Restriction map for
pFN21AB9445
(Circular) (Six-base) MAPSORT of: pFN21AB9445.seq Check: 1762 from: 1 to: 6721
pFN21AB9445 6721 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4531 278
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4064 1937
Size: 2127 4594
Acc65I G'GTAC_C
Cuts at: 4042 4042
Size: 6721
AclI AA'CG_TT
Cuts at: 1563 4848 5221 1563
Size: 3285 373 3063
Fragments arranged by size:
3285 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 4784 6168 6436 1632
Size: 1167 1985 1384 268 1917
Fragments arranged by size:
1985 1917 1384 1167 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6524
AflIII A'CryG_T
Cuts at: 1203 5620 6597 1203
Size: 4417 977 1327
Fragments arranged by size:
4417 1327 977
AgeI A'CCGG_T
Cuts at: 5552 5552
Size: 6721
AhdI GACnn_n'nnGTC
Cuts at: 5450 5450
Size: 6721
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6721
AlwNI CAG_nnn'CTG
Cuts at: 2672 3269 4464 6036 2672
Size: 597 1195 1572 3357
Fragments arranged by size:
3357 1572 1195 597
ApaLI G'TGCA_C
Cuts at: 4778 5934 4778
Size: 1156 5565
ApoI r'AATT_y
Cuts at: 1493 2129 2244 2336 3174 3627 3998 4030 4256
Size: 636 115 92 838 453 371 32 226
Cuts at: 4256 4408 6517 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 838 636 453 371 226 152
115 92 32
AseI AT'TA_AT
Cuts at: 160 1032 3116 5275 160
Size: 872 2084 2159 1606
Fragments arranged by size:
2159 2084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
AvaI C'yCGr_G
Cuts at: 1870 1943 4046 1870
Size: 73 2103 4545
Fragments arranged by size:
4545 2103 73
BamHI G'GATC_C
Cuts at: 4051 4418 4051
Size: 367 6354
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4042 5497 618
Size: 325 217 627 2255 1455 1842
Fragments arranged by size:
2255 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2140 4040 1391
Size: 749 1900 4072
Fragments arranged by size:
4072 1900 749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2127 3693 928
Size: 971 228 1566 3956
Fragments arranged by size:
3956 1566 971 228
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4912 4946 1903
Size: 34 2975 34 3678
Fragments arranged by size:
3678 2975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3579 4613 5823 3579
Size: 1034 1210 4477
Fragments arranged by size:
4477 1210 1034
BclI T'GATC_A
Cuts at: 1548 3448 1548
Size: 1900 4821
BfrBI ATG'CAT
Cuts at: 3797 3797
Size: 6721
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5332 136
Size: 107 122 71 631 605 3660 1525
Fragments arranged by size:
3660 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2232 6716 2232
Size: 4484 2237
BlpI GC'TnA_GC
Cuts at: 4138 4138
Size: 6721
Bme1580I G_kGCm'C
Cuts at: 1790 4782 5938 1790
Size: 2992 1156 2573
Fragments arranged by size:
2992 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6721
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4452 5410 442
Size: 416 53 83 399 24 3035 958 1753
Fragments arranged by size:
3035 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2706 1056
Size: 1650 5071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3019 3864 5381 1239
Size: 1780 845 1517 2579
Fragments arranged by size:
2579 1780 1517 845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3401 4846 5711 6009 3401
Size: 1445 865 298 4113
Fragments arranged by size:
4113 1445 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5384 882
Size: 617 3885 2219
Fragments arranged by size:
3885 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4512 493
Size: 711 3308 2702
Fragments arranged by size:
3308 2702 711
BsaBI GATnn'nnATC
Cuts at: 6715 6715
Size: 6721
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4528 4910 5545 275
Size: 53 83 186 936 2995 382 635 1451
Fragments arranged by size:
2995 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5154 5552 5826 5973 6384 5154
Size: 398 274 147 411 5491
Fragments arranged by size:
5491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3551 3581 3635 3665 1178
Size: 30 165 30 2148 30 54 30 4234
Fragments arranged by size:
4234 2148 165 54 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3005 3008 3011 1186
Size: 1819 3 3 4896
Fragments arranged by size:
4896 1819 3 3
BseYI C'CCAG_C
Cuts at: 2195 5924 6420 2195
