Restriction map for
pFN21AB9453
(Circular) (Six-base) MAPSORT of: pFN21AB9453.seq Check: 2156 from: 1 to: 6622
pFN21AB9453 6622 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2389 2389
Size: 6622
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4432 278
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3083 3965 1937
Size: 1146 882 4594
Fragments arranged by size:
4594 1146 882
Acc65I G'GTAC_C
Cuts at: 3943 3943
Size: 6622
AclI AA'CG_TT
Cuts at: 1563 3921 4749 5122 1563
Size: 2358 828 373 3063
Fragments arranged by size:
3063 2358 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2516 2702 2856 3108 4685 6069 6337 1632
Size: 884 186 154 252 1577 1384 268 1917
Fragments arranged by size:
1917 1577 1384 884 268 252 186 154
AfeI AGC'GCT
Cuts at: 3610 3610
Size: 6622
AflII C'TTAA_G
Cuts at: 820 1017 2788 820
Size: 197 1771 4654
Fragments arranged by size:
4654 1771 197
AflIII A'CryG_T
Cuts at: 1203 2405 2407 5521 6498 1203
Size: 1202 2 3114 977 1327
Fragments arranged by size:
3114 1327 1202 977 2
AgeI A'CCGG_T
Cuts at: 5453 5453
Size: 6622
AhdI GACnn_n'nnGTC
Cuts at: 5351 5351
Size: 6622
AleI CACnn'nnGTG
Cuts at: 1166 2834 3701 1166
Size: 1668 867 4087
Fragments arranged by size:
4087 1668 867
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3669 1184
Size: 2485 4137
AlwNI CAG_nnn'CTG
Cuts at: 2987 3355 4365 5937 2987
Size: 368 1010 1572 3672
Fragments arranged by size:
3672 1572 1010 368
ApaI G_GGCC'C
Cuts at: 2481 3174 3311 3748 2481
Size: 693 137 437 5355
Fragments arranged by size:
5355 693 437 137
ApaLI G'TGCA_C
Cuts at: 4679 5835 4679
Size: 1156 5466
ApoI r'AATT_y
Cuts at: 1493 2882 3112 3931 4157 4309 6418 1493
Size: 1389 230 819 226 152 2109 1697
Fragments arranged by size:
2109 1697 1389 819 230 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5176 160
Size: 872 4144 1606
Fragments arranged by size:
4144 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2292 3716 3947 1870
Size: 73 196 153 1424 231 4545
Fragments arranged by size:
4545 1424 231 196 153 73
BamHI G'GATC_C
Cuts at: 3952 4319 3952
Size: 367 6255
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3324 3703 3943 5398 618
Size: 325 217 627 1537 379 240 1455 1842
Fragments arranged by size:
1842 1537 1455 627 379 325 240 217
BanII G_rGCy'C
Cuts at: 1391 2259 2481 2807 2996 3174 3311 3377 3676
Size: 868 222 326 189 178 137 66 299
Cuts at: 3676 3748 3941 1391
Size: 72 193 4072
Fragments arranged by size:
4072 868 326 299 222 193 189 178
137 72 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2099 2187 2778 928
Size: 971 200 88 591 4772
Fragments arranged by size:
4772 971 591 200 88
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4813 4847 1903
Size: 34 2876 34 3678
Fragments arranged by size:
3678 2876 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4514 5724 4514
Size: 1210 5412
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6622
BfrBI ATG'CAT
Cuts at: 3151 3151
Size: 6622
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5233 136
Size: 107 122 71 631 605 3561 1525
Fragments arranged by size:
3561 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6617 6617
Size: 6622
BlpI GC'TnA_GC
Cuts at: 4039 4039
Size: 6622
Bme1580I G_kGCm'C
Cuts at: 1790 2481 3174 3311 3327 3708 3748 4683 5839
Size: 691 693 137 16 381 40 935 1156
Cuts at: 5839 1790
Size: 2573
Fragments arranged by size:
2573 1156 935 693 691 381 137 40
16
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6622
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2972 3453 4353
Size: 416 53 83 399 24 1555 481 900
Cuts at: 4353 5311 442
Size: 958 1753
Fragments arranged by size:
1753 1555 958 900 481 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2382 2685 3651 3876 5282 1239
Size: 1143 303 966 225 1406 2579
Fragments arranged by size:
2579 1406 1143 966 303 225
Bpu10I CC'TnA_GC
Cuts at: 2253 2913 2253
Size: 660 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4747 5612 5910 4747
Size: 865 298 5459
Fragments arranged by size:
5459 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2202 2804 5285 882
Size: 617 703 602 2481 2219
Fragments arranged by size:
2481 2219 703 617 602
BsaAI yAC'GTr
Cuts at: 493 1204 2408 4413 493
Size: 711 1204 2005 2702
Fragments arranged by size:
2702 2005 1204 711
BsaBI GATnn'nnATC
Cuts at: 2531 6616 2531
Size: 4085 2537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4429 4811 5446 275
Size: 53 83 186 936 2896 382 635 1451
Fragments arranged by size:
2896 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2012 2365 5055 5453 5727 5874 6285 2012
Size: 353 2690 398 274 147 411 2349
Fragments arranged by size:
2690 2349 411 398 353 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6397
Fragments arranged by size:
6397 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2339 3327 3798 3801 1186
Size: 888 265 988 471 3 4007
Fragments arranged by size:
4007 988 888 471 265 3
BseYI C'CCAG_C
Cuts at: 2980 3209 3542 5825 6321 2980
Size: 229 333 2283 496 3281
Fragments arranged by size:
3281 2283 496 333 229
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6049
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2013 4072 4833 5861 1540
Size: 275 186 12 2059 761 1028 2301
Fragments arranged by size:
2301 2059 1028 761 275 186 12
BsiHKAI G_wGCw'C
Cuts at: 2807 4683 4768 5839 2807
Size: 1876 85 1071 3590
Fragments arranged by size:
3590 1876 1071 85
BsmI GAATG_Cn'
Cuts at: 2115 4133 4226 2115
Size: 2018 93 4511
Fragments arranged by size:
4511 2018 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3078 3078
Size: 6622
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2481 2807 2996 3174 3311 3327
Size: 399 469 222 326 189 178 137 16
Cuts at: 3327 3377 3676 3708 3748 3941 4683 4768 5839
Size: 50 299 32 40 193 742 85 1071
Cuts at: 5839 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 469 399 326 299 222
193 189 178 137 85 50 40 32
16
BspEI T'CCGG_A
Cuts at: 2365 6285 2365
Size: 3920 2702
BspHI T'CATG_A
Cuts at: 2424 4511 6241 2424
Size: 2087 1730 2805
Fragments arranged by size:
2805 2087 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2389 3977 844
Size: 1545 1588 3489
Fragments arranged by size:
3489 1588 1545
BsrBI CCG'CTC
Cuts at: 1394 1823 2329 4509 1394
Size: 429 506 2180 3507
Fragments arranged by size:
3507 2180 506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2524 2865 5117 5291 65
Size: 1183 1276 341 2252 174 1396
Fragments arranged by size:
2252 1396 1276 1183 341 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3782 5266 5453 1700
Size: 110 1972 1484 187 2869
Fragments arranged by size:
2869 1972 1484 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6622
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6622
BssSI C'ACGA_G
Cuts at: 3604 4682 5694 3604
Size: 1078 1012 4532
Fragments arranged by size:
4532 1078 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6622
BstBI TT'CG_AA
Cuts at: 5426 5426
Size: 6622
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6622
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2638 2894 3022 3273 3320 2032
Size: 606 256 128 251 47 5334
Fragments arranged by size:
5334 606 256 251 128 47
BstYI r'GATC_y
Cuts at: 1972 2784 3952 4319 4704 4721 6173 6259 6271
Size: 812 1168 367 385 17 1452 86 12
Cuts at: 6271 6617 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1168 812 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2232 2232
Size: 6622
BtgI C'CryG_G
Cuts at: 513 1065 2004 3142 3328 3500 3575 3632 3822
Size: 552 939 1138 186 172 75 57 190
Cuts at: 3822 513
Size: 3313
Fragments arranged by size:
3313 1138 939 552 190 186 172 75
57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5883
Fragments arranged by size:
5883 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 4138 4932 4952 2664
Size: 1474 794 20 4334
Fragments arranged by size:
4334 1474 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6587
DraI TTT'AAA
Cuts at: 3926 3926
Size: 6622
DraIII CAC_nnn'GTG
Cuts at: 2838 2838
Size: 6622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5629 809
Size: 507 4313 1802
Fragments arranged by size:
4313 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2581 2860 3024
Size: 54 1425 50 275 18 751 279 164
Cuts at: 3024 4069 4958 6587 8
Size: 1045 889 1629 43
Fragments arranged by size:
1629 1425 1045 889 751 279 275 164
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4069 1537
Size: 275 2257 4090
Fragments arranged by size:
4090 2257 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2051 2316 3498 4552 1353
Size: 698 265 1182 1054 3423
Fragments arranged by size:
3423 1182 1054 698 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3516 3528 3747 3769 3804 5209
Size: 1064 138 1927 12 219 22 35 1405
Cuts at: 5209 5583 5729 387
Size: 374 146 1280
Fragments arranged by size:
1927 1405 1280 1064 374 219 146 138
35 22 12
EcoICRI GAG'CTC
Cuts at: 2805 2805
Size: 6622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2382 2516 2685 2702 2856 3108 3651
Size: 393 750 134 169 17 154 252 543
Cuts at: 3651 3876 4685 5282 6069 6337 1239
Size: 225 809 597 787 268 1524
Fragments arranged by size:
1524 809 787 750 597 543 393 268
252 225 169 154 134 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2684 2684
Size: 6622
EcoO109I rG'GnC_Cy
Cuts at: 2241 2760 3307 3308 3744 3745 2241
Size: 519 547 1 436 1 5118
Fragments arranged by size:
5118 547 519 436 1 1
EcoRI G'AATT_C
Cuts at: 3112 3931 4309 3112
Size: 819 378 5425
Fragments arranged by size:
5425 819 378
FspI TGC'GCA
Cuts at: 2201 5128 2201
Size: 2927 3695
HaeII r_GCGC'y
Cuts at: 1678 3612 5769 1678
Size: 1934 2157 2531
Fragments arranged by size:
2531 2157 1934
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2225 2257 2891 2923 5263
Size: 32 135 32 653 32 634 32 2340
Cuts at: 5263 5295 5337 5369 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2340 653 634 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3966 4212 669
Size: 988 281 2028 246 3079
Fragments arranged by size:
3079 2028 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6622
HpaI GTT'AAC
Cuts at: 4212 4212
Size: 6622
KpnI G_GTAC'C
Cuts at: 3947 3947
Size: 6622
MfeI C'AATT_G
Cuts at: 4221 4221
Size: 6622
MluI A'CGCG_T
Cuts at: 6498 6498
Size: 6622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2013 5736 5920 1305
Size: 708 3723 184 2007
Fragments arranged by size:
3723 2007 708 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2583 2862 3026 10
Size: 54 1425 343 751 279 164 3606
Fragments arranged by size:
3606 1425 751 343 279 164 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2834 3156 3701 3848 3860 4580
Size: 648 1182 486 322 545 147 12 720
Cuts at: 4580 4939 5098 518
Size: 359 159 2042
Fragments arranged by size:
2042 1182 720 648 545 486 359 322
159 147 12
MspA1I CmG'CkG
Cuts at: 1606 2067 4038 4715 5863 6108 1606
Size: 461 1971 677 1148 245 2120
Fragments arranged by size:
2120 1971 1148 677 461 245
NaeI GCC'GGC
Cuts at: 3784 3784
Size: 6622
NcoI C'CATG_G
Cuts at: 513 1065 2004 3328 3500 3575 3632 3822 513
Size: 552 939 1324 172 75 57 190 3313
Fragments arranged by size:
3313 1324 939 552 190 172 75 57
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6622
NgoMIV G'CCGG_C
Cuts at: 3782 3782
Size: 6622
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6622
NotI GC'GGCC_GC
Cuts at: 4069 4069
Size: 6622
NsiI A_TGCA'T
Cuts at: 3153 3153
Size: 6622
NspI r_CATG'y
Cuts at: 1132 1229 3151 3874 3980 5525 1132
Size: 97 1922 723 106 1545 2229
Fragments arranged by size:
2229 1922 1545 723 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6622
PciI A'CATG_T
Cuts at: 5521 5521
Size: 6622
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3773 1108
Size: 2665 3957
PfoI T'CCnGG_A
Cuts at: 2104 3667 2104
Size: 1563 5059
PmeI GTTT'AAAC
Cuts at: 3926 3926
Size: 6622
PmlI CAC'GTG
Cuts at: 2408 2408
Size: 6622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4691 4723 6204 6236 4691
Size: 32 1481 32 5077
Fragments arranged by size:
5077 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2241 2760 2241
Size: 519 6103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6622
PsiI TTA'TAA
Cuts at: 4192 4192
Size: 6622
PspOMI G'GGCC_C
Cuts at: 2477 3170 3307 3744 2477
Size: 693 137 437 5355
Fragments arranged by size:
5355 693 437 137
PstI C_TGCA'G
Cuts at: 830 2175 2386 3974 830
Size: 1345 211 1588 3478
Fragments arranged by size:
3478 1588 1345 211
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6622
SacI G_AGCT'C
Cuts at: 2807 2807
Size: 6622
SalI G'TCGA_C
Cuts at: 1936 3964 1936
Size: 2028 4594
SanDI GG'GwC_CC
Cuts at: 2760 2760
Size: 6622
SbfI CC_TGCA'GG
Cuts at: 3974 3974
Size: 6622
ScaI AGT'ACT
Cuts at: 4870 6582 4870
Size: 1712 4910
SfcI C'TryA_G
Cuts at: 826 1045 2115 2171 2382 3970 5105 5786 5977
Size: 219 1070 56 211 1588 1135 681 191
Cuts at: 5977 826
Size: 1471
Fragments arranged by size:
1588 1471 1135 1070 681 219 211 191
56
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6622
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6622
SmaI CCC'GGG
Cuts at: 1872 3718 3949 1872
Size: 1846 231 4545
Fragments arranged by size:
4545 1846 231
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2139 2788 4726 5627 5889 820
Size: 197 926 196 649 1938 901 262 1553
Fragments arranged by size:
1938 1553 926 901 649 262 197 196
SnaBI TAC'GTA
Cuts at: 493 4413 493
Size: 3920 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6622
SphI G_CATG'C
Cuts at: 1132 3151 3874 3980 1132
Size: 2019 723 106 3774
Fragments arranged by size:
3774 2019 723 106
SspI AAT'ATT
Cuts at: 5 52 2942 4546 5
Size: 47 2890 1604 2081
Fragments arranged by size:
2890 2081 1604 47
StuI AGG'CCT
Cuts at: 1517 2893 1517
Size: 1376 5246
StyI C'CwwG_G
Cuts at: 513 1065 2004 3272 3328 3500 3575 3632 3822
Size: 552 939 1268 56 172 75 57 190
Cuts at: 3822 4061 513
Size: 239 3074
Fragments arranged by size:
3074 1268 939 552 239 190 172 75
57 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2954 3104 3216 4649 4666 4819 5004
Size: 1255 1374 150 112 1433 17 153 185
Cuts at: 5004 5449 325
Size: 445 1498
Fragments arranged by size:
1498 1433 1374 1255 445 185 153 150
112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4868 6580 96
Size: 274 80 33 51 1443 2891 1712 138
Fragments arranged by size:
2891 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6582 6614 6582
Size: 32 6590
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6622
XbaI T'CTAG_A
Cuts at: 3958 3958
Size: 6622
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3019 3149 3550 3853 3019
Size: 130 401 303 5788
Fragments arranged by size:
5788 401 303 130
XhoI C'TCGA_G
Cuts at: 1943 2139 1943
Size: 196 6426
XmaI C'CCGG_G
Cuts at: 1870 3716 3947 1870
Size: 1846 231 4545
Fragments arranged by size:
4545 1846 231
XmnI GAAnn'nnTTC
Cuts at: 4751 4751
Size: 6622
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4430 276
Size: 53 83 186 936 2896 2468
Fragments arranged by size:
2896 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
PacI PspXI PsrI PvuII RsrII SacII SapI SexAI
SfiI SfoI SrfI SwaI