Restriction map for
pFN21AB9473
(Circular) (Six-base) MAPSORT of: pFN21AB9473.seq Check: 6011 from: 1 to: 7201
pFN21AB9473 7201 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5011 278
Size: 53 83 186 936 3475 2468
Fragments arranged by size:
3475 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2157 4544 1937
Size: 220 2387 4594
Fragments arranged by size:
4594 2387 220
Acc65I G'GTAC_C
Cuts at: 4522 4522
Size: 7201
AclI AA'CG_TT
Cuts at: 1563 5328 5701 1563
Size: 3765 373 3063
Fragments arranged by size:
3765 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2522 2823 3785 5264 6648 6916 1632
Size: 890 301 962 1479 1384 268 1917
Fragments arranged by size:
1917 1479 1384 962 890 301 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7004
AflIII A'CryG_T
Cuts at: 1203 6100 7077 1203
Size: 4897 977 1327
Fragments arranged by size:
4897 1327 977
AgeI A'CCGG_T
Cuts at: 6032 6032
Size: 7201
AhdI GACnn_n'nnGTC
Cuts at: 5930 5930
Size: 7201
AleI CACnn'nnGTG
Cuts at: 1166 4271 1166
Size: 3105 4096
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7201
AlwNI CAG_nnn'CTG
Cuts at: 2434 4320 4944 6516 2434
Size: 1886 624 1572 3119
Fragments arranged by size:
3119 1886 1572 624
ApaLI G'TGCA_C
Cuts at: 5258 6414 5258
Size: 1156 6045
ApoI r'AATT_y
Cuts at: 1493 2699 3330 3993 4510 4736 4888 6997 1493
Size: 1206 631 663 517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1206 663 631 517 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5755 160
Size: 872 4723 1606
Fragments arranged by size:
4723 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 4344 4526 1870
Size: 73 126 2275 182 4545
Fragments arranged by size:
4545 2275 182 126 73
BamHI G'GATC_C
Cuts at: 4531 4898 4531
Size: 367 6834
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2073 2104 2138 2461 4522
Size: 325 217 627 286 31 34 323 2061
Cuts at: 4522 5977 618
Size: 1455 1842
Fragments arranged by size:
2061 1842 1455 627 325 323 286 217
34 31
BanII G_rGCy'C
Cuts at: 1391 3580 4520 1391
Size: 2189 940 4072
Fragments arranged by size:
4072 2189 940
BbeI G_GCGC'C
Cuts at: 2142 2142
Size: 7201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2938 4489 928
Size: 971 1039 1551 3640
Fragments arranged by size:
3640 1551 1039 971
BbvCI CC'TCA_GC
Cuts at: 2177 3932 2177
Size: 1755 5446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5392 5426 1903
Size: 34 50 34 3371 34 3678
Fragments arranged by size:
3678 3371 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4043 5093 6303 4043
Size: 1050 1210 4941
Fragments arranged by size:
4941 1210 1050
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7201
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5812 136
Size: 107 122 71 631 605 4140 1525
Fragments arranged by size:
4140 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7196 7196
Size: 7201
BlpI GC'TnA_GC
Cuts at: 4618 4618
Size: 7201
Bme1580I G_kGCm'C
Cuts at: 1790 2076 2672 5262 6418 1790
Size: 286 596 2590 1156 2573
Fragments arranged by size:
2590 2573 1156 596 286
BmgBI CAC'GTC
Cuts at: 1318 2593 1318
Size: 1275 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4235 4932 5890
Size: 416 53 83 399 24 2818 697 958
Cuts at: 5890 442
Size: 1753
Fragments arranged by size:
2818 1753 958 697 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3929 1056
Size: 2873 4328
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3148 3148
Size: 7201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3118 5861 1239
Size: 1879 2743 2579
Fragments arranged by size:
2743 2579 1879
Bpu10I CC'TnA_GC
Cuts at: 2177 3932 2177
Size: 1755 5446
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5326 6191 6489 5326
Size: 865 298 6038
Fragments arranged by size:
6038 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3638 4476 5864 882
Size: 617 2139 838 1388 2219
Fragments arranged by size:
2219 2139 1388 838 617
BsaAI yAC'GTr
Cuts at: 493 1204 3517 4992 493
Size: 711 2313 1475 2702
Fragments arranged by size:
2702 2313 1475 711
BsaBI GATnn'nnATC
Cuts at: 2148 7195 2148
Size: 5047 2154
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2063 2139 3474 5008
Size: 53 83 186 936 530 76 1335 1534
Cuts at: 5008 5390 6025 275
Size: 382 635 1451
Fragments arranged by size:
1534 1451 1335 936 635 530 382 186
83 76 53
BsaWI w'CCGG_w
Cuts at: 2517 3258 3303 5634 6032 6306 6453 6864 2517
Size: 741 45 2331 398 274 147 411 2854
Fragments arranged by size:
2854 2331 741 411 398 274 147 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6976
Fragments arranged by size:
6976 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2371 3148 3221 1186
Size: 1185 777 73 5166
Fragments arranged by size:
5166 1185 777 73
BseYI C'CCAG_C
Cuts at: 4159 4320 6404 6900 4159
Size: 161 2084 496 4460
Fragments arranged by size:
4460 2084 496 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6628
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4338 4651 5412 6440 1540
Size: 275 186 2337 313 761 1028 2301
Fragments arranged by size:
2337 2301 1028 761 313 275 186
BsiHKAI G_wGCw'C
Cuts at: 2476 3463 3678 5262 5347 6418 2476
Size: 987 215 1584 85 1071 3259
Fragments arranged by size:
3259 1584 1071 987 215 85
BsmI GAATG_Cn'
Cuts at: 4365 4712 4805 4365
Size: 347 93 6761
Fragments arranged by size:
6761 347 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2408 2408
Size: 7201
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2476 2672 3463 3580 3678 4520
Size: 399 286 400 196 791 117 98 842
Cuts at: 4520 5262 5347 6418 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 842 791 742 400 399 286
196 117 98 85
BspEI T'CCGG_A
Cuts at: 2517 3258 6864 2517
Size: 741 3606 2854
Fragments arranged by size:
3606 2854 741
BspHI T'CATG_A
Cuts at: 4209 5090 6820 4209
Size: 881 1730 4590
Fragments arranged by size:
4590 1730 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2314 4556 844
Size: 1470 2242 3489
Fragments arranged by size:
3489 2242 1470
BsrBI CCG'CTC
Cuts at: 1394 1823 4249 5088 1394
Size: 429 2426 839 3507
Fragments arranged by size:
3507 2426 839 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3078 3354 3651 5696 5870 65
Size: 1183 1830 276 297 2045 174 1396
Fragments arranged by size:
2045 1830 1396 1183 297 276 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5845 6032 1700
Size: 110 4035 187 2869
Fragments arranged by size:
4035 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3738 96
Size: 3642 3559
BssHII G'CGCG_C
Cuts at: 1925 2037 1925
Size: 112 7089
BssSI C'ACGA_G
Cuts at: 5261 6273 5261
Size: 1012 6189
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7201
BstBI TT'CG_AA
Cuts at: 6005 6005
Size: 7201
BstEII G'GTnAC_C
Cuts at: 1184 3844 1184
Size: 2660 4541
BstXI CCAn_nnnn'nTGG
Cuts at: 2593 2593
Size: 7201
BstYI r'GATC_y
Cuts at: 1972 2822 4488 4531 4898 5283 5300 6752 6838
Size: 850 1666 43 367 385 17 1452 86
Cuts at: 6838 6850 7196 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1666 1452 850 385 367 346 86
43 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2457 3201 4011 513
Size: 552 1392 744 810 3703
Fragments arranged by size:
3703 1392 810 744 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6462
Fragments arranged by size:
6462 683 56
BtsI GCAGTG_nn'
Cuts at: 4046 4717 5511 5531 4046
Size: 671 794 20 5716
Fragments arranged by size:
5716 794 671 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7201
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7166
DraI TTT'AAA
Cuts at: 4505 4505
Size: 7201
DraIII CAC_nnn'GTG
Cuts at: 2695 2695
Size: 7201
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6208 809
Size: 507 4892 1802
Fragments arranged by size:
4892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4263 4335 4648
Size: 54 1425 50 275 18 2433 72 313
Cuts at: 4648 5537 7166 8
Size: 889 1629 43
Fragments arranged by size:
2433 1629 1425 889 313 275 72 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4335 4648 1537
Size: 275 2523 313 4090
Fragments arranged by size:
4090 2523 313 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2342 3539 3641 4256 5131 1353
Size: 989 1197 102 615 875 3423
Fragments arranged by size:
3423 1197 989 875 615 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5788 6162 6308 387
Size: 1064 138 4199 374 146 1280
Fragments arranged by size:
4199 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2522 2823 3118 3785 5264 5861 6648
Size: 393 890 301 295 667 1479 597 787
Cuts at: 6648 6916 1239
Size: 268 1524
Fragments arranged by size:
1524 1479 890 787 667 597 393 301
295 268
EcoO109I rG'GnC_Cy
Cuts at: 2420 4234 2420
Size: 1814 5387
EcoRI G'AATT_C
Cuts at: 4510 4888 4510
Size: 378 6823
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3613 3613
Size: 7201
FspI TGC'GCA
Cuts at: 5707 5707
Size: 7201
HaeII r_GCGC'y
Cuts at: 1678 2142 3807 6348 1678
Size: 464 1665 2541 2531
Fragments arranged by size:
2541 2531 1665 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2300 2332 2438 2470 2582
Size: 32 135 32 728 32 106 32 112
Cuts at: 2582 2614 2845 2877 4473 4505 5842 5874 5916
Size: 32 231 32 1596 32 1337 32 42
Cuts at: 5916 5948 1373
Size: 32 2626
Fragments arranged by size:
2626 1596 1337 728 231 135 112 106
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4099 4449 4545 4791 669
Size: 988 281 2161 350 96 246 3079
Fragments arranged by size:
3079 2161 988 350 281 246 96
HindIII A'AGCT_T
Cuts at: 748 3031 748
Size: 2283 4918
HpaI GTT'AAC
Cuts at: 4099 4449 4791 4099
Size: 350 342 6509
Fragments arranged by size:
6509 350 342
KasI G'GCGC_C
Cuts at: 2138 2138
Size: 7201
KpnI G_GTAC'C
Cuts at: 4526 4526
Size: 7201
MfeI C'AATT_G
Cuts at: 4800 4800
Size: 7201
MluI A'CGCG_T
Cuts at: 7077 7077
Size: 7201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3735 6315 6499 1305
Size: 2430 2580 184 2007
Fragments arranged by size:
2580 2430 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4265 10
Size: 54 1425 343 2433 2946
Fragments arranged by size:
2946 2433 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2612 3359 3740 4208 4271 5159 5518
Size: 648 1446 747 381 468 63 888 359
Cuts at: 5518 5677 518
Size: 159 2042
Fragments arranged by size:
2042 1446 888 747 648 468 381 359
159 63
MspA1I CmG'CkG
Cuts at: 1606 2163 3388 4271 4320 4617 5294 6442 6687
Size: 557 1225 883 49 297 677 1148 245
Cuts at: 6687 1606
Size: 2120
Fragments arranged by size:
2120 1225 1148 883 677 557 297 245
49
NarI GG'CG_CC
Cuts at: 2139 2139
Size: 7201
NcoI C'CATG_G
Cuts at: 513 1065 2457 4011 513
Size: 552 1392 1554 3703
Fragments arranged by size:
3703 1554 1392 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7201
NheI G'CTAG_C
Cuts at: 1052 3925 1052
Size: 2873 4328
NotI GC'GGCC_GC
Cuts at: 4648 4648
Size: 7201
NspI r_CATG'y
Cuts at: 1132 1229 3763 4159 4559 6104 1132
Size: 97 2534 396 400 1545 2229
Fragments arranged by size:
2534 2229 1545 400 396 97
PasI CC'CwG_GG
Cuts at: 1348 2467 4137 1348
Size: 1119 1670 4412
Fragments arranged by size:
4412 1670 1119
PciI A'CATG_T
Cuts at: 6100 6100
Size: 7201
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2695 3916 1108
Size: 1587 1221 4393
Fragments arranged by size:
4393 1587 1221
PfoI T'CCnGG_A
Cuts at: 2835 3139 3293 2835
Size: 304 154 6743
Fragments arranged by size:
6743 304 154
PmeI GTTT'AAAC
Cuts at: 4505 4505
Size: 7201
PmlI CAC'GTG
Cuts at: 3517 3517
Size: 7201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2180 2212 2243 2275 5270 5302 6783 6815 2180
Size: 32 31 32 2995 32 1481 32 2566
Fragments arranged by size:
2995 2566 1481 32 32 32 32 31
PpuMI rG'GwC_Cy
Cuts at: 2420 4234 2420
Size: 1814 5387
PshAI GACnn'nnGTC
Cuts at: 1613 2894 1613
Size: 1281 5920
PsiI TTA'TAA
Cuts at: 4771 4771
Size: 7201
PstI C_TGCA'G
Cuts at: 830 4553 830
Size: 3723 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7201
PvuII CAG'CTG
Cuts at: 3388 4320 3388
Size: 932 6269
SalI G'TCGA_C
Cuts at: 1936 4543 1936
Size: 2607 4594
SanDI GG'GwC_CC
Cuts at: 2420 2420
Size: 7201
SapI GCTCTTCn'nnn_
Cuts at: 2342 4256 2342
Size: 1914 5287
SbfI CC_TGCA'GG
Cuts at: 4553 4553
Size: 7201
ScaI AGT'ACT
Cuts at: 5449 7161 5449
Size: 1712 5489
SexAI A'CCwGG_T
Cuts at: 2223 2223
Size: 7201
SfcI C'TryA_G
Cuts at: 826 1045 2158 2747 2864 4549 5684 6365 6556
Size: 219 1113 589 117 1685 1135 681 191
Cuts at: 6556 826
Size: 1471
Fragments arranged by size:
1685 1471 1135 1113 681 589 219 191
117
SfoI GGC'GCC
Cuts at: 2140 2140
Size: 7201
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7201
SmaI CCC'GGG
Cuts at: 1872 2071 4346 4528 1872
Size: 199 2275 182 4545
Fragments arranged by size:
4545 2275 199 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5305 6206 6468 820
Size: 197 926 3362 901 262 1553
Fragments arranged by size:
3362 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4992 493
Size: 4499 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7201
SphI G_CATG'C
Cuts at: 1132 3763 4559 1132
Size: 2631 796 3774
Fragments arranged by size:
3774 2631 796
SrfI GCCC'GGGC
Cuts at: 2071 2071
Size: 7201
SspI AAT'ATT
Cuts at: 5 52 5125 5
Size: 47 5073 2081
Fragments arranged by size:
5073 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7201
StyI C'CwwG_G
Cuts at: 513 1065 2341 2457 3468 3588 3853 4011 4640
Size: 552 1276 116 1011 120 265 158 629
Cuts at: 4640 513
Size: 3074
Fragments arranged by size:
3074 1276 1011 629 552 265 158 120
116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2459 3801 3953 5228 5245 5398 5583
Size: 1255 879 1342 152 1275 17 153 185
Cuts at: 5583 6028 325
Size: 445 1498
Fragments arranged by size:
1498 1342 1275 1255 879 445 185 153
152 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2478 3738 5447
Size: 274 80 33 51 1443 501 1260 1709
Cuts at: 5447 7159 96
Size: 1712 138
Fragments arranged by size:
1712 1709 1443 1260 501 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2377 2409 3048 3080 7161 7193 2377
Size: 32 639 32 4081 32 2385
Fragments arranged by size:
4081 2385 639 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7201
XbaI T'CTAG_A
Cuts at: 4537 4537
Size: 7201
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2464 2464
Size: 7201
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7201
XmaI C'CCGG_G
Cuts at: 1870 2069 4344 4526 1870
Size: 199 2275 182 4545
Fragments arranged by size:
4545 2275 199 182
XmnI GAAnn'nnTTC
Cuts at: 2507 5330 2507
Size: 2823 4378
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5009 276
Size: 53 83 186 936 3475 2468
Fragments arranged by size:
3475 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI
RsrII SacI SacII SfiI SwaI