Restriction map for
pFN21AB9451
(Circular) (Six-base) MAPSORT of: pFN21AB9451.seq Check: 5525 from: 1 to: 5701
pFN21AB9451 5701 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3511 278
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3044 1937
Size: 1107 4594
Acc65I G'GTAC_C
Cuts at: 3022 3022
Size: 5701
AclI AA'CG_TT
Cuts at: 1563 3828 4201 1563
Size: 2265 373 3063
Fragments arranged by size:
3063 2265 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3764 5148 5416 1632
Size: 2132 1384 268 1917
Fragments arranged by size:
2132 1917 1384 268
AfeI AGC'GCT
Cuts at: 2474 2474
Size: 5701
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5504
AflIII A'CryG_T
Cuts at: 1203 4600 5577 1203
Size: 3397 977 1327
Fragments arranged by size:
3397 1327 977
AgeI A'CCGG_T
Cuts at: 4532 4532
Size: 5701
AhdI GACnn_n'nnGTC
Cuts at: 4430 4430
Size: 5701
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5701
AlwNI CAG_nnn'CTG
Cuts at: 3444 5016 3444
Size: 1572 4129
ApaI G_GGCC'C
Cuts at: 2865 2865
Size: 5701
ApaLI G'TGCA_C
Cuts at: 3758 4914 3758
Size: 1156 4545
ApoI r'AATT_y
Cuts at: 1493 3010 3236 3388 5497 1493
Size: 1517 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 226 152
AscI GG'CGCG_CC
Cuts at: 2868 2868
Size: 5701
AseI AT'TA_AT
Cuts at: 160 1032 4255 160
Size: 872 3223 1606
Fragments arranged by size:
3223 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
AvaI C'yCGr_G
Cuts at: 1870 1943 2291 2960 3026 1870
Size: 73 348 669 66 4545
Fragments arranged by size:
4545 669 348 73 66
BamHI G'GATC_C
Cuts at: 3031 3398 3031
Size: 367 5334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2009 2162 2560 2653 3022
Size: 325 217 627 222 153 398 93 369
Cuts at: 3022 4477 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 398 369 325 222 217
153 93
BanII G_rGCy'C
Cuts at: 1391 2220 2352 2787 2865 2907 3020 1391
Size: 829 132 435 78 42 113 4072
Fragments arranged by size:
4072 829 435 132 113 78 42
BbeI G_GCGC'C
Cuts at: 2166 2564 2657 2166
Size: 398 93 5210
Fragments arranged by size:
5210 398 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4730
BbvCI CC'TCA_GC
Cuts at: 2556 2556
Size: 5701
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3892 3926 1903
Size: 34 1955 34 3678
Fragments arranged by size:
3678 1955 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3593 4803 3593
Size: 1210 4491
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2294 2387 2621
Size: 107 122 71 631 605 622 93 234
Cuts at: 2621 2816 2843 4312 136
Size: 195 27 1469 1525
Fragments arranged by size:
1525 1469 631 622 605 234 195 122
107 93 71 27
BglII A'GATC_T
Cuts at: 5696 5696
Size: 5701
BlpI GC'TnA_GC
Cuts at: 2501 3118 2501
Size: 617 5084
Bme1580I G_kGCm'C
Cuts at: 1790 2014 2865 3762 4918 1790
Size: 224 851 897 1156 2573
Fragments arranged by size:
2573 1156 897 851 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5701
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2008 3432 4390
Size: 416 53 83 399 24 591 1424 958
Cuts at: 4390 442
Size: 1753
Fragments arranged by size:
1753 1424 958 591 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5701
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2866 2371
Size: 495 5206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2559 4361 1239
Size: 1320 1802 2579
Fragments arranged by size:
2579 1802 1320
Bpu10I CC'TnA_GC
Cuts at: 2556 2556
Size: 5701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3826 4691 4989 3826
Size: 865 298 4538
Fragments arranged by size:
4538 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2444 2759 4364 882
Size: 617 945 315 1605 2219
Fragments arranged by size:
2219 1605 945 617 315
BsaAI yAC'GTr
Cuts at: 493 1204 2119 3492 493
Size: 711 915 1373 2702
Fragments arranged by size:
2702 1373 915 711
BsaBI GATnn'nnATC
Cuts at: 5695 5695
Size: 5701
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2163 2243 2561 2654
Size: 53 83 186 936 630 80 318 93
Cuts at: 2654 3508 3890 4525 275
Size: 854 382 635 1451
Fragments arranged by size:
1451 936 854 635 630 382 318 186
93 83 80 53
BsaWI w'CCGG_w
Cuts at: 4134 4532 4806 4953 5364 4134
Size: 398 274 147 411 4471
Fragments arranged by size:
4471 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5476
Fragments arranged by size:
5476 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2186 2228 2386 2678 2696 2795 2813 2828
Size: 1000 42 158 292 18 99 18 15
Cuts at: 2828 2915 1186
Size: 87 3972
Fragments arranged by size:
3972 1000 292 158 99 87 42 18
18 15
BseYI C'CCAG_C
Cuts at: 2441 2786 2955 2983 4904 5400 2441
Size: 345 169 28 1921 496 2742
Fragments arranged by size:
2742 1921 496 345 169 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2986 1163
Size: 573 1250 3878
Fragments arranged by size:
3878 1250 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2151 2202 2226 2259 2277 2367
Size: 275 186 150 51 24 33 18 90
Cuts at: 2367 2391 2613 2913 3151 3912 4940 1540
Size: 24 222 300 238 761 1028 2301
Fragments arranged by size:
2301 1028 761 300 275 238 222 186
150 90 51 33 24 24 18
BsiHKAI G_wGCw'C
Cuts at: 3762 3847 4918 3762
Size: 85 1071 4545
Fragments arranged by size:
4545 1071 85
BsmI GAATG_Cn'
Cuts at: 3212 3305 3212
Size: 93 5608
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2220 2352 2787 2865 2907 3020
Size: 399 224 206 132 435 78 42 113
Cuts at: 3020 3762 3847 4918 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 435 399 224 206 132
113 85 78 42
BspEI T'CCGG_A
Cuts at: 5364 5364
Size: 5701
BspHI T'CATG_A
Cuts at: 3590 5320 3590
Size: 1730 3971
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3056 844
Size: 2212 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2235 2505 3588 1394
Size: 429 412 270 1083 3507
Fragments arranged by size:
3507 1083 429 412 270
BsrDI GCAATG_nn'
Cuts at: 65 1248 4196 4370 65
Size: 1183 2948 174 1396
Fragments arranged by size:
2948 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2272 4345 4532 1700
Size: 110 462 2073 187 2869
Fragments arranged by size:
2869 2073 462 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5701
BssHII G'CGCG_C
Cuts at: 1925 2868 1925
Size: 943 4758
BssSI C'ACGA_G
Cuts at: 3761 4773 3761
Size: 1012 4689
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5701
BstBI TT'CG_AA
Cuts at: 4505 4505
Size: 5701
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5701
BstXI CCAn_nnnn'nTGG
Cuts at: 2794 2794
Size: 5701
BstYI r'GATC_y
Cuts at: 1972 3031 3398 3783 3800 5252 5338 5350 5696
Size: 1059 367 385 17 1452 86 12 346
Cuts at: 5696 1972
Size: 1977
Fragments arranged by size:
1977 1452 1059 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2049 2145 2220 2253 2328 2361
Size: 552 939 45 96 75 33 75 33
Cuts at: 2361 2367 2895 2907 513
Size: 6 528 12 3307
Fragments arranged by size:
3307 939 552 528 96 75 75 45
33 33 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4962
Fragments arranged by size:
4962 683 56
BtsI GCAGTG_nn'
Cuts at: 3217 4011 4031 3217
Size: 794 20 4887
Fragments arranged by size:
4887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5701
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5666
DraI TTT'AAA
Cuts at: 3005 3005
Size: 5701
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4708 809
Size: 507 3392 1802
Fragments arranged by size:
3392 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2148 2199
Size: 54 1425 50 275 18 283 35 51
Cuts at: 2199 2223 2256 2274 2364 2388 2610 2910 3148
Size: 24 33 18 90 24 222 300 238
Cuts at: 3148 4037 5666 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 300 283 275 238 222
90 54 51 50 43 35 33 24
24 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 2148 2199 2223 2256 2274 2364 2388
Size: 275 336 51 24 33 18 90 24
Cuts at: 2388 2610 2910 3148 1537
Size: 222 300 238 4090
Fragments arranged by size:
4090 336 300 275 238 222 90 51
33 24 24 18
EarI CTCTTCn'nnn_
Cuts at: 1353 3631 1353
Size: 2278 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2842 4288 4662 4808 387
Size: 1064 138 1253 1446 374 146 1280
Fragments arranged by size:
1446 1280 1253 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2559 3764 4361 5148 5416 1239
Size: 393 927 1205 597 787 268 1524
Fragments arranged by size:
1524 1205 927 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2323 2845 2323
Size: 522 5179
EcoRI G'AATT_C
Cuts at: 3010 3388 3010
Size: 378 5323
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 5701
FspI TGC'GCA
Cuts at: 2935 4207 2935
Size: 1272 4429
HaeII r_GCGC'y
Cuts at: 1678 2166 2196 2476 2564 2657 2832 4848 1678
Size: 488 30 280 88 93 175 2016 2531
Fragments arranged by size:
2531 2016 488 280 175 93 88 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 4342 4374 4416
Size: 32 135 32 797 32 1941 32 42
Cuts at: 4416 4448 1373
Size: 32 2626
Fragments arranged by size:
2626 1941 797 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3045 3291 669
Size: 988 281 1107 246 3079
Fragments arranged by size:
3079 1107 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5701
HpaI GTT'AAC
Cuts at: 3291 3291
Size: 5701
KasI G'GCGC_C
Cuts at: 2162 2560 2653 2162
Size: 398 93 5210
Fragments arranged by size:
5210 398 93
KpnI G_GTAC'C
Cuts at: 3026 3026
Size: 5701
MfeI C'AATT_G
Cuts at: 3300 3300
Size: 5701
MluI A'CGCG_T
Cuts at: 5577 5577
Size: 5701
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4815 4999 1305
Size: 3510 184 2007
Fragments arranged by size:
3510 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2115 10
Size: 54 1425 343 283 3596
Fragments arranged by size:
3596 1425 343 283 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3659 4018 4177 518
Size: 648 2493 359 159 2042
Fragments arranged by size:
2493 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2051 2147 2222 2255 2285 2330 2369 2567
Size: 445 96 75 33 30 45 39 198
Cuts at: 2567 2606 2651 2675 2732 2897 2909 2987 3117
Size: 39 45 24 57 165 12 78 130
Cuts at: 3117 3794 4942 5187 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 445 245 198 165 130
96 78 75 57 45 45 39 39
33 30 24 12
NaeI GCC'GGC
Cuts at: 2274 2274
Size: 5701
NarI GG'CG_CC
Cuts at: 2163 2561 2654 2163
Size: 398 93 5210
Fragments arranged by size:
5210 398 93
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4210
Fragments arranged by size:
4210 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5701
NgoMIV G'CCGG_C
Cuts at: 2272 2272
Size: 5701
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5701
NotI GC'GGCC_GC
Cuts at: 2148 2223 2610 2910 3148 2148
Size: 75 387 300 238 4701
Fragments arranged by size:
4701 387 300 238 75
NspI r_CATG'y
Cuts at: 1132 1229 3059 4604 1132
Size: 97 1830 1545 2229
Fragments arranged by size:
2229 1830 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5701
PciI A'CATG_T
Cuts at: 4600 4600
Size: 5701
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5701
PmeI GTTT'AAAC
Cuts at: 3005 3005
Size: 5701
PmlI CAC'GTG
Cuts at: 2119 2119
Size: 5701
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3770 3802 5283 5315 3770
Size: 32 1481 32 4156
Fragments arranged by size:
4156 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5701
PsiI TTA'TAA
Cuts at: 3271 3271
Size: 5701
PspOMI G'GGCC_C
Cuts at: 2861 2861
Size: 5701
PstI C_TGCA'G
Cuts at: 830 2181 3053 830
Size: 1351 872 3478
Fragments arranged by size:
3478 1351 872
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5701
PvuII CAG'CTG
Cuts at: 2606 2606
Size: 5701
SacII CC_GC'GG
Cuts at: 2052 2148 2223 2256 2331 2370 2898 2910 2052
Size: 96 75 33 75 39 528 12 4843
Fragments arranged by size:
4843 528 96 75 75 39 33 12
SalI G'TCGA_C
Cuts at: 1936 3043 1936
Size: 1107 4594
SbfI CC_TGCA'GG
Cuts at: 3053 3053
Size: 5701
ScaI AGT'ACT
Cuts at: 3949 5661 3949
Size: 1712 3989
SfcI C'TryA_G
Cuts at: 826 1045 2177 3049 4184 4865 5056 826
Size: 219 1132 872 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 872 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2387 2816 2387
Size: 429 5272
SfoI GGC'GCC
Cuts at: 2164 2562 2655 2164
Size: 398 93 5210
Fragments arranged by size:
5210 398 93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5701
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5701
SmaI CCC'GGG
Cuts at: 1872 3028 1872
Size: 1156 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3805 4706 4968 820
Size: 197 926 1862 901 262 1553
Fragments arranged by size:
1862 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3492 493
Size: 2999 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5701
SphI G_CATG'C
Cuts at: 1132 3059 1132
Size: 1927 3774
SspI AAT'ATT
Cuts at: 5 52 3625 5
Size: 47 3573 2081
Fragments arranged by size:
3573 2081 47
StuI AGG'CCT
Cuts at: 1517 2059 1517
Size: 542 5159
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 2412 2769 3140 513
Size: 552 939 162 246 357 371 3074
Fragments arranged by size:
3074 939 552 371 357 246 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3728 3745 3898 4083 4528 325
Size: 1255 417 1731 17 153 185 445 1498
Fragments arranged by size:
1731 1498 1255 445 417 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3947 5659 96
Size: 274 80 33 51 1443 1970 1712 138
Fragments arranged by size:
1970 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5661 5693 5661
Size: 32 5669
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5701
XbaI T'CTAG_A
Cuts at: 3037 3037
Size: 5701
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5701
XmaI C'CCGG_G
Cuts at: 1870 3026 1870
Size: 1156 4545
XmnI GAAnn'nnTTC
Cuts at: 2492 3830 2492
Size: 1338 4363
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3509 276
Size: 53 83 186 936 1975 2468
Fragments arranged by size:
2468 1975 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI
NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI
SanDI SapI SexAI SrfI SwaI XcmI