Restriction map for
pFN21AB9458
(Circular) (Six-base) MAPSORT of: pFN21AB9458.seq Check: 1417 from: 1 to: 7216
pFN21AB9458 7216 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3176 3908 4171 3176
Size: 732 263 6221
Fragments arranged by size:
6221 732 263
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2682 5026 278
Size: 53 83 186 936 1146 2344 2468
Fragments arranged by size:
2468 2344 1146 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3321 3456 3747 4559 1937
Size: 1384 135 291 812 4594
Fragments arranged by size:
4594 1384 812 291 135
Acc65I G'GTAC_C
Cuts at: 4537 4537
Size: 7216
AclI AA'CG_TT
Cuts at: 1563 5343 5716 1563
Size: 3780 373 3063
Fragments arranged by size:
3780 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2579 2990 3574 4210 4484 5279 6663 6931
Size: 947 411 584 636 274 795 1384 268
Cuts at: 6931 1632
Size: 1917
Fragments arranged by size:
1917 1384 947 795 636 584 411 274
268
AfeI AGC'GCT
Cuts at: 2391 2391
Size: 7216
AflII C'TTAA_G
Cuts at: 820 1017 4139 820
Size: 197 3122 3897
Fragments arranged by size:
3897 3122 197
AflIII A'CryG_T
Cuts at: 1203 6115 7092 1203
Size: 4912 977 1327
Fragments arranged by size:
4912 1327 977
AgeI A'CCGG_T
Cuts at: 2203 4215 6047 2203
Size: 2012 1832 3372
Fragments arranged by size:
3372 2012 1832
AhdI GACnn_n'nnGTC
Cuts at: 5945 5945
Size: 7216
AleI CACnn'nnGTG
Cuts at: 1166 3578 3779 4265 1166
Size: 2412 201 486 4117
Fragments arranged by size:
4117 2412 486 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2876 4193 1184
Size: 1692 1317 4207
Fragments arranged by size:
4207 1692 1317
AlwNI CAG_nnn'CTG
Cuts at: 2316 2453 3559 4359 4959 6531 2316
Size: 137 1106 800 600 1572 3001
Fragments arranged by size:
3001 1572 1106 800 600 137
ApaI G_GGCC'C
Cuts at: 2410 3129 2410
Size: 719 6497
ApaLI G'TGCA_C
Cuts at: 2770 3131 5273 6429 2770
Size: 361 2142 1156 3557
Fragments arranged by size:
3557 2142 1156 361
ApoI r'AATT_y
Cuts at: 1493 4525 4751 4903 7012 1493
Size: 3032 226 152 2109 1697
Fragments arranged by size:
3032 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5770 160
Size: 872 4738 1606
Fragments arranged by size:
4738 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
AvaI C'yCGr_G
Cuts at: 1870 1943 2398 3080 4288 4404 4427 4541 1870
Size: 73 455 682 1208 116 23 114 4545
Fragments arranged by size:
4545 1208 682 455 116 114 73 23
BamHI G'GATC_C
Cuts at: 4546 4913 4546
Size: 367 6849
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2206 3090 4537 5992 618
Size: 325 217 627 419 884 1447 1455 1842
Fragments arranged by size:
1842 1455 1447 884 627 419 325 217
BanII G_rGCy'C
Cuts at: 1391 2399 2410 2985 3081 3129 4535 1391
Size: 1008 11 575 96 48 1406 4072
Fragments arranged by size:
4072 1406 1008 575 96 48 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2257 2471 3515 3748 3804 3967 4290
Size: 971 358 214 1044 233 56 163 323
Cuts at: 4290 4477 928
Size: 187 3667
Fragments arranged by size:
3667 1044 971 358 323 233 214 187
163 56
BbvCI CC'TCA_GC
Cuts at: 4479 4479
Size: 7216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5407 5441 1903
Size: 34 3470 34 3678
Fragments arranged by size:
3678 3470 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5108 6318 5108
Size: 1210 6006
BclI T'GATC_A
Cuts at: 1548 3336 1548
Size: 1788 5428
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5827 136
Size: 107 122 71 631 605 4155 1525
Fragments arranged by size:
4155 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7211 7211
Size: 7216
BlpI GC'TnA_GC
Cuts at: 4422 4633 4422
Size: 211 7005
Bme1580I G_kGCm'C
Cuts at: 1790 2410 2774 3129 3135 5277 6433 1790
Size: 620 364 355 6 2142 1156 2573
Fragments arranged by size:
2573 2142 1156 620 364 355 6
BmgBI CAC'GTC
Cuts at: 1318 3834 1318
Size: 2516 4700
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4947 5905 442
Size: 416 53 83 399 24 3530 958 1753
Fragments arranged by size:
3530 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7216
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2157 3196 2157
Size: 1039 6177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2004 2015 2338 2552 2996 3044 3209 3426
Size: 765 11 323 214 444 48 165 217
Cuts at: 3426 3447 3926 3962 5876 1239
Size: 21 479 36 1914 2579
Fragments arranged by size:
2579 1914 765 479 444 323 217 214
165 48 36 21 11
Bpu10I CC'TnA_GC
Cuts at: 4165 4319 4479 4165
Size: 154 160 6902
Fragments arranged by size:
6902 160 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3584 5341 6206 6504 3584
Size: 1757 865 298 4296
Fragments arranged by size:
4296 1757 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 3209 3717 4464 5879 882
Size: 617 504 1206 508 747 1415 2219
Fragments arranged by size:
2219 1415 1206 747 617 508 504
BsaAI yAC'GTr
Cuts at: 493 1204 5007 493
Size: 711 3803 2702
Fragments arranged by size:
3803 2702 711
BsaBI GATnn'nnATC
Cuts at: 2939 3686 7210 2939
Size: 747 3524 2945
Fragments arranged by size:
3524 2945 747
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2679 3005 4305 5023
Size: 53 83 186 936 1146 326 1300 718
Cuts at: 5023 5405 6040 275
Size: 382 635 1451
Fragments arranged by size:
1451 1300 1146 936 718 635 382 326
186 83 53
BsaWI w'CCGG_w
Cuts at: 2203 4215 5649 6047 6321 6468 6879 2203
Size: 2012 1434 398 274 147 411 2540
Fragments arranged by size:
2540 2012 1434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2002 2032 2664 2694 3068
Size: 30 165 30 599 30 632 30 374
Cuts at: 3068 3098 1178
Size: 30 5296
Fragments arranged by size:
5296 632 599 374 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2110 2993 3127 3465 1186
Size: 924 883 134 338 4937
Fragments arranged by size:
4937 924 883 338 134
BseYI C'CCAG_C
Cuts at: 2042 2386 2392 2410 2782 3023 6419 6915 2042
Size: 344 6 18 372 241 3396 496 2343
Fragments arranged by size:
3396 2343 496 372 344 241 18 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2480 2600 2849 2942 3106 1163
Size: 573 744 120 249 93 164 5273
Fragments arranged by size:
5273 744 573 249 164 120 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2826 2898 3500 4666 5427 6455
Size: 275 186 825 72 602 1166 761 1028
Cuts at: 6455 1540
Size: 2301
Fragments arranged by size:
2301 1166 1028 825 761 602 275 186
72
BsiHKAI G_wGCw'C
Cuts at: 2774 2985 3135 4405 5277 5362 6433 2774
Size: 211 150 1270 872 85 1071 3557
Fragments arranged by size:
3557 1270 1071 872 211 150 85
BsmI GAATG_Cn'
Cuts at: 4727 4820 4727
Size: 93 7123
BsmBI CGTCTCn'nnnn_
Cuts at: 2186 2626 2186
Size: 440 6776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2399 2410 2774 2985 3081 3129 3135
Size: 399 609 11 364 211 96 48 6
Cuts at: 3135 4405 4535 5277 5362 6433 1391
Size: 1270 130 742 85 1071 2174
Fragments arranged by size:
2174 1270 1071 742 609 399 364 211
130 96 85 48 11 6
BspEI T'CCGG_A
Cuts at: 6879 6879
Size: 7216
BspHI T'CATG_A
Cuts at: 3699 5105 6835 3699
Size: 1406 1730 4080
Fragments arranged by size:
4080 1730 1406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2502 3176 3908 4171 4571 844
Size: 1658 674 732 263 400 3489
Fragments arranged by size:
3489 1658 732 674 400 263
BsrBI CCG'CTC
Cuts at: 1394 1823 4164 5103 1394
Size: 429 2341 939 3507
Fragments arranged by size:
3507 2341 939 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4035 5711 5885 65
Size: 1183 2787 1676 174 1396
Fragments arranged by size:
2787 1676 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2203 4215 5860 6047 1700
Size: 110 393 2012 1645 187 2869
Fragments arranged by size:
2869 2012 1645 393 187 110
BsrGI T'GTAC_A
Cuts at: 96 2925 3330 96
Size: 2829 405 3982
Fragments arranged by size:
3982 2829 405
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7216
BssSI C'ACGA_G
Cuts at: 2122 5276 6288 2122
Size: 3154 1012 3050
Fragments arranged by size:
3154 3050 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7216
BstBI TT'CG_AA
Cuts at: 6020 6020
Size: 7216
BstEII G'GTnAC_C
Cuts at: 1184 2650 1184
Size: 1466 5750
BstXI CCAn_nnnn'nTGG
Cuts at: 2782 3781 3904 3931 2782
Size: 999 123 27 6067
Fragments arranged by size:
6067 999 123 27
BstYI r'GATC_y
Cuts at: 1972 2313 3069 3432 4546 4913 5298 5315 6767
Size: 341 756 363 1114 367 385 17 1452
Cuts at: 6767 6853 6865 7211 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1114 756 385 367 363 346
341 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2257 2257
Size: 7216
BtgI C'CryG_G
Cuts at: 513 1065 2004 2051 2695 3348 3369 3576 3618
Size: 552 939 47 644 653 21 207 42
Cuts at: 3618 3667 513
Size: 49 4062
Fragments arranged by size:
4062 939 653 644 552 207 49 47
42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2605 487
Size: 683 56 1379 5098
Fragments arranged by size:
5098 1379 683 56
BtsI GCAGTG_nn'
Cuts at: 2612 4732 5526 5546 2612
Size: 2120 794 20 4282
Fragments arranged by size:
4282 2120 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7216
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7181
DraI TTT'AAA
Cuts at: 4520 4520
Size: 7216
DraIII CAC_nnn'GTG
Cuts at: 3114 3114
Size: 7216
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3287 4481 6223 809
Size: 507 1971 1194 1742 1802
Fragments arranged by size:
1971 1802 1742 1194 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2054 2763 2823
Size: 54 1425 50 275 18 224 709 60
Cuts at: 2823 2883 2895 3497 3570 4663 5552 7181 8
Size: 60 12 602 73 1093 889 1629 43
Fragments arranged by size:
1629 1425 1093 889 709 602 275 224
73 60 60 54 50 43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 2823 2895 3497 4663 1537
Size: 275 1011 72 602 1166 4090
Fragments arranged by size:
4090 1166 1011 602 275 72
EarI CTCTTCn'nnn_
Cuts at: 1353 2338 4124 5146 1353
Size: 985 1786 1022 3423
Fragments arranged by size:
3423 1786 1022 985
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5803 6177 6323 387
Size: 1064 138 4214 374 146 1280
Fragments arranged by size:
4214 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2983 2983
Size: 7216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2015 2338 2552 2579 2990 2996
Size: 393 372 11 323 214 27 411 6
Cuts at: 2996 3044 3209 3426 3447 3574 3926 3962 4210
Size: 48 165 217 21 127 352 36 248
Cuts at: 4210 4484 5279 5876 6663 6931 1239
Size: 274 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 411 393 372 352
323 274 268 248 217 214 165 127
48 36 27 21 11 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2457 2876 3361 2457
Size: 419 485 6312
Fragments arranged by size:
6312 485 419
EcoO109I rG'GnC_Cy
Cuts at: 2406 2407 3851 3864 2406
Size: 1 1444 13 5758
Fragments arranged by size:
5758 1444 13 1
EcoRI G'AATT_C
Cuts at: 4525 4903 4525
Size: 378 6838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3316 3991 3316
Size: 675 6541
FspI TGC'GCA
Cuts at: 5722 5722
Size: 7216
HaeII r_GCGC'y
Cuts at: 1678 2393 2838 6363 1678
Size: 715 445 3525 2531
Fragments arranged by size:
3525 2531 715 445
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2002 2034 2664 2696 3208
Size: 32 135 32 430 32 630 32 512
Cuts at: 3208 3240 3422 3454 3818 3850 4400 4432 5857
Size: 32 182 32 364 32 550 32 1425
Cuts at: 5857 5889 5931 5963 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 630 550 512 430 364 182
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2572 4060 4560 4806 669
Size: 988 281 634 1488 500 246 3079
Fragments arranged by size:
3079 1488 988 634 500 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7216
HpaI GTT'AAC
Cuts at: 4806 4806
Size: 7216
KpnI G_GTAC'C
Cuts at: 4541 4541
Size: 7216
MfeI C'AATT_G
Cuts at: 4815 4815
Size: 7216
MluI A'CGCG_T
Cuts at: 7092 7092
Size: 7216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6330 6514 1305
Size: 5025 184 2007
Fragments arranged by size:
5025 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3572 10
Size: 54 1425 343 1740 3654
Fragments arranged by size:
3654 1740 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3578 3779 3929 4265 5174 5533 5692
Size: 648 2412 201 150 336 909 359 159
Cuts at: 5692 518
Size: 2042
Fragments arranged by size:
2412 2042 909 648 359 336 201 159
150
MspA1I CmG'CkG
Cuts at: 1606 2782 3010 3023 3064 3253 3669 3779 4632
Size: 1176 228 13 41 189 416 110 853
Cuts at: 4632 5309 6457 6702 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1176 1148 853 677 416 245 228
189 110 41 13
NcoI C'CATG_G
Cuts at: 513 1065 2004 2695 3348 3618 513
Size: 552 939 691 653 270 4111
Fragments arranged by size:
4111 939 691 653 552 270
