Restriction map for
pFN21AB9465
(Circular) (Six-base) MAPSORT of: pFN21AB9465.seq Check: 8330 from: 1 to: 7816
pFN21AB9465 7816 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2023 3276 3892 4139 2023
Size: 1253 616 247 5700
Fragments arranged by size:
5700 1253 616 247
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5626 278
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5159 1937
Size: 3222 4594
Acc65I G'GTAC_C
Cuts at: 5137 5137
Size: 7816
AclI AA'CG_TT
Cuts at: 1563 5943 6316 1563
Size: 4380 373 3063
Fragments arranged by size:
4380 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2357 3239 3635 4359 5879 7263 7531 1632
Size: 725 882 396 724 1520 1384 268 1917
Fragments arranged by size:
1917 1520 1384 882 725 724 396 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7619
AflIII A'CryG_T
Cuts at: 1203 2625 6715 7692 1203
Size: 1422 4090 977 1327
Fragments arranged by size:
4090 1422 1327 977
AgeI A'CCGG_T
Cuts at: 6647 6647
Size: 7816
AhdI GACnn_n'nnGTC
Cuts at: 6545 6545
Size: 7816
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2288 2293 2677 3645 1184
Size: 1104 5 384 968 5355
Fragments arranged by size:
5355 1104 968 384 5
AlwNI CAG_nnn'CTG
Cuts at: 3268 3658 4039 5559 7131 3268
Size: 390 381 1520 1572 3953
Fragments arranged by size:
3953 1572 1520 390 381
ApaLI G'TGCA_C
Cuts at: 5873 7029 5873
Size: 1156 6660
ApoI r'AATT_y
Cuts at: 1493 3025 3296 4700 5125 5351 5503 7612 1493
Size: 1532 271 1404 425 226 152 2109 1697
Fragments arranged by size:
2109 1697 1532 1404 425 271 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2148 6370 160
Size: 872 1116 4222 1606
Fragments arranged by size:
4222 1606 1116 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
AvaI C'yCGr_G
Cuts at: 1870 1943 4428 4821 5141 1870
Size: 73 2485 393 320 4545
Fragments arranged by size:
4545 2485 393 320 73
BamHI G'GATC_C
Cuts at: 4470 5146 5513 4470
Size: 676 367 6773
Fragments arranged by size:
6773 676 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2035 2201 4121 5137 6592
Size: 325 217 627 248 166 1920 1016 1455
Cuts at: 6592 618
Size: 1842
Fragments arranged by size:
1920 1842 1455 1016 627 325 248 217
166
BanII G_rGCy'C
Cuts at: 1391 3324 4144 4305 5135 1391
Size: 1933 820 161 830 4072
Fragments arranged by size:
4072 1933 830 820 161
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3194 3590 4059 928
Size: 971 1295 396 469 4685
Fragments arranged by size:
4685 1295 971 469 396
BbvCI CC'TCA_GC
Cuts at: 2908 5049 2908
Size: 2141 5675
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4094 4128 4802 4836 6007 6041 1903
Size: 34 2157 34 674 34 1171 34 3678
Fragments arranged by size:
3678 2157 1171 674 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5708 6918 5708
Size: 1210 6606
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7816
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4211 4634 6427
Size: 107 122 71 631 605 2539 423 1793
Cuts at: 6427 136
Size: 1525
Fragments arranged by size:
2539 1793 1525 631 605 423 122 107
71
BglII A'GATC_T
Cuts at: 2815 3247 7811 2815
Size: 432 4564 2820
Fragments arranged by size:
4564 2820 432
BlpI GC'TnA_GC
Cuts at: 2060 3354 4418 5233 2060
Size: 1294 1064 815 4643
Fragments arranged by size:
4643 1294 1064 815
Bme1580I G_kGCm'C
Cuts at: 1790 2040 2204 2224 5877 7033 1790
Size: 250 164 20 3653 1156 2573
Fragments arranged by size:
