Restriction map for
pFN21AB9437
(Circular) (Six-base) MAPSORT of: pFN21AB9437.seq Check: 3012 from: 1 to: 6490
pFN21AB9437 6490 bp
With 182 enzymes: SgfI *
April 28, 2014 11:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2625 2625
Size: 6490
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4300 278
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2565 3833 1937
Size: 628 1268 4594
Fragments arranged by size:
4594 1268 628
Acc65I G'GTAC_C
Cuts at: 3811 3811
Size: 6490
AclI AA'CG_TT
Cuts at: 1563 4617 4990 1563
Size: 3054 373 3063
Fragments arranged by size:
3063 3054 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4553 5937 6205 1632
Size: 2921 1384 268 1917
Fragments arranged by size:
2921 1917 1384 268
AfeI AGC'GCT
Cuts at: 2042 2042
Size: 6490
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6293
AflIII A'CryG_T
Cuts at: 1203 5389 6366 1203
Size: 4186 977 1327
Fragments arranged by size:
4186 1327 977
AgeI A'CCGG_T
Cuts at: 5321 5321
Size: 6490
AhdI GACnn_n'nnGTC
Cuts at: 5219 5219
Size: 6490
AleI CACnn'nnGTG
Cuts at: 1166 2582 1166
Size: 1416 5074
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6490
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3170 3202 3170
Size: 32 6458
AlwNI CAG_nnn'CTG
Cuts at: 2181 4233 5805 2181
Size: 2052 1572 2866
Fragments arranged by size:
2866 2052 1572
ApaI G_GGCC'C
Cuts at: 2165 2309 2165
Size: 144 6346
ApaLI G'TGCA_C
Cuts at: 2513 4547 5703 2513
Size: 2034 1156 3300
Fragments arranged by size:
3300 2034 1156
ApoI r'AATT_y
Cuts at: 1493 3465 3799 4025 4177 6286 1493
Size: 1972 334 226 152 2109 1697
Fragments arranged by size:
2109 1972 1697 334 226 152
AscI GG'CGCG_CC
Cuts at: 2496 2496
Size: 6490
AseI AT'TA_AT
Cuts at: 160 1032 5044 160
Size: 872 4012 1606
Fragments arranged by size:
4012 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
AvaI C'yCGr_G
Cuts at: 1870 1943 2145 2309 2426 3123 3668 3815 1870
Size: 73 202 164 117 697 545 147 4545
Fragments arranged by size:
4545 697 545 202 164 147 117 73
BamHI G'GATC_C
Cuts at: 3820 4187 3820
Size: 367 6123
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 2290 2402 2825 3031
Size: 325 217 627 384 119 112 423 206
Cuts at: 3031 3118 3218 3433 3629 3811 5266 618
Size: 87 100 215 196 182 1455 1842
Fragments arranged by size:
1842 1455 627 423 384 325 217 215
206 196 182 119 112 100 87
BanII G_rGCy'C
Cuts at: 1391 2100 2165 2275 2309 2417 2928 3011 3109
Size: 709 65 110 34 108 511 83 98
Cuts at: 3109 3252 3684 3708 3809 1391
Size: 143 432 24 101 4072
Fragments arranged by size:
4072 709 511 432 143 110 108 101
98 83 65 34 24
BbeI G_GCGC'C
Cuts at: 2175 2294 2406 2829 3035 2175
Size: 119 112 423 206 5630
Fragments arranged by size:
5630 423 206 119 112
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5519
BbvCI CC'TCA_GC
Cuts at: 2193 3272 2193
Size: 1079 5411
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4681 4715 1903
Size: 34 2744 34 3678
Fragments arranged by size:
3678 2744 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4382 5592 4382
Size: 1210 5280
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2169 2313 2531
Size: 107 122 71 631 605 497 144 218
Cuts at: 2531 2546 3049 3205 3623 3734 5101 136
Size: 15 503 156 418 111 1367 1525
Fragments arranged by size:
1525 1367 631 605 503 497 418 218
156 144 122 111 107 71 15
BglII A'GATC_T
Cuts at: 6485 6485
Size: 6490
BlpI GC'TnA_GC
Cuts at: 2411 2764 3907 2411
Size: 353 1143 4994
Fragments arranged by size:
4994 1143 353
Bme1580I G_kGCm'C
Cuts at: 1790 2165 2309 2517 2574 3123 3438 3634 4551
Size: 375 144 208 57 549 315 196 917
Cuts at: 4551 5707 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 917 549 375 315 208 196
144 57
BmgBI CAC'GTC
Cuts at: 1318 2779 3747 1318
Size: 1461 968 4061
Fragments arranged by size:
4061 1461 968
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2923 3126 4221
Size: 416 53 83 399 24 1506 203 1095
Cuts at: 4221 5179 442
Size: 958 1753
Fragments arranged by size:
1753 1506 1095 958 416 399 203 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6490
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3128 3128
Size: 6490
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2059 2452 3167 5150 1239
Size: 820 393 715 1983 2579
Fragments arranged by size:
2579 1983 820 715 393
Bpu10I CC'TnA_GC
Cuts at: 2193 3272 2193
Size: 1079 5411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3668 4615 5480 5778 3668
Size: 947 865 298 4380
Fragments arranged by size:
4380 947 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3062 5153 882
Size: 617 1563 2091 2219
Fragments arranged by size:
2219 2091 1563 617
BsaAI yAC'GTr
Cuts at: 493 1204 2569 3025 4281 493
Size: 711 1365 456 1256 2702
Fragments arranged by size:
2702 1365 1256 711 456
BsaBI GATnn'nnATC
Cuts at: 6484 6484
Size: 6490
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 2172 2189 2291
Size: 53 83 186 936 531 108 17 102
Cuts at: 2291 2403 2826 3032 3041 3197 3717 4297 4679
Size: 112 423 206 9 156 520 580 382
Cuts at: 4679 5314 275
Size: 635 1451
Fragments arranged by size:
1451 936 635 580 531 520 423 382
206 186 156 112 108 102 83 53
17 9
BsaWI w'CCGG_w
Cuts at: 3760 4923 5321 5595 5742 6153 3760
Size: 1163 398 274 147 411 4097
Fragments arranged by size:
4097 1163 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2429 2459 3140 3170 3200
Size: 30 165 30 1026 30 681 30 30
Cuts at: 3200 1178
Size: 4468
Fragments arranged by size:
4468 1026 681 165 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2125 2453 1186
Size: 939 328 5223
Fragments arranged by size:
5223 939 328
BseYI C'CCAG_C
Cuts at: 2328 2804 5693 6189 2328
Size: 476 2889 496 2629
Fragments arranged by size:
2889 2629 496 476
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3065 3156 1163
Size: 573 1329 91 4497
Fragments arranged by size:
4497 1329 573 91
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2217 2373 2484 2526 2694 2920
Size: 275 186 216 156 111 42 168 226
Cuts at: 2920 2949 2957 3738 3761 3940 4701 5729 1540
Size: 29 8 781 23 179 761 1028 2301
Fragments arranged by size:
2301 1028 781 761 275 226 216 186
179 168 156 111 42 29 23 8
BsiHKAI G_wGCw'C
Cuts at: 2517 3662 3684 4551 4636 5707 2517
Size: 1145 22 867 85 1071 3300
Fragments arranged by size:
3300 1145 1071 867 85 22
BsmI GAATG_Cn'
Cuts at: 4001 4094 4001
Size: 93 6397
BsmBI CGTCTCn'nnnn_
Cuts at: 2785 2785
Size: 6490
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2165 2275 2309 2417 2517 2574
Size: 399 310 65 110 34 108 100 57
Cuts at: 2574 2928 3011 3109 3123 3252 3438 3634 3662
Size: 354 83 98 14 129 186 196 28
Cuts at: 3662 3684 3708 3809 4551 4636 5707 1391
Size: 22 24 101 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 354 310 196 186
129 110 108 101 100 98 85 83
65 57 34 28 24 22 14
BspEI T'CCGG_A
Cuts at: 6153 6153
Size: 6490
BspHI T'CATG_A
Cuts at: 4379 6109 4379
Size: 1730 4760
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2625 3150 3845 844
Size: 1781 525 695 3489
Fragments arranged by size:
3489 1781 695 525
BsrBI CCG'CTC
Cuts at: 1394 1823 2158 2232 2462 2886 3112 4377 1394
Size: 429 335 74 230 424 226 1265 3507
Fragments arranged by size:
3507 1265 429 424 335 230 226 74
BsrDI GCAATG_nn'
Cuts at: 65 1248 3406 4985 5159 65
Size: 1183 2158 1579 174 1396
Fragments arranged by size:
2158 1579 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3221 3276 3733 5134 5321 1700
Size: 110 1411 55 457 1401 187 2869
Fragments arranged by size:
2869 1411 1401 457 187 110 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6490
BssHII G'CGCG_C
Cuts at: 1925 2496 2664 2786 1925
Size: 571 168 122 5629
Fragments arranged by size:
5629 571 168 122
BssSI C'ACGA_G
Cuts at: 4550 5562 4550
Size: 1012 5478
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3088 3243 794
Size: 2294 155 4041
Fragments arranged by size:
4041 2294 155
BstBI TT'CG_AA
Cuts at: 5294 5294
Size: 6490
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6490
BstXI CCAn_nnnn'nTGG
Cuts at: 2584 2584
Size: 6490
BstYI r'GATC_y
Cuts at: 1972 3820 4187 4572 4589 6041 6127 6139 6485
Size: 1848 367 385 17 1452 86 12 346
Cuts at: 6485 1972
Size: 1977
Fragments arranged by size:
1977 1848 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2682 3619 2682
Size: 937 5553
BtgI C'CryG_G
Cuts at: 513 1065 2004 2052 2278 2398 2526 2541 2742
Size: 552 939 48 226 120 128 15 201
Cuts at: 2742 2769 2814 2910 3427 513
Size: 27 45 96 517 3576
Fragments arranged by size:
3576 939 552 517 226 201 128 120
96 48 45 27 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5751
Fragments arranged by size:
5751 683 56
BtsI GCAGTG_nn'
Cuts at: 2638 2869 4006 4800 4820 2638
Size: 231 1137 794 20 4308
Fragments arranged by size:
4308 1137 794 231 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6490
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3388 3423 529
Size: 35 2824 35 3596
Fragments arranged by size:
3596 2824 35 35
DraI TTT'AAA
Cuts at: 3794 3794
Size: 6490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5497 809
Size: 507 4181 1802
Fragments arranged by size:
4181 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2214 2481 2523
Size: 54 1425 50 275 18 384 267 42
Cuts at: 2523 2550 2691 2817 2907 2917 2946 2954 3223
Size: 27 141 126 90 10 29 8 269
Cuts at: 3223 3735 3786 3937 4826 6455 8
Size: 512 51 151 889 1629 43
Fragments arranged by size:
1629 1425 889 512 384 275 269 267
151 141 126 90 54 51 50 43
42 29 27 18 10 8
EagI C'GGCC_G
Cuts at: 1537 1812 2214 2481 2523 2691 2917 2946 2954
Size: 275 402 267 42 168 226 29 8
Cuts at: 2954 3735 3937 1537
Size: 781 202 4090
Fragments arranged by size:
4090 781 402 275 267 226 202 168
42 29 8
EarI CTCTTCn'nnn_
Cuts at: 1353 2128 3568 4420 1353
Size: 775 1440 852 3423
Fragments arranged by size:
3423 1440 852 775
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2166 5077 5451 5597 387
Size: 1064 138 577 2911 374 146 1280
Fragments arranged by size:
2911 1280 1064 577 374 146 138
EcoICRI GAG'CTC
Cuts at: 3682 3682
Size: 6490
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2059 2452 3167 4553 5150 5937 6205
Size: 393 427 393 715 1386 597 787 268
Cuts at: 6205 1239
Size: 1524
Fragments arranged by size:
1524 1386 787 715 597 427 393 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 3146 3146
Size: 6490
EcoO109I rG'GnC_Cy
Cuts at: 2161 2162 2306 2747 3128 3151 2161
Size: 1 144 441 381 23 5500
Fragments arranged by size:
5500 441 381 144 23 1
EcoRI G'AATT_C
Cuts at: 3465 3799 4177 3465
Size: 334 378 5778
Fragments arranged by size:
5778 378 334
FspI TGC'GCA
Cuts at: 4996 4996
Size: 6490
HaeII r_GCGC'y
Cuts at: 1678 2044 2175 2294 2406 2760 2829 3035 5637
Size: 366 131 119 112 354 69 206 2602
Cuts at: 5637 1678
Size: 2531
Fragments arranged by size:
2602 2531 366 354 206 131 119 112
69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3140 3172 3649 3681 5131
Size: 32 135 32 1568 32 477 32 1450
Cuts at: 5131 5163 5205 5237 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1568 1450 477 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3834 4080 669
Size: 988 281 1896 246 3079
Fragments arranged by size:
3079 1896 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6490
HpaI GTT'AAC
Cuts at: 4080 4080
Size: 6490
KasI G'GCGC_C
Cuts at: 2171 2290 2402 2825 3031 2171
Size: 119 112 423 206 5630
Fragments arranged by size:
5630 423 206 119 112
KpnI G_GTAC'C
Cuts at: 3815 3815
Size: 6490
MfeI C'AATT_G
Cuts at: 4089 4089
Size: 6490
MluI A'CGCG_T
Cuts at: 6366 6366
Size: 6490
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3575 5604 5788 1305
Size: 2270 2029 184 2007
Fragments arranged by size:
2270 2029 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2819 10
Size: 54 1425 343 987 3681
Fragments arranged by size:
3681 1425 987 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2582 3542 4448 4807 4966 518
Size: 648 1416 960 906 359 159 2042
Fragments arranged by size:
2042 1416 960 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2400 2528 2543 2715 2744 2771 2959
Size: 448 346 128 15 172 29 27 188
Cuts at: 2959 3202 3906 4583 5731 5976 1606
Size: 243 704 677 1148 245 2120
Fragments arranged by size:
2120 1148 704 677 448 346 245 243
188 172 128 29 27 15
NaeI GCC'GGC
Cuts at: 3278 3735 3278
Size: 457 6033
NarI GG'CG_CC
Cuts at: 2172 2291 2403 2826 3032 2172
Size: 119 112 423 206 5630
Fragments arranged by size:
5630 423 206 119 112
NcoI C'CATG_G
Cuts at: 513 1065 2004 2814 2910 3427 513
Size: 552 939 810 96 517 3576
Fragments arranged by size:
3576 939 810 552 517 96
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6490
NgoMIV G'CCGG_C
Cuts at: 3276 3733 3276
Size: 457 6033
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6490
NotI GC'GGCC_GC
Cuts at: 2523 2917 2954 3937 2523
Size: 394 37 983 5076
Fragments arranged by size:
5076 983 394 37
NspI r_CATG'y
Cuts at: 1132 1229 3848 5393 1132
Size: 97 2619 1545 2229
Fragments arranged by size:
2619 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6490
PciI A'CATG_T
Cuts at: 5389 5389
Size: 6490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3366 1108
Size: 2258 4232
PmeI GTTT'AAAC
Cuts at: 3794 3794
Size: 6490
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3170 3202 4559 4591 6072 6104 3170
Size: 32 1357 32 1481 32 3556
Fragments arranged by size:
3556 1481 1357 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3128 3151 3128
Size: 23 6467
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6490
PsiI TTA'TAA
Cuts at: 4060 4060
Size: 6490
PspOMI G'GGCC_C
Cuts at: 2161 2305 2161
Size: 144 6346
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2954 2986 2954
Size: 32 6458
PstI C_TGCA'G
Cuts at: 830 2622 3093 3842 830
Size: 1792 471 749 3478
Fragments arranged by size:
3478 1792 749 471
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6490
SacI G_AGCT'C
Cuts at: 3684 3684
Size: 6490
SacII CC_GC'GG
Cuts at: 2055 2401 2529 2544 2745 2772 2055
Size: 346 128 15 201 27 5773
Fragments arranged by size:
5773 346 201 128 27 15
SalI G'TCGA_C
Cuts at: 1936 3832 1936
Size: 1896 4594
SanDI GG'GwC_CC
Cuts at: 3128 3128
Size: 6490
SbfI CC_TGCA'GG
Cuts at: 2622 3842 2622
Size: 1220 5270
ScaI AGT'ACT
Cuts at: 4738 6450 4738
Size: 1712 4778
SfcI C'TryA_G
Cuts at: 826 1045 2618 3089 3253 3612 3838 4973 5654
Size: 219 1573 471 164 359 226 1135 681
Cuts at: 5654 5845 826
Size: 191 1471
Fragments arranged by size:
1573 1471 1135 681 471 359 226 219
191 164
SfoI GGC'GCC
Cuts at: 2173 2292 2404 2827 3033 2173
Size: 119 112 423 206 5630
Fragments arranged by size:
5630 423 206 119 112
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6490
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6490
SmaI CCC'GGG
Cuts at: 1872 2147 2311 3125 3817 1872
Size: 275 164 814 692 4545
Fragments arranged by size:
4545 814 692 275 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3683 4594 5495 5757 820
Size: 197 926 1740 911 901 262 1553
Fragments arranged by size:
1740 1553 926 911 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4281 493
Size: 3788 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6490
SphI G_CATG'C
Cuts at: 1132 3848 1132
Size: 2716 3774
SspI AAT'ATT
Cuts at: 5 52 4414 5
Size: 47 4362 2081
Fragments arranged by size:
4362 2081 47
StuI AGG'CCT
Cuts at: 1517 2687 1517
Size: 1170 5320
StyI C'CwwG_G
Cuts at: 513 1065 2004 2814 2910 3427 3929 513
Size: 552 939 810 96 517 502 3074
Fragments arranged by size:
3074 939 810 552 517 502 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4517 4534 4687 4872 5317 325
Size: 1255 2937 17 153 185 445 1498
Fragments arranged by size:
2937 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2931 4736 6448
Size: 274 80 33 51 1443 954 1805 1712
Cuts at: 6448 96
Size: 138
Fragments arranged by size:
1805 1712 1443 954 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 6450 6482 3755
Size: 32 2663 32 3763
Fragments arranged by size:
3763 2663 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6490
XbaI T'CTAG_A
Cuts at: 3826 3826
Size: 6490
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2581 2812 2581
Size: 231 6259
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6490
XmaI C'CCGG_G
Cuts at: 1870 2145 2309 3123 3815 1870
Size: 275 164 814 692 4545
Fragments arranged by size:
4545 814 692 275 164
XmnI GAAnn'nnTTC
Cuts at: 2042 4619 2042
Size: 2577 3913
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4298 276
Size: 53 83 186 936 2764 2468
Fragments arranged by size:
2764 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacI SacII SalI SanDI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PvuII RsrII SapI SexAI SfiI SrfI SwaI