Restriction map for
pFN21AB9457
(Circular) (Six-base) MAPSORT of: pFN21AB9457.seq Check: 1884 from: 1 to: 7201
pFN21AB9457 7201 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2261 5011 278
Size: 53 83 186 936 725 2750 2468
Fragments arranged by size:
2750 2468 936 725 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2529 4445 4544 1937
Size: 592 1916 99 4594
Fragments arranged by size:
4594 1916 592 99
Acc65I G'GTAC_C
Cuts at: 4522 4522
Size: 7201
AclI AA'CG_TT
Cuts at: 1563 5328 5701 1563
Size: 3765 373 3063
Fragments arranged by size:
3765 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2291 2643 5264 6648 6916 1632
Size: 659 352 2621 1384 268 1917
Fragments arranged by size:
2621 1917 1384 659 352 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7004
AflIII A'CryG_T
Cuts at: 1203 3244 3431 3716 6100 7077 1203
Size: 2041 187 285 2384 977 1327
Fragments arranged by size:
2384 2041 1327 977 285 187
AgeI A'CCGG_T
Cuts at: 6032 6032
Size: 7201
AhdI GACnn_n'nnGTC
Cuts at: 2552 5930 2552
Size: 3378 3823
AleI CACnn'nnGTG
Cuts at: 1166 3534 1166
Size: 2368 4833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3552 3908 1184
Size: 2368 356 4477
Fragments arranged by size:
4477 2368 356
AlwNI CAG_nnn'CTG
Cuts at: 2054 4944 6516 2054
Size: 2890 1572 2739
Fragments arranged by size:
2890 2739 1572
ApaI G_GGCC'C
Cuts at: 2843 2843
Size: 7201
ApaLI G'TGCA_C
Cuts at: 5258 6414 5258
Size: 1156 6045
ApoI r'AATT_y
Cuts at: 1493 2039 2060 2083 3309 4510 4736 4888 6997
Size: 546 21 23 1226 1201 226 152 2109
Cuts at: 6997 1493
Size: 1697
Fragments arranged by size:
2109 1697 1226 1201 546 226 152 23
21
AseI AT'TA_AT
Cuts at: 160 1032 5755 160
Size: 872 4723 1606
Fragments arranged by size:
4723 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
AvaI C'yCGr_G
Cuts at: 1870 1943 2991 3615 4526 1870
Size: 73 1048 624 911 4545
Fragments arranged by size:
4545 1048 911 624 73
BamHI G'GATC_C
Cuts at: 2965 4531 4898 2965
Size: 1566 367 5268
Fragments arranged by size:
5268 1566 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2014 3216 4522 5977 618
Size: 325 217 627 227 1202 1306 1455 1842
Fragments arranged by size:
1842 1455 1306 1202 627 325 227 217
BanII G_rGCy'C
Cuts at: 1391 2414 2843 4520 1391
Size: 1023 429 1677 4072
Fragments arranged by size:
4072 1677 1023 429
BbeI G_GCGC'C
Cuts at: 2018 2018
Size: 7201
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2913 928
Size: 971 1014 5216
Fragments arranged by size:
5216 1014 971
BbvCI CC'TCA_GC
Cuts at: 3738 3738
Size: 7201
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3443 3477 5392 5426 1903
Size: 34 1506 34 1915 34 3678
Fragments arranged by size:
3678 1915 1506 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5093 6303 5093
Size: 1210 5991
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7201
BfrBI ATG'CAT
Cuts at: 3849 3849
Size: 7201
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3792 5812 136
Size: 107 122 71 631 605 2120 2020 1525
Fragments arranged by size:
2120 2020 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7196 7196
Size: 7201
BlpI GC'TnA_GC
Cuts at: 2379 3540 4618 2379
Size: 1161 1078 4962
Fragments arranged by size:
4962 1161 1078
Bme1580I G_kGCm'C
Cuts at: 1790 2843 3221 5262 6418 1790
Size: 1053 378 2041 1156 2573
Fragments arranged by size:
2573 2041 1156 1053 378
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3107 4447 4932
Size: 416 53 83 399 24 1690 1340 485
Cuts at: 4932 5890 442
Size: 958 1753
Fragments arranged by size:
1753 1690 1340 958 485 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7201
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2404 2686 2404
Size: 282 6919
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3165 4260 5861 1239
Size: 1926 1095 1601 2579
Fragments arranged by size:
2579 1926 1601 1095
Bpu10I CC'TnA_GC
Cuts at: 3738 3738
Size: 7201
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2955 3174 5326 6191 6489 2955
Size: 219 2152 865 298 3667
Fragments arranged by size:
3667 2152 865 298 219
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3183 5864 882
Size: 617 1684 2681 2219
Fragments arranged by size:
2681 2219 1684 617
BsaAI yAC'GTr
Cuts at: 493 1204 4992 493
Size: 711 3788 2702
Fragments arranged by size:
3788 2702 711
BsaBI GATnn'nnATC
Cuts at: 7195 7195
Size: 7201
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2258 4442 5008
Size: 53 83 186 936 482 243 2184 566
Cuts at: 5008 5390 6025 275
Size: 382 635 1451
Fragments arranged by size:
2184 1451 936 635 566 482 382 243
186 83 53
BsaWI w'CCGG_w
Cuts at: 2602 5634 6032 6306 6453 6864 2602
Size: 3032 398 274 147 411 2939
Fragments arranged by size:
3032 2939 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4118 4148 1178
Size: 30 165 30 2715 30 4231
Fragments arranged by size:
4231 2715 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3792 4385 1186
Size: 2606 593 4002
Fragments arranged by size:
4002 2606 593
BseYI C'CCAG_C
Cuts at: 3768 6404 6900 3768
Size: 2636 496 4069
Fragments arranged by size:
4069 2636 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2505 3225 4470 1163
Size: 573 769 720 1245 3894
Fragments arranged by size:
3894 1245 769 720 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4651 5412 6440 1540
Size: 275 186 2650 761 1028 2301
Fragments arranged by size:
2650 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2414 4131 5262 5347 6418 2414
Size: 1717 1131 85 1071 3197
Fragments arranged by size:
3197 1717 1131 1071 85
BsmI GAATG_Cn'
Cuts at: 4712 4805 4712
Size: 93 7108
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2414 2843 3221 4131 4520 5262 5347
Size: 399 624 429 378 910 389 742 85
Cuts at: 5347 6418 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 910 742 624 429 399 389
378 85
BspEI T'CCGG_A
Cuts at: 6864 6864
Size: 7201
BspHI T'CATG_A
Cuts at: 5090 6820 5090
Size: 1730 5471
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2170 4253 4325 4492 4556 844
Size: 1326 2083 72 167 64 3489
Fragments arranged by size:
3489 2083 1326 167 72 64
BsrBI CCG'CTC
Cuts at: 1394 1823 5088 1394
Size: 429 3265 3507
Fragments arranged by size:
3507 3265 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3758 5696 5870 65
Size: 1183 2510 1938 174 1396
Fragments arranged by size:
2510 1938 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5845 6032 1700
Size: 110 4035 187 2869
Fragments arranged by size:
4035 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7201
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7201
BssSI C'ACGA_G
Cuts at: 5261 6273 5261
Size: 1012 6189
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3084 794
Size: 2290 4911
BstBI TT'CG_AA
Cuts at: 6005 6005
Size: 7201
BstEII G'GTnAC_C
Cuts at: 1184 3707 1184
Size: 2523 4678
BstXI CCAn_nnnn'nTGG
Cuts at: 2635 2635
Size: 7201
BstYI r'GATC_y
Cuts at: 1972 2488 2965 4531 4898 5283 5300 6752 6838
Size: 516 477 1566 367 385 17 1452 86
Cuts at: 6838 6850 7196 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1566 1452 516 477 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2530 2530
Size: 7201
Bsu36I CC'TnA_GG
Cuts at: 2070 2070
Size: 7201
BtgI C'CryG_G
Cuts at: 513 1065 2004 2299 3550 3758 513
Size: 552 939 295 1251 208 3956
Fragments arranged by size:
3956 1251 939 552 295 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6462
Fragments arranged by size:
6462 683 56
BtsI GCAGTG_nn'
Cuts at: 4717 5511 5531 4717
Size: 794 20 6387
Fragments arranged by size:
6387 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7201
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7166
DraI TTT'AAA
Cuts at: 2038 3678 4505 2038
Size: 1640 827 4734
Fragments arranged by size:
4734 1640 827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6208 809
Size: 507 4892 1802
Fragments arranged by size:
4892 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3199 3352 3793
Size: 54 1425 50 275 18 1369 153 441
Cuts at: 3793 4648 5537 7166 8
Size: 855 889 1629 43
Fragments arranged by size:
1629 1425 1369 889 855 441 275 153
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4648 1537
Size: 275 2836 4090
Fragments arranged by size:
4090 2836 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2662 5131 1353
Size: 1309 2469 3423
Fragments arranged by size:
3423 2469 1309
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5788 6162 6308 387
Size: 1064 138 435 3764 374 146 1280
Fragments arranged by size:
3764 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2412 2412
Size: 7201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2643 3165 4260 5264 5861 6648
Size: 393 659 352 522 1095 1004 597 787
Cuts at: 6648 6916 1239
Size: 268 1524
Fragments arranged by size:
1524 1095 1004 787 659 597 522 393
352 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2025 2025
Size: 7201
EcoO109I rG'GnC_Cy
Cuts at: 2182 2840 3395 4296 2182
Size: 658 555 901 5087
Fragments arranged by size:
5087 901 658 555
EcoRI G'AATT_C
Cuts at: 2060 4510 4888 2060
Size: 2450 378 4373
Fragments arranged by size:
4373 2450 378
FspI TGC'GCA
Cuts at: 5707 5707
Size: 7201
HaeII r_GCGC'y
Cuts at: 1678 2018 6348 1678
Size: 340 4330 2531
Fragments arranged by size:
4330 2531 340
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2183 2215 2538 2570 2950
Size: 32 135 32 611 32 323 32 380
Cuts at: 2950 2982 4258 4290 5842 5874 5916 5948 1373
Size: 32 1276 32 1552 32 42 32 2626
Fragments arranged by size:
2626 1552 1276 611 380 323 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2935 4545 4791 669
Size: 988 281 997 1610 246 3079
Fragments arranged by size:
3079 1610 997 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7201
HpaI GTT'AAC
Cuts at: 4791 4791
Size: 7201
KasI G'GCGC_C
Cuts at: 2014 2014
Size: 7201
KpnI G_GTAC'C
Cuts at: 4526 4526
Size: 7201
MfeI C'AATT_G
Cuts at: 3812 4800 3812
Size: 988 6213
MluI A'CGCG_T
Cuts at: 7077 7077
Size: 7201
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3191 3818 6315 6499 1305
Size: 1886 627 2497 184 2007
Fragments arranged by size:
2497 2007 1886 627 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3201 3354 3795 10
Size: 54 1425 343 1369 153 441 3416
Fragments arranged by size:
3416 1425 1369 441 343 153 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3321 3436 3534 3924 4051 4179 4254
Size: 648 2155 115 98 390 127 128 75
Cuts at: 4254 4335 4492 5159 5518 5677 518
Size: 81 157 667 359 159 2042
Fragments arranged by size:
2155 2042 667 648 390 359 159 157
128 127 115 98 81 75
MspA1I CmG'CkG
Cuts at: 1606 2399 3672 3772 4617 5294 6442 6687 1606
Size: 793 1273 100 845 677 1148 245 2120
Fragments arranged by size:
2120 1273 1148 845 793 677 245 100
NarI GG'CG_CC
Cuts at: 2015 2015
Size: 7201
NcoI C'CATG_G
Cuts at: 513 1065 2004 2299 3550 513
Size: 552 939 295 1251 4164
Fragments arranged by size:
4164 1251 939 552 295
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7201
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7201
NotI GC'GGCC_GC
Cuts at: 4648 4648
Size: 7201
NsiI A_TGCA'T
Cuts at: 3851 3851
Size: 7201
NspI r_CATG'y
Cuts at: 1132 1229 3248 3435 3720 3849 4218 4497 4559
Size: 97 2019 187 285 129 369 279 62
Cuts at: 4559 6104 1132
Size: 1545 2229
Fragments arranged by size:
2229 2019 1545 369 285 279 187 129
97 62
PasI CC'CwG_GG
Cuts at: 1348 2493 2745 1348
Size: 1145 252 5804
Fragments arranged by size:
5804 1145 252
PciI A'CATG_T
Cuts at: 3244 3431 3716 6100 3244
Size: 187 285 2384 4345
Fragments arranged by size:
4345 2384 285 187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7201
PfoI T'CCnGG_A
Cuts at: 2924 3391 2924
Size: 467 6734
PmeI GTTT'AAAC
Cuts at: 4505 4505
Size: 7201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5270 5302 6783 6815 5270
Size: 32 1481 32 5656
Fragments arranged by size:
5656 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2182 3395 2182
Size: 1213 5988
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7201
PsiI TTA'TAA
Cuts at: 4771 4771
Size: 7201
PspOMI G'GGCC_C
Cuts at: 2839 2839
Size: 7201
PstI C_TGCA'G
Cuts at: 830 3989 4553 830
Size: 3159 564 3478
Fragments arranged by size:
3478 3159 564
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7201
PvuII CAG'CTG
Cuts at: 2399 3672 3772 2399
Size: 1273 100 5828
Fragments arranged by size:
5828 1273 100
SacI G_AGCT'C
Cuts at: 2414 2414
Size: 7201
SalI G'TCGA_C
Cuts at: 1936 4543 1936
Size: 2607 4594
SbfI CC_TGCA'GG
Cuts at: 4553 4553
Size: 7201
ScaI AGT'ACT
Cuts at: 5449 7161 5449
Size: 1712 5489
SexAI A'CCwGG_T
Cuts at: 3588 3588
Size: 7201
SfcI C'TryA_G
Cuts at: 826 1045 3985 4549 5684 6365 6556 826
Size: 219 2940 564 1135 681 191 1471
Fragments arranged by size:
2940 1471 1135 681 564 219 191
SfoI GGC'GCC
Cuts at: 2016 2016
Size: 7201
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7201
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7201
SmaI CCC'GGG
Cuts at: 1872 4528 1872
Size: 2656 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2970 3189 5305 6206 6468 820
Size: 197 926 1027 219 2116 901 262 1553
Fragments arranged by size:
2116 1553 1027 926 901 262 219 197
SnaBI TAC'GTA
Cuts at: 493 4992 493
Size: 4499 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7201
SphI G_CATG'C
Cuts at: 1132 4559 1132
Size: 3427 3774
SspI AAT'ATT
Cuts at: 5 52 3511 5125 5
Size: 47 3459 1614 2081
Fragments arranged by size:
3459 2081 1614 47
StuI AGG'CCT
Cuts at: 1517 2740 1517
Size: 1223 5978
StyI C'CwwG_G
Cuts at: 513 1065 2004 2299 2318 3550 4640 513
Size: 552 939 295 19 1232 1090 3074
Fragments arranged by size:
3074 1232 1090 939 552 295 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2471 5228 5245 5398 5583 6028 325
Size: 1255 891 2757 17 153 185 445 1498
Fragments arranged by size:
2757 1498 1255 891 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5447 7159 96
Size: 274 80 33 51 1443 3470 1712 138
Fragments arranged by size:
3470 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 7161 7193 4118
Size: 32 3011 32 4126
Fragments arranged by size:
4126 3011 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7201
XbaI T'CTAG_A
Cuts at: 2672 4537 2672
Size: 1865 5336
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7201
XmaI C'CCGG_G
Cuts at: 1870 4526 1870
Size: 2656 4545
XmnI GAAnn'nnTTC
Cuts at: 2064 3096 5330 2064
Size: 1032 2234 3935
Fragments arranged by size:
3935 2234 1032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2259 5009 276
Size: 53 83 186 936 725 2750 2468
Fragments arranged by size:
2750 2468 936 725 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BsiWI BsmBI
DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacII SanDI SapI
SfiI SrfI SwaI XcmI