3653 2573 1156 250 164 20
BmgBI CAC'GTC
Cuts at: 1318 2536 3229 1318
Size: 1218 693 5905
Fragments arranged by size:
5905 1218 693
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2207 3679 3709
Size: 416 53 83 399 24 790 1472 30
Cuts at: 3709 5015 5547 6505 442
Size: 1306 532 958 1753
Fragments arranged by size:
1753 1472 1306 958 790 532 416 399
83 53 30 24
BmtI G_CTAG'C
Cuts at: 1056 4743 1056
Size: 3687 4129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2108 2544 2600 2815 3563 4037 4048 4241
Size: 869 436 56 215 748 474 11 193
Cuts at: 4241 4520 6476 1239
Size: 279 1956 2579
Fragments arranged by size:
2579 1956 869 748 474 436 279 215
193 56 11
Bpu10I CC'TnA_GC
Cuts at: 2396 2908 3323 4149 4274 5049 2396
Size: 512 415 826 125 775 5163
Fragments arranged by size:
5163 826 775 512 415 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3372 3573 4259 4894 5941 6806 7104 3372
Size: 201 686 635 1047 865 298 4084
Fragments arranged by size:
4084 1047 865 686 635 298 201
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6479 882
Size: 617 4980 2219
Fragments arranged by size:
4980 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5607 493
Size: 711 4403 2702
Fragments arranged by size:
4403 2702 711
BsaBI GATnn'nnATC
Cuts at: 7810 7810
Size: 7816
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3915 4107 5623 6005
Size: 53 83 186 936 2382 192 1516 382
Cuts at: 6005 6640 275
Size: 635 1451
Fragments arranged by size:
2382 1516 1451 936 635 382 192 186
83 53
BsaWI w'CCGG_w
Cuts at: 6249 6647 6921 7068 7479 6249
Size: 398 274 147 411 6586
Fragments arranged by size:
6586 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3536 3566 3962 3992 4199
Size: 30 165 30 2133 30 396 30 207
Cuts at: 4199 4229 4641 4671 1178
Size: 30 412 30 4323
Fragments arranged by size:
4323 2133 412 396 207 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2300 3782 3930 3965 4313 4349 4424 4721
Size: 1114 1482 148 35 348 36 75 297
Cuts at: 4721 1186
Size: 4281
Fragments arranged by size:
4281 1482 1114 348 297 148 75 36
35
BseYI C'CCAG_C
Cuts at: 2081 2755 2871 3956 4339 4644 4749 4803 5055
Size: 674 116 1085 383 305 105 54 252
Cuts at: 5055 7019 7515 2081
Size: 1964 496 2382
Fragments arranged by size:
2382 1964 1085 674 496 383 305 252
116 105 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2440 1163
Size: 573 704 6539
Fragments arranged by size:
6539 704 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3285 5266 6027 7055 1540
Size: 275 186 1284 1981 761 1028 2301
Fragments arranged by size:
2301 1981 1284 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2277 3324 4305 4515 5877 5962 7033 2277
Size: 1047 981 210 1362 85 1071 3060
Fragments arranged by size:
3060 1362 1071 1047 981 210 85
BsmI GAATG_Cn'
Cuts at: 3475 4249 5327 5420 3475
Size: 774 1078 93 5871
Fragments arranged by size:
5871 1078 774 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2204 2224 2277 3324 4144 4305
Size: 399 250 164 20 53 1047 820 161
Cuts at: 4305 4515 5135 5877 5962 7033 1391
Size: 210 620 742 85 1071 2174
Fragments arranged by size:
2174 1071 1047 820 742 620 399 250
210 164 161 85 53 20
BspEI T'CCGG_A
Cuts at: 7479 7479
Size: 7816
BspHI T'CATG_A
Cuts at: 4726 5705 7435 4726
Size: 979 1730 5107
Fragments arranged by size:
5107 1730 979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2499 2847 3276 3465 3892 4139 5171
Size: 1179 476 348 429 189 427 247 1032
Cuts at: 5171 844
Size: 3489
Fragments arranged by size:
3489 1179 1032 476 429 427 348 247
189
BsrBI CCG'CTC
Cuts at: 1394 1823 2116 2634 2829 3733 4557 5703 1394
Size: 429 293 518 195 904 824 1146 3507
Fragments arranged by size:
3507 1146 904 824 518 429 293 195
BsrDI GCAATG_nn'
Cuts at: 65 1248 4110 6311 6485 65
Size: 1183 2862 2201 174 1396
Fragments arranged by size:
2862 2201 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2064 4844 6460 6647 1700
Size: 110 254 2780 1616 187 2869
Fragments arranged by size:
2869 2780 1616 254 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7816
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7816
BssSI C'ACGA_G
Cuts at: 5876 6888 5876
Size: 1012 6804
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3268 794
Size: 2474 5342
BstBI TT'CG_AA
Cuts at: 6620 6620
Size: 7816
BstEII G'GTnAC_C
Cuts at: 1184 3478 5101 1184
Size: 2294 1623 3899
Fragments arranged by size:
3899 2294 1623
BstXI CCAn_nnnn'nTGG
Cuts at: 4318 4639 4660 4318
Size: 321 21 7474
Fragments arranged by size:
7474 321 21
BstYI r'GATC_y
Cuts at: 1972 2815 2865 3247 4470 5146 5513 5898 5915
Size: 843 50 382 1223 676 367 385 17
Cuts at: 5915 7367 7453 7465 7811 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1223 843 676 385 382 367
346 86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3495 3981 4395 513
Size: 552 939 1491 486 414 3934
Fragments arranged by size:
3934 1491 939 552 486 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7077
Fragments arranged by size:
7077 683 56
BtsI GCAGTG_nn'
Cuts at: 2045 2277 2666 3079 4839 5087 5332 6126 6146
Size: 232 389 413 1760 248 245 794 20
Cuts at: 6146 2045
Size: 3715
Fragments arranged by size:
3715 1760 794 413 389 248 245 232
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7816
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7781
DraI TTT'AAA
Cuts at: 5120 5120
Size: 7816
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2233 2720 4445 6823 809
Size: 507 917 487 1725 2378 1802
Fragments arranged by size:
2378 1802 1725 917 507 487
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2410 3252
Size: 54 1425 50 275 18 186 394 842
Cuts at: 3252 3282 3498 3819 4368 4398 5263 6152 7781
Size: 30 216 321 549 30 865 889 1629
Cuts at: 7781 8
Size: 43
Fragments arranged by size:
1629 1425 889 865 842 549 394 321
275 216 186 54 50 43 30 30
18
EagI C'GGCC_G
Cuts at: 1537 1812 3282 5263 1537
Size: 275 1470 1981 4090
Fragments arranged by size:
4090 1981 1470 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2603 2738 3789 4221 4881 5746 1353
Size: 1250 135 1051 432 660 865 3423
Fragments arranged by size:
3423 1250 1051 865 660 432 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2111 6403 6777 6923 387
Size: 1064 138 522 4292 374 146 1280
Fragments arranged by size:
4292 1280 1064 522 374 146 138
EcoICRI GAG'CTC
Cuts at: 3322 4303 3322
Size: 981 6835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2108 2357 2544 2600 2815 3239 3563
Size: 393 476 249 187 56 215 424 324
Cuts at: 3563 3635 4037 4048 4241 4359 4520 5879 6476
Size: 72 402 11 193 118 161 1359 597
Cuts at: 6476 7263 7531 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1359 787 597 476 424 402 393
324 268 249 215 193 187 161 118
72 56 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2399 2987 4496 2399
Size: 588 1509 5719
Fragments arranged by size:
5719 1509 588
EcoO109I rG'GnC_Cy
Cuts at: 4345 4531 4345
Size: 186 7630
EcoRI G'AATT_C
Cuts at: 3296 5125 5503 3296
Size: 1829 378 5609
Fragments arranged by size:
5609 1829 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2749 3145 2749
Size: 396 7420
FspI TGC'GCA
Cuts at: 6322 6322
Size: 7816
HaeII r_GCGC'y
Cuts at: 1678 2082 6963 1678
Size: 404 4881 2531
Fragments arranged by size:
4881 2531 404
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2945 2977 3761 3793 4021
Size: 32 135 32 1373 32 784 32 228
Cuts at: 4021 4053 4308 4340 4641 4673 4936 4968 6457
Size: 32 255 32 301 32 263 32 1489
Cuts at: 6457 6489 6531 6563 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1489 1373 784 301 263 255 228
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2245 2556 5160 5406 669
Size: 988 281 307 311 2604 246 3079
Fragments arranged by size:
3079 2604 988 311 307 281 246
HindIII A'AGCT_T
Cuts at: 748 2726 748
Size: 1978 5838
HpaI GTT'AAC
Cuts at: 2556 5406 2556
Size: 2850 4966
KpnI G_GTAC'C
Cuts at: 5141 5141
Size: 7816
MfeI C'AATT_G
Cuts at: 5415 5415
Size: 7816
MluI A'CGCG_T
Cuts at: 7692 7692
Size: 7816
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3534 6930 7114 1305
Size: 2229 3396 184 2007
Fragments arranged by size:
3396 2229 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2018 2412 3500 3821 10
Size: 54 1425 343 186 394 1088 321 4005
Fragments arranged by size:
4005 1425 1088 394 343 321 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2709 3005 4259 4658 5774 6133 6292
Size: 648 1543 296 1254 399 1116 359 159
Cuts at: 6292 518
Size: 2042
Fragments arranged by size:
2042 1543 1254 1116 648 399 359 296
159
MspA1I CmG'CkG
Cuts at: 1606 2416 2512 2677 2759 2875 3202 3217 3334
Size: 810 96 165 82 116 327 15 117
Cuts at: 3334 3358 3580 3598 3951 4002 4379 4673 4807
Size: 24 222 18 353 51 377 294 134
Cuts at: 4807 5232 5909 7057 7302 1606
Size: 425 677 1148 245 2120
Fragments arranged by size:
2120 1148 810 677 425 377 353 327
294 245 222 165 134 117 116 96
82 51 24 18 15
NaeI GCC'GGC
Cuts at: 2066 4846 2066
Size: 2780 5036
NcoI C'CATG_G
Cuts at: 513 1065 2004 3495 513
Size: 552 939 1491 4834
Fragments arranged by size:
4834 1491 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7816
NgoMIV G'CCGG_C
Cuts at: 2064 4844 2064
Size: 2780 5036
NheI G'CTAG_C
Cuts at: 1052 4739 1052
Size: 3687 4129
NotI GC'GGCC_GC
Cuts at: 5263 5263
Size: 7816
NspI r_CATG'y
Cuts at: 1132 1229 2629 5174 6719 1132
Size: 97 1400 2545 1545 2229
Fragments arranged by size:
2545 2229 1545 1400 97
PasI CC'CwG_GG
Cuts at: 1348 4311 1348
Size: 2963 4853
PciI A'CATG_T
Cuts at: 2625 6715 2625
Size: 4090 3726
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4039 4756 4945 1108
Size: 2931 717 189 3979
Fragments arranged by size:
3979 2931 717 189
PfoI T'CCnGG_A
Cuts at: 3884 3884
Size: 7816
PmeI GTTT'AAAC
Cuts at: 5120 5120
Size: 7816
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2580 2612 3927 3959 5885 5917 7398 7430 2580
Size: 32 1315 32 1926 32 1481 32 2966
Fragments arranged by size:
2966 1926 1481 1315 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4345 4531 4345
Size: 186 7630
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7816
PsiI TTA'TAA
Cuts at: 5386 5386
Size: 7816
PstI C_TGCA'G
Cuts at: 830 2496 2702 2844 2953 3216 3801 3967 4812
Size: 1666 206 142 109 263 585 166 845
Cuts at: 4812 5168 830
Size: 356 3478
Fragments arranged by size:
3478 1666 845 585 356 263 206 166
142 109
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7816
PvuII CAG'CTG
Cuts at: 2416 2512 2759 2875 3217 3598 3951 4002 4379
Size: 96 247 116 342 381 353 51 377
Cuts at: 4379 4673 4807 2416
Size: 294 134 5425
Fragments arranged by size:
5425 381 377 353 342 294 247 134
116 96 51
SacI G_AGCT'C
Cuts at: 3324 4305 3324
Size: 981 6835
SalI G'TCGA_C
Cuts at: 1936 5158 1936
Size: 3222 4594
SapI GCTCTTCn'nnn_
Cuts at: 3789 3789
Size: 7816
SbfI CC_TGCA'GG
Cuts at: 5168 5168
Size: 7816
ScaI AGT'ACT
Cuts at: 2846 6064 7776 2846
Size: 3218 1712 2886
Fragments arranged by size:
3218 2886 1712
SexAI A'CCwGG_T
Cuts at: 4347 4347
Size: 7816
SfcI C'TryA_G
Cuts at: 826 1045 2492 2698 2840 2949 3212 3797 3963
Size: 219 1447 206 142 109 263 585 166
Cuts at: 3963 4808 5164 6299 6980 7171 826
Size: 845 356 1135 681 191 1471
Fragments arranged by size:
1471 1447 1135 845 681 585 356 263
219 206 191 166 142 109
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7816
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7816
SmaI CCC'GGG
Cuts at: 1872 5143 1872
Size: 3271 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3387 3588 4238 4909 5920 6821
Size: 197 926 1444 201 650 671 1011 901
Cuts at: 6821 7083 820
Size: 262 1553
Fragments arranged by size:
1553 1444 1011 926 901 671 650 262
201 197
SnaBI TAC'GTA
Cuts at: 493 5607 493
Size: 5114 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7816
SphI G_CATG'C
Cuts at: 1132 5174 1132
Size: 4042 3774
SspI AAT'ATT
Cuts at: 5 52 5740 5
Size: 47 5688 2081
Fragments arranged by size:
5688 2081 47
StuI AGG'CCT
Cuts at: 1517 2957 4175 4817 1517
Size: 1440 1218 642 4516
Fragments arranged by size:
4516 1440 1218 642
StyI C'CwwG_G
Cuts at: 513 1065 2004 2052 3495 4474 4632 4767 5255
Size: 552 939 48 1443 979 158 135 488
Cuts at: 5255 513
Size: 3074
Fragments arranged by size:
3074 1443 979 939 552 488 158 135
48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 2905 4184 5843 5860 6013 6198
Size: 1255 639 686 1279 1659 17 153 185
Cuts at: 6198 6643 325
Size: 445 1498
Fragments arranged by size:
1659 1498 1279 1255 686 639 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2844 3426 6062
Size: 274 80 33 51 1443 867 582 2636
Cuts at: 6062 7774 96
Size: 1712 138
Fragments arranged by size:
2636 1712 1443 867 582 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2455 2487 2880 2912 3824 3856 3962 3994 7776
Size: 32 393 32 912 32 106 32 3782
Cuts at: 7776 7808 2455
Size: 32 2463
Fragments arranged by size:
3782 2463 912 393 106 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7816
XbaI T'CTAG_A
Cuts at: 3607 5152 3607
Size: 1545 6271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4084 4660 2011
Size: 2073 576 5167
Fragments arranged by size:
5167 2073 576
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7816
XmaI C'CCGG_G
Cuts at: 1870 5141 1870
Size: 3271 4545
XmnI GAAnn'nnTTC
Cuts at: 5945 5945
Size: 7816
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5624 276
Size: 53 83 186 936 4090 2468
Fragments arranged by size:
4090 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI
FspAI KasI NarI NruI NsiI PacI PmlI PspOMI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI