranged by size:
3074 1152 996 890 552 375 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2280 3446 5228 5245 5398 5583 6028
Size: 1255 700 1166 1782 17 153 185 445
Cuts at: 6028 325
Size: 1498
Fragments arranged by size:
1782 1498 1255 1166 700 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2892 3006 3783
Size: 274 80 33 51 1443 915 114 777
Cuts at: 3783 3954 5447 7159 96
Size: 171 1493 1712 138
Fragments arranged by size:
1712 1493 1443 915 777 274 171 138
114 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2444 2476 7161 7193 2444
Size: 32 4685 32 2452
Fragments arranged by size:
4685 2452 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3229 1316
Size: 1913 5288
XbaI T'CTAG_A
Cuts at: 4537 4537
Size: 7201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2369 4190 2369
Size: 1821 5380
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7201
XmaI C'CCGG_G
Cuts at: 1870 4526 1870
Size: 2656 4545
XmnI GAAnn'nnTTC
Cuts at: 3137 5330 3137
Size: 2193 5008
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5009 276
Size: 53 83 186 936 3475 2468
Fragments arranged by size:
3475 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I DraIII EcoRV FseI NruI NsiI PacI
PmlI PspOMI PspXI PvuII RsrII SacII SanDI SfiI
SrfI SwaI