Restriction map for
pFN21AB9460
(Circular) (Six-base) MAPSORT of: pFN21AB9460.seq Check: 9623 from: 1 to: 6889
pFN21AB9460 6889 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4699 278
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4232 1937
Size: 2295 4594
Acc65I G'GTAC_C
Cuts at: 4210 4210
Size: 6889
AclI AA'CG_TT
Cuts at: 1563 5016 5389 1563
Size: 3453 373 3063
Fragments arranged by size:
3453 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2852 4091 4952 6336 6604 1632
Size: 1220 1239 861 1384 268 1917
Fragments arranged by size:
1917 1384 1239 1220 861 268
AfeI AGC'GCT
Cuts at: 2150 2907 3527 2150
Size: 757 620 5512
Fragments arranged by size:
5512 757 620
AflII C'TTAA_G
Cuts at: 820 1017 3341 820
Size: 197 2324 4368
Fragments arranged by size:
4368 2324 197
AflIII A'CryG_T
Cuts at: 1203 5788 6765 1203
Size: 4585 977 1327
Fragments arranged by size:
4585 1327 977
AgeI A'CCGG_T
Cuts at: 5720 5720
Size: 6889
AhdI GACnn_n'nnGTC
Cuts at: 5618 5618
Size: 6889
AleI CACnn'nnGTG
Cuts at: 1166 2225 1166
Size: 1059 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2910 1184
Size: 1726 5163
AlwNI CAG_nnn'CTG
Cuts at: 2125 2689 3424 3640 4632 6204 2125
Size: 564 735 216 992 1572 2810
Fragments arranged by size:
2810 1572 992 735 564 216
ApaI G_GGCC'C
Cuts at: 2015 2244 2282 2317 2596 2616 2740 3649 4166
Size: 229 38 35 279 20 124 909 517
Cuts at: 4166 2015
Size: 4738
Fragments arranged by size:
4738 909 517 279 229 124 38 35
20
ApaLI G'TGCA_C
Cuts at: 4946 6102 4946
Size: 1156 5733
ApoI r'AATT_y
Cuts at: 1493 4198 4424 4576 6685 1493
Size: 2705 226 152 2109 1697
Fragments arranged by size:
2705 2109 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2585 2585
Size: 6889
AseI AT'TA_AT
Cuts at: 160 1032 5443 160
Size: 872 4411 1606
Fragments arranged by size:
4411 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
AvaI C'yCGr_G
Cuts at: 1870 1943 2348 2567 4159 4214 1870
Size: 73 405 219 1592 55 4545
Fragments arranged by size:
4545 1592 405 219 73 55
BamHI G'GATC_C
Cuts at: 3564 4219 4586 3564
Size: 655 367 5867
Fragments arranged by size:
5867 655 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 2744 3053 3332 3758
Size: 325 217 627 432 525 309 279 426
Cuts at: 3758 3929 4210 5665 618
Size: 171 281 1455 1842
Fragments arranged by size:
1842 1455 627 525 432 426 325 309
281 279 217 171
BanII G_rGCy'C
Cuts at: 1391 2015 2244 2282 2317 2596 2616 2740 2829
Size: 624 229 38 35 279 20 124 89
Cuts at: 2829 2969 3649 4050 4166 4208 1391
Size: 140 680 401 116 42 4072
Fragments arranged by size:
4072 680 624 401 279 229 140 124
116 89 42 38 35 20
BbeI G_GCGC'C
Cuts at: 2748 3336 3762 2748
Size: 588 426 5875
Fragments arranged by size:
5875 588 426
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3136 928
Size: 971 1237 4681
Fragments arranged by size:
4681 1237 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5080 5114 1903
Size: 34 3143 34 3678
Fragments arranged by size:
3678 3143 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4781 5991 4781
Size: 1210 5679
BclI T'GATC_A
Cuts at: 1548 3135 1548
Size: 1587 5302
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2019 2120 2277
Size: 107 122 71 631 605 347 101 157
Cuts at: 2277 2299 2620 3960 5500 136
Size: 22 321 1340 1540 1525
Fragments arranged by size:
1540 1525 1340 631 605 347 321 157
122 107 101 71 22
BglII A'GATC_T
Cuts at: 3471 6884 3471
Size: 3413 3476
BlpI GC'TnA_GC
Cuts at: 3911 4306 3911
Size: 395 6494
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2244 2282 2317 2596 2616 2740 3162
Size: 225 229 38 35 279 20 124 422
Cuts at: 3162 3649 4166 4950 6106 1790
Size: 487 517 784 1156 2573
Fragments arranged by size:
2573 1156 784 517 487 422 279 229
225 124 38 35 20
BmgBI CAC'GTC
Cuts at: 1318 2092 2488 1318
Size: 774 396 5719
Fragments arranged by size:
5719 774 396
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3007 3232 4142
Size: 416 53 83 399 24 1590 225 910
Cuts at: 4142 4620 5578 442
Size: 478 958 1753
Fragments arranged by size:
1753 1590 958 910 478 416 399 225