Size: 3729 496 2496
Fragments arranged by size:
3729 2496 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2954 1163
Size: 573 1218 4930
Fragments arranged by size:
4930 1218 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3051 4171 4932 5960 1540
Size: 275 186 1050 1120 761 1028 2301
Fragments arranged by size:
2301 1120 1050 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4782 4867 5938 4782
Size: 85 1071 5565
Fragments arranged by size:
5565 1071 85
BsmI GAATG_Cn'
Cuts at: 4232 4325 4232
Size: 93 6628
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 4040 4782 4867 5938 1391
Size: 399 350 1900 742 85 1071 2174
Fragments arranged by size:
2174 1900 1071 742 399 350 85
BspEI T'CCGG_A
Cuts at: 6384 6384
Size: 6721
BspHI T'CATG_A
Cuts at: 4610 6340 4610
Size: 1730 4991
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3762 4076 844
Size: 2918 314 3489
Fragments arranged by size:
3489 2918 314
BsrBI CCG'CTC
Cuts at: 1394 1823 4608 1394
Size: 429 2785 3507
Fragments arranged by size:
3507 2785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3837 5216 5390 65
Size: 1183 2589 1379 174 1396
Fragments arranged by size:
2589 1396 1379 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5365 5552 1700
Size: 110 3555 187 2869
Fragments arranged by size:
3555 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6721
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6721
BssSI C'ACGA_G
Cuts at: 4781 5793 4781
Size: 1012 5709
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2699 794
Size: 1905 4816
BstBI TT'CG_AA
Cuts at: 5525 5525
Size: 6721
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6721
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 3921 2480
Size: 1441 5280
BstYI r'GATC_y
Cuts at: 1972 2232 3730 4051 4418 4803 4820 6272 6358
Size: 260 1498 321 367 385 17 1452 86
Cuts at: 6358 6370 6716 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1498 1452 385 367 346 321 260
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2086 513
Size: 552 939 82 5148
Fragments arranged by size:
5148 939 552 82
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5982
Fragments arranged by size:
5982 683 56
BtsI GCAGTG_nn'
Cuts at: 4237 5031 5051 4237
Size: 794 20 5907
Fragments arranged by size:
5907 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6721
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6686
DraI TTT'AAA
Cuts at: 3964 4025 3964
Size: 61 6660
DraIII CAC_nnn'GTG
Cuts at: 2699 2699
Size: 6721
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3050 5728 809
Size: 507 1734 2678 1802
Fragments arranged by size:
2678 1802 1734 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 3048 4168
Size: 54 1425 50 275 18 253 965 1120
Cuts at: 4168 5057 6686 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1120 965 889 275 253 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3048 4168 1537
Size: 275 1236 1120 4090
Fragments arranged by size:
4090 1236 1120 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2189 2920 2982 4651 1353
Size: 746 90 731 62 1669 3423
Fragments arranged by size:
3423 1669 746 731 90 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5308 5682 5828 387
Size: 1064 138 3719 374 146 1280
Fragments arranged by size:
3719 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2799 3019 3864 4784 5381 6168 6436
Size: 393 1167 220 845 920 597 787 268
Cuts at: 6436 1239
Size: 1524
Fragments arranged by size:
1524 1167 920 845 787 597 393 268
220
EcoNI CCTnn'n_nnAGG
Cuts at: 3363 3882 3363
Size: 519 6202
EcoO109I rG'GnC_Cy
Cuts at: 2669 2669
Size: 6721
EcoRI G'AATT_C
Cuts at: 4030 4408 4030
Size: 378 6343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3173 3173
Size: 6721
FspI TGC'GCA
Cuts at: 5227 5227
Size: 6721
HaeII r_GCGC'y
Cuts at: 1678 5868 1678
Size: 4190 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2217 2249 5362 5394 5436
Size: 32 135 32 645 32 3113 32 42
Cuts at: 5436 5468 1373
Size: 32 2626
Fragments arranged by size:
3113 2626 645 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3862 4065 4311 669
Size: 988 281 1924 203 246 3079
Fragments arranged by size:
3079 1924 988 281 246 203
HindIII A'AGCT_T
Cuts at: 748 3526 3749 748
Size: 2778 223 3720
Fragments arranged by size:
3720 2778 223
HpaI GTT'AAC
Cuts at: 4311 4311
Size: 6721
KpnI G_GTAC'C
Cuts at: 4046 4046
Size: 6721
MfeI C'AATT_G
Cuts at: 4320 4320
Size: 6721
MluI A'CGCG_T
Cuts at: 6597 6597
Size: 6721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2846 5835 6019 1305
Size: 1541 2989 184 2007
Fragments arranged by size:
2989 2007 1541 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2085 10
Size: 54 1425 343 253 4646
Fragments arranged by size:
4646 1425 343 253 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4679 5038 5197 518
Size: 648 3513 359 159 2042
Fragments arranged by size:
3513 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4137 4814 5962 6207 1606
Size: 2531 677 1148 245 2120
Fragments arranged by size:
2531 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2086 513
Size: 552 939 82 5148
Fragments arranged by size:
5148 939 552 82
NdeI CA'TA_TG
Cuts at: 387 2907 3511 387
Size: 2520 604 3597
Fragments arranged by size:
3597 2520 604
NheI G'CTAG_C
Cuts at: 1052 2702 1052
Size: 1650 5071
NotI GC'GGCC_GC
Cuts at: 4168 4168
Size: 6721
NsiI A_TGCA'T
Cuts at: 3799 3799
Size: 6721
NspI r_CATG'y
Cuts at: 1132 1229 4079 5624 1132
Size: 97 2850 1545 2229
Fragments arranged by size:
2850 2229 1545 97
PacI TTA_AT'TAA
Cuts at: 2310 2310
Size: 6721
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6721
PciI A'CATG_T
Cuts at: 5620 5620
Size: 6721
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6721
PmeI GTTT'AAAC
Cuts at: 4025 4025
Size: 6721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4790 4822 6303 6335 4790
Size: 32 1481 32 5176
Fragments arranged by size:
5176 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6721
PsiI TTA'TAA
Cuts at: 2222 2504 4004 4291 2222
Size: 282 1500 287 4652
Fragments arranged by size:
4652 1500 287 282
PstI C_TGCA'G
Cuts at: 830 4073 830
Size: 3243 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6721
SalI G'TCGA_C
Cuts at: 1936 4063 1936
Size: 2127 4594
SapI GCTCTTCn'nnn_
Cuts at: 2189 2189
Size: 6721
SbfI CC_TGCA'GG
Cuts at: 4073 4073
Size: 6721
ScaI AGT'ACT
Cuts at: 2045 3988 4969 6681 2045
Size: 1943 981 1712 2085
Fragments arranged by size:
2085 1943 1712 981
SfcI C'TryA_G
Cuts at: 826 1045 2031 2260 4069 5204 5885 6076 826
Size: 219 986 229 1809 1135 681 191 1471
Fragments arranged by size:
1809 1471 1135 986 681 229 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6721
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6721
SmaI CCC'GGG
Cuts at: 1872 4048 1872
Size: 2176 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3380 4825 5726 5988 820
Size: 197 926 1437 1445 901 262 1553
Fragments arranged by size:
1553 1445 1437 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4512 493
Size: 4019 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6721
SphI G_CATG'C
Cuts at: 1132 4079 1132
Size: 2947 3774
SspI AAT'ATT
Cuts at: 5 52 2149 2842 4645 5
Size: 47 2097 693 1803 2081
Fragments arranged by size:
2097 2081 1803 693 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6721
StyI C'CwwG_G
Cuts at: 513 1065 2004 2086 4160 513
Size: 552 939 82 2074 3074
Fragments arranged by size:
3074 2074 939 552 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2139 4748 4765 4918 5103 5548 325
Size: 1255 559 2609 17 153 185 445 1498
Fragments arranged by size:
2609 1498 1255 559 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2043 3986 4967
Size: 274 80 33 51 1443 66 1943 981
Cuts at: 4967 6679 96
Size: 1712 138
Fragments arranged by size:
1943 1712 1443 981 274 138 80 66
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6681 6713 6681
Size: 32 6689
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6721
XbaI T'CTAG_A
Cuts at: 4057 4057
Size: 6721
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6721
XmaI C'CCGG_G
Cuts at: 1870 4046 1870
Size: 2176 4545
XmnI GAAnn'nnTTC
Cuts at: 4850 4850
Size: 6721
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4529 276
Size: 53 83 186 936 2995 2468
Fragments arranged by size:
2995 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PstI PvuI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI