Restriction map for
pFN21AB9467
(Circular) (Six-base) MAPSORT of: pFN21AB9467.seq Check: 2052 from: 1 to: 8248
pFN21AB9467 8248 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2503 2503
Size: 8248
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6058 278
Size: 53 83 186 936 4522 2468
Fragments arranged by size:
4522 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3585 3762 4991 5591 1937
Size: 1648 177 1229 600 4594
Fragments arranged by size:
4594 1648 1229 600 177
Acc65I G'GTAC_C
Cuts at: 5569 5569
Size: 8248
AclI AA'CG_TT
Cuts at: 1563 5267 6375 6748 1563
Size: 3704 1108 373 3063
Fragments arranged by size:
3704 3063 1108 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4030 4330 4694 4949 6311 7695 7963 1632
Size: 2398 300 364 255 1362 1384 268 1917
Fragments arranged by size:
2398 1917 1384 1362 364 300 268 255
AfeI AGC'GCT
Cuts at: 2058 2058
Size: 8248
AflII C'TTAA_G
Cuts at: 820 1017 4741 820
Size: 197 3724 4327
Fragments arranged by size:
4327 3724 197
AflIII A'CryG_T
Cuts at: 1203 2276 2393 7147 8124 1203
Size: 1073 117 4754 977 1327
Fragments arranged by size:
4754 1327 1073 977 117
AgeI A'CCGG_T
Cuts at: 7079 7079
Size: 8248
AhdI GACnn_n'nnGTC
Cuts at: 6977 6977
Size: 8248
AleI CACnn'nnGTG
Cuts at: 1166 3453 3908 1166
Size: 2287 455 5506
Fragments arranged by size:
5506 2287 455
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4228 1184
Size: 3044 5204
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3921 3953 3921
Size: 32 8216
AlwNI CAG_nnn'CTG
Cuts at: 2807 5991 7563 2807
Size: 3184 1572 3492
Fragments arranged by size:
3492 3184 1572
ApaLI G'TGCA_C
Cuts at: 3540 4886 6305 7461 3540
Size: 1346 1419 1156 4327
Fragments arranged by size:
4327 1419 1346 1156
ApoI r'AATT_y
Cuts at: 1493 2290 2581 2594 2974 3084 3282 3748 4791
Size: 797 291 13 380 110 198 466 1043
Cuts at: 4791 4815 4843 4913 5125 5238 5557 5783 5935
Size: 24 28 70 212 113 319 226 152
Cuts at: 5935 8044 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1043 797 466 380 319 291
226 212 198 152 113 110 70 28
24 13
AseI AT'TA_AT
Cuts at: 160 1032 6802 160
Size: 872 5770 1606
Fragments arranged by size:
5770 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
AvaI C'yCGr_G
Cuts at: 1870 1943 3375 3641 4217 5573 1870
Size: 73 1432 266 576 1356 4545
Fragments arranged by size:
4545 1432 1356 576 266 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5000 5033 5000
Size: 33 8215
BamHI G'GATC_C
Cuts at: 3821 5578 5945 3821
Size: 1757 367 6124
Fragments arranged by size:
6124 1757 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3920 4173 5569 7024 618
Size: 325 217 627 2133 253 1396 1455 1842
Fragments arranged by size:
2133 1842 1455 1396 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 4245 5541 5567 1391
Size: 2854 1296 26 4072
Fragments arranged by size:
4072 2854 1296 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3242 4336 928
Size: 971 1343 1094 4840
Fragments arranged by size:
4840 1343 1094 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6439 6473 1903
Size: 34 4502 34 3678
Fragments arranged by size:
4502 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6140 7350 6140
Size: 1210 7038
BclI T'GATC_A
Cuts at: 1548 2893 1548
Size: 1345 6903
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3645 6859 136
Size: 107 122 71 631 605 1973 3214 1525
Fragments arranged by size:
3214 1973 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4829 8243 4829
Size: 3414 4834
BlpI GC'TnA_GC
Cuts at: 5665 5665
Size: 8248
Bme1580I G_kGCm'C
Cuts at: 1790 2413 3544 3788 4890 6309 7465 1790
Size: 623 1131 244 1102 1419 1156 2573
Fragments arranged by size:
2573 1419 1156 1131 1102 623 244
BmgBI CAC'GTC
Cuts at: 1318 3526 1318
Size: 2208 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5034 5979 6937
Size: 416 53 83 399 24 3617 945 958
Cuts at: 6937 442