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6889
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 6889
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 2651 2666 3542 3650 5549 1239
Size: 854 558 15 876 108 1899 2579
Fragments arranged by size:
2579 1899 876 854 558 108 15
Bpu10I CC'TnA_GC
Cuts at: 2319 2480 2319
Size: 161 6728
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3480 3572 3677 5014 5879 6177 3480
Size: 92 105 1337 865 298 4192
Fragments arranged by size:
4192 1337 865 298 105 92
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3720 5552 882
Size: 617 2221 1832 2219
Fragments arranged by size:
2221 2219 1832 617
BsaAI yAC'GTr
Cuts at: 493 1204 3874 4680 493
Size: 711 2670 806 2702
Fragments arranged by size:
2702 2670 806 711
BsaBI GATnn'nnATC
Cuts at: 6883 6883
Size: 6889
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2745 3333 3759 4696
Size: 53 83 186 936 1212 588 426 937
Cuts at: 4696 5078 5713 275
Size: 382 635 1451
Fragments arranged by size:
1451 1212 937 936 635 588 426 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5322 5720 5994 6141 6552 5322
Size: 398 274 147 411 5659
Fragments arranged by size:
5659 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3959 3989 1178
Size: 30 165 30 2556 30 4078
Fragments arranged by size:
4078 2556 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3032 3257 3917 1186
Size: 1846 225 660 4158
Fragments arranged by size:
4158 1846 660 225
BseYI C'CCAG_C
Cuts at: 2125 2828 2902 3336 3787 6092 6588 2125
Size: 703 74 434 451 2305 496 2426
Fragments arranged by size:
2426 2305 703 496 451 434 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2636 4070 4130 1163
Size: 573 900 1434 60 3922
Fragments arranged by size:
3922 1434 900 573 60
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2063 2580 4339 5100 6128 1540
Size: 275 186 62 517 1759 761 1028 2301
Fragments arranged by size:
2301 1759 1028 761 517 275 186 62
BsiHKAI G_wGCw'C
Cuts at: 4950 5035 6106 4950
Size: 85 1071 5733
Fragments arranged by size:
5733 1071 85
BsmI GAATG_Cn'
Cuts at: 4400 4493 4400
Size: 93 6796
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2244 2282 2317 2596 2616 2740
Size: 399 225 229 38 35 279 20 124
Cuts at: 2740 2829 2969 3162 3649 4050 4166 4208 4950
Size: 89 140 193 487 401 116 42 742
Cuts at: 4950 5035 6106 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 487 401 399 279 229
225 193 140 124 116 89 85 42
38 35 20
BspEI T'CCGG_A
Cuts at: 6552 6552
Size: 6889
BspHI T'CATG_A
Cuts at: 4778 6508 4778
Size: 1730 5159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4244 844
Size: 3400 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2059 2557 3583 3915 4776 1394
Size: 429 236 498 1026 332 861 3507
Fragments arranged by size:
3507 1026 861 498 429 332 236
BsrDI GCAATG_nn'
Cuts at: 65 1248 3597 5384 5558 65
Size: 1183 2349 1787 174 1396
Fragments arranged by size:
2349 1787 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2623 2935 4028 5533 5720 1700
Size: 110 813 312 1093 1505 187 2869
Fragments arranged by size:
2869 1505 1093 813 312 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6889
BssHII G'CGCG_C
Cuts at: 1925 2585 2658 2770 2772 4153 1925
Size: 660 73 112 2 1381 4661
Fragments arranged by size:
4661 1381 660 112 73 2
BssSI C'ACGA_G
Cuts at: 2558 3224 3737 3854 4949 5961 2558
Size: 666 513 117 1095 1012 3486
Fragments arranged by size:
3486 1095 1012 666 513 117
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3640 4150 794
Size: 2846 510 3533
Fragments arranged by size:
3533 2846 510
BstBI TT'CG_AA
Cuts at: 5693 5693
Size: 6889
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6889
BstXI CCAn_nnnn'nTGG
Cuts at: 2950 3155 2950
Size: 205 6684
BstYI r'GATC_y
Cuts at: 1972 3084 3471 3564 3606 3720 4219 4586 4971
Size: 1112 387 93 42 114 499 367 385
Cuts at: 4971 4988 6440 6526 6538 6884 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1112 499 387 385 367 346
114 93 86 42 17 12
Bsu36I CC'TnA_GG
Cuts at: 2016 3110 2016
Size: 1094 5795
BtgI C'CryG_G
Cuts at: 513 1065 2004 2244 2273 2607 2616 2727 3978
Size: 552 939 240 29 334 9 111 1251
Cuts at: 3978 513
Size: 3424
Fragments arranged by size:
3424 1251 939 552 334 240 111 29
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6150
Fragments arranged by size:
6150 683 56
BtsI GCAGTG_nn'
Cuts at: 2288 2543 3333 4003 4405 5199 5219 2288
Size: 255 790 670 402 794 20 3958
Fragments arranged by size:
3958 794 790 670 402 255 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6854
DraI TTT'AAA
Cuts at: 4193 4193
Size: 6889
DraIII CAC_nnn'GTG
Cuts at: 4150 4150
Size: 6889
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3302 5896 809
Size: 507 1986 2594 1802
Fragments arranged by size:
2594 1986 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2060 2133 2577
Size: 54 1425 50 275 18 230 73 444
Cuts at: 2577 2952 4023 4336 5225 6854 8
Size: 375 1071 313 889 1629 43
Fragments arranged by size:
1629 1425 1071 889 444 375 313 275
230 73 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2060 2577 4336 1537
Size: 275 248 517 1759 4090
Fragments arranged by size:
4090 1759 517 275 248
EarI CTCTTCn'nnn_
Cuts at: 1353 2388 2877 3393 3474 4819 1353
Size: 1035 489 516 81 1345 3423
Fragments arranged by size:
3423 1345 1035 516 489 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 5476 5850 5996 387
Size: 1064 138 450 3437 374 146 1280
Fragments arranged by size:
3437 1280 1064 450 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 2651 2666 2852 3542 3650 4091
Size: 393 461 558 15 186 690 108 441
Cuts at: 4091 4952 5549 6336 6604 1239
Size: 861 597 787 268 1524
Fragments arranged by size:
1524 861 787 690 597 558 461 441
393 268 186 108 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2322 2600 2322
Size: 278 6611
EcoO109I rG'GnC_Cy
Cuts at: 2012 2068 2240 2278 2279 2301 2313 2314 2593
Size: 56 172 38 1 22 12 1 279
Cuts at: 2593 2612 2723 2737 4163 2012
Size: 19 111 14 1426 4738
Fragments arranged by size:
4738 1426 279 172 111 56 38 22
19 14 12 1 1
EcoRI G'AATT_C
Cuts at: 4198 4576 4198
Size: 378 6511
FspI TGC'GCA
Cuts at: 3716 5395 3716
Size: 1679 5210
HaeII r_GCGC'y
Cuts at: 1678 2126 2152 2630 2748 2811 2849 2909 3336
Size: 448 26 478 118 63 38 60 427
Cuts at: 3336 3529 3762 6036 1678
Size: 193 233 2274 2531
Fragments arranged by size:
2531 2274 478 448 427 233 193 118
63 60 38 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2417 2449 5530 5562 5604
Size: 32 135 32 845 32 3081 32 42
Cuts at: 5604 5636 1373
Size: 32 2626
Fragments arranged by size:
3081 2626 845 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4233 4479 669
Size: 988 281 2295 246 3079
Fragments arranged by size:
3079 2295 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2393 748
Size: 1645 5244
HpaI GTT'AAC
Cuts at: 4479 4479
Size: 6889
KasI G'GCGC_C
Cuts at: 2744 3332 3758 2744
Size: 588 426 5875
Fragments arranged by size:
5875 588 426
KpnI G_GTAC'C
Cuts at: 4214 4214
Size: 6889
MfeI C'AATT_G
Cuts at: 3444 4488 3444
Size: 1044 5845
MluI A'CGCG_T
Cuts at: 6765 6765
Size: 6889
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6003 6187 1305
Size: 4698 184 2007
Fragments arranged by size:
4698 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2954 10
Size: 54 1425 343 1122 3945
Fragments arranged by size:
3945 1425 1122 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2225 3153 4847 5206 5365 518
Size: 648 1059 928 1694 359 159 2042
Fragments arranged by size:
2042 1694 1059 928 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2609 2650 2729 2761 3640 3712 3958 4305
Size: 1003 41 79 32 879 72 246 347
Cuts at: 4305 4982 6130 6375 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1003 879 677 347 246 245
79 72 41 32
NaeI GCC'GGC
Cuts at: 2625 2937 2625
Size: 312 6577
NarI GG'CG_CC
Cuts at: 2745 3333 3759 2745
Size: 588 426 5875
Fragments arranged by size:
5875 588 426
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5398
Fragments arranged by size:
5398 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6889
NgoMIV G'CCGG_C
Cuts at: 2623 2935 2623
Size: 312 6577
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6889
NotI GC'GGCC_GC
Cuts at: 4336 4336
Size: 6889
NspI r_CATG'y
Cuts at: 1132 1229 4247 5792 1132
Size: 97 3018 1545 2229
Fragments arranged by size:
3018 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2719 2740 1348
Size: 1371 21 5497
Fragments arranged by size:
5497 1371 21
PciI A'CATG_T
Cuts at: 5788 5788
Size: 6889
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2689 1108
Size: 1581 5308
PfoI T'CCnGG_A
Cuts at: 3036 3036
Size: 6889
PmeI GTTT'AAAC
Cuts at: 4193 4193
Size: 6889
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4958 4990 6471 6503 4958
Size: 32 1481 32 5344
Fragments arranged by size:
5344 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2068 2723 2068
Size: 655 6234
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6889
PsiI TTA'TAA
Cuts at: 4459 4459
Size: 6889
PspOMI G'GGCC_C
Cuts at: 2011 2240 2278 2313 2592 2612 2736 3645 4162
Size: 229 38 35 279 20 124 909 517
Cuts at: 4162 2011
Size: 4738
Fragments arranged by size:
4738 909 517 279 229 124 38 35
20
PstI C_TGCA'G
Cuts at: 830 2982 3429 4241 830
Size: 2152 447 812 3478
Fragments arranged by size:
3478 2152 812 447
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6889
PvuII CAG'CTG
Cuts at: 3640 3712 3640
Size: 72 6817
RsrII CG'GwC_CG
Cuts at: 2731 2731
Size: 6889
SacII CC_GC'GG
Cuts at: 2610 2730 2610
Size: 120 6769
SalI G'TCGA_C
Cuts at: 1936 4231 1936
Size: 2295 4594
SanDI GG'GwC_CC
Cuts at: 2723 2723
Size: 6889
SapI GCTCTTCn'nnn_
Cuts at: 2877 3474 2877
Size: 597 6292
SbfI CC_TGCA'GG
Cuts at: 2982 4241 2982
Size: 1259 5630
ScaI AGT'ACT
Cuts at: 5137 6849 5137
Size: 1712 5177
SfcI C'TryA_G
Cuts at: 826 1045 2978 3425 4237 5372 6053 6244 826
Size: 219 1933 447 812 1135 681 191 1471
Fragments arranged by size:
1933 1471 1135 812 681 447 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2620 2620
Size: 6889
SfoI GGC'GCC
Cuts at: 2746 3334 3760 2746
Size: 588 426 5875
Fragments arranged by size:
5875 588 426
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6889
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6889
SmaI CCC'GGG
Cuts at: 1872 4161 4216 1872
Size: 2289 55 4545
Fragments arranged by size:
4545 2289 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3341 3495 3587 3692 4993 5894
Size: 197 926 1398 154 92 105 1301 901
Cuts at: 5894 6156 820
Size: 262 1553
Fragments arranged by size:
1553 1398 1301 926 901 262 197 154
105 92
SnaBI TAC'GTA
Cuts at: 493 4680 493
Size: 4187 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6889
SphI G_CATG'C
Cuts at: 1132 4247 1132
Size: 3115 3774
SspI AAT'ATT
Cuts at: 5 52 4813 5
Size: 47 4761 2081
Fragments arranged by size:
4761 2081 47
StuI AGG'CCT
Cuts at: 1517 2328 2573 3452 3653 1517
Size: 811 245 879 201 4753
Fragments arranged by size:
4753 879 811 245 201
StyI C'CwwG_G
Cuts at: 513 1065 2004 2925 4328 513
Size: 552 939 921 1403 3074
Fragments arranged by size:
3074 1403 939 921 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3764 4161 4916 4933 5086 5271 5716
Size: 1255 2184 397 755 17 153 185 445
Cuts at: 5716 325
Size: 1498
Fragments arranged by size:
2184 1498 1255 755 445 397 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3096 3240 3402
Size: 274 80 33 51 1443 1119 144 162
Cuts at: 3402 5135 6847 96
Size: 1733 1712 138
Fragments arranged by size:
1733 1712 1443 1119 274 162 144 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3007 6849 6881 2975
Size: 32 3842 32 2983
Fragments arranged by size:
3842 2983 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6889
XbaI T'CTAG_A
Cuts at: 4225 4225
Size: 6889
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6889
XmaI C'CCGG_G
Cuts at: 1870 4159 4214 1870
Size: 2289 55 4545
Fragments arranged by size:
4545 2289 55
XmnI GAAnn'nnTTC
Cuts at: 5018 5018
Size: 6889
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4697 276
Size: 53 83 186 936 3163 2468
Fragments arranged by size:
3163 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PspXI PsrI SacI SexAI SrfI SwaI
XcmI