Restriction map for
pFN21AB9430
(Circular) (Six-base) MAPSORT of: pFN21AB9430.seq Check: 830 from: 1 to: 6004
pFN21AB9430 6004 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2892 3347 1937
Size: 955 455 4594
Fragments arranged by size:
4594 955 455
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3130 4067 5451 5719 1632
Size: 1498 937 1384 268 1917
Fragments arranged by size:
1917 1498 1384 937 268
AfeI AGC'GCT
Cuts at: 2043 2844 2043
Size: 801 5203
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 3012 4903 5880 1203
Size: 1809 1891 977 1327
Fragments arranged by size:
1891 1809 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2696 3747 5319 2696
Size: 1051 1572 3381
Fragments arranged by size:
3381 1572 1051
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 3313 3539 3691 5800 1493
Size: 1820 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2068 2081 2149 2167 2854 3055 3236
Size: 73 125 13 68 18 687 201 181
Cuts at: 3236 3256 3269 3329 1870
Size: 20 13 60 4545
Fragments arranged by size:
4545 687 201 181 125 73 68 60
20 18 13 13
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 2567 2756 3325 4780
Size: 325 217 627 285 495 189 569 1455
Cuts at: 4780 618
Size: 1842
Fragments arranged by size:
1842 1455 627 569 495 325 285 217
189
BanII G_rGCy'C
Cuts at: 1391 2063 3062 3195 3323 1391
Size: 672 999 133 128 4072
Fragments arranged by size:
4072 999 672 133 128
BbeI G_GCGC'C
Cuts at: 2076 2571 2760 2076
Size: 495 189 5320
Fragments arranged by size:
5320 495 189
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3093 928
Size: 971 1194 3839
Fragments arranged by size:
3839 1194 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2505 2726 2822
Size: 107 122 71 631 605 833 221 96
Cuts at: 2822 4615 136
Size: 1793 1525
Fragments arranged by size:
1793 1525 833 631 605 221 122 107
96 71
BglII A'GATC_T
Cuts at: 2268 5999 2268
Size: 3731 2273
BlpI GC'TnA_GC
Cuts at: 3421 3421
Size: 6004
Bme1580I G_kGCm'C
Cuts at: 1790 2178 2915 4065 5221 1790
Size: 388 737 1150 1156 2573
Fragments arranged by size:
2573 1156 1150 737 388
BmgBI CAC'GTC
Cuts at: 1318 2916 1318
Size: 1598 4406
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3735 4693 442
Size: 416 53 83 399 24 2318 958 1753
Fragments arranged by size:
2318 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3214 3271 3284 3214
Size: 57 13 5934
Fragments arranged by size:
5934 57 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2410 4664 1239
Size: 1171 2254 2579
Fragments arranged by size:
2579 2254 1171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2339 4129 4994 5292 2339
Size: 1790 865 298 3051
Fragments arranged by size:
3051 1790 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4667 882
Size: 617 3168 2219
Fragments arranged by size:
3168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 2568 2757 3811
Size: 53 83 186 936 540 495 189 1054
Cuts at: 3811 4193 4828 275
Size: 382 635 1451
Fragments arranged by size:
1451 1054 936 635 540 495 382 189
186 83 53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2408 2414 2438 2444 2460
Size: 30 165 30 1005 6 24 6 16
Cuts at: 2460 2490 3002 3032 1178
Size: 30 512 30 4150
Fragments arranged by size:
4150 1005 512 165 30 30 30 30
24 16 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2089 2422 2437 2556 2809 3010 3028 3031
Size: 903 333 15 119 253 201 18 3
Cuts at: 3031 3040 3043 3052 1186
Size: 9 3 9 4138
Fragments arranged by size:
4138 903 333 253 201 119 18 15
9 9 3 3
BseYI C'CCAG_C
Cuts at: 2086 2281 2798 3110 5207 5703 2086
Size: 195 517 312 2097 496 2387
Fragments arranged by size:
2387 2097 517 496 312 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2030 3214 1163
Size: 573 294 1184 3953
Fragments arranged by size:
3953 1184 573 294
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2439 2938 3169 3454 4215 5243
Size: 275 186 438 499 231 285 761 1028
Cuts at: 5243 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 499 438 285 275 231
186
BsiHKAI G_wGCw'C
Cuts at: 2063 4065 4150 5221 2063
Size: 2002 85 1071 2846
Fragments arranged by size:
2846 2002 1071 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2178 2915 3062 3195 3323 4065
Size: 399 273 115 737 147 133 128 742
Cuts at: 4065 4150 5221 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 737 399 273 147 133
128 115 85
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3102 3359 844
Size: 2258 257 3489
Fragments arranged by size:
3489 2258 257
BsrBI CCG'CTC
Cuts at: 1394 1823 2224 2344 2940 3891 1394
Size: 429 401 120 596 951 3507
Fragments arranged by size:
3507 951 596 429 401 120
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2051 2075 2388 2581 2933 4648 4835
Size: 110 241 24 313 193 352 1715 187
Cuts at: 4835 1700
Size: 2869
Fragments arranged by size:
2869 1715 352 313 241 193 187 110
24
BsrGI T'GTAC_A
Cuts at: 96 2781 96
Size: 2685 3319
BssHII G'CGCG_C
Cuts at: 1925 2974 3000 1925
Size: 1049 26 4929
Fragments arranged by size:
4929 1049 26
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2696 794
Size: 1902 4102
BstBI TT'CG_AA
Cuts at: 3084 4808 3084
Size: 1724 4280
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstXI CCAn_nnnn'nTGG
Cuts at: 2281 2281
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2268 2319 3334 3701 4086 4103 5555 5641
Size: 296 51 1015 367 385 17 1452 86
Cuts at: 5641 5653 5999 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1015 385 367 346 296 86
51 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2127 2500 2956 513
Size: 552 939 123 373 456 3561
Fragments arranged by size:
3561 939 552 456 373 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2491 3520 4314 4334 2491
Size: 1029 794 20 4161
Fragments arranged by size:
4161 1029 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2461 2496 529
Size: 35