Restriction map for
pFN21AB9477
(Circular) (Six-base) MAPSORT of: pFN21AB9477.seq Check: 5916 from: 1 to: 5704
pFN21AB9477 5704 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2967 3514 278
Size: 53 83 186 936 1431 547 2468
Fragments arranged by size:
2468 1431 936 547 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3047 1937
Size: 1110 4594
Acc65I G'GTAC_C
Cuts at: 3025 3025
Size: 5704
AclI AA'CG_TT
Cuts at: 1563 3831 4204 1563
Size: 2268 373 3063
Fragments arranged by size:
3063 2268 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2651 3767 5151 5419 1632
Size: 1019 1116 1384 268 1917
Fragments arranged by size:
1917 1384 1116 1019 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5507
AflIII A'CryG_T
Cuts at: 1203 4603 5580 1203
Size: 3400 977 1327
Fragments arranged by size:
3400 1327 977
AgeI A'CCGG_T
Cuts at: 4535 4535
Size: 5704
AhdI GACnn_n'nnGTC
Cuts at: 4433 4433
Size: 5704
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 4006
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5704
AlwNI CAG_nnn'CTG
Cuts at: 2560 3447 5019 2560
Size: 887 1572 3245
Fragments arranged by size:
3245 1572 887
ApaI G_GGCC'C
Cuts at: 2132 2753 2132
Size: 621 5083
ApaLI G'TGCA_C
Cuts at: 3761 4917 3761
Size: 1156 4548
ApoI r'AATT_y
Cuts at: 1493 3013 3239 3391 5500 1493
Size: 1520 226 152 2109 1697
Fragments arranged by size:
2109 1697 1520 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4258 160
Size: 872 3226 1606
Fragments arranged by size:
3226 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
AvaI C'yCGr_G
Cuts at: 1870 1943 2432 2610 2745 3029 1870
Size: 73 489 178 135 284 4545
Fragments arranged by size:
4545 489 284 178 135 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2818 2851 2818
Size: 33 5671
BamHI G'GATC_C
Cuts at: 3034 3401 3034
Size: 367 5337
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2972 3025 4480 618
Size: 325 217 627 248 937 53 1455 1842
Fragments arranged by size:
1842 1455 937 627 325 248 217 53
BanII G_rGCy'C
Cuts at: 1391 2132 2439 2753 3023 1391
Size: 741 307 314 270 4072
Fragments arranged by size:
4072 741 314 307 270
BbeI G_GCGC'C
Cuts at: 2039 2976 2039
Size: 937 4767
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4733
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2482 2516 2638 2672 3895
Size: 34 50 34 461 34 122 34 1223
Cuts at: 3895 3929 1903
Size: 34 3678
Fragments arranged by size:
3678 1223 461 122 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3596 4806 3596
Size: 1210 4494
BclI T'GATC_A
Cuts at: 1548 2664 1548
Size: 1116 4588
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2565 2694 4315
Size: 107 122 71 631 605 893 129 1621
Cuts at: 4315 136
Size: 1525
Fragments arranged by size:
1621 1525 893 631 605 129 122 107
71
BglII A'GATC_T
Cuts at: 5699 5699
Size: 5704
BlpI GC'TnA_GC
Cuts at: 2064 3121 2064
Size: 1057 4647
Bme1580I G_kGCm'C
Cuts at: 1790 2132 2717 2753 3765 4921 1790
Size: 342 585 36 1012 1156 2573
Fragments arranged by size:
2573 1156 1012 585 342 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5704
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2027 2720 3435
Size: 416 53 83 399 24 610 693 715
Cuts at: 3435 4393 442
Size: 958 1753
Fragments arranged by size:
1753 958 715 693 610 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5704
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2840 4364 1239
Size: 1142 459 1524 2579
Fragments arranged by size:
2579 1524 1142 459
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2582 3829 4694 4992 2582
Size: 1247 865 298 3294
Fragments arranged by size:
3294 1247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4367 882
Size: 617 2868 2219
Fragments arranged by size:
2868 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2383 3495 493
Size: 711 1179 1112 2702
Fragments arranged by size:
2702 1179 1112 711
BsaBI GATnn'nnATC
Cuts at: 5698 5698
Size: 5704
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2036 2964 2973 2985
Size: 53 83 186 936 503 928 9 12
Cuts at: 2985 3511 3893 4528 275
Size: 526 382 635 1451
Fragments arranged by size:
1451 936 928 635 526 503 382 186
83 53 12 9
BsaWI w'CCGG_w
Cuts at: 4137 4535 4809 4956 5367 4137
Size: 398 274 147 411 4474
Fragments arranged by size:
4474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5479
Fragments arranged by size:
5479 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 4818
BseYI C'CCAG_C
Cuts at: 2011 2083 2158 2190 2563 2872 2926 4907 5403
Size: 72 75 32 373 309 54 1981 496
Cuts at: 5403 2011
Size: 2312
Fragments arranged by size:
2312 1981 496 373 309 75 72 54
32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2287 1163
Size: 573 551 4580
Fragments arranged by size:
4580 573 551
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2998 3154 3915 4943 1540
Size: 275 186 997 156 761 1028 2301
Fragments arranged by size:
2301 1028 997 761 275 186 156
BsiHKAI G_wGCw'C
Cuts at: 3765 3850 4921 3765
Size: 85 1071 4548
Fragments arranged by size:
4548 1071 85
BsmI GAATG_Cn'
Cuts at: 3215 3308 3215
Size: 93 5611
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2439 2717 2753 3023 3765 3850
Size: 399 342 307 278 36 270 742 85
Cuts at: 3850 4921 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 342 307 278 270
85 36
BspEI T'CCGG_A
Cuts at: 5367 5367
Size: 5704
BspHI T'CATG_A
Cuts at: 3593 5323 3593
Size: 1730 3974
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2823 3059 844
Size: 1979 236 3489
Fragments arranged by size:
3489 1979 236
BsrBI CCG'CTC
Cuts at: 1394 1823 3591 1394
Size: 429 1768 3507
Fragments arranged by size:
3507 1768 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4199 4373 65
Size: 1183 2951 174 1396
Fragments arranged by size:
2951 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2571 4348 4535 1700
Size: 110 761 1777 187 2869
Fragments arranged by size:
2869 1777 761 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5704
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5704
BssSI C'ACGA_G
Cuts at: 2177 3764 4776 2177
Size: 1587 1012 3105
Fragments arranged by size:
3105 1587 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2560 794
Size: 1766 3938
BstBI TT'CG_AA
Cuts at: 4508 4508
Size: 5704
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5704
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2866 2011
Size: 855 4849
BstYI r'GATC_y
Cuts at: 1972 2767 3034 3401 3786 3803 5255 5341 5353
Size: 795 267 367 385 17 1452 86 12
Cuts at: 5353 5699 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 795 385 367 346 267 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2074 2200 2757 2862 513
Size: 552 1009 126 557 105 3355
Fragments arranged by size:
3355 1009 557 552 126 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4965
Fragments arranged by size:
4965 683 56
BtsI GCAGTG_nn'
Cuts at: 3220 4014 4034 3220
Size: 794 20 4890
Fragments arranged by size:
4890 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5704
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5669
DraI TTT'AAA
Cuts at: 3008 3008
Size: 5704
DraIII CAC_nnn'GTG
Cuts at: 2323 2323
Size: 5704
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2954 2990 4711 809
Size: 507 1638 36 1721 1802
Fragments arranged by size:
1802 1721 1638 507 36
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2215 2686 2719
Size: 54 1425 50 275 18 385 471 33
Cuts at: 2719 2856 2904 2995 3151 4040 5669 8
Size: 137 48 91 156 889 1629 43
Fragments arranged by size:
1629 1425 889 471 385 275 156 137
91 54 50 48 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 2995 3151 1537
Size: 275 1183 156 4090
Fragments arranged by size:
4090 1183 275 156
EarI CTCTTCn'nnn_
Cuts at: 1353 2781 3634 1353
Size: 1428 853 3423
Fragments arranged by size:
3423 1428 853
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2041 2127 2264 4291 4665 4811
Size: 1064 138 452 86 137 2027 374 146
Cuts at: 4811 387
Size: 1280
Fragments arranged by size:
2027 1280 1064 452 374 146 138 137
86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2651 2840 3767 4364 5151 5419
Size: 393 749 270 189 927 597 787 268
Cuts at: 5419 1239
Size: 1524
Fragments arranged by size:
1524 927 787 749 597 393 270 268
189
EcoO109I rG'GnC_Cy
Cuts at: 2102 2128 2129 2727 2749 2750 2911 2102
Size: 26 1 598 22 1 161 4895
Fragments arranged by size:
4895 598 161 26 22 1 1
EcoRI G'AATT_C
Cuts at: 3013 3391 3013
Size: 378 5326
FspI TGC'GCA
Cuts at: 4210 4210
Size: 5704
HaeII r_GCGC'y
Cuts at: 1678 2039 2976 4851 1678
Size: 361 937 1875 2531
Fragments arranged by size:
2531 1875 937 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2759 2791 4345 4377 4419
Size: 32 135 32 1187 32 1554 32 42
Cuts at: 4419 4451 1373
Size: 32 2626
Fragments arranged by size:
2626 1554 1187 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3048 3294 669
Size: 988 281 1110 246 3079
Fragments arranged by size:
3079 1110 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5704
HpaI GTT'AAC
Cuts at: 3294 3294
Size: 5704
KasI G'GCGC_C
Cuts at: 2035 2972 2035
Size: 937 4767
KpnI G_GTAC'C
Cuts at: 3029 3029
Size: 5704
MfeI C'AATT_G
Cuts at: 3303 3303
Size: 5704
MluI A'CGCG_T
Cuts at: 5580 5580
Size: 5704
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2400 4818 5002 1305
Size: 1095 2418 184 2007
Fragments arranged by size:
2418 2007 1095 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3882
Fragments arranged by size:
3882 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2522 2864 3662 4021 4180 518
Size: 648 1356 342 798 359 159 2042
Fragments arranged by size:
2042 1356 798 648 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2011 2194 2681 3120 3797 4945 5190 1606
Size: 405 183 487 439 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 487 439 405 245 183
NaeI GCC'GGC
Cuts at: 2573 2573
Size: 5704
NarI GG'CG_CC
Cuts at: 2036 2973 2036
Size: 937 4767
NcoI C'CATG_G
Cuts at: 513 1065 2200 2862 513
Size: 552 1135 662 3355
Fragments arranged by size:
3355 1135 662 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5704
NgoMIV G'CCGG_C
Cuts at: 2571 2571
Size: 5704
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5704
NotI GC'GGCC_GC
Cuts at: 3151 3151
Size: 5704
NspI r_CATG'y
Cuts at: 1132 1229 3062 4607 1132
Size: 97 1833 1545 2229
Fragments arranged by size:
2229 1833 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5704
PciI A'CATG_T
Cuts at: 4603 4603
Size: 5704
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2075 2323 1108
Size: 967 248 4489
Fragments arranged by size:
4489 967 248
PmeI GTTT'AAAC
Cuts at: 3008 3008
Size: 5704
PmlI CAC'GTG
Cuts at: 2383 2383
Size: 5704
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2314 3773 3805 5286 5318 2282
Size: 32 1459 32 1481 32 2668
Fragments arranged by size:
2668 1481 1459 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2727 2727
Size: 5704
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5704
PsiI TTA'TAA
Cuts at: 3274 3274
Size: 5704
PspOMI G'GGCC_C
Cuts at: 2128 2749 2128
Size: 621 5083
PstI C_TGCA'G
Cuts at: 830 3056 830
Size: 2226 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5704
PvuII CAG'CTG
Cuts at: 2194 2194
Size: 5704
SalI G'TCGA_C
Cuts at: 1936 3046 1936
Size: 1110 4594
SanDI GG'GwC_CC
Cuts at: 2727 2727
Size: 5704
SbfI CC_TGCA'GG
Cuts at: 3056 3056
Size: 5704
ScaI AGT'ACT
Cuts at: 3952 5664 3952
Size: 1712 3992
SfcI C'TryA_G
Cuts at: 826 1045 3052 4187 4868 5059 826
Size: 219 2007 1135 681 191 1471
Fragments arranged by size:
2007 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2037 2974 2037
Size: 937 4767
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5704
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5704
SmaI CCC'GGG
Cuts at: 1872 2747 3031 1872
Size: 875 284 4545
Fragments arranged by size:
4545 875 284
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2597 3808 4709 4971 820
Size: 197 926 654 1211 901 262 1553
Fragments arranged by size:
1553 1211 926 901 654 262 197
SnaBI TAC'GTA
Cuts at: 493 3495 493
Size: 3002 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5704
SphI G_CATG'C
Cuts at: 1132 3062 1132
Size: 1930 3774
SspI AAT'ATT
Cuts at: 5 52 3628 5
Size: 47 3576 2081
Fragments arranged by size:
3576 2081 47
StuI AGG'CCT
Cuts at: 1517 2270 2654 1517
Size: 753 384 4567
Fragments arranged by size:
4567 753 384
StyI C'CwwG_G
Cuts at: 513 1065 2200 2862 2988 3143 513
Size: 552 1135 662 126 155 3074
Fragments arranged by size:
3074 1135 662 552 155 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2330 2918 3731 3748 3901 4086
Size: 1255 624 126 588 813 17 153 185
Cuts at: 4086 4531 325
Size: 445 1498
Fragments arranged by size:
1498 1255 813 624 588 445 185 153
126 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3950 5662 96
Size: 274 80 33 51 1443 1973 1712 138
Fragments arranged by size:
1973 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5664 5696 5664
Size: 32 5672
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5704
XbaI T'CTAG_A
Cuts at: 3040 3040
Size: 5704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2198 2803 2869 2198
Size: 605 66 5033
Fragments arranged by size:
5033 605 66
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5704
XmaI C'CCGG_G
Cuts at: 1870 2745 3029 1870
Size: 875 284 4545
Fragments arranged by size:
4545 875 284
XmnI GAAnn'nnTTC
Cuts at: 3833 3833
Size: 5704
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2965 3512 276
Size: 53 83 186 936 1431 547 2468
Fragments arranged by size:
2468 1431 936 547 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SacII SapI SexAI SfiI SrfI
SwaI