Restriction map for
pFN21AB9452
(Circular) (Six-base) MAPSORT of: pFN21AB9452.seq Check: 1770 from: 1 to: 6262
pFN21AB9452 6262 bp
With 182 enzymes: SgfI *
March 28, 2012 13:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4072 278
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 3605 1937
Size: 1243 425 4594
Fragments arranged by size:
4594 1243 425
Acc65I G'GTAC_C
Cuts at: 3583 3583
Size: 6262
AclI AA'CG_TT
Cuts at: 1563 4389 4762 1563
Size: 2826 373 3063
Fragments arranged by size:
3063 2826 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2224 3122 4325 5709 5977 1632
Size: 592 898 1203 1384 268 1917
Fragments arranged by size:
1917 1384 1203 898 592 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6065
AflIII A'CryG_T
Cuts at: 1203 5161 6138 1203
Size: 3958 977 1327
Fragments arranged by size:
3958 1327 977
AgeI A'CCGG_T
Cuts at: 5093 5093
Size: 6262
AhdI GACnn_n'nnGTC
Cuts at: 3355 4991 3355
Size: 1636 4626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6262
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2273 1184
Size: 1089 5173
AlwNI CAG_nnn'CTG
Cuts at: 2494 2695 3112 4005 5577 2494
Size: 201 417 893 1572 3179
Fragments arranged by size:
3179 1572 893 417 201
ApaI G_GGCC'C
Cuts at: 3119 3119
Size: 6262
ApaLI G'TGCA_C
Cuts at: 4319 5475 4319
Size: 1156 5106
ApoI r'AATT_y
Cuts at: 1493 2369 3292 3571 3797 3949 6058 1493
Size: 876 923 279 226 152 2109 1697
Fragments arranged by size:
2109 1697 923 876 279 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4816 160
Size: 872 3784 1606
Fragments arranged by size:
3784 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
AvaI C'yCGr_G
Cuts at: 1870 1943 2092 2465 3587 1870
Size: 73 149 373 1122 4545
Fragments arranged by size:
4545 1122 373 149 73
BamHI G'GATC_C
Cuts at: 3592 3959 3592
Size: 367 5895
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2637 2680 3318 3583 5038
Size: 325 217 627 850 43 638 265 1455
Cuts at: 5038 618
Size: 1842
Fragments arranged by size:
1842 1455 850 638 627 325 265 217
43
BanII G_rGCy'C
Cuts at: 1391 2635 3119 3345 3581 1391
Size: 1244 484 226 236 4072
Fragments arranged by size:
4072 1244 484 236 226
BbeI G_GCGC'C
Cuts at: 2641 2641
Size: 6262
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3074 3468 928
Size: 971 1175 394 3722
Fragments arranged by size:
3722 1175 971 394
BbvCI CC'TCA_GC
Cuts at: 2057 2765 2057
Size: 708 5554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4453 4487 1903
Size: 34 2516 34 3678
Fragments arranged by size:
3678 2516 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4154 5364 4154
Size: 1210 5052
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6262
BfrBI ATG'CAT
Cuts at: 3094 3094
Size: 6262
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4873 136
Size: 107 122 71 631 605 337 2864 1525
Fragments arranged by size:
2864 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6257 6257
Size: 6262
BlpI GC'TnA_GC
Cuts at: 3412 3679 3412
Size: 267 5995
Bme1580I G_kGCm'C
Cuts at: 1790 2306 2685 3119 4323 5479 1790
Size: 516 379 434 1204 1156 2573
Fragments arranged by size:
2573 1204 1156 516 434 379
BmgBI CAC'GTC
Cuts at: 1318 2715 1318
Size: 1397 4865
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2216 2997 3993
Size: 416 53 83 399 24 799 781 996
Cuts at: 3993 4951 442
Size: 958 1753
Fragments arranged by size:
1753 996 958 799 781 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6262
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2332 3153 3414 2332
Size: 821 261 5180
Fragments arranged by size:
5180 821 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2717 4922 1239
Size: 965 513 2205 2579
Fragments arranged by size:
2579 2205 965 513
Bpu10I CC'TnA_GC
Cuts at: 2057 2624 2765 3344 2057
Size: 567 141 579 4975
Fragments arranged by size:
4975 579 567 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2828 3152 4387 5252 5550 2828
Size: 324 1235 865 298 3540
Fragments arranged by size:
3540 1235 865 324 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2558 4925 882
Size: 617 1059 2367 2219
Fragments arranged by size:
2367 2219 1059 617
BsaAI yAC'GTr
Cuts at: 493 1204 3559 4053 493
Size: 711 2355 494 2702
Fragments arranged by size:
2702 2355 711 494
BsaBI GATnn'nnATC
Cuts at: 6256 6256
Size: 6262
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2148 2535 2638 4069
Size: 53 83 186 936 615 387 103 1431
Cuts at: 4069 4451 5086 275
Size: 382 635 1451
Fragments arranged by size:
1451 1431 936 635 615 387 382 186
103 83 53
BsaWI w'CCGG_w
Cuts at: 2745 4695 5093 5367 5514 5925 2745
Size: 1950 398 274 147 411 3082
Fragments arranged by size:
3082 1950 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6037
Fragments arranged by size:
6037 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 2734 2771 2774 3482 3491 3494 3497
Size: 1504 44 37 3 708 9 3 3
Cuts at: 3497 3506 3509 3512 1186
Size: 9 3 3 3936
Fragments arranged by size:
3936 1504 708 44 37 9 9 3
3 3 3 3
BseYI C'CCAG_C
Cuts at: 2032 2299 2568 5465 5961 2032
Size: 267 269 2897 496 2333
Fragments arranged by size:
2897 2333 496 269 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2713 3283 1163
Size: 573 977 570 4142
Fragments arranged by size:
4142 977 573 570
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2022 3712 4473 5501 1540
Size: 275 186 21 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186 21
BsiHKAI G_wGCw'C
Cuts at: 2454 3139 3345 4323 4408 5479 2454
Size: 685 206 978 85 1071 3237
Fragments arranged by size:
3237 1071 978 685 206 85
BsiWI C'GTAC_G
Cuts at: 3447 3447
Size: 6262
BsmI GAATG_Cn'
Cuts at: 3773 3866 3773
Size: 93 6169
BsmBI CGTCTCn'nnnn_
Cuts at: 2155 2155
Size: 6262
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2306 2454 2635 2685 3119 3139 3345
Size: 399 516 148 181 50 434 20 206
Cuts at: 3345 3581 4323 4408 5479 1391
Size: 236 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 516 434 399 236 206
181 148 85 50 20
BspEI T'CCGG_A
Cuts at: 5925 5925
Size: 6262
BspHI T'CATG_A
Cuts at: 3363 4151 5881 3363
Size: 788 1730 3744
Fragments arranged by size:
3744 1730 788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3617 844
Size: 2773 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2500 3384 4149 1394
Size: 429 677 884 765 3507
Fragments arranged by size:
3507 884 765 677 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3283 4757 4931 65
Size: 1183 2035 1474 174 1396
Fragments arranged by size:
2035 1474 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3013 4906 5093 1700
Size: 110 1203 1893 187 2869
Fragments arranged by size:
2869 1893 1203 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6262
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6262
BssSI C'ACGA_G
Cuts at: 4322 5334 4322
Size: 1012 5250
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2079 794
Size: 1285 4977
BstBI TT'CG_AA
Cuts at: 5066 5066
Size: 6262
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6262
BstXI CCAn_nnnn'nTGG
Cuts at: 2182 2182
Size: 6262
BstYI r'GATC_y
Cuts at: 1972 2157 2340 3592 3959 4344 4361 5813 5899
Size: 185 183 1252 367 385 17 1452 86
Cuts at: 5899 5911 6257 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1252 385 367 346 185 183
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2739 513
Size: 552 939 45 690 4036
Fragments arranged by size:
4036 939 690 552 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5523
Fragments arranged by size:
5523 683 56
BtsI GCAGTG_nn'
Cuts at: 3529 3778 4572 4592 3529
Size: 249 794 20 5199
Fragments arranged by size:
5199 794 249 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6262
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6227
DraI TTT'AAA
Cuts at: 3566 3566
Size: 6262
DraIII CAC_nnn'GTG
Cuts at: 2224 2224
Size: 6262
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3014 3451 5269 809
Size: 507 1698 437 1818 1802
Fragments arranged by size:
1818 1802 1698 507 437
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2019 3709 4598
Size: 54 1425 50 275 18 189 1690 889
Cuts at: 4598 6227 8
Size: 1629 43
Fragments arranged by size:
1690 1629 1425 889 275 189 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2019 3709 1537
Size: 275 207 1690 4090
Fragments arranged by size:
4090 1690 275 207
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 3246 3483 4192 1353
Size: 914 979 237 709 3423
Fragments arranged by size:
3423 979 914 709 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 3444 4849 5223 5369 387
Size: 1064 138 438 1417 1405 374 146 1280
Fragments arranged by size:
1417 1405 1280 1064 438 374 146 138
EcoICRI GAG'CTC
Cuts at: 3343 3343
Size: 6262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2224 2717 3122 4325 4922 5709
Size: 393 572 20 493 405 1203 597 787
Cuts at: 5709 5977 1239
Size: 268 1524
Fragments arranged by size:
1524 1203 787 597 572 493 405 393
268 20
EcoNI CCTnn'n_nnAGG
Cuts at: 3104 3104
Size: 6262
EcoO109I rG'GnC_Cy
Cuts at: 2206 2885 3109 3198 2206
Size: 679 224 89 5270
Fragments arranged by size:
5270 679 224 89
EcoRI G'AATT_C
Cuts at: 3292 3571 3949 3292
Size: 279 378 5605
Fragments arranged by size:
5605 378 279
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2494 2494
Size: 6262
FspI TGC'GCA
Cuts at: 4768 4768
Size: 6262
HaeII r_GCGC'y
Cuts at: 1678 2641 5409 1678
Size: 963 2768 2531
Fragments arranged by size:
2768 2531 963
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3341 3373 4903 4935 4977
Size: 32 135 32 1769 32 1530 32 42
Cuts at: 4977 5009 1373
Size: 32 2626
Fragments arranged by size:
2626 1769 1530 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2662 3606 3852 669
Size: 988 281 724 944 246 3079
Fragments arranged by size:
3079 988 944 724 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6262
HpaI GTT'AAC
Cuts at: 3852 3852
Size: 6262
KasI G'GCGC_C
Cuts at: 2637 2637
Size: 6262
KpnI G_GTAC'C
Cuts at: 3587 3587
Size: 6262
MfeI C'AATT_G
Cuts at: 3861 3861
Size: 6262
MluI A'CGCG_T
Cuts at: 6138 6138
Size: 6262
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3540 5376 5560 1305
Size: 2235 1836 184 2007
Fragments arranged by size:
2235 2007 1836 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4440
Fragments arranged by size:
4440 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3276 4220 4579 4738 518
Size: 648 2110 944 359 159 2042
Fragments arranged by size:
2110 2042 944 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3442 3678 4355 5503 5748 1606
Size: 1836 236 677 1148 245 2120
Fragments arranged by size:
2120 1836 1148 677 245 236
NarI GG'CG_CC
Cuts at: 2638 2638
Size: 6262
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4771
Fragments arranged by size:
4771 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6262
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6262
NotI GC'GGCC_GC
Cuts at: 3709 3709
Size: 6262
NsiI A_TGCA'T
Cuts at: 3096 3096
Size: 6262
NspI r_CATG'y
Cuts at: 1132 1229 3620 5165 1132
Size: 97 2391 1545 2229
Fragments arranged by size:
2391 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6262
PciI A'CATG_T
Cuts at: 5161 5161
Size: 6262
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2398 1108
Size: 1290 4972
PfoI T'CCnGG_A
Cuts at: 2153 3189 2153
Size: 1036 5226
PmeI GTTT'AAAC
Cuts at: 3566 3566
Size: 6262
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4331 4363 5844 5876 4331
Size: 32 1481 32 4717
Fragments arranged by size:
4717 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 2206
Size: 6262
PshAI GACnn'nnGTC
Cuts at: 1613 2784 1613
Size: 1171 5091
PsiI TTA'TAA
Cuts at: 3832 3832
Size: 6262
PspOMI G'GGCC_C
Cuts at: 3115 3115
Size: 6262
PstI C_TGCA'G
Cuts at: 830 2707 3614 830
Size: 1877 907 3478
Fragments arranged by size:
3478 1877 907
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6262
PvuII CAG'CTG
Cuts at: 3442 3442
Size: 6262
SacI G_AGCT'C
Cuts at: 3345 3345
Size: 6262
SalI G'TCGA_C
Cuts at: 1936 3604 1936
Size: 1668 4594
SbfI CC_TGCA'GG
Cuts at: 3614 3614
Size: 6262
ScaI AGT'ACT
Cuts at: 2396 4510 6222 2396
Size: 2114 1712 2436
Fragments arranged by size:
2436 2114 1712
SfcI C'TryA_G
Cuts at: 826 1045 2703 3043 3610 4745 5426 5617 826
Size: 219 1658 340 567 1135 681 191 1471
Fragments arranged by size:
1658 1471 1135 681 567 340 219 191
SfoI GGC'GCC
Cuts at: 2639 2639
Size: 6262
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6262
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6262
SmaI CCC'GGG
Cuts at: 1872 2094 3589 1872
Size: 222 1495 4545
Fragments arranged by size:
4545 1495 222
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2843 3167 4366 5267 5529 820
Size: 197 926 900 324 1199 901 262 1553
Fragments arranged by size:
1553 1199 926 901 900 324 262 197
SnaBI TAC'GTA
Cuts at: 493 4053 493
Size: 3560 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6262
SphI G_CATG'C
Cuts at: 1132 3620 1132
Size: 2488 3774
SspI AAT'ATT
Cuts at: 5 52 4186 5
Size: 47 4134 2081
Fragments arranged by size:
4134 2081 47
StuI AGG'CCT
Cuts at: 1517 2477 3394 1517
Size: 960 917 4385
Fragments arranged by size:
4385 960 917
StyI C'CwwG_G
Cuts at: 513 1065 2004 2202 2344 3417 3701 513
Size: 552 939 198 142 1073 284 3074
Fragments arranged by size:
3074 1073 939 552 284 198 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2235 2530 2847 3303 4289 4306 4459
Size: 1255 655 295 317 456 986 17 153
Cuts at: 4459 4644 5089 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 986 655 456 445 317 295
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2190 2394 3159
Size: 274 80 33 51 1443 213 204 765
Cuts at: 3159 4508 6220 96
Size: 1349 1712 138
Fragments arranged by size:
1712 1443 1349 765 274 213 204 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3118 3150 3302 3334 6222 6254 3118
Size: 32 152 32 2888 32 3126
Fragments arranged by size:
3126 2888 152 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3176 1316
Size: 1860 4402
XbaI T'CTAG_A
Cuts at: 3598 3598
Size: 6262
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 2692 2179
Size: 513 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6262
XmaI C'CCGG_G
Cuts at: 1870 2092 3587 1870
Size: 222 1495 4545
Fragments arranged by size:
4545 1495 222
XmnI GAAnn'nnTTC
Cuts at: 4391 4391
Size: 6262
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4070 276
Size: 53 83 186 936 2536 2468
Fragments arranged by size:
2536 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BstZ17I Bsu36I
EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SrfI SwaI