Restriction map for
pFN21AB9479
(Circular) (Six-base) MAPSORT of: pFN21AB9479.seq Check: 3978 from: 1 to: 6898
pFN21AB9479 6898 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3931 3931
Size: 6898
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4708 278
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4241 1937
Size: 2304 4594
Acc65I G'GTAC_C
Cuts at: 4219 4219
Size: 6898
AclI AA'CG_TT
Cuts at: 1563 5025 5398 1563
Size: 3462 373 3063
Fragments arranged by size:
3462 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2876 3167 4054 4118 4961 6345 6613 1632
Size: 1244 291 887 64 843 1384 268 1917
Fragments arranged by size:
1917 1384 1244 887 843 291 268 64
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6701
AflIII A'CryG_T
Cuts at: 1203 2592 5797 6774 1203
Size: 1389 3205 977 1327
Fragments arranged by size:
3205 1389 1327 977
AgeI A'CCGG_T
Cuts at: 5729 5729
Size: 6898
AhdI GACnn_n'nnGTC
Cuts at: 5627 5627
Size: 6898
AleI CACnn'nnGTG
Cuts at: 1166 2333 2462 1166
Size: 1167 129 5602
Fragments arranged by size:
5602 1167 129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6898
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3126 3158 3126
Size: 32 6866
AlwNI CAG_nnn'CTG
Cuts at: 2024 2105 2365 2392 2663 2899 3004 3025 3238
Size: 81 260 27 271 236 105 21 213
Cuts at: 3238 3322 3331 3847 4641 6213 2024
Size: 84 9 516 794 1572 2709
Fragments arranged by size:
2709 1572 794 516 271 260 236 213
105 84 81 27 21 9
ApaI G_GGCC'C
Cuts at: 2081 2611 2676 3013 3050 3617 4155 2081
Size: 530 65 337 37 567 538 4824
Fragments arranged by size:
4824 567 538 530 337 65 37
ApaLI G'TGCA_C
Cuts at: 4955 6111 4955
Size: 1156 5742
ApoI r'AATT_y
Cuts at: 1493 3406 4207 4433 4585 6694 1493
Size: 1913 801 226 152 2109 1697
Fragments arranged by size:
2109 1913 1697 801 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5452 160
Size: 872 4420 1606
Fragments arranged by size:
4420 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
AvaI C'yCGr_G
Cuts at: 1870 1943 2711 4223 1870
Size: 73 768 1512 4545
Fragments arranged by size:
4545 1512 768 73
BamHI G'GATC_C
Cuts at: 4228 4595 4228
Size: 367 6531
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2524 3384 3754 4219
Size: 325 217 627 462 275 860 370 465
Cuts at: 4219 5674 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 860 627 465 462 370 325
275 217
BanII G_rGCy'C
Cuts at: 1391 2081 2116 2381 2611 2676 2953 2983 3013
Size: 690 35 265 230 65 277 30 30
Cuts at: 3013 3050 3617 4139 4155 4217 1391
Size: 37 567 522 16 62 4072
Fragments arranged by size:
4072 690 567 522 277 265 230 65
62 37 35 30 30 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2804 928
Size: 971 905 5022
Fragments arranged by size:
5022 971 905
BbvCI CC'TCA_GC
Cuts at: 2514 3171 2514
Size: 657 6241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5089 5123 1903
Size: 34 3152 34 3678
Fragments arranged by size:
3678 3152 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4790 6000 4790
Size: 1210 5688
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6898
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2120 2917 3377
Size: 107 122 71 631 605 448 797 460
Cuts at: 3377 5509 136
Size: 2132 1525
Fragments arranged by size:
2132 1525 797 631 605 460 448 122
107 71
BglII A'GATC_T
Cuts at: 6893 6893
Size: 6898
BlpI GC'TnA_GC
Cuts at: 3294 3465 4315 3294
Size: 171 850 5877
Fragments arranged by size:
5877 850 171
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2252 2529 2611 2676 3013 3050 3617
Size: 291 171 277 82 65 337 37 567
Cuts at: 3617 3757 3790 4155 4167 4959 6115 1790
Size: 140 33 365 12 792 1156 2573
Fragments arranged by size:
2573 1156 792 567 365 337 291 277
171 140 82 65 37 33 12
BmgBI CAC'GTC
Cuts at: 1318 2464 1318
Size: 1146 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2084 2679 2765
Size: 416 53 83 399 24 667 595 86
Cuts at: 2765 3349 3526 3824 4629 5587 442
Size: 584 177 298 805 958 1753
Fragments arranged by size:
1753 958 805 667 595 584 416 399
298 177 86 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3827 1056
Size: 2771 4127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3868 3868
Size: 6898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2425 3002 3005 3047 3185 3260 3353 3678
Size: 1186 577 3 42 138 75 93 325
Cuts at: 3678 4013 5558 1239
Size: 335 1545 2579
Fragments arranged by size:
2579 1545 1186 577 335 325 138 93
75 42 3
Bpu10I CC'TnA_GC
Cuts at: 2514 2750 3171 3769 2514
Size: 236 421 598 5643
Fragments arranged by size:
5643 598 421 236
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5023 5888 6186 5023
Size: 865 298 5735
Fragments arranged by size:
5735 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2307 2318 3364 3447 5561 882
Size: 617 808 11 1046 83 2114 2219
Fragments arranged by size:
2219 2114 1046 808 617 83 11
BsaAI yAC'GTr
Cuts at: 493 1204 4689 493
Size: 711 3485 2702
Fragments arranged by size:
3485 2702 711
BsaBI GATnn'nnATC
Cuts at: 6892 6892
Size: 6898
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4705 5087 5722 275
Size: 53 83 186 936 3172 382 635 1451
Fragments arranged by size:
3172 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3456 5331 5729 6003 6150 6561 3456
Size: 1875 398 274 147 411 3793
Fragments arranged by size:
3793 1875 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2120 2150 2286 2316 1178
Size: 30 165 30 717 30 136 30 5760
Fragments arranged by size:
5760 717 165 136 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2246 2441 2627 2939 2942 3259 3593 3643
Size: 1060 195 186 312 3 317 334 50
Cuts at: 3643 4022 1186
Size: 379 4062
Fragments arranged by size:
4062 1060 379 334 317 312 195 186
50 3
BseYI C'CCAG_C
Cuts at: 2034 2162 2549 2575 2792 2865 2937 3217 3274
Size: 128 387 26 217 73 72 280 57
Cuts at: 3274 3298 3890 6101 6597 2034
Size: 24 592 2211 496 2335
Fragments arranged by size:
2335 2211 592 496 387 280 217 128
73 72 57 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2583 1163
Size: 573 847 5478
Fragments arranged by size:
5478 847 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4348 5109 6137 1540
Size: 275 186 2347 761 1028 2301
Fragments arranged by size:
2347 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3471 4959 5044 6115 3471
Size: 1488 85 1071 4254
Fragments arranged by size:
4254 1488 1071 85
BsmI GAATG_Cn'
Cuts at: 2220 2938 4409 4502 2220
Size: 718 1471 93 4616
Fragments arranged by size:
4616 1471 718 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2479 2479
Size: 6898
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2116 2252 2381 2529 2611 2676
Size: 399 291 35 136 129 148 82 65
Cuts at: 2676 2953 2983 3013 3050 3471 3617 3757 3790
Size: 277 30 30 37 421 146 140 33
Cuts at: 3790 4139 4155 4167 4217 4959 5044 6115 1391
Size: 349 16 12 50 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 421 399 349 291 277
148 146 140 136 129 85 82 65
50 37 35 33 30 30 16 12
BspEI T'CCGG_A
Cuts at: 6561 6561
Size: 6898
BspHI T'CATG_A
Cuts at: 3955 4787 6517 3955
Size: 832 1730 4336
Fragments arranged by size:
4336 1730 832
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2325 2686 3931 4253 844
Size: 1481 361 1245 322 3489
Fragments arranged by size:
3489 1481 1245 361 322
BsrBI CCG'CTC
Cuts at: 1394 1823 2692 4785 1394
Size: 429 869 2093 3507
Fragments arranged by size:
3507 2093 869 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5393 5567 65
Size: 1183 4145 174 1396
Fragments arranged by size:
4145 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2504 5542 5729 1700
Size: 110 694 3038 187 2869
Fragments arranged by size:
3038 2869 694 187 110
BsrGI T'GTAC_A
Cuts at: 96 3226 96
Size: 3130 3768
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6898
BssSI C'ACGA_G
Cuts at: 4958 5970 4958
Size: 1012 5886
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3004 794
Size: 2210 4688
BstBI TT'CG_AA
Cuts at: 5702 5702
Size: 6898
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6898
BstXI CCAn_nnnn'nTGG
Cuts at: 3353 3656 4082 3353
Size: 303 426 6169
Fragments arranged by size:
6169 426 303
BstYI r'GATC_y
Cuts at: 1972 3235 4228 4595 4980 4997 6449 6535 6547
Size: 1263 993 367 385 17 1452 86 12
Cuts at: 6547 6893 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1263 993 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2169 2331 2520 2676 3265 513
Size: 552 939 165 162 189 156 589 4146
Fragments arranged by size:
4146 939 589 552 189 165 162 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6159
Fragments arranged by size:
6159 683 56
BtsI GCAGTG_nn'
Cuts at: 2452 4414 5208 5228 2452
Size: 1962 794 20 4122
Fragments arranged by size:
4122 1962 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6898
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3662 3697 529
Size: 35 3098 35 3730
Fragments arranged by size:
3730 3098 35 35
DraI TTT'AAA
Cuts at: 4202 4202
Size: 6898
DraIII CAC_nnn'GTG
Cuts at: 3089 3089
Size: 6898
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2303 3356 5905 809
Size: 507 987 1053 2549 1802
Fragments arranged by size:
2549 1802 1053 987 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2829 3496 4345
Size: 54 1425 50 275 18 999 667 849
Cuts at: 4345 5234 6863 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 999 889 849 667 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4345 1537
Size: 275 2533 4090
Fragments arranged by size:
4090 2533 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 2645 2880 4097 4828 1353
Size: 842 450 235 1217 731 3423
Fragments arranged by size:
3423 1217 842 731 450 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5485 5859 6005 387
Size: 1064 138 3896 374 146 1280
Fragments arranged by size:
3896 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2425 2876 3002 3005 3047 3167 3185
Size: 393 793 451 126 3 42 120 18
Cuts at: 3185 3260 3353 3678 4013 4054 4118 4961 5558
Size: 75 93 325 335 41 64 843 597
Cuts at: 5558 6345 6613 1239
Size: 787 268 1524
Fragments arranged by size:
1524 843 793 787 597 451 393 335
325 268 126 120 93 75 64 42
41 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2289 2289
Size: 6898
EcoO109I rG'GnC_Cy
Cuts at: 2122 2362 2607 2608 2632 2698 2767 3046 3113
Size: 240 245 1 24 66 69 279 67
Cuts at: 3113 3271 3328 3349 3613 3799 4151 2122
Size: 158 57 21 264 186 352 4869
Fragments arranged by size:
4869 352 279 264 245 240 186 158
69 67 66 57 24 21 1
EcoRI G'AATT_C
Cuts at: 3406 4207 4585 3406
Size: 801 378 5719
Fragments arranged by size:
5719 801 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3415 3415
Size: 6898
FseI GG_CCGG'CC
Cuts at: 2508 2508
Size: 6898
FspI TGC'GCA
Cuts at: 5404 5404
Size: 6898
HaeII r_GCGC'y
Cuts at: 1678 6045 1678
Size: 4367 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 4112 4144 5539
Size: 32 135 32 515 32 1993 32 1395
Cuts at: 5539 5571 5613 5645 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1993 1395 515 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4242 4488 669
Size: 988 281 2304 246 3079
Fragments arranged by size:
3079 2304 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6898
HpaI GTT'AAC
Cuts at: 4488 4488
Size: 6898
KpnI G_GTAC'C
Cuts at: 4223 4223
Size: 6898
MfeI C'AATT_G
Cuts at: 4497 4497
Size: 6898
MluI A'CGCG_T
Cuts at: 6774 6774
Size: 6898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6012 6196 1305
Size: 4707 184 2007
Fragments arranged by size:
4707 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2831 10
Size: 54 1425 343 999 4077
Fragments arranged by size:
4077 1425 999 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2333 2462 4856 5215 5374 518
Size: 648 1167 129 2394 359 159 2042
Fragments arranged by size:
2394 2042 1167 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2436 2761 2869 2977 3004 3163 3267 3898
Size: 830 325 108 108 27 159 104 631
Cuts at: 3898 4314 4991 6139 6384 1606
Size: 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 830 677 631 416 325 245
159 108 108 104 27
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 6898
NcoI C'CATG_G
Cuts at: 513 1065 2004 2169 2520 2676 513
Size: 552 939 165 351 156 4735
Fragments arranged by size:
4735 939 552 351 165 156
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6898
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 6898
NheI G'CTAG_C
Cuts at: 1052 3823 1052
Size: 2771 4127
NotI GC'GGCC_GC
Cuts at: 4345 4345
Size: 6898
NspI r_CATG'y
Cuts at: 1132 1229 2596 4256 5801 1132
Size: 97 1367 1660 1545 2229
Fragments arranged by size:
2229 1660 1545 1367 97
PasI CC'CwG_GG
Cuts at: 1348 3117 3353 3760 3885 4155 1348
Size: 1769 236 407 125 270 4091
Fragments arranged by size:
4091 1769 407 270 236 125
PciI A'CATG_T
Cuts at: 2592 5797 2592
Size: 3205 3693
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3025 3310 1108
Size: 1917 285 4696
Fragments arranged by size:
4696 1917 285
PfoI T'CCnGG_A
Cuts at: 2087 3972 2087
Size: 1885 5013
PmeI GTTT'AAAC
Cuts at: 4202 4202
Size: 6898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4967 4999 6480 6512 4967
Size: 32 1481 32 5353
Fragments arranged by size:
5353 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2632 2698 3271 3349 2362
Size: 270 66 573 78 5911
Fragments arranged by size:
5911 573 270 78 66
PshAI GACnn'nnGTC
Cuts at: 1613 2240 1613
Size: 627 6271
PsiI TTA'TAA
Cuts at: 4468 4468
Size: 6898
PspOMI G'GGCC_C
Cuts at: 2077 2607 2672 3009 3046 3613 4151 2077
Size: 530 65 337 37 567 538 4824
Fragments arranged by size:
4824 567 538 530 337 65 37
PstI C_TGCA'G
Cuts at: 830 2121 2322 2766 2862 2874 2958 3000 3327
Size: 1291 201 444 96 12 84 42 327
Cuts at: 3327 4250 830
Size: 923 3478
Fragments arranged by size:
3478 1291 923 444 327 201 96 84
42 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6898
PvuII CAG'CTG
Cuts at: 2761 2869 3004 3163 3898 2761
Size: 108 135 159 735 5761
Fragments arranged by size:
5761 735 159 135 108
SacII CC_GC'GG
Cuts at: 3268 3268
Size: 6898
SalI G'TCGA_C
Cuts at: 1936 4240 1936
Size: 2304 4594
SanDI GG'GwC_CC
Cuts at: 3271 3271
Size: 6898
SapI GCTCTTCn'nnn_
Cuts at: 2195 2880 2195
Size: 685 6213
SbfI CC_TGCA'GG
Cuts at: 2121 2322 4250 2121
Size: 201 1928 4769
Fragments arranged by size:
4769 1928 201
ScaI AGT'ACT
Cuts at: 5146 6858 5146
Size: 1712 5186
SfcI C'TryA_G
Cuts at: 826 1045 2117 2318 2762 2858 2870 2954 2996
Size: 219 1072 201 444 96 12 84 42
Cuts at: 2996 3323 4246 5381 6062 6253 826
Size: 327 923 1135 681 191 1471
Fragments arranged by size:
1471 1135 1072 923 681 444 327 219
201 191 96 84 42 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6898
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6898
SmaI CCC'GGG
Cuts at: 1872 4225 1872
Size: 2353 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5002 5903 6165 820
Size: 197 926 3059 901 262 1553
Fragments arranged by size:
3059 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4689 493
Size: 4196 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6898
SphI G_CATG'C
Cuts at: 1132 4256 1132
Size: 3124 3774
SspI AAT'ATT
Cuts at: 5 52 4822 5
Size: 47 4770 2081
Fragments arranged by size:
4770 2081 47
StuI AGG'CCT
Cuts at: 1517 2343 3380 1517
Size: 826 1037 5035
Fragments arranged by size:
5035 1037 826
StyI C'CwwG_G
Cuts at: 513 1065 2004 2169 2520 2602 2676 2832 3569
Size: 552 939 165 351 82 74 156 737
Cuts at: 3569 3629 3903 4337 513
Size: 60 274 434 3074
Fragments arranged by size:
3074 939 737 552 434 351 274 165
156 82 74 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2026 4925 4942 5095 5280 5725 325
Size: 1255 446 2899 17 153 185 445 1498
Fragments arranged by size:
2899 1498 1255 446 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3226 5144 6856
Size: 274 80 33 51 1443 1249 1918 1712
Cuts at: 6856 96
Size: 138
Fragments arranged by size:
1918 1712 1443 1249 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2120 2152 3377 3409 3422 3454 6858 6890 2120
Size: 32 1225 32 13 32 3404 32 2128
Fragments arranged by size:
3404 2128 1225 32 32 32 32 13
Tth111I GACn'n_nGTC
Cuts at: 1316 2786 3038 1316
Size: 1470 252 5176
Fragments arranged by size:
5176 1470 252
XbaI T'CTAG_A
Cuts at: 4234 4234
Size: 6898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2527 2683 3549 2527
Size: 156 866 5876
Fragments arranged by size:
5876 866 156
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6898
XmaI C'CCGG_G
Cuts at: 1870 4223 1870
Size: 2353 4545
XmnI GAAnn'nnTTC
Cuts at: 2889 5027 2889
Size: 2138 4760
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4706 276
Size: 53 83 186 936 3172 2468
Fragments arranged by size:
3172 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
Bsu36I EcoICRI EcoRV FspAI KasI NarI NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI
SfoI SrfI SwaI