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7216
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7216
NotI GC'GGCC_GC
Cuts at: 4663 4663
Size: 7216
NspI r_CATG'y
Cuts at: 1132 1229 2926 3238 3655 4033 4574 6119 1132
Size: 97 1697 312 417 378 541 1545 2229
Fragments arranged by size:
2229 1697 1545 541 417 378 312 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7216
PciI A'CATG_T
Cuts at: 6115 6115
Size: 7216
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2316 3682 1108
Size: 1208 1366 4642
Fragments arranged by size:
4642 1366 1208
PfoI T'CCnGG_A
Cuts at: 2529 3072 3098 4116 2529
Size: 543 26 1018 5629
Fragments arranged by size:
5629 1018 543 26
PmeI GTTT'AAAC
Cuts at: 4520 4520
Size: 7216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5285 5317 6798 6830 5285
Size: 32 1481 32 5671
Fragments arranged by size:
5671 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7216
PsiI TTA'TAA
Cuts at: 4786 4786
Size: 7216
PspOMI G'GGCC_C
Cuts at: 2406 3125 2406
Size: 719 6497
PstI C_TGCA'G
Cuts at: 830 2342 2808 3270 3492 3555 3863 4101 4182
Size: 1512 466 462 222 63 308 238 81
Cuts at: 4182 4568 830
Size: 386 3478
Fragments arranged by size:
3478 1512 466 462 386 308 238 222
81 63
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7216
PvuII CAG'CTG
Cuts at: 2782 3023 2782
Size: 241 6975
SacI G_AGCT'C
Cuts at: 2985 2985
Size: 7216
SacII CC_GC'GG
Cuts at: 3670 3670
Size: 7216
SalI G'TCGA_C
Cuts at: 1936 4558 1936
Size: 2622 4594
SapI GCTCTTCn'nnn_
Cuts at: 2338 4124 2338
Size: 1786 5430
SbfI CC_TGCA'GG
Cuts at: 3270 3863 4568 3270
Size: 593 705 5918
Fragments arranged by size:
5918 705 593
ScaI AGT'ACT
Cuts at: 5464 7176 5464
Size: 1712 5504
SfcI C'TryA_G
Cuts at: 826 1045 2338 2804 3202 3266 3361 3488 3529
Size: 219 1293 466 398 64 95 127 41
Cuts at: 3529 3551 3859 4097 4178 4564 5699 6380 6571
Size: 22 308 238 81 386 1135 681 191
Cuts at: 6571 826
Size: 1471
Fragments arranged by size:
1471 1293 1135 681 466 398 386 308
238 219 191 127 95 81 64 41
22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7216
SgrAI Cr'CCGG_yG
Cuts at: 1700 2203 1700
Size: 503 6713
SmaI CCC'GGG
Cuts at: 1872 4290 4543 1872
Size: 2418 253 4545
Fragments arranged by size:
4545 2418 253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3599 4139 4404 5320 6221 6483
Size: 197 926 1656 540 265 916 901 262
Cuts at: 6483 820
Size: 1553
Fragments arranged by size:
1656 1553 926 916 901 540 265 262
197
SnaBI TAC'GTA
Cuts at: 493 5007 493
Size: 4514 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7216
SphI G_CATG'C
Cuts at: 1132 4574 1132
Size: 3442 3774
SspI AAT'ATT
Cuts at: 5 52 5140 5
Size: 47 5088 2081
Fragments arranged by size:
5088 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7216
StyI C'CwwG_G
Cuts at: 513 1065 2004 2695 3348 3618 3636 4655 513
Size: 552 939 691 653 270 18 1019 3074
Fragments arranged by size:
3074 1019 939 691 653 552 270 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4037 5243 5260 5413 5598 6043 325
Size: 1255 2457 1206 17 153 185 445 1498
Fragments arranged by size:
2457 1498 1255 1206 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2925 3330 3585
Size: 274 80 33 51 1443 948 405 255
Cuts at: 3585 3891 5462 7174 96
Size: 306 1571 1712 138
Fragments arranged by size:
1712 1571 1443 948 405 306 274 255
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3068 3100 7176 7208 3068
Size: 32 4076 32 3076
Fragments arranged by size:
4076 3076 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2230 1316
Size: 914 6302
XbaI T'CTAG_A
Cuts at: 4552 4552
Size: 7216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 4114 2845
Size: 1269 5947
XhoI C'TCGA_G
Cuts at: 1943 4404 1943
Size: 2461 4755
XmaI C'CCGG_G
Cuts at: 1870 4288 4541 1870
Size: 2418 253 4545
Fragments arranged by size:
4545 2418 253
XmnI GAAnn'nnTTC
Cuts at: 4133 4157 5345 4133
Size: 24 1188 6004
Fragments arranged by size:
6004 1188 24
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2680 5024 276
Size: 53 83 186 936 1146 2344 2468
Fragments arranged by size:
2468 2344 1146 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI