Restriction map for
pFN21AB9431
(Circular) (Six-base) MAPSORT of: pFN21AB9431.seq Check: 918 from: 1 to: 6277
pFN21AB9431 6277 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2126 2357 2126
Size: 231 6046
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4087 278
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3264 3620 1937
Size: 1327 356 4594
Fragments arranged by size:
4594 1327 356
Acc65I G'GTAC_C
Cuts at: 3598 3598
Size: 6277
AclI AA'CG_TT
Cuts at: 1563 4404 4777 1563
Size: 2841 373 3063
Fragments arranged by size:
3063 2841 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2444 3000 3164 3564 4340 5724 5992 1632
Size: 812 556 164 400 776 1384 268 1917
Fragments arranged by size:
1917 1384 812 776 556 400 268 164
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6080
AflIII A'CryG_T
Cuts at: 1203 5176 6153 1203
Size: 3973 977 1327
Fragments arranged by size:
3973 1327 977
AgeI A'CCGG_T
Cuts at: 2844 5108 2844
Size: 2264 4013
AhdI GACnn_n'nnGTC
Cuts at: 5006 5006
Size: 6277
AleI CACnn'nnGTG
Cuts at: 1166 3431 1166
Size: 2265 4012
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2628 1184
Size: 1444 4833
AlwNI CAG_nnn'CTG
Cuts at: 2593 2641 3489 4020 5592 2593
Size: 48 848 531 1572 3278
Fragments arranged by size:
3278 1572 848 531 48
ApaI G_GGCC'C
Cuts at: 3416 3416
Size: 6277
ApaLI G'TGCA_C
Cuts at: 4334 5490 4334
Size: 1156 5121
ApoI r'AATT_y
Cuts at: 1493 3586 3812 3964 6073 1493
Size: 2093 226 152 2109 1697
Fragments arranged by size:
2109 2093 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4831 160
Size: 872 3799 1606
Fragments arranged by size:
3799 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
AvaI C'yCGr_G
Cuts at: 1870 1943 2064 3308 3602 1870
Size: 73 121 1244 294 4545
Fragments arranged by size:
4545 1244 294 121 73
BamHI G'GATC_C
Cuts at: 3607 3974 3607
Size: 367 5910
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3332 3598 5053 618
Size: 325 217 627 1545 266 1455 1842
Fragments arranged by size:
1842 1545 1455 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 2904 3246 3317 3344 3416 3483 3576 3596
Size: 1513 342 71 27 72 67 93 20
Cuts at: 3596 1391
Size: 4072
Fragments arranged by size:
4072 1513 342 93 72 71 67 27
20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2520 3057 3564 928
Size: 971 621 537 507 3641
Fragments arranged by size:
3641 971 621 537 507
BbvCI CC'TCA_GC
Cuts at: 3045 3045
Size: 6277
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3103 3137 4468 4502 1903
Size: 34 50 34 1082 34 1331 34 3678
Fragments arranged by size:
3678 1331 1082 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4169 5379 4169
Size: 1210 5067
BclI T'GATC_A
Cuts at: 1548 2149 1548
Size: 601 5676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3107 4888 136
Size: 107 122 71 631 605 1435 1781 1525
Fragments arranged by size:
1781 1525 1435 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6272 6272
Size: 6277
BlpI GC'TnA_GC
Cuts at: 3694 3694
Size: 6277
Bme1580I G_kGCm'C
Cuts at: 1790 2928 3416 4338 5494 1790
Size: 1138 488 922 1156 2573
Fragments arranged by size:
2573 1156 1138 922 488
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6277
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4008 4966
Size: 416 53 83 399 24 2060 531 958
Cuts at: 4966 442
Size: 1753
Fragments arranged by size:
2060 1753 958 531 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3194 4937 1239
Size: 1955 1743 2579
Fragments arranged by size:
2579 1955 1743
Bpu10I CC'TnA_GC
Cuts at: 2763 3045 2763
Size: 282 5995
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4402 5267 5565 4402
Size: 865 298 5114
Fragments arranged by size:
5114 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2695 3449 4940 882
Size: 617 1196 754 1491 2219
Fragments arranged by size:
2219 1491 1196 754 617
BsaAI yAC'GTr
Cuts at: 493 1204 4068 493
Size: 711 2864 2702
Fragments arranged by size:
2864 2702 711
BsaBI GATnn'nnATC
Cuts at: 6271 6271
Size: 6277
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2070 2331 2796 3515
Size: 53 83 186 936 537 261 465 719
Cuts at: 3515 4084 4466 5101 275
Size: 569 382 635 1451
Fragments arranged by size:
1451 936 719 635 569 537 465 382
261 186 83 53
BsaWI w'CCGG_w
Cuts at: 2844 4710 5108 5382 5529 5940 2844
Size: 1866 398 274 147 411 3181
Fragments arranged by size:
3181 1866 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2363 2393 1178
Size: 30 165 30 960 30 5062
Fragments arranged by size:
5062 960 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 2762 2924 2957 2960 2963 2966 2969
Size: 1291 285 162 33 3 3 3 3
Cuts at: 2969 2972 2975 2978 3002 3005 3008 3011 3140
Size: 3 3 3 24 3 3 3 129
Cuts at: 3140 3170 1186
Size: 30 4293
Fragments arranged by size:
4293 1291 285 162 129 33 30 24
3 3 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2040 2495 5480 5976 2040
Size: 455 2985 496 2341
Fragments arranged by size:
2985 2341 496 455
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 3032 1163
Size: 573 482 814 4408
Fragments arranged by size:
4408 814 573 482
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2815 3140 3727 4488 5516 1540
Size: 275 186 814 325 587 761 1028 2301
Fragments arranged by size:
2301 1028 814 761 587 325 275 186
BsiHKAI G_wGCw'C
Cuts at: 3246 3576 4338 4423 5494 3246
Size: 330 762 85 1071 4029
Fragments arranged by size:
4029 1071 762 330 85
BsmI GAATG_Cn'
Cuts at: 3788 3881 3788
Size: 93 6184
BsmBI CGTCTCn'nnnn_
Cuts at: 2077 2077
Size: 6277
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2904 2928 3246 3317 3344 3416 3483
Size: 399 1114 24 318 71 27 72 67
Cuts at: 3483 3576 3596 4338 4423 5494 1391
Size: 93 20 742 85 1071 2174
Fragments arranged by size:
2174 1114 1071 742 399 318 93 85
72 71 67 27 24 20
BspEI T'CCGG_A
Cuts at: 5940 5940
Size: 6277
BspHI T'CATG_A
Cuts at: 4166 5896 4166
Size: 1730 4547
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2126 2357 3632 844
Size: 1282 231 1275 3489
Fragments arranged by size:
3489 1282 1275 231
BsrBI CCG'CTC
Cuts at: 1394 1823 3136 3423 4164 1394
Size: 429 1313 287 741 3507
Fragments arranged by size:
3507 1313 741 429 287
BsrDI GCAATG_nn'
Cuts at: 65 1248 4772 4946 65
Size: 1183 3524 174 1396
Fragments arranged by size:
3524 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2844 3031 4921 5108 1700
Size: 110 1034 187 1890 187 2869
Fragments arranged by size:
2869 1890 1034 187 187 110
BsrGI T'GTAC_A
Cuts at: 96 3225 96
Size: 3129 3148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6277
BssSI C'ACGA_G
Cuts at: 4337 5349 4337
Size: 1012 5265
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2882 3402 794
Size: 2088 520 3669
Fragments arranged by size:
3669 2088 520
BstBI TT'CG_AA
Cuts at: 5081 5081
Size: 6277
BstEII G'GTnAC_C
Cuts at: 1184 2401 3463 1184
Size: 1217 1062 3998
Fragments arranged by size:
3998 1217 1062
BstYI r'GATC_y
Cuts at: 1972 2430 3607 3974 4359 4376 5828 5914 5926
Size: 458 1177 367 385 17 1452 86 12
Cuts at: 5926 6272 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1177 458 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2046 3459 513
Size: 552 981 1413 3331
Fragments arranged by size:
3331 1413 981 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2319 487
Size: 683 56 1093 4445
Fragments arranged by size:
4445 1093 683 56
BtsI GCAGTG_nn'
Cuts at: 2230 2855 3793 4587 4607 2230
Size: 625 938 794 20 3900
Fragments arranged by size:
3900 938 794 625 20
ClaI AT'CG_AT
Cuts at: 1554 3093 1554
Size: 1539 4738
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3310 3345 529
Size: 35 2746 35 3461
Fragments arranged by size:
3461 2746 35 35
DraI TTT'AAA
Cuts at: 3581 3581
Size: 6277
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2687 5284 809
Size: 507 1371 2597 1802
Fragments arranged by size:
2597 1802 1371 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2394 3033
Size: 54 1425 50 275 18 303 261 639
Cuts at: 3033 3724 4613 6242 8
Size: 691 889 1629 43
Fragments arranged by size:
1629 1425 889 691 639 303 275 261
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3724 1537
Size: 275 1912 4090
Fragments arranged by size:
4090 1912 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2595 2967 2979 4207 1353
Size: 1242 372 12 1228 3423
Fragments arranged by size:
3423 1242 1228 372 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4864 5238 5384 387
Size: 1064 138 3275 374 146 1280
Fragments arranged by size:
3275 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3244 3574 3244
Size: 330 5947
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2444 3000 3164 3194 3564 4340 4937
Size: 393 812 556 164 30 370 776 597
Cuts at: 4937 5724 5992 1239
Size: 787 268 1524
Fragments arranged by size:
1524 812 787 776 597 556 393 370
268 164 30
EcoNI CCTnn'n_nnAGG
Cuts at: 2438 2438
Size: 6277
EcoO109I rG'GnC_Cy
Cuts at: 2061 2767 3412 3413 2061
Size: 706 645 1 4925
Fragments arranged by size:
4925 706 645 1
EcoRI G'AATT_C
Cuts at: 3586 3964 3586
Size: 378 5899
FspI TGC'GCA
Cuts at: 4783 4783
Size: 6277
HaeII r_GCGC'y
Cuts at: 1678 5424 1678
Size: 3746 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2395 2786 2818 4918
Size: 32 135 32 791 32 391 32 2100
Cuts at: 4918 4950 4992 5024 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2100 791 391 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2158 2281 3621 3867 669
Size: 988 281 220 123 1340 246 3079
Fragments arranged by size:
3079 1340 988 281 246 220 123
HindIII A'AGCT_T
Cuts at: 748 3284 748
Size: 2536 3741
HpaI GTT'AAC
Cuts at: 3867 3867
Size: 6277
KpnI G_GTAC'C
Cuts at: 3602 3602
Size: 6277
MfeI C'AATT_G
Cuts at: 3876 3876
Size: 6277
MluI A'CGCG_T
Cuts at: 6153 6153
Size: 6277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2295 5391 5575 1305
Size: 990 3096 184 2007
Fragments arranged by size:
3096 2007 990 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4455
Fragments arranged by size:
4455 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3431 4235 4594 4753 518
Size: 648 2265 804 359 159 2042
Fragments arranged by size:
2265 2042 804 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2017 2328 2608 3693 4370 5518 5763 1606
Size: 411 311 280 1085 677 1148 245 2120
Fragments arranged by size:
2120 1148 1085 677 411 311 280 245
NaeI GCC'GGC
Cuts at: 3033 3033
Size: 6277
NcoI C'CATG_G
Cuts at: 513 1065 3459 513
Size: 552 2394 3331
Fragments arranged by size:
3331 2394 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6277
NgoMIV G'CCGG_C
Cuts at: 3031 3031
Size: 6277
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6277
NotI GC'GGCC_GC
Cuts at: 3724 3724
Size: 6277
NspI r_CATG'y
Cuts at: 1132 1229 3635 5180 1132
Size: 97 2406 1545 2229
Fragments arranged by size:
2406 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6277
PciI A'CATG_T
Cuts at: 5176 5176
Size: 6277
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2040 1108
Size: 932 5345
PmeI GTTT'AAAC
Cuts at: 3581 3581
Size: 6277
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4346 4378 5859 5891 4346
Size: 32 1481 32 4732
Fragments arranged by size:
4732 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6277
PsiI TTA'TAA
Cuts at: 3847 3847
Size: 6277
PspOMI G'GGCC_C
Cuts at: 3412 3412
Size: 6277
PstI C_TGCA'G
Cuts at: 830 2388 2709 3629 830
Size: 1558 321 920 3478
Fragments arranged by size:
3478 1558 920 321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6277
PvuII CAG'CTG
Cuts at: 2608 2608
Size: 6277
RsrII CG'GwC_CG
Cuts at: 2847 2847
Size: 6277
SacI G_AGCT'C
Cuts at: 3246 3576 3246
Size: 330 5947
SalI G'TCGA_C
Cuts at: 1936 3619 1936
Size: 1683 4594
SapI GCTCTTCn'nnn_
Cuts at: 2595 2595
Size: 6277
SbfI CC_TGCA'GG
Cuts at: 3629 3629
Size: 6277
ScaI AGT'ACT
Cuts at: 4525 6237 4525
Size: 1712 4565
SexAI A'CCwGG_T
Cuts at: 2517 2517
Size: 6277
SfcI C'TryA_G
Cuts at: 826 1045 2384 2489 2705 3625 4760 5441 5632
Size: 219 1339 105 216 920 1135 681 191
Cuts at: 5632 826
Size: 1471
Fragments arranged by size:
1471 1339 1135 920 681 219 216 191
105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6277
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6277
SmaI CCC'GGG
Cuts at: 1872 2066 3604 1872
Size: 194 1538 4545
Fragments arranged by size:
4545 1538 194
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4381 5282 5544 820
Size: 197 926 2438 901 262 1553
Fragments arranged by size:
2438 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4068 493
Size: 3575 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6277
SphI G_CATG'C
Cuts at: 1132 3635 1132
Size: 2503 3774
SspI AAT'ATT
Cuts at: 5 52 4201 5
Size: 47 4149 2081
Fragments arranged by size:
4149 2081 47
StuI AGG'CCT
Cuts at: 1517 3086 1517
Size: 1569 4708
StyI C'CwwG_G
Cuts at: 513 1065 2619 2904 3328 3459 3716 513
Size: 552 1554 285 424 131 257 3074
Fragments arranged by size:
3074 1554 552 424 285 257 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3565 4304 4321 4474 4659 5104 325
Size: 1255 1985 739 17 153 185 445 1498
Fragments arranged by size:
1985 1498 1255 739 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 3225 4523
Size: 274 80 33 51 1443 342 906 1298
Cuts at: 4523 6235 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1298 906 342 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2854 2886 6237 6269 2854
Size: 32 3351 32 2862
Fragments arranged by size:
3351 2862 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2816 1316
Size: 1500 4777
XbaI T'CTAG_A
Cuts at: 3613 3613
Size: 6277
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3169 3169
Size: 6277
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6277
XmaI C'CCGG_G
Cuts at: 1870 2064 3602 1870
Size: 194 1538 4545
Fragments arranged by size:
4545 1538 194
XmnI GAAnn'nnTTC
Cuts at: 4406 4406
Size: 6277
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4085 276
Size: 53 83 186 936 2551 2468
Fragments arranged by size:
2551 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI
FspAI KasI NarI NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI SacII SanDI SfiI SfoI SrfI
SwaI