Restriction map for
pFN21AB9472
(Circular) (Six-base) MAPSORT of: pFN21AB9472.seq Check: 9975 from: 1 to: 6217
pFN21AB9472 6217 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2110 2257 2672 2110
Size: 147 415 5655
Fragments arranged by size:
5655 415 147
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2276 2648 2867 4280 5664 5932 1632
Size: 644 372 219 1413 1384 268 1917
Fragments arranged by size:
1917 1413 1384 644 372 268 219
AfeI AGC'GCT
Cuts at: 3322 3322
Size: 6217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 2295 2934 3475 5116 6093 1203
Size: 1092 639 541 1641 977 1327
Fragments arranged by size:
1641 1327 1092 977 639 541
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 2938 4946 2938
Size: 2008 4209
AleI CACnn'nnGTG
Cuts at: 1166 2774 1166
Size: 1608 4609
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2223 2254 2845 3401 3960 5532 2223
Size: 31 591 556 559 1572 2908
Fragments arranged by size:
2908 1572 591 559 556 31
ApaI G_GGCC'C
Cuts at: 2391 3389 2391
Size: 998 5219
ApaLI G'TGCA_C
Cuts at: 3220 4274 5430 3220
Size: 1054 1156 4007
Fragments arranged by size:
4007 1156 1054
ApoI r'AATT_y
Cuts at: 1493 3526 3752 3904 6013 1493
Size: 2033 226 152 2109 1697
Fragments arranged by size:
2109 2033 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 2079 3542 1870
Size: 73 136 1463 4545
Fragments arranged by size:
4545 1463 136 73
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2472 2532 2687 2864 3023
Size: 325 217 627 685 60 155 177 159
Cuts at: 3023 3179 3252 3538 4993 618
Size: 156 73 286 1455 1842
Fragments arranged by size:
1842 1455 685 627 325 286 217 177
159 156 155 73 60
BanII G_rGCy'C
Cuts at: 1391 2018 2391 2601 3389 3536 1391
Size: 627 373 210 788 147 4072
Fragments arranged by size:
4072 788 627 373 210 147
BbeI G_GCGC'C
Cuts at: 2476 2691 2868 3027 2476
Size: 215 177 159 5666
Fragments arranged by size:
5666 215 177 159
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3160 928
Size: 971 1261 3985
Fragments arranged by size:
3985 1261 971
BbvCI CC'TCA_GC
Cuts at: 3317 3317
Size: 6217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4408 4442 1903
Size: 34 2471 34 3678
Fragments arranged by size:
3678 2471 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2251 4828 136
Size: 107 122 71 631 605 579 2577 1525
Fragments arranged by size:
2577 1525 631 605 579 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2595 3634 2595
Size: 1039 5178
Bme1580I G_kGCm'C
Cuts at: 1790 2159 2391 2440 2535 2989 3182 3224 3255
Size: 369 232 49 95 454 193 42 31
Cuts at: 3255 3389 4278 5434 1790
Size: 134 889 1156 2573
Fragments arranged by size:
2573 1156 889 454 369 232 193 134
95 49 42 31
BmgBI CAC'GTC
Cuts at: 1318 2796 2824 2935 1318
Size: 1478 28 111 4600
Fragments arranged by size:
4600 1478 111 28
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2153 3389 3392
Size: 416 53 83 399 24 736 1236 3
Cuts at: 3392 3948 4906 442
Size: 556 958 1753
Fragments arranged by size:
1753 1236 958 736 556 416 399 83
53 24 3
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2245 4877 1239
Size: 1006 2632 2579
Fragments arranged by size:
2632 2579 1006
Bpu10I CC'TnA_GC
Cuts at: 2327 3317 2327
Size: 990 5227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2498 4342 5207 5505 2498
Size: 1844 865 298 3210
Fragments arranged by size:
3210 1844 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3363 4880 882
Size: 617 1864 1517 2219
Fragments arranged by size:
2219 1864 1517 617
BsaAI yAC'GTr
Cuts at: 493 1204 2296 2553 3478 4008 493
Size: 711 1092 257 925 530 2702
Fragments arranged by size:
2702 1092 925 711 530 257
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2473 2688 2865
Size: 53 83 186 936 476 464 215 177
Cuts at: 2865 3024 4024 4406 5041 275
Size: 159 1000 382 635 1451
Fragments arranged by size:
1451 1000 936 635 476 464 382 215
