Restriction map for
pFN21AB9478
(Circular) (Six-base) MAPSORT of: pFN21AB9478.seq Check: 9738 from: 1 to: 8338
pFN21AB9478 8338 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6148 278
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5058 5681 1937
Size: 3121 623 4594
Fragments arranged by size:
4594 3121 623
Acc65I G'GTAC_C
Cuts at: 5659 5659
Size: 8338
AclI AA'CG_TT
Cuts at: 1563 6465 6838 1563
Size: 4902 373 3063
Fragments arranged by size:
4902 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3038 3104 3269 6401 7785 8053 1632
Size: 1406 66 165 3132 1384 268 1917
Fragments arranged by size:
3132 1917 1406 1384 268 165 66
AflII C'TTAA_G
Cuts at: 820 1017 4649 820
Size: 197 3632 4509
Fragments arranged by size:
4509 3632 197
AflIII A'CryG_T
Cuts at: 1203 2181 7237 8214 1203
Size: 978 5056 977 1327
Fragments arranged by size:
5056 1327 978 977
AgeI A'CCGG_T
Cuts at: 7169 7169
Size: 8338
AhdI GACnn_n'nnGTC
Cuts at: 7067 7067
Size: 8338
AleI CACnn'nnGTG
Cuts at: 1166 2331 5543 1166
Size: 1165 3212 3961
Fragments arranged by size:
3961 3212 1165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8338
AlwNI CAG_nnn'CTG
Cuts at: 2479 5024 5069 6081 7653 2479
Size: 2545 45 1012 1572 3164
Fragments arranged by size:
3164 2545 1572 1012 45
ApaI G_GGCC'C
Cuts at: 3599 3724 4139 3599
Size: 125 415 7798
Fragments arranged by size:
7798 415 125
ApaLI G'TGCA_C
Cuts at: 5548 5585 6395 7551 5548
Size: 37 810 1156 6335
Fragments arranged by size:
6335 1156 810 37
ApoI r'AATT_y
Cuts at: 1493 3244 5084 5519 5647 5873 6025 8134 1493
Size: 1751 1840 435 128 226 152 2109 1697
Fragments arranged by size:
2109 1840 1751 1697 435 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 6892 160
Size: 872 5860 1606
Fragments arranged by size:
5860 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
AvaI C'yCGr_G
Cuts at: 1870 1943 3717 3843 3906 3957 4334 5663 1870
Size: 73 1774 126 63 51 377 1329 4545
Fragments arranged by size:
4545 1774 1329 377 126 73 63 51
BamHI G'GATC_C
Cuts at: 5668 6035 5668
Size: 367 7971
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3624 5272 5659 7114 618
Size: 325 217 627 1837 1648 387 1455 1842
Fragments arranged by size:
1842 1837 1648 1455 627 387 325 217
BanII G_rGCy'C
Cuts at: 1391 2136 3495 3599 3615 3724 3786 4028 4139
Size: 745 1359 104 16 109 62 242 111
Cuts at: 4139 4415 4772 5657 1391
Size: 276 357 885 4072
Fragments arranged by size:
4072 1359 885 745 357 276 242 111
109 104 62 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3282 3947 928
Size: 971 1383 665 5319
Fragments arranged by size:
5319 1383 971 665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6529 6563 1903
Size: 34 4592 34 3678
Fragments arranged by size:
4592 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4314 6230 7440 4314
Size: 1916 1210 5212
Fragments arranged by size:
5212 1916 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8338
BfrBI ATG'CAT
Cuts at: 5157 5286 5157
Size: 129 8209
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3619 3719 4564
Size: 107 122 71 631 605 1947 100 845
Cuts at: 4564 6949 136
Size: 2385 1525
Fragments arranged by size:
2385 1947 1525 845 631 605 122 107
100 71
BglII A'GATC_T
Cuts at: 5074 8333 5074
Size: 3259 5079
BlpI GC'TnA_GC
Cuts at: 3144 5534 5755 3144
Size: 2390 221 5727
Fragments arranged by size:
5727 2390 221
Bme1580I G_kGCm'C
Cuts at: 1790 3599 3724 3996 4139 5552 5589 6399 7555
Size: 1809 125 272 143 1413 37 810 1156
Cuts at: 7555 1790
Size: 2573
Fragments arranged by size:
2573 1809 1413 1156 810 272 143 125
37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2008 2618 5350
Size: 416 53 83 399 24 591 610 2732
Cuts at: 5350 5405 6069 7027 442
Size: 55 664 958 1753
Fragments arranged by size:
2732 1753 958 664 610 591 416 399
83 55 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2568 3688 3985 4511 4546 4756 6998 1239
Size: 1329 1120 297 526 35 210 2242 2579
Fragments arranged by size:
2579 2242 1329 1120 526 297 210 35
Bpu10I CC'TnA_GC
Cuts at: 3519 4098 4259 3519
Size: 579 161 7598
Fragments arranged by size:
7598 579 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2766 4408 4854 6463 7328 7626 2766
Size: 1642 446 1609 865 298 3478
Fragments arranged by size:
3478 1642 1609 865 446 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2547 2768 3903 7001 882
Size: 617 1048 221 1135 3098 2219
Fragments arranged by size:
3098 2219 1135 1048 617 221
BsaAI yAC'GTr
Cuts at: 493 1204 6129 493
Size: 711 4925 2702
Fragments arranged by size:
4925 2702 711
BsaBI GATnn'nnATC
Cuts at: 8332 8332
Size: 8338
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6145 6527 7162 275
Size: 53 83 186 936 4612 382 635 1451
Fragments arranged by size:
4612 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2464 3511 4802 6771 7169 7443 7590 8001 2464
Size: 1047 1291 1969 398 274 147 411 2801
Fragments arranged by size:
2801 1969 1291 1047 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3493 3523 4010 4040 1178
Size: 30 165 30 2090 30 487 30 5476
Fragments arranged by size:
5476 2090 487 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3331 3766 4260 4423 4561 4771 1186
Size: 2145 435 494 163 138 210 4753
Fragments arranged by size:
4753 2145 494 435 210 163 138
BseYI C'CCAG_C
Cuts at: 2728 2805 3505 4326 7541 8037 2728
Size: 77 700 821 3215 496 3029
Fragments arranged by size:
3215 3029 821 700 496 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3333 3429 4629 1163
Size: 573 1597 96 1200 4872
Fragments arranged by size:
4872 1597 1200 573 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5788 6549 7577 1540
Size: 275 186 3787 761 1028 2301
Fragments arranged by size:
3787 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3364 3608 4028 4104 4415 4791 5552 5589 6399
Size: 244 420 76 311 376 761 37 810
Cuts at: 6399 6484 7555 3364
Size: 85 1071 4147
Fragments arranged by size:
4147 1071 810 761 420 376 311 244
85 76 37
BsmI GAATG_Cn'
Cuts at: 2307 3670 5849 5942 2307
Size: 1363 2179 93 4703
Fragments arranged by size:
4703 2179 1363 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 3364 3495 3599 3608 3615 3724
Size: 399 346 1228 131 104 9 7 109
Cuts at: 3724 3786 3996 4028 4104 4139 4415 4772 4791
Size: 62 210 32 76 35 276 357 19
Cuts at: 4791 5552 5589 5657 6399 6484 7555 1391
Size: 761 37 68 742 85 1071 2174
Fragments arranged by size:
2174 1228 1071 761 742 399 357 346
276 210 131 109 104 85 76 68
62 37 35 32 19 9 7
BspEI T'CCGG_A
Cuts at: 3511 4802 8001 3511
Size: 1291 3199 3848
Fragments arranged by size:
3848 3199 1291
BspHI T'CATG_A
Cuts at: 6227 7957 6227
Size: 1730 6608
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2052 3839 4317 5693 844
Size: 1208 1787 478 1376 3489
Fragments arranged by size:
3489 1787 1376 1208 478
BsrBI CCG'CTC
Cuts at: 1394 1823 4258 6225 1394
Size: 429 2435 1967 3507
Fragments arranged by size:
3507 2435 1967 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2865 4223 5286 6833 7007 65
Size: 1183 1617 1358 1063 1547 174 1396
Fragments arranged by size:
1617 1547 1396 1358 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6982 7169 1700
Size: 110 5172 187 2869
Fragments arranged by size:
5172 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2025 96
Size: 1929 6409
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8338
BssSI C'ACGA_G
Cuts at: 3658 5503 6398 7410 3658
Size: 1845 895 1012 4586
Fragments arranged by size:
4586 1845 1012 895
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5163 794
Size: 4369 3969
BstBI TT'CG_AA
Cuts at: 2050 7142 2050
Size: 5092 3246
BstEII G'GTnAC_C
Cuts at: 1184 2534 1184
Size: 1350 6988
BstYI r'GATC_y
Cuts at: 1972 3685 5074 5668 6035 6420 6437 7889 7975
Size: 1713 1389 594 367 385 17 1452 86
Cuts at: 7975 7987 8333 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1713 1452 1389 594 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2874 2892 3617 4185 513
Size: 552 939 870 18 725 568 4666
Fragments arranged by size:
4666 939 870 725 568 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2222 5201 487
Size: 683 56 996 2979 3624
Fragments arranged by size:
3624 2979 996 683 56
BtsI GCAGTG_nn'
Cuts at: 2760 2939 3412 3996 5377 5389 5854 6648 6668
Size: 179 473 584 1381 12 465 794 20
Cuts at: 6668 2760
Size: 4430
Fragments arranged by size:
4430 1381 794 584 473 465 179 20
12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8338
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4266 4301 529
Size: 35 3702 35 4566
Fragments arranged by size:
4566 3702 35 35
DraI TTT'AAA
Cuts at: 5642 5642
Size: 8338
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7345 809
Size: 507 6029 1802
Fragments arranged by size:
6029 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3104 4434 4681
Size: 54 1425 50 275 18 1274 1330 247
Cuts at: 4681 5785 6674 8303 8
Size: 1104 889 1629 43
Fragments arranged by size:
1629 1425 1330 1274 1104 889 275 247
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5785 1537
Size: 275 3973 4090
Fragments arranged by size:
4090 3973 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2536 2670 3069 4400 4738 4747 6268 1353
Size: 1183 134 399 1331 338 9 1521 3423
Fragments arranged by size:
3423 1521 1331 1183 399 338 134 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6925 7299 7445 387
Size: 1064 138 5336 374 146 1280
Fragments arranged by size:
5336 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4026 4413 4026
Size: 387 7951
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2568 3038 3104 3269 3688 3985 4511
Size: 393 936 470 66 165 419 297 526
Cuts at: 4511 4546 4756 6401 6998 7785 8053 1239
Size: 35 210 1645 597 787 268 1524
Fragments arranged by size:
1645 1524 936 787 597 526 470 419
393 297 268 210 165 66 35
EcoNI CCTnn'n_nnAGG
Cuts at: 2295 2483 2498 2295
Size: 188 15 8135
Fragments arranged by size:
8135 188 15
EcoO109I rG'GnC_Cy
Cuts at: 2746 2911 3595 3596 3721 4092 4135 4566 4687
Size: 165 684 1 125 371 43 431 121
Cuts at: 4687 5352 5497 2746
Size: 665 145 5587
Fragments arranged by size:
5587 684 665 431 371 165 145 125
121 43 1
EcoRI G'AATT_C
Cuts at: 5519 5647 6025 5519
Size: 128 378 7832
Fragments arranged by size:
7832 378 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3299 4916 3299
Size: 1617 6721
FspI TGC'GCA
Cuts at: 6844 6844
Size: 8338
HaeII r_GCGC'y
Cuts at: 1678 5126 7485 1678
Size: 3448 2359 2531
Fragments arranged by size:
3448 2531 2359
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2473 2505 3013
Size: 32 135 32 575 32 294 32 508
Cuts at: 3013 3045 3428 3460 3742 3774 4298 4330 6979
Size: 32 383 32 282 32 524 32 2649
Cuts at: 6979 7011 7053 7085 1373
Size: 32 42 32 2626
Fragments arranged by size:
2649 2626 575 524 508 383 294 282
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5682 5928 669
Size: 988 281 3744 246 3079
Fragments arranged by size:
3744 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4282 748
Size: 3534 4804
HpaI GTT'AAC
Cuts at: 5928 5928
Size: 8338
KpnI G_GTAC'C
Cuts at: 5663 5663
Size: 8338
MfeI C'AATT_G
Cuts at: 5937 5937
Size: 8338
MluI A'CGCG_T
Cuts at: 8214 8214
Size: 8338
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2676 3551 3784 4939 7452 7636 1305
Size: 1371 875 233 1155 2513 184 2007
Fragments arranged by size:
2513 2007 1371 1155 875 233 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4436 4683 10
Size: 54 1425 343 2604 247 3665
Fragments arranged by size:
3665 2604 1425 343 247 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2331 5186 5543 6296 6655 6814 518
Size: 648 1165 2855 357 753 359 159 2042
Fragments arranged by size:
2855 2042 1165 753 648 359 357 159
MspA1I CmG'CkG
Cuts at: 1606 3143 3547 3581 4155 5754 6431 7579 7824
Size: 1537 404 34 574 1599 677 1148 245
Cuts at: 7824 1606
Size: 2120
Fragments arranged by size:
2120 1599 1537 1148 677 574 404 245
34
NcoI C'CATG_G
Cuts at: 513 1065 2004 2892 513
Size: 552 939 888 5959
Fragments arranged by size:
5959 939 888 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8338
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8338
NotI GC'GGCC_GC
Cuts at: 5785 5785
Size: 8338
NsiI A_TGCA'T
Cuts at: 5159 5288 5159
Size: 129 8209
NspI r_CATG'y
Cuts at: 1132 1229 2185 3188 5161 5696 7241 1132
Size: 97 956 1003 1973 535 1545 2229
Fragments arranged by size:
2229 1973 1545 1003 956 535 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8338
PciI A'CATG_T
Cuts at: 2181 7237 2181
Size: 5056 3282
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4186 1108
Size: 3078 5260
PfoI T'CCnGG_A
Cuts at: 4194 4194
Size: 8338
PmeI GTTT'AAAC
Cuts at: 5642 5642
Size: 8338
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6407 6439 7920 7952 6407
Size: 32 1481 32 6793
Fragments arranged by size:
6793 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2746 4092 4687 5352 2746
Size: 1346 595 665 5732
Fragments arranged by size:
5732 1346 665 595
PshAI GACnn'nnGTC
Cuts at: 1613 3566 1613
Size: 1953 6385
PsiI TTA'TAA
Cuts at: 5908 5908
Size: 8338
PspOMI G'GGCC_C
Cuts at: 3595 3720 4135 3595
Size: 125 415 7798
Fragments arranged by size:
7798 415 125
PstI C_TGCA'G
Cuts at: 830 3870 5690 830
Size: 3040 1820 3478
Fragments arranged by size:
3478 3040 1820
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8338
PvuII CAG'CTG
Cuts at: 3143 3547 3581 3143
Size: 404 34 7900
Fragments arranged by size:
7900 404 34
SacI G_AGCT'C
Cuts at: 4028 4415 4028
Size: 387 7951
SalI G'TCGA_C
Cuts at: 1936 5680 1936
Size: 3744 4594
SanDI GG'GwC_CC
Cuts at: 5352 5352
Size: 8338
SapI GCTCTTCn'nnn_
Cuts at: 2536 2536
Size: 8338
SbfI CC_TGCA'GG
Cuts at: 3870 5690 3870
Size: 1820 6518
ScaI AGT'ACT
Cuts at: 2221 6586 8298 2221
Size: 4365 1712 2261
Fragments arranged by size:
4365 2261 1712
SexAI A'CCwGG_T
Cuts at: 5580 5580
Size: 8338
SfcI C'TryA_G
Cuts at: 826 1045 3866 5070 5686 6821 7502 7693 826
Size: 219 2821 1204 616 1135 681 191 1471
Fragments arranged by size:
2821 1471 1204 1135 681 616 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8338
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8338
SmaI CCC'GGG
Cuts at: 1872 3719 3845 3959 4336 5665 1872
Size: 1847 126 114 377 1329 4545
Fragments arranged by size:
4545 1847 1329 377 126 114
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2781 4423 4649 4869 6442 7343
Size: 197 926 838 1642 226 220 1573 901
Cuts at: 7343 7605 820
Size: 262 1553
Fragments arranged by size:
1642 1573 1553 926 901 838 262 226
220 197
SnaBI TAC'GTA
Cuts at: 493 6129 493
Size: 5636 2702
SpeI A'CTAG_T
Cuts at: 152 2330 4166 152
Size: 2178 1836 4324
Fragments arranged by size:
4324 2178 1836
SphI G_CATG'C
Cuts at: 1132 5161 5696 1132
Size: 4029 535 3774
Fragments arranged by size:
4029 3774 535
SrfI GCCC'GGGC
Cuts at: 3845 3845
Size: 8338
SspI AAT'ATT
Cuts at: 5 52 5142 6262 5
Size: 47 5090 1120 2081
Fragments arranged by size:
5090 2081 1120 47
StuI AGG'CCT
Cuts at: 1517 3836 1517
Size: 2319 6019
StyI C'CwwG_G
Cuts at: 513 1065 2004 2494 2742 2892 3933 5553 5777
Size: 552 939 490 248 150 1041 1620 224
Cuts at: 5777 513
Size: 3074
Fragments arranged by size:
3074 1620 1041 939 552 490 248 224
150
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2553 3642 6365 6382 6535 6720 7165
Size: 1255 973 1089 2723 17 153 185 445
Cuts at: 7165 325
Size: 1498
Fragments arranged by size:
2723 1498 1255 1089 973 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2025 2219 3137
Size: 274 80 33 51 1443 48 194 918
Cuts at: 3137 6584 8296 96
Size: 3447 1712 138
Fragments arranged by size:
3447 1712 1443 918 274 194 138 80
51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2330 2362 4010 4042 8298 8330 2330
Size: 32 1648 32 4256 32 2338
Fragments arranged by size:
4256 2338 1648 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8338
XbaI T'CTAG_A
Cuts at: 5674 5674
Size: 8338
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2492 5179 5363 2492
Size: 2687 184 5467
Fragments arranged by size:
5467 2687 184
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8338
XmaI C'CCGG_G
Cuts at: 1870 3717 3843 3957 4334 5663 1870
Size: 1847 126 114 377 1329 4545
Fragments arranged by size:
4545 1847 1329 377 126 114
XmnI GAAnn'nnTTC
Cuts at: 2115 2210 3078 6467 2115
Size: 95 868 3389 3986
Fragments arranged by size:
3986 3389 868 95
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6146 276
Size: 53 83 186 936 4612 2468
Fragments arranged by size:
4612 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PspXI PsrI RsrII SacII SfiI SfoI SwaI