Size: 1753
Fragments arranged by size:
3617 1753 958 945 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8248
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2405 2405
Size: 8248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2230 2400 3235 3363 3774 3833 4126 6908
Size: 991 170 835 128 411 59 293 2782
Cuts at: 6908 1239
Size: 2579
Fragments arranged by size:
2782 2579 991 835 411 293 170 128
59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4416 6373 7238 7536 4416
Size: 1957 865 298 5128
Fragments arranged by size:
5128 1957 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 5522 6911 882
Size: 617 609 3414 1389 2219
Fragments arranged by size:
3414 2219 1389 617 609
BsaAI yAC'GTr
Cuts at: 493 1204 6039 493
Size: 711 4835 2702
Fragments arranged by size:
4835 2702 711
BsaBI GATnn'nnATC
Cuts at: 4126 5073 8242 4126
Size: 947 3169 4132
Fragments arranged by size:
4132 3169 947
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4721 6055 6437 7072
Size: 53 83 186 936 3188 1334 382 635
Cuts at: 7072 275
Size: 1451
Fragments arranged by size:
3188 1451 1334 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2126 6681 7079 7353 7500 7911 2126
Size: 4555 398 274 147 411 2463
Fragments arranged by size:
4555 2463 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8023
Fragments arranged by size:
8023 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3129 3363 1186
Size: 1943 234 6071
Fragments arranged by size:
6071 1943 234
BseYI C'CCAG_C
Cuts at: 3065 3190 7451 7947 3065
Size: 125 4261 496 3366
Fragments arranged by size:
4261 3366 496 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2180 3177 3216 1163
Size: 573 444 997 39 6195
Fragments arranged by size:
6195 997 573 444 39
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5698 6459 7487 1540
Size: 275 186 3697 761 1028 2301
Fragments arranged by size:
3697 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3373 3531 3544 4224 4890 5541 6309 6394 7465
Size: 158 13 680 666 651 768 85 1071
Cuts at: 7465 3373
Size: 4156
Fragments arranged by size:
4156 1071 768 680 666 651 158 85
13
BsmI GAATG_Cn'
Cuts at: 2310 4024 5759 5852 2310
Size: 1714 1735 93 4706
Fragments arranged by size:
4706 1735 1714 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4728 4728
Size: 8248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2413 3373 3531 3544 3788 4224 4245
Size: 399 623 960 158 13 244 436 21
Cuts at: 4245 4890 5541 5567 6309 6394 7465 1391
Size: 645 651 26 742 85 1071 2174
Fragments arranged by size:
2174 1071 960 742 651 645 623 436
399 244 158 85 26 21 13
BspEI T'CCGG_A
Cuts at: 7911 7911
Size: 8248
BspHI T'CATG_A
Cuts at: 6137 7867 6137
Size: 1730 6518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2503 5189 5603 844
Size: 1659 2686 414 3489
Fragments arranged by size:
3489 2686 1659 414
BsrBI CCG'CTC
Cuts at: 1394 1823 6135 1394
Size: 429 4312 3507
Fragments arranged by size:
4312 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2423 6743 6917 65
Size: 1183 1175 4320 174 1396
Fragments arranged by size:
4320 1396 1183 1175 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3389 4573 6892 7079 1700
Size: 110 1579 1184 2319 187 2869
Fragments arranged by size:
2869 2319 1579 1184 187 110
BsrGI T'GTAC_A
Cuts at: 96 2599 3549 96
Size: 2503 950 4795
Fragments arranged by size:
4795 2503 950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8248
BssSI C'ACGA_G
Cuts at: 6308 7320 6308
Size: 1012 7236
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8248
BstBI TT'CG_AA
Cuts at: 7052 7052
Size: 8248
BstEII G'GTnAC_C
Cuts at: 1184 3455 1184
Size: 2271 5977
BstXI CCAn_nnnn'nTGG
Cuts at: 2371 2741 3857 2371
Size: 370 1116 6762
Fragments arranged by size:
6762 1116 370
BstYI r'GATC_y
Cuts at: 1972 3821 4829 5578 5945 6330 6347 7799 7885
Size: 1849 1008 749 367 385 17 1452 86
Cuts at: 7885 7897 8243 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1849 1452 1008 749 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3763 4992 3763
Size: 1229 7019
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6757
Fragments arranged by size:
6757 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4647 487
Size: 683 56 3421 4088
Fragments arranged by size:
4088 3421 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 2268 3204 5764 6558 6578 2163
Size: 105 936 2560 794 20 3833
Fragments arranged by size:
3833 2560 936 794 105 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4351 4386 529
Size: 35 3787 35 4391
Fragments arranged by size:
4391 3787 35 35
DraI TTT'AAA
Cuts at: 2076 3888 4156 4653 5552 2076
Size: 1812 268 497 899 4772
Fragments arranged by size:
4772 1812 899 497 268
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7255 809
Size: 507 5939 1802
Fragments arranged by size:
5939 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3247 3391 4086
Size: 54 1425 50 275 18 1417 144 695
Cuts at: 4086 5695 6584 8213 8
Size: 1609 889 1629 43
Fragments arranged by size:
1629 1609 1425 1417 889 695 275 144
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5695 1537
Size: 275 3883 4090
Fragments arranged by size:
4090 3883 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3172 3574 4864 6178 1353
Size: 1819 402 1290 1314 3423
Fragments arranged by size:
3423 1819 1314 1290 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6835 7209 7355 387
Size: 1064 138 5246 374 146 1280
Fragments arranged by size:
5246 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5539 5539
Size: 8248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2400 3235 3363 3774 3833 4030
Size: 393 598 170 835 128 411 59 197
Cuts at: 4030 4126 4330 4694 4949 6311 6908 7695 7963
Size: 96 204 364 255 1362 597 787 268
Cuts at: 7963 1239
Size: 1524
Fragments arranged by size:
1524 1362 835 787 598 597 411 393
364 268 255 204 197 170 128 96
59
EcoO109I rG'GnC_Cy
Cuts at: 2618 2746 3638 3864 2618
Size: 128 892 226 7002
Fragments arranged by size:
7002 892 226 128
EcoRI G'AATT_C
Cuts at: 3748 5557 5935 3748
Size: 1809 378 6061
Fragments arranged by size:
6061 1809 378
EcoRV GAT'ATC
Cuts at: 5071 5071
Size: 8248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5491 5491
Size: 8248
FspI TGC'GCA
Cuts at: 6754 6754
Size: 8248
HaeII r_GCGC'y
Cuts at: 1678 2060 7395 1678
Size: 382 5335 2531
Fragments arranged by size:
5335 2531 382
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2843 2875 5494 5526 6889
Size: 32 135 32 1271 32 2619 32 1363
Cuts at: 6889 6921 6963 6995 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2619 1363 1271 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2260 4012 4258 4473 4783 5592
Size: 988 281 322 1752 246 215 310 809
Cuts at: 5592 5838 669
Size: 246 3079
Fragments arranged by size:
3079 1752 988 809 322 310 281 246
246 215
HindIII A'AGCT_T
Cuts at: 748 3978 748
Size: 3230 5018
HpaI GTT'AAC
Cuts at: 4473 5838 4473
Size: 1365 6883
KpnI G_GTAC'C
Cuts at: 5573 5573
Size: 8248
MfeI C'AATT_G
Cuts at: 3675 5847 3675
Size: 2172 6076
MluI A'CGCG_T
Cuts at: 8124 8124
Size: 8248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3035 4438 7362 7546 1305
Size: 1730 1403 2924 184 2007
Fragments arranged by size:
2924 2007 1730 1403 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3249 4088 10
Size: 54 1425 343 1417 839 4170
Fragments arranged by size:
4170 1425 1417 839 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2416 3453 3698 3908 6206 6565 6724
Size: 648 1250 1037 245 210 2298 359 159
Cuts at: 6724 518
Size: 2042
Fragments arranged by size:
2298 2042 1250 1037 648 359 245 210
159
MspA1I CmG'CkG
Cuts at: 1606 2035 2041 4427 5294 5664 6341 7489 7734
Size: 429 6 2386 867 370 677 1148 245
Cuts at: 7734 1606
Size: 2120
Fragments arranged by size:
2386 2120 1148 867 677 429 370 245
6
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6757
Fragments arranged by size:
6757 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8248
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8248
NotI GC'GGCC_GC
Cuts at: 5695 5695
Size: 8248
NspI r_CATG'y
Cuts at: 1132 1229 2280 2397 4599 5606 7151 1132
Size: 97 1051 117 2202 1007 1545 2229
Fragments arranged by size:
2229 2202 1545 1051 1007 117 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8248
PciI A'CATG_T
Cuts at: 2276 2393 7147 2276
Size: 117 4754 3377
Fragments arranged by size:
4754 3377 117
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5241 1108
Size: 4133 4115
PmeI GTTT'AAAC
Cuts at: 5552 5552
Size: 8248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6317 6349 7830 7862 6317
Size: 32 1481 32 6703
Fragments arranged by size:
6703 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2746 3864 2746
Size: 1118 7130
PshAI GACnn'nnGTC
Cuts at: 1613 2460 3227 4284 1613
Size: 847 767 1057 5577
Fragments arranged by size:
5577 1057 847 767
PsiI TTA'TAA
Cuts at: 2171 5408 5818 2171
Size: 3237 410 4601
Fragments arranged by size:
4601 3237 410
PstI C_TGCA'G
Cuts at: 830 3291 4182 5156 5299 5600 830
Size: 2461 891 974 143 301 3478
Fragments arranged by size:
3478 2461 974 891 301 143
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8248
PvuII CAG'CTG
Cuts at: 4427 5294 4427
Size: 867 7381
SacI G_AGCT'C
Cuts at: 5541 5541
Size: 8248
SalI G'TCGA_C
Cuts at: 1936 5590 1936
Size: 3654 4594
SbfI CC_TGCA'GG
Cuts at: 5600 5600
Size: 8248
ScaI AGT'ACT
Cuts at: 3854 6496 8208 3854
Size: 2642 1712 3894
Fragments arranged by size:
3894 2642 1712
SfcI C'TryA_G
Cuts at: 826 1045 3287 3972 4178 5152 5167 5295 5596
Size: 219 2242 685 206 974 15 128 301
Cuts at: 5596 6731 7412 7603 826
Size: 1135 681 191 1471
Fragments arranged by size:
2242 1471 1135 974 685 681 301 219
206 191 128 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8248
SmaI CCC'GGG
Cuts at: 1872 5575 1872
Size: 3703 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4431 4741 6352 7253 7515 820
Size: 197 926 2488 310 1611 901 262 1553
Fragments arranged by size:
2488 1611 1553 926 901 310 262 197
SnaBI TAC'GTA
Cuts at: 493 6039 493
Size: 5546 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8248
SphI G_CATG'C
Cuts at: 1132 5606 1132
Size: 4474 3774
SspI AAT'ATT
Cuts at: 5 52 2121 6172 5
Size: 47 2069 4051 2081
Fragments arranged by size:
4051 2081 2069 47
StuI AGG'CCT
Cuts at: 1517 2607 1517
Size: 1090 7158
StyI C'CwwG_G
Cuts at: 513 1065 2004 2564 3867 4338 5687 513
Size: 552 939 560 1303 471 1349 3074
Fragments arranged by size:
3074 1349 1303 939 560 552 471
SwaI ATTT'AAAT
Cuts at: 3888 3888
Size: 8248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3182 6275 6292 6445 6630 7075 325
Size: 1255 1602 3093 17 153 185 445 1498
Fragments arranged by size:
3093 1602 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2599 3549 3852
Size: 274 80 33 51 1443 622 950 303
Cuts at: 3852 5278 6494 8206 96
Size: 1426 1216 1712 138
Fragments arranged by size:
1712 1443 1426 1216 950 622 303 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2383 2415 8208 8240 2383
Size: 32 5793 32 2391
Fragments arranged by size:
5793 2391 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8248
XbaI T'CTAG_A
Cuts at: 5584 5584
Size: 8248
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2184 4367 2184
Size: 2183 6065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8248
XmaI C'CCGG_G
Cuts at: 1870 5573 1870
Size: 3703 4545
XmnI GAAnn'nnTTC
Cuts at: 5161 6377 5161
Size: 1216 7032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6056 276
Size: 53 83 186 936 4522 2468
Fragments arranged by size:
4522 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI
Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI