Restriction map for
pFN21AB9456
(Circular) (Six-base) MAPSORT of: pFN21AB9456.seq Check: 2766 from: 1 to: 8155
pFN21AB9456 8155 bp
With 182 enzymes: SgfI *
April 15, 2010 09:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5965 278
Size: 53 83 186 936 4429 2468
Fragments arranged by size:
4429 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2577 2590 2964 5498 1937
Size: 640 13 374 2534 4594
Fragments arranged by size:
4594 2534 640 374 13
Acc65I G'GTAC_C
Cuts at: 2561 2828 5476 2561
Size: 267 2648 5240
Fragments arranged by size:
5240 2648 267
AclI AA'CG_TT
Cuts at: 1563 6282 6655 1563
Size: 4719 373 3063
Fragments arranged by size:
4719 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2022 2544 2642 3372 3743 6218 7602 7870
Size: 390 522 98 730 371 2475 1384 268
Cuts at: 7870 1632
Size: 1917
Fragments arranged by size:
2475 1917 1384 730 522 390 371 268
98
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7958
AflIII A'CryG_T
Cuts at: 1203 3743 4312 7054 8031 1203
Size: 2540 569 2742 977 1327
Fragments arranged by size:
2742 2540 1327 977 569
AgeI A'CCGG_T
Cuts at: 6986 6986
Size: 8155
AhdI GACnn_n'nnGTC
Cuts at: 6884 6884
Size: 8155
AleI CACnn'nnGTG
Cuts at: 1166 4376 4571 1166
Size: 3210 195 4750
Fragments arranged by size:
4750 3210 195
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8155
AlwNI CAG_nnn'CTG
Cuts at: 5215 5898 7470 5215
Size: 683 1572 5900
Fragments arranged by size:
5900 1572 683
ApaI G_GGCC'C
Cuts at: 2789 4051 2789
Size: 1262 6893
ApaLI G'TGCA_C
Cuts at: 3774 5138 6212 7368 3774
Size: 1364 1074 1156 4561
Fragments arranged by size:
4561 1364 1156 1074
ApoI r'AATT_y
Cuts at: 1493 2398 5464 5690 5842 7951 1493
Size: 905 3066 226 152 2109 1697
Fragments arranged by size:
3066 2109 1697 905 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6709 160
Size: 872 5677 1606
Fragments arranged by size:
5677 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8155
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 2145 2775 2895 3238 5175 5480
Size: 73 184 18 630 120 343 1937 305
Cuts at: 5480 1870
Size: 4545
Fragments arranged by size:
4545 1937 630 343 305 184 120 73
18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4846 4879 4846
Size: 33 8122
BamHI G'GATC_C
Cuts at: 2230 5485 5852 2230
Size: 3255 367 4533
Fragments arranged by size:
4533 3255 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2561 2743 2828 2861 3689
Size: 325 217 627 774 182 85 33 828
Cuts at: 3689 5476 6931 618
Size: 1787 1455 1842
Fragments arranged by size:
1842 1787 1455 828 774 627 325 217
182 85 33
BanII G_rGCy'C
Cuts at: 1391 2759 2789 4051 4385 5474 1391
Size: 1368 30 1262 334 1089 4072
Fragments arranged by size:
4072 1368 1262 1089 334 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2436 3090 4311 4631 4860 928
Size: 971 537 654 1221 320 229 4223
Fragments arranged by size:
4223 1221 971 654 537 320 229
BbvCI CC'TCA_GC
Cuts at: 2108 2108
Size: 8155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6346 6380 1903
Size: 34 50 34 4325 34 3678
Fragments arranged by size:
4325 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4202 4874 6047 7257 4202
Size: 672 1173 1210 5100
Fragments arranged by size:
5100 1210 1173 672
BclI T'GATC_A
Cuts at: 1548 5439 1548
Size: 3891 4264
BfrBI ATG'CAT
Cuts at: 2008 4939 5257 2008
Size: 2931 318 4906
Fragments arranged by size:
4906 2931 318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4551 6766 136
Size: 107 122 71 631 605 2879 2215 1525
Fragments arranged by size:
2879 2215 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3518 3526 8150 3518
Size: 8 4624 3523
Fragments arranged by size:
4624 3523 8
BlpI GC'TnA_GC
Cuts at: 4434 5572 4434
Size: 1138 7017
Bme1580I G_kGCm'C
Cuts at: 1790 2645 2782 2789 3778 4051 5142 6216 7372
Size: 855 137 7 989 273 1091 1074 1156
Cuts at: 7372 1790
Size: 2573
Fragments arranged by size:
2573 1156 1091 1074 989 855 273 137
7
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8155
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2232 2792 5886
Size: 416 53 83 399 24 815 560 3094
Cuts at: 5886 6844 442
Size: 958 1753
Fragments arranged by size:
3094 1753 958 815 560 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8155
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1998 2670 3105 3543 4039 4288 6815 1239
Size: 759 672 435 438 496 249 2527 2579
Fragments arranged by size:
2579 2527 759 672 496 438 435 249
Bpu10I CC'TnA_GC
Cuts at: 2108 2108
Size: 8155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2469 2912 6280 7145 7443 2469
Size: 443 3368 865 298 3181
Fragments arranged by size:
3368 3181 865 443 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4015 4373 6818 882
Size: 617 2516 358 2445 2219
Fragments arranged by size:
2516 2445 2219 617 358
BsaAI yAC'GTr
Cuts at: 493 1204 5946 493
Size: 711 4742 2702
Fragments arranged by size:
4742 2702 711
BsaBI GATnn'nnATC
Cuts at: 8149 8149
Size: 8155
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4597 5962 6344 6979
Size: 53 83 186 936 3064 1365 382 635
Cuts at: 6979 275
Size: 1451
Fragments arranged by size:
3064 1451 1365 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2101 2244 5230 6588 6986 7260 7407 7818 2101
Size: 143 2986 1358 398 274 147 411 2438
Fragments arranged by size:
2986 2438 1358 411 398 274 147 143
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7930
Fragments arranged by size:
7930 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2441 3099 4322 1186
Size: 1255 658 1223 5019
Fragments arranged by size:
5019 1255 1223 658
BseYI C'CCAG_C
Cuts at: 2750 3133 4703 7358 7854 2750
Size: 383 1570 2655 496 3051
Fragments arranged by size:
3051 2655 1570 496 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4233 4840 1163
Size: 573 2497 607 4478
Fragments arranged by size:
4478 2497 607 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5605 6366 7394 1540
Size: 275 186 3604 761 1028 2301
Fragments arranged by size:
3604 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3778 5142 6216 6301 7372 3778
Size: 1364 1074 85 1071 4561
Fragments arranged by size:
4561 1364 1074 1071 85
BsiWI C'GTAC_G
Cuts at: 3013 3013
Size: 8155
BsmI GAATG_Cn'
Cuts at: 3306 5666 5759 3306
Size: 2360 93 5702
Fragments arranged by size:
5702 2360 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2645 2759 2782 2789 3778 4051 4385
Size: 399 855 114 23 7 989 273 334
Cuts at: 4385 5142 5474 6216 6301 7372 1391
Size: 757 332 742 85 1071 2174
Fragments arranged by size:
2174 1071 989 855 757 742 399 334
332 273 114 85 23 7
BspEI T'CCGG_A
Cuts at: 2101 5230 7818 2101
Size: 3129 2588 2438
Fragments arranged by size:
3129 2588 2438
BspHI T'CATG_A
Cuts at: 6044 7774 6044
Size: 1730 6425
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5223 5510 844
Size: 4379 287 3489
Fragments arranged by size:
4379 3489 287
BsrBI CCG'CTC
Cuts at: 1394 1823 6042 1394
Size: 429 4219 3507
Fragments arranged by size:
4219 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2724 3610 3888 3909 4714 6650 6824
Size: 1183 1476 886 278 21 805 1936 174
Cuts at: 6824 65
Size: 1396
Fragments arranged by size:
1936 1476 1396 1183 886 805 278 174
21
BsrFI r'CCGG_y
Cuts at: 1700 1810 6799 6986 1700
Size: 110 4989 187 2869
Fragments arranged by size:
4989 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2693 2850 96
Size: 2597 157 5401
Fragments arranged by size:
5401 2597 157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8155
BssSI C'ACGA_G
Cuts at: 6215 7227 6215
Size: 1012 7143
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2222 794
Size: 1428 6727
BstBI TT'CG_AA
Cuts at: 4006 6959 4006
Size: 2953 5202
BstEII G'GTnAC_C
Cuts at: 1184 3137 1184
Size: 1953 6202
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2212 3711 4541 4711 2032
Size: 180 1499 830 170 5476
Fragments arranged by size:
5476 1499 830 180 170
BstYI r'GATC_y
Cuts at: 1972 2230 3518 3526 3922 4830 5485 5852 6237
Size: 258 1288 8 396 908 655 367 385
Cuts at: 6237 6254 7706 7792 7804 8150 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1288 908 655 396 385 367
346 258 86 17 12 8
Bsu36I CC'TnA_GG
Cuts at: 5349 5349
Size: 8155
BtgI C'CryG_G
Cuts at: 513 1065 3710 4122 4195 513
Size: 552 2645 412 73 4473
Fragments arranged by size:
4473 2645 552 412 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7416
Fragments arranged by size:
7416 683 56
BtsI GCAGTG_nn'
Cuts at: 4670 5271 5437 5671 6465 6485 4670
Size: 601 166 234 794 20 6340
Fragments arranged by size:
6340 794 601 234 166 20
ClaI AT'CG_AT
Cuts at: 1554 2070 1554
Size: 516 7639
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3571 3606 529
Size: 35 3007 35 5078
Fragments arranged by size:
5078 3007 35 35
DraI TTT'AAA
Cuts at: 5459 5459
Size: 8155
DraIII CAC_nnn'GTG
Cuts at: 2240 4807 2240
Size: 2567 5588
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4313 7162 809
Size: 507 2997 2849 1802
Fragments arranged by size:
2997 2849 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 3072 3701
Size: 54 1425 50 275 18 633 609 629
Cuts at: 3701 3835 4497 5602 6491 8120 8
Size: 134 662 1105 889 1629 43
Fragments arranged by size:
1629 1425 1105 889 662 633 629 609
275 134 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5602 1537
Size: 275 3790 4090
Fragments arranged by size:
4090 3790 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2499 5106 6085 1353
Size: 1146 2607 979 3423
Fragments arranged by size:
3423 2607 1146 979
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4931 5266 6742 7116 7262 387
Size: 1064 138 3342 335 1476 374 146 1280
Fragments arranged by size:
3342 1476 1280 1064 374 335 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2022 2544 2642 2670 3105 3372
Size: 393 366 24 522 98 28 435 267
Cuts at: 3372 3543 3743 4039 4288 6218 6815 7602 7870
Size: 171 200 296 249 1930 597 787 268
Cuts at: 7870 1239
Size: 1524
Fragments arranged by size:
1930 1524 787 597 522 435 393 366
296 268 267 249 200 171 98 28
24
EcoNI CCTnn'n_nnAGG
Cuts at: 3362 4457 3362
Size: 1095 7060
EcoO109I rG'GnC_Cy
Cuts at: 2275 4048 4247 4879 4995 5212 2275
Size: 1773 199 632 116 217 5218
Fragments arranged by size:
5218 1773 632 217 199 116
EcoRI G'AATT_C
Cuts at: 5464 5842 5464
Size: 378 7777
FspI TGC'GCA
Cuts at: 6661 6661
Size: 8155
HaeII r_GCGC'y
Cuts at: 1678 7302 1678
Size: 5624 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2096 2128 2889 2921 3158
Size: 32 135 32 524 32 761 32 237
Cuts at: 3158 3190 3360 3392 4060 4092 5054 5086 6796
Size: 32 170 32 668 32 962 32 1710
Cuts at: 6796 6828 6870 6902 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1710 962 761 668 524 237 170
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2703 3442 5290 5499 5745 669
Size: 988 281 765 739 1848 209 246 3079
Fragments arranged by size:
3079 1848 988 765 739 281 246 209
HindIII A'AGCT_T
Cuts at: 748 4422 748
Size: 3674 4481
HpaI GTT'AAC
Cuts at: 5290 5745 5290
Size: 455 7700
KpnI G_GTAC'C
Cuts at: 2565 2832 5480 2565
Size: 267 2648 5240
Fragments arranged by size:
5240 2648 267
MfeI C'AATT_G
Cuts at: 2670 5051 5754 2670
Size: 2381 703 5071
Fragments arranged by size:
5071 2381 703
MluI A'CGCG_T
Cuts at: 8031 8031
Size: 8155
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7269 7453 1305
Size: 5964 184 2007
Fragments arranged by size:
5964 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 3074 3703 3837 10
Size: 54 1425 343 633 609 629 134 4328
Fragments arranged by size:
4328 1425 633 629 609 343 134 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2030 2916 3429 3709 4064 4376 4571
Size: 648 864 886 513 280 355 312 195
Cuts at: 4571 4739 4944 4979 6113 6472 6631 518
Size: 168 205 35 1134 359 159 2042
Fragments arranged by size:
2042 1134 886 864 648 513 359 355
312 280 205 195 168 159 35
MspA1I CmG'CkG
Cuts at: 1606 2648 2750 4489 5571 6248 7396 7641 1606
Size: 1042 102 1739 1082 677 1148 245 2120
Fragments arranged by size:
2120 1739 1148 1082 1042 677 245 102
NcoI C'CATG_G
Cuts at: 513 1065 3710 4122 513
Size: 552 2645 412 4546
Fragments arranged by size:
4546 2645 552 412
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8155
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8155
NotI GC'GGCC_GC
Cuts at: 5602 5602
Size: 8155
NsiI A_TGCA'T
Cuts at: 2010 4941 5259 2010
Size: 2931 318 4906
Fragments arranged by size:
4906 2931 318
NspI r_CATG'y
Cuts at: 1132 1229 2959 3133 3627 3747 3987 4316 5017
Size: 97 1730 174 494 120 240 329 701
Cuts at: 5017 5227 5513 7058 1132
Size: 210 286 1545 2229
Fragments arranged by size:
2229 1730 1545 701 494 329 286 240
210 174 120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8155
PciI A'CATG_T
Cuts at: 3743 4312 7054 3743
Size: 569 2742 4844
Fragments arranged by size:
4844 2742 569
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2795 4492 1108
Size: 1687 1697 4771
Fragments arranged by size:
4771 1697 1687
PfoI T'CCnGG_A
Cuts at: 3310 5208 3310
Size: 1898 6257
PmeI GTTT'AAAC
Cuts at: 5459 5459
Size: 8155
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6224 6256 7737 7769 6224
Size: 32 1481 32 6610
Fragments arranged by size:
6610 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2275 4247 4879 4995 5212 2275
Size: 1972 632 116 217 5218
Fragments arranged by size:
5218 1972 632 217 116
PshAI GACnn'nnGTC
Cuts at: 1613 2097 2280 1613
Size: 484 183 7488
Fragments arranged by size:
7488 484 183
PsiI TTA'TAA
Cuts at: 5725 5725
Size: 8155
PspOMI G'GGCC_C
Cuts at: 2785 4047 2785
Size: 1262 6893
PspXI vC'TCGA_Gb
Cuts at: 3238 3238
Size: 8155
PstI C_TGCA'G
Cuts at: 830 5507 830
Size: 4677 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8155
PvuII CAG'CTG
Cuts at: 2648 2750 4489 2648
Size: 102 1739 6314
Fragments arranged by size:
6314 1739 102
SalI G'TCGA_C
Cuts at: 1936 5497 1936
Size: 3561 4594
SbfI CC_TGCA'GG
Cuts at: 5507 5507
Size: 8155
ScaI AGT'ACT
Cuts at: 6403 8115 6403
Size: 1712 6443
SfcI C'TryA_G
Cuts at: 826 1045 5503 6638 7319 7510 826
Size: 219 4458 1135 681 191 1471
Fragments arranged by size:
4458 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8155
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8155
SmaI CCC'GGG
Cuts at: 1872 2147 2777 5177 5482 1872
Size: 275 630 2400 305 4545
Fragments arranged by size:
4545 2400 630 305 275
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2484 2927 3238 6259 7160 7422
Size: 197 926 541 443 311 3021 901 262
Cuts at: 7422 820
Size: 1553
Fragments arranged by size:
3021 1553 926 901 541 443 311 262
197
SnaBI TAC'GTA
Cuts at: 493 5946 493
Size: 5453 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8155
SphI G_CATG'C
Cuts at: 1132 3133 5513 1132
Size: 2001 2380 3774
Fragments arranged by size:
3774 2380 2001
SspI AAT'ATT
Cuts at: 5 52 2387 2599 6079 5
Size: 47 2335 212 3480 2081
Fragments arranged by size:
3480 2335 2081 212 47
StuI AGG'CCT
Cuts at: 1517 4273 1517
Size: 2756 5399
StyI C'CwwG_G
Cuts at: 513 1065 2857 3183 3363 3710 3801 4122 4162
Size: 552 1792 326 180 347 91 321 40
Cuts at: 4162 4546 5594 513
Size: 384 1048 3074
Fragments arranged by size:
3074 1792 1048 552 384 347 326 321
180 91 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2251 2449 2561 2623 3703 4027 5079
Size: 1255 671 198 112 62 1080 324 1052
Cuts at: 5079 6182 6199 6352 6537 6982 325
Size: 1103 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1103 1080 1052 671 445 324
198 185 153 112 62 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2693 2850 4149
Size: 274 80 33 51 1443 716 157 1299
Cuts at: 4149 5163 6401 8113 96
Size: 1014 1238 1712 138
Fragments arranged by size:
1712 1443 1299 1238 1014 716 274 157
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8115 8147 8115
Size: 32 8123
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8155
XbaI T'CTAG_A
Cuts at: 5491 5491
Size: 8155
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2209 2737 3216 3308 4120 2029
Size: 180 528 479 92 812 6064
Fragments arranged by size:
6064 812 528 479 180 92
XhoI C'TCGA_G
Cuts at: 1943 3238 1943
Size: 1295 6860
XmaI C'CCGG_G
Cuts at: 1870 2145 2775 5175 5480 1870
Size: 275 630 2400 305 4545
Fragments arranged by size:
4545 2400 630 305 275
XmnI GAAnn'nnTTC
Cuts at: 2874 3432 6284 2874
Size: 558 2852 4745
Fragments arranged by size:
4745 2852 558
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5963 276
Size: 53 83 186 936 4429 2468
Fragments arranged by size:
4429 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BplI BsmBI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI