Restriction map for
pFN21AB9441
(Circular) (Six-base) MAPSORT of: pFN21AB9441.seq Check: 2857 from: 1 to: 6451
pFN21AB9441 6451 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4261 278
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3708 3794 1937
Size: 1771 86 4594
Fragments arranged by size:
4594 1771 86
Acc65I G'GTAC_C
Cuts at: 3772 3772
Size: 6451
AclI AA'CG_TT
Cuts at: 1563 4578 4951 1563
Size: 3015 373 3063
Fragments arranged by size:
3063 3015 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2997 3112 3368 3432 3483 4514 5898
Size: 440 925 115 256 64 51 1031 1384
Cuts at: 5898 6166 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1031 925 440 268 256 115
64 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6254
AflIII A'CryG_T
Cuts at: 1203 5350 6327 1203
Size: 4147 977 1327
Fragments arranged by size:
4147 1327 977
AgeI A'CCGG_T
Cuts at: 5282 5282
Size: 6451
AhdI GACnn_n'nnGTC
Cuts at: 5180 5180
Size: 6451
AleI CACnn'nnGTG
Cuts at: 1166 2876 3491 1166
Size: 1710 615 4126
Fragments arranged by size:
4126 1710 615
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3044 3386 1184
Size: 1860 342 4249
Fragments arranged by size:
4249 1860 342
AlwNI CAG_nnn'CTG
Cuts at: 3086 3721 4194 5766 3086
Size: 635 473 1572 3771
Fragments arranged by size:
3771 1572 635 473
ApaI G_GGCC'C
Cuts at: 3136 3136
Size: 6451
ApaLI G'TGCA_C
Cuts at: 4508 5664 4508
Size: 1156 5295
ApoI r'AATT_y
Cuts at: 1493 2264 3760 3986 4138 6247 1493
Size: 771 1496 226 152 2109 1697
Fragments arranged by size:
2109 1697 1496 771 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3656 5005 160
Size: 872 2624 1349 1606
Fragments arranged by size:
2624 1606 1349 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
AvaI C'yCGr_G
Cuts at: 1870 1943 2415 3776 1870
Size: 73 472 1361 4545
Fragments arranged by size:
4545 1361 472 73
BamHI G'GATC_C
Cuts at: 3406 3781 4148 3406
Size: 375 367 5709
Fragments arranged by size:
5709 375 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3461 3772 5227 618
Size: 325 217 627 1674 311 1455 1842
Fragments arranged by size:
1842 1674 1455 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2766 2809 2951 3125 3136 3770 1391
Size: 1375 43 142 174 11 634 4072
Fragments arranged by size:
4072 1375 634 174 142 43 11
BbeI G_GCGC'C
Cuts at: 3465 3465
Size: 6451
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2150 3047 3489 928
Size: 971 251 897 442 3890
Fragments arranged by size:
3890 971 897 442 251
BbvCI CC'TCA_GC
Cuts at: 3349 3349
Size: 6451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2292 2326 2396 2430 4642 4676 1903
Size: 34 355 34 70 34 2212 34 3678
Fragments arranged by size:
3678 2212 355 70 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3643 4343 5553 3643
Size: 700 1210 4541
Fragments arranged by size:
4541 1210 700
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6451
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5062 136
Size: 107 122 71 631 605 337 3053 1525
Fragments arranged by size:
3053 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3542 6446 3542
Size: 2904 3547
BlpI GC'TnA_GC
Cuts at: 2486 3092 3868 2486
Size: 606 776 5069
Fragments arranged by size:
5069 776 606
Bme1580I G_kGCm'C
Cuts at: 1790 3136 4512 5668 1790
Size: 1346 1376 1156 2573
Fragments arranged by size:
2573 1376 1346 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6451
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4182 5140 442
Size: 416 53 83 399 24 2765 958 1753
Fragments arranged by size:
2765 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3290 1056
Size: 2234 4217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 6451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 2524 2874 3173 3629 5111 1239
Size: 1259 26 350 299 456 1482 2579
Fragments arranged by size:
2579 1482 1259 456 350 299 26
Bpu10I CC'TnA_GC
Cuts at: 3087 3349 3359 3087
Size: 262 10 6179
Fragments arranged by size:
6179 262 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3575 4576 5441 5739 3575
Size: 1001 865 298 4287
Fragments arranged by size:
4287 1001 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3066 5114 882
Size: 617 1567 2048 2219
Fragments arranged by size:
2219 2048 1567 617
BsaAI yAC'GTr
Cuts at: 493 1204 4242 493
Size: 711 3038 2702
Fragments arranged by size:
3038 2702 711
BsaBI GATnn'nnATC
Cuts at: 6445 6445
Size: 6451
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3462 4258 4640 5275
Size: 53 83 186 936 1929 796 382 635
Cuts at: 5275 275
Size: 1451
Fragments arranged by size:
1929 1451 936 796 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4884 5282 5556 5703 6114 4884
Size: 398 274 147 411 5221
Fragments arranged by size:
5221 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6226
Fragments arranged by size:
6226 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2402 2817 3570 1186
Size: 1216 415 753 4067
Fragments arranged by size:
4067 1216 753 415
BseYI C'CCAG_C
Cuts at: 2024 2403 2432 3002 5654 6150 2024
Size: 379 29 570 2652 496 2325
Fragments arranged by size:
2652 2325 570 496 379 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2973 3443 1163
Size: 573 1237 470 4171
Fragments arranged by size:
4171 1237 573 470
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3901 4662 5690 1540
Size: 275 186 1900 761 1028 2301
Fragments arranged by size:
2301 1900 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4512 4597 5668 4512
Size: 85 1071 5295
Fragments arranged by size:
5295 1071 85
BsmI GAATG_Cn'
Cuts at: 3962 4055 3962
Size: 93 6358
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2766 2809 2951 3125 3136 3770 4512
Size: 399 976 43 142 174 11 634 742
Cuts at: 4512 4597 5668 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 976 742 634 399 174 142
85 43 11
BspEI T'CCGG_A
Cuts at: 6114 6114
Size: 6451
BspHI T'CATG_A
Cuts at: 3700 4340 6070 3700
Size: 640 1730 4081
Fragments arranged by size:
4081 1730 640
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3706 3806 844
Size: 2862 100 3489
Fragments arranged by size:
3489 2862 100
BsrBI CCG'CTC
Cuts at: 1394 1823 4338 1394
Size: 429 2515 3507
Fragments arranged by size:
3507 2515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3258 4946 5120 65
Size: 1183 2010 1688 174 1396
Fragments arranged by size:
2010 1688 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5095 5282 1700
Size: 110 3285 187 2869
Fragments arranged by size:
3285 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2109 96
Size: 2013 4438
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6451
BssSI C'ACGA_G
Cuts at: 4511 5523 4511
Size: 1012 5439
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6451
BstBI TT'CG_AA
Cuts at: 5255 5255
Size: 6451
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6451
BstXI CCAn_nnnn'nTGG
Cuts at: 2444 2444
Size: 6451
BstYI r'GATC_y
Cuts at: 1972 3406 3542 3781 4148 4533 4550 6002 6088
Size: 1434 136 239 367 385 17 1452 86
Cuts at: 6088 6100 6446 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1434 385 367 346 239 136
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3429 3429
Size: 6451
BtgI C'CryG_G
Cuts at: 513 1065 2004 2197 513
Size: 552 939 193 4767
Fragments arranged by size:
4767 939 552 193
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2065 487
Size: 683 56 839 4873
Fragments arranged by size:
4873 839 683 56
BtsI GCAGTG_nn'
Cuts at: 3042 3486 3498 3967 4761 4781 3042
Size: 444 12 469 794 20 4712
Fragments arranged by size:
4712 794 469 444 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6451
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6416
DraI TTT'AAA
Cuts at: 3755 3755
Size: 6451
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5458 809
Size: 507 4142 1802
Fragments arranged by size:
4142 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3898 4787 6416
Size: 54 1425 50 275 18 2068 889 1629
Cuts at: 6416 8
Size: 43
Fragments arranged by size:
2068 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3898 1537
Size: 275 2086 4090
Fragments arranged by size:
4090 2086 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2379 2658 4381 1353
Size: 1026 279 1723 3423
Fragments arranged by size:
3423 1723 1026 279
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5038 5412 5558 387
Size: 1064 138 3449 374 146 1280
Fragments arranged by size:
3449 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2498 2524 2874 2997 3112 3173
Size: 393 440 426 26 350 123 115 61
Cuts at: 3173 3368 3432 3483 3629 4514 5111 5898 6166
Size: 195 64 51 146 885 597 787 268
Cuts at: 6166 1239
Size: 1524
Fragments arranged by size:
1524 885 787 597 440 426 393 350
268 195 146 123 115 64 61 51
26
EcoO109I rG'GnC_Cy
Cuts at: 2160 3132 3133 2160
Size: 972 1 5478
Fragments arranged by size:
5478 972 1
EcoRI G'AATT_C
Cuts at: 3760 4138 3760
Size: 378 6073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2202 2202
Size: 6451
FspI TGC'GCA
Cuts at: 4957 4957
Size: 6451
HaeII r_GCGC'y
Cuts at: 1678 2015 3465 5598 1678
Size: 337 1450 2133 2531
Fragments arranged by size:
2531 2133 1450 337
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5092 5124 5166 5198 1373
Size: 32 135 32 3520 32 42 32 2626
Fragments arranged by size:
3520 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3795 4041 669
Size: 988 281 1266 591 246 3079
Fragments arranged by size:
3079 1266 988 591 281 246
HindIII A'AGCT_T
Cuts at: 748 2305 748
Size: 1557 4894
HpaI GTT'AAC
Cuts at: 4041 4041
Size: 6451
KasI G'GCGC_C
Cuts at: 3461 3461
Size: 6451
KpnI G_GTAC'C
Cuts at: 3776 3776
Size: 6451
MfeI C'AATT_G
Cuts at: 4050 4050
Size: 6451
MluI A'CGCG_T
Cuts at: 6327 6327
Size: 6451
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2951 5565 5749 1305
Size: 1646 2614 184 2007
Fragments arranged by size:
2614 2007 1646 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4629
Fragments arranged by size:
4629 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 3026 3491 4409 4768 4927 518
Size: 648 1710 150 465 918 359 159 2042
Fragments arranged by size:
2042 1710 918 648 465 359 159 150
MspA1I CmG'CkG
Cuts at: 1606 2975 3158 3278 3867 4544 5692 5937 1606
Size: 1369 183 120 589 677 1148 245 2120
Fragments arranged by size:
2120 1369 1148 677 589 245 183 120
NarI GG'CG_CC
Cuts at: 3462 3462
Size: 6451
NcoI C'CATG_G
Cuts at: 513 1065 2004 2197 513
Size: 552 939 193 4767
Fragments arranged by size:
4767 939 552 193
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6451
NheI G'CTAG_C
Cuts at: 1052 3286 1052
Size: 2234 4217
NotI GC'GGCC_GC
Cuts at: 3898 3898
Size: 6451
NspI r_CATG'y
Cuts at: 1132 1229 2924 3809 5354 1132
Size: 97 1695 885 1545 2229
Fragments arranged by size:
2229 1695 1545 885 97
PasI CC'CwG_GG
Cuts at: 1348 2546 2824 1348
Size: 1198 278 4975
Fragments arranged by size:
4975 1198 278
PciI A'CATG_T
Cuts at: 5350 5350
Size: 6451
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2136 1108
Size: 1028 5423
PfoI T'CCnGG_A
Cuts at: 2475 3645 2475
Size: 1170 5281
PmeI GTTT'AAAC
Cuts at: 3755 3755
Size: 6451
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 6033 6065 4520
Size: 32 1481 32 4906
Fragments arranged by size:
4906 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2160 2160
Size: 6451
PshAI GACnn'nnGTC
Cuts at: 1613 3058 1613
Size: 1445 5006
PsiI TTA'TAA
Cuts at: 4021 4021
Size: 6451
PspOMI G'GGCC_C
Cuts at: 3132 3132
Size: 6451
PstI C_TGCA'G
Cuts at: 830 2453 3493 3803 830
Size: 1623 1040 310 3478
Fragments arranged by size:
3478 1623 1040 310
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6451
PvuII CAG'CTG
Cuts at: 2975 3158 3278 2975
Size: 183 120 6148
Fragments arranged by size:
6148 183 120
SalI G'TCGA_C
Cuts at: 1936 3793 1936
Size: 1857 4594
SbfI CC_TGCA'GG
Cuts at: 3803 3803
Size: 6451
ScaI AGT'ACT
Cuts at: 4699 6411 4699
Size: 1712 4739
SfcI C'TryA_G
Cuts at: 826 1045 2449 3489 3799 4934 5615 5806 826
Size: 219 1404 1040 310 1135 681 191 1471
Fragments arranged by size:
1471 1404 1135 1040 681 310 219 191
SfoI GGC'GCC
Cuts at: 3463 3463
Size: 6451
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6451
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6451
SmaI CCC'GGG
Cuts at: 1872 3778 1872
Size: 1906 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3554 4555 5456 5718 820
Size: 197 926 1611 1001 901 262 1553
Fragments arranged by size:
1611 1553 1001 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4242 493
Size: 3749 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6451
SphI G_CATG'C
Cuts at: 1132 2924 3809 1132
Size: 1792 885 3774
Fragments arranged by size:
3774 1792 885
SspI AAT'ATT
Cuts at: 5 52 4375 5
Size: 47 4323 2081
Fragments arranged by size:
4323 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6451
StyI C'CwwG_G
Cuts at: 513 1065 2004 2124 2197 2496 3890 513
Size: 552 939 120 73 299 1394 3074
Fragments arranged by size:
3074 1394 939 552 299 120 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2513 2816 4478 4495 4648 4833 5278
Size: 1255 933 303 1662 17 153 185 445
Cuts at: 5278 325
Size: 1498
Fragments arranged by size:
1662 1498 1255 933 445 303 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2109 3692 4697
Size: 274 80 33 51 1443 132 1583 1005
Cuts at: 4697 6409 96
Size: 1712 138
Fragments arranged by size:
1712 1583 1443 1005 274 138 132 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6411 6443 6411
Size: 32 6419
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6451
XbaI T'CTAG_A
Cuts at: 2596 3787 2596
Size: 1191 5260
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2473 2473
Size: 6451
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6451
XmaI C'CCGG_G
Cuts at: 1870 3776 1870
Size: 1906 4545
XmnI GAAnn'nnTTC
Cuts at: 2283 3616 4580 2283
Size: 1333 964 4154
Fragments arranged by size:
4154 1333 964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4259 276
Size: 53 83 186 936 2725 2468
Fragments arranged by size:
2725 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI