Restriction map for
pFN21AB9459
(Circular) (Six-base) MAPSORT of: pFN21AB9459.seq Check: 8573 from: 1 to: 8377
pFN21AB9459 8377 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4405 4405
Size: 8377
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6187 278
Size: 53 83 186 936 4651 2468
Fragments arranged by size:
4651 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3738 5720 1937
Size: 1801 1982 4594
Fragments arranged by size:
4594 1982 1801
Acc65I G'GTAC_C
Cuts at: 5698 5698
Size: 8377
AclI AA'CG_TT
Cuts at: 1563 6504 6877 1563
Size: 4941 373 3063
Fragments arranged by size:
4941 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3249 3344 3930 4271 4731 5000 5027 6440
Size: 1617 95 586 341 460 269 27 1413
Cuts at: 6440 7824 8092 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1617 1413 1384 586 460 341 269
268 95 27
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8180
AflIII A'CryG_T
Cuts at: 1203 3501 3508 7276 8253 1203
Size: 2298 7 3768 977 1327
Fragments arranged by size:
3768 2298 1327 977 7
AgeI A'CCGG_T
Cuts at: 7208 7208
Size: 8377
AhdI GACnn_n'nnGTC
Cuts at: 7106 7106
Size: 8377
AleI CACnn'nnGTG
Cuts at: 1166 2619 3719 3964 5618 1166
Size: 1453 1100 245 1654 3925
Fragments arranged by size:
3925 1654 1453 1100 245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3413 1184
Size: 2229 6148
AlwNI CAG_nnn'CTG
Cuts at: 2197 4921 6120 7692 2197
Size: 2724 1199 1572 2882
Fragments arranged by size:
2882 2724 1572 1199
ApaI G_GGCC'C
Cuts at: 5403 5403
Size: 8377
ApaLI G'TGCA_C
Cuts at: 2125 5300 6434 7590 2125
Size: 3175 1134 1156 2912
Fragments arranged by size:
3175 2912 1156 1134
ApoI r'AATT_y
Cuts at: 1493 2015 3797 5066 5087 5686 5912 6064 8173
Size: 522 1782 1269 21 599 226 152 2109
Cuts at: 8173 1493
Size: 1697
Fragments arranged by size:
2109 1782 1697 1269 599 522 226 152
21
AseI AT'TA_AT
Cuts at: 160 1032 6931 160
Size: 872 5899 1606
Fragments arranged by size:
5899 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
AvaI C'yCGr_G
Cuts at: 1870 1943 3765 4896 5396 5702 1870
Size: 73 1822 1131 500 306 4545
Fragments arranged by size:
4545 1822 1131 500 306 73
BamHI G'GATC_C
Cuts at: 2073 4245 5707 6074 2073
Size: 2172 1462 367 4376
Fragments arranged by size:
4376 2172 1462 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3352 4463 5382 5698 7153
Size: 325 217 627 1565 1111 919 316 1455
Cuts at: 7153 618
Size: 1842
Fragments arranged by size:
1842 1565 1455 1111 919 627 325 316
217
BanII G_rGCy'C
Cuts at: 1391 2049 4668 5403 5696 1391
Size: 658 2619 735 293 4072
Fragments arranged by size:
4072 2619 735 658 293
BbeI G_GCGC'C
Cuts at: 5386 5386
Size: 8377
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2451 2764 928
Size: 971 552 313 6541
Fragments arranged by size:
6541 971 552 313
BbvCI CC'TCA_GC
Cuts at: 2798 2798
Size: 8377
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6568 6602 1903
Size: 34 4631 34 3678
Fragments arranged by size:
4631 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4411 4453 6269 7479 4411
Size: 42 1816 1210 5309
Fragments arranged by size:
5309 1816 1210 42
BclI T'GATC_A
Cuts at: 1548 2344 3312 3813 4789 1548
Size: 796 968 501 976 5136
Fragments arranged by size:
5136 976 968 796 501
BfrBI ATG'CAT
Cuts at: 3301 3301
Size: 8377
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6988 136
Size: 107 122 71 631 605 5316 1525
Fragments arranged by size:
5316 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2694 3369 4657 4758 8372 2694
Size: 675 1288 101 3614 2699
Fragments arranged by size:
3614 2699 1288 675 101
BlpI GC'TnA_GC
Cuts at: 2048 5794 2048
Size: 3746 4631
Bme1580I G_kGCm'C
Cuts at: 1790 2129 2280 2406 3357 4731 5304 5403 6438
Size: 339 151 126 951 1374 573 99 1035
Cuts at: 6438 7594 1790
Size: 1156 2573
Fragments arranged by size:
2573 1374 1156 1035 951 573 339 151
126 99
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8377
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2568 2641 2859
Size: 416 53 83 399 24 1151 73 218
Cuts at: 2859 3678 6108 7066 442
Size: 819 2430 958 1753
Fragments arranged by size:
2430 1753 1151 958 819 416 399 218
83 73 53 24
BmtI G_CTAG'C
Cuts at: 1056 3156 1056
Size: 2100 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2229 3875 3983 4373 4682 7037 1239
Size: 990 1646 108 390 309 2355 2579
Fragments arranged by size:
2579 2355 1646 990 390 309 108
Bpu10I CC'TnA_GC
Cuts at: 2798 4674 2798
Size: 1876 6501
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5333 6502 7367 7665 5333
Size: 1169 865 298 6045
Fragments arranged by size:
6045 1169 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2015 2865 7040 882
Size: 617 516 850 4175 2219
Fragments arranged by size:
4175 2219 850 617 516
BsaAI yAC'GTr
Cuts at: 493 1204 6168 493
Size: 711 4964 2702
Fragments arranged by size:
4964 2702 711
BsaBI GATnn'nnATC
Cuts at: 8371 8371
Size: 8377
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5383 6184 6566 7201
Size: 53 83 186 936 3850 801 382 635
Cuts at: 7201 275
Size: 1451
Fragments arranged by size:
3850 1451 936 801 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6810 7208 7482 7629 8040 6810
Size: 398 274 147 411 7147
Fragments arranged by size:
7147 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8152
Fragments arranged by size:
8152 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2226 4503 5087 1186
Size: 1040 2277 584 4476
Fragments arranged by size:
4476 2277 1040 584
BseYI C'CCAG_C
Cuts at: 4023 4033 4536 4641 5154 7580 8076 4023
Size: 10 503 105 513 2426 496 4324
Fragments arranged by size:
4324 2426 513 503 496 105 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3824 4383 1163
Size: 573 2088 559 5157
Fragments arranged by size:
5157 2088 573 559
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2620 5827 6588 7616 1540
Size: 275 186 619 3207 761 1028 2301
Fragments arranged by size:
3207 2301 1028 761 619 275 186
BsiHKAI G_wGCw'C
Cuts at: 2129 2428 4495 4668 5304 6438 6523 7594 2129
Size: 299 2067 173 636 1134 85 1071 2912
Fragments arranged by size:
2912 2067 1134 1071 636 299 173 85
BsmI GAATG_Cn'
Cuts at: 3301 3859 4213 5888 5981 3301
Size: 558 354 1675 93 5697
Fragments arranged by size:
5697 1675 558 354 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2209 2209
Size: 8377
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2129 2280 2406 2428 3357 4495
Size: 399 259 80 151 126 22 929 1138
Cuts at: 4495 4668 4731 5304 5403 5696 6438 6523 7594
Size: 173 63 573 99 293 742 85 1071
Cuts at: 7594 1391
Size: 2174
Fragments arranged by size:
2174 1138 1071 929 742 573 399 293
259 173 151 126 99 85 80 63
22
BspEI T'CCGG_A
Cuts at: 8040 8040
Size: 8377
BspHI T'CATG_A
Cuts at: 2311 3577 4834 6266 7996 2311
Size: 1266 1257 1432 1730 2692
Fragments arranged by size:
2692 1730 1432 1266 1257
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2744 4405 5298 5732 844
Size: 1900 1661 893 434 3489
Fragments arranged by size:
3489 1900 1661 893 434
BsrBI CCG'CTC
Cuts at: 1394 1823 6264 1394
Size: 429 4441 3507
Fragments arranged by size:
4441 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2267 4467 6872 7046 65
Size: 1183 1019 2200 2405 174 1396
Fragments arranged by size:
2405 2200 1396 1183 1019 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7021 7208 1700
Size: 110 5211 187 2869
Fragments arranged by size:
5211 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3143 3183 96
Size: 3047 40 5290
Fragments arranged by size:
5290 3047 40
BssHII G'CGCG_C
Cuts at: 1925 2031 1925
Size: 106 8271
BssSI C'ACGA_G
Cuts at: 6437 7449 6437
Size: 1012 7365
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3128 4499 5299 794
Size: 2334 1371 800 3872
Fragments arranged by size:
3872 2334 1371 800
BstBI TT'CG_AA
Cuts at: 3688 4812 7181 3688
Size: 1124 2369 4884
Fragments arranged by size:
4884 2369 1124
BstEII G'GTnAC_C
Cuts at: 1184 5611 1184
Size: 4427 3950
BstXI CCAn_nnnn'nTGG
Cuts at: 2939 4006 2939
Size: 1067 7310
BstYI r'GATC_y
Cuts at: 1972 2073 2694 3369 4245 4657 4758 5707 6074
Size: 101 621 675 876 412 101 949 367
Cuts at: 6074 6459 6476 7928 8014 8026 8372 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 949 876 675 621 412 385
367 346 101 101 86 17 12
BstZ17I GTA'TAC
Cuts at: 3739 3739
Size: 8377
Bsu36I CC'TnA_GG
Cuts at: 3244 5409 3244
Size: 2165 6212
BtgI C'CryG_G
Cuts at: 513 1065 3676 4297 5199 5536 513
Size: 552 2611 621 902 337 3354
Fragments arranged by size:
3354 2611 902 621 552 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7638
Fragments arranged by size:
7638 683 56
BtsI GCAGTG_nn'
Cuts at: 2595 3917 5893 6687 6707 2595
Size: 1322 1976 794 20 4265
Fragments arranged by size:
4265 1976 1322 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8377
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8342
DraI TTT'AAA
Cuts at: 5681 5681
Size: 8377
DraIII CAC_nnn'GTG
Cuts at: 3847 5567 3847
Size: 1720 6657
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7384 809
Size: 507 6068 1802
Fragments arranged by size:
6068 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2972 3868 4195
Size: 54 1425 50 275 18 1142 896 327
Cuts at: 4195 5824 6713 8342 8
Size: 1629 889 1629 43
Fragments arranged by size:
1629 1629 1425 1142 896 889 327 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5824 1537
Size: 275 4012 4090
Fragments arranged by size:
4090 4012 275
EarI CTCTTCn'nnn_
Cuts at: 1353 6307 1353
Size: 4954 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6964 7338 7484 387
Size: 1064 138 5375 374 146 1280
Fragments arranged by size:
5375 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4666 4666
Size: 8377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2229 3249 3344 3875 3930 3983 4271
Size: 393 597 1020 95 531 55 53 288
Cuts at: 4271 4373 4682 4731 5000 5027 6440 7037 7824
Size: 102 309 49 269 27 1413 597 787
Cuts at: 7824 8092 1239
Size: 268 1524
Fragments arranged by size:
1524 1413 1020 787 597 597 531 393
309 288 269 268 102 95 55 53
49 27
EcoNI CCTnn'n_nnAGG
Cuts at: 4052 4052
Size: 8377
EcoO109I rG'GnC_Cy
Cuts at: 2890 3603 5571 2890
Size: 713 1968 5696
Fragments arranged by size:
5696 1968 713
EcoRI G'AATT_C
Cuts at: 5686 6064 5686
Size: 378 7999
FspI TGC'GCA
Cuts at: 6883 6883
Size: 8377
HaeII r_GCGC'y
Cuts at: 1678 2257 5386 7524 1678
Size: 579 3129 2138 2531
Fragments arranged by size:
3129 2531 2138 579
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2058 2090 2113 2145 2410
Size: 32 135 32 486 32 23 32 265
Cuts at: 2410 2442 3191 3223 3304 3336 3398 3430 5647
Size: 32 749 32 81 32 62 32 2217
Cuts at: 5647 5679 7018 7050 7092 7124 1373
Size: 32 1339 32 42 32 2626
Fragments arranged by size:
2626 2217 1339 749 486 265 135 81
62 42 32 32 32 32 32 32
32 32 32 32 32 23
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2397 5721 5967 669
Size: 988 281 72 387 3324 246 3079
Fragments arranged by size:
3324 3079 988 387 281 246 72
HindIII A'AGCT_T
Cuts at: 748 2246 5009 748
Size: 1498 2763 4116
Fragments arranged by size:
4116 2763 1498
HpaI GTT'AAC
Cuts at: 2010 2397 5967 2010
Size: 387 3570 4420
Fragments arranged by size:
4420 3570 387
KasI G'GCGC_C
Cuts at: 5382 5382
Size: 8377
KpnI G_GTAC'C
Cuts at: 5702 5702
Size: 8377
MfeI C'AATT_G
Cuts at: 4423 5976 4423
Size: 1553 6824
MluI A'CGCG_T
Cuts at: 8253 8253
Size: 8377
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4542 4775 5050 7491 7675 1305
Size: 3237 233 275 2441 184 2007
Fragments arranged by size:
3237 2441 2007 275 233 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3870 4197 10
Size: 54 1425 343 2038 327 4190
Fragments arranged by size:
4190 2038 1425 343 327 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2619 2937 3719 3964 5618 6335
Size: 648 1191 262 318 782 245 1654 717
Cuts at: 6335 6694 6853 518
Size: 359 159 2042
Fragments arranged by size:
2042 1654 1191 782 717 648 359 318
262 245 159
MspA1I CmG'CkG
Cuts at: 1606 3034 4289 4700 4997 5038 5366 5793 6470
Size: 1428 1255 411 297 41 328 427 677
Cuts at: 6470 7618 7863 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1428 1255 1148 677 427 411 328
297 245 41
NarI GG'CG_CC
Cuts at: 5383 5383
Size: 8377
NcoI C'CATG_G
Cuts at: 513 1065 4297 513
Size: 552 3232 4593
Fragments arranged by size:
4593 3232 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8377
NheI G'CTAG_C
Cuts at: 1052 3152 1052
Size: 2100 6277
NotI GC'GGCC_GC
Cuts at: 5824 5824
Size: 8377
NsiI A_TGCA'T
Cuts at: 3303 3303
Size: 8377
NspI r_CATG'y
Cuts at: 1132 1229 2603 3505 3512 5491 5735 7280 1132
Size: 97 1374 902 7 1979 244 1545 2229
Fragments arranged by size:
2229 1979 1545 1374 902 244 97 7
PasI CC'CwG_GG
Cuts at: 1348 4691 1348
Size: 3343 5034
PciI A'CATG_T
Cuts at: 3501 3508 7276 3501
Size: 7 3768 4602
Fragments arranged by size:
4602 3768 7
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4072 5567 1108
Size: 2964 1495 3918
Fragments arranged by size:
3918 2964 1495
PmeI GTTT'AAAC
Cuts at: 5681 5681
Size: 8377
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6446 6478 7959 7991 6446
Size: 32 1481 32 6832
Fragments arranged by size:
6832 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2890 2890
Size: 8377
PshAI GACnn'nnGTC
Cuts at: 1613 4267 1613
Size: 2654 5723
PsiI TTA'TAA
Cuts at: 5947 5947
Size: 8377
PspOMI G'GGCC_C
Cuts at: 5399 5399
Size: 8377
PstI C_TGCA'G
Cuts at: 830 4056 4560 5295 5609 5670 5729 830
Size: 3226 504 735 314 61 59 3478
Fragments arranged by size:
3478 3226 735 504 314 61 59
PvuI CG_AT'CG
Cuts at: 2001 2620 2001
Size: 619 7758
PvuII CAG'CTG
Cuts at: 3034 4289 4700 4997 5038 5366 3034
Size: 1255 411 297 41 328 6045
Fragments arranged by size:
6045 1255 411 328 297 41
SacI G_AGCT'C
Cuts at: 4668 4668
Size: 8377
SalI G'TCGA_C
Cuts at: 1936 5719 1936
Size: 3783 4594
SbfI CC_TGCA'GG
Cuts at: 5729 5729
Size: 8377
ScaI AGT'ACT
Cuts at: 6625 8337 6625
Size: 1712 6665
SfcI C'TryA_G
Cuts at: 826 1045 2580 4052 4556 4587 4883 5291 5605
Size: 219 1535 1472 504 31 296 408 314
Cuts at: 5605 5666 5725 6860 7541 7732 826
Size: 61 59 1135 681 191 1471
Fragments arranged by size:
1535 1472 1471 1135 681 504 408 314
296 219 191 61 59 31
SfoI GGC'GCC
Cuts at: 5384 5384
Size: 8377
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8377
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8377
SmaI CCC'GGG
Cuts at: 1872 3767 5704 1872
Size: 1895 1937 4545
Fragments arranged by size:
4545 1937 1895
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5348 6481 7382 7644 820
Size: 197 926 3405 1133 901 262 1553
Fragments arranged by size:
3405 1553 1133 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6168 493
Size: 5675 2702
SpeI A'CTAG_T
Cuts at: 152 2081 3862 152
Size: 1929 1781 4667
Fragments arranged by size:
4667 1929 1781
SphI G_CATG'C
Cuts at: 1132 5735 1132
Size: 4603 3774
SspI AAT'ATT
Cuts at: 5 52 6301 5
Size: 47 6249 2081
Fragments arranged by size:
6249 2081 47
StuI AGG'CCT
Cuts at: 1517 3249 1517
Size: 1732 6645
StyI C'CwwG_G
Cuts at: 513 1065 2069 4297 4737 5816 513
Size: 552 1004 2228 440 1079 3074
Fragments arranged by size:
3074 2228 1079 1004 552 440
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3033 4211 5294 6404 6421 6574 6759
Size: 1255 1453 1178 1083 1110 17 153 185
Cuts at: 6759 7204 325
Size: 445 1498
Fragments arranged by size:
1498 1453 1255 1178 1110 1083 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2602 3143 3183
Size: 274 80 33 51 1443 625 541 40
Cuts at: 3183 5181 6623 8335 96
Size: 1998 1442 1712 138
Fragments arranged by size:
1998 1712 1443 1442 625 541 274 138
80 51 40 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2317 2349 3766 3798 8337 8369 2317
Size: 32 1417 32 4539 32 2325
Fragments arranged by size:
4539 2325 1417 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8377
XbaI T'CTAG_A
Cuts at: 2375 3160 5236 5713 2375
Size: 785 2076 477 5039
Fragments arranged by size:
5039 2076 785 477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 4758 5410 2446
Size: 2312 652 5413
Fragments arranged by size:
5413 2312 652
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8377
XmaI C'CCGG_G
Cuts at: 1870 3765 5702 1870
Size: 1895 1937 4545
Fragments arranged by size:
4545 1937 1895
XmnI GAAnn'nnTTC
Cuts at: 4149 6506 4149
Size: 2357 6020
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6185 276
Size: 53 83 186 936 4651 2468
Fragments arranged by size:
4651 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BplI BsiWI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI
PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SrfI SwaI