186 177 159 83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3341 3371 1178
Size: 30 165 30 1938 30 4024
Fragments arranged by size:
4024 1938 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2389 3097 3362 1186
Size: 1203 708 265 4041
Fragments arranged by size:
4041 1203 708 265
BseYI C'CCAG_C
Cuts at: 2050 2131 3154 3372 5420 5916 2050
Size: 81 1023 218 2048 496 2351
Fragments arranged by size:
2351 2048 1023 496 218 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5644
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2070 2444 2642 3280 3667 4428
Size: 275 186 69 374 198 638 387 761
Cuts at: 4428 5456 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 638 387 374 275 198
186 69
BsiHKAI G_wGCw'C
Cuts at: 2748 3224 4278 4363 5434 2748
Size: 476 1054 85 1071 3531
Fragments arranged by size:
3531 1071 1054 476 85
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2018 2159 2391 2440 2535 2601 2748
Size: 399 228 141 232 49 95 66 147
Cuts at: 2748 2989 3182 3224 3255 3389 3536 4278 4363
Size: 241 193 42 31 134 147 742 85
Cuts at: 4363 5434 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 241 232 228 193
147 147 141 134 95 85 66 49
42 31
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2110 2257 2643 2672 3572 844
Size: 1266 147 386 29 900 3489
Fragments arranged by size:
3489 1266 900 386 147 29
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 4104 1394
Size: 429 452 1829 3507
Fragments arranged by size:
3507 1829 452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2065 2285 2830 3158 4861 5048 1700
Size: 110 255 220 545 328 1703 187 2869
Fragments arranged by size:
2869 1703 545 328 255 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 3009 96
Size: 2913 3304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2896 3034 2896
Size: 138 6079
BstYI r'GATC_y
Cuts at: 1972 3547 3914 4299 4316 5768 5854 5866 6212
Size: 1575 367 385 17 1452 86 12 346
Cuts at: 6212 1972
Size: 1977
Fragments arranged by size:
1977 1575 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2114 2493 2925 3033 3131 3138 513
Size: 552 1049 379 432 108 98 7 3592
Fragments arranged by size:
3592 1049 552 432 379 108 98 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2159 2438 3733 4527 4547 2159
Size: 279 1295 794 20 3829
Fragments arranged by size:
3829 1295 794 279 20
ClaI AT'CG_AT
Cuts at: 1554 2892 1554
Size: 1338 4879
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DraIII CAC_nnn'GTG
Cuts at: 2764 2764
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2108 5224 809
Size: 507 792 3116 1802
Fragments arranged by size:
3116 1802 792 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2203 2523
Size: 54 1425 50 275 18 237 136 320
Cuts at: 2523 2639 2902 3117 3309 3664 4553 6182 8
Size: 116 263 215 192 355 889 1629 43
Fragments arranged by size:
1629 1425 889 355 320 275 263 237
215 192 136 116 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2067 2639 3664 1537
Size: 275 255 572 1025 4090
Fragments arranged by size:
4090 1025 572 275 255
EarI CTCTTCn'nnn_
Cuts at: 1353 4147 1353
Size: 2794 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2114 4804 5178 5324 387
Size: 1064 138 525 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 525 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2245 2276 2648 2867 4280 4877 5664
Size: 393 613 31 372 219 1413 597 787
Cuts at: 5664 5932 1239
Size: 268 1524
Fragments arranged by size:
1524 1413 787 613 597 393 372 268
219 31
EcoO109I rG'GnC_Cy
Cuts at: 2055 2084 2170 2387 2416 3295 2055
Size: 29 86 217 29 879 4977
Fragments arranged by size:
4977 879 217 86 29 29
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FspI TGC'GCA
Cuts at: 2816 4723 2816
Size: 1907 4310
HaeII r_GCGC'y
Cuts at: 1678 2476 2691 2868 3027 3057 3165 3324 5364
Size: 798 215 177 159 30 108 159 2040
Cuts at: 5364 1678
Size: 2531
Fragments arranged by size:
2531 2040 798 215 177 159 159 108
30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2924 2956 3287
Size: 32 135 32 812 32 508 32 331
Cuts at: 3287 3319 3341 3373 4858 4890 4932 4964 1373
Size: 32 22 32 1485 32 42 32 2626
Fragments arranged by size:
2626 1485 812 508 331 135 42 32
32 32 32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 2335 3561 3807 669
Size: 988 281 397 1226 246 3079
Fragments arranged by size:
3079 1226 988 397 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2472 2687 2864 3023 2472
Size: 215 177 159 5666
Fragments arranged by size:
5666 215 177 159
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5331 5515 1305
Size: 4026 184 2007
Fragments arranged by size:
4026 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2525 10
Size: 54 1425 343 693 3702
Fragments arranged by size:
3702 1425 693 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 2774 2894 4175 4534 4693 518
Size: 648 1389 219 120 1281 359 159 2042
Fragments arranged by size:
2042 1389 1281 648 359 219 159 120
MspA1I CmG'CkG
Cuts at: 1606 2044 2182 2220 2529 2594 2652 2725 3285
Size: 438 138 38 309 65 58 73 560
Cuts at: 3285 3633 4310 5458 5703 1606
Size: 348 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 560 438 348 309 245
138 73 65 58 38
NaeI GCC'GGC
Cuts at: 2287 3160 2287
Size: 873 5344
NarI GG'CG_CC
Cuts at: 2473 2688 2865 3024 2473
Size: 215 177 159 5666
Fragments arranged by size:
5666 215 177 159
NcoI C'CATG_G
Cuts at: 513 1065 3033 513
Size: 552 1968 3697
Fragments arranged by size:
3697 1968 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2285 3158 2285
Size: 873 5344
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3010 3575 5120 1132
Size: 97 1781 565 1545 2229
Fragments arranged by size:
2229 1781 1545 565 97
PasI CC'CwG_GG
Cuts at: 1348 2369 1348
Size: 1021 5196
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3401 1108
Size: 2293 3924
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PmlI CAC'GTG
Cuts at: 2553 3478 2553
Size: 925 5292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2416 3295 2170
Size: 246 879 5092
Fragments arranged by size:
5092 879 246
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 2387 3385 2387
Size: 998 5219
PstI C_TGCA'G
Cuts at: 830 2154 3569 830
Size: 1324 1415 3478
Fragments arranged by size:
3478 1415 1324
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SanDI GG'GwC_CC
Cuts at: 2170 2170
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 4465 6177 4465
Size: 1712 4505
SfcI C'TryA_G
Cuts at: 826 1045 2150 3040 3565 4700 5381 5572 826
Size: 219 1105 890 525 1135 681 191 1471
Fragments arranged by size:
1471 1135 1105 890 681 525 219 191
SfoI GGC'GCC
Cuts at: 2474 2689 2866 3025 2474
Size: 215 177 159 5666
Fragments arranged by size:
5666 215 177 159
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2513 4321 5222 5484 820
Size: 197 926 570 1808 901 262 1553
Fragments arranged by size:
1808 1553 926 901 570 262 197
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2573 1517
Size: 1056 5161
StyI C'CwwG_G
Cuts at: 513 1065 2196 3019 3033 3656 513
Size: 552 1131 823 14 623 3074
Fragments arranged by size:
3074 1131 823 623 552 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4244 4261 4414 4599 5044 325
Size: 1255 2664 17 153 185 445 1498
Fragments arranged by size:
2664 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3009 4463 6175
Size: 274 80 33 51 1443 1032 1454 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
1712 1454 1443 1032 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6177 6209 6177
Size: 32 6185
Tth111I GACn'n_nGTC
Cuts at: 1316 2794 1316
Size: 1478 4739
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 2893 2707
Size: 186 6031
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII
SacI SacII SapI SexAI SfiI SrfI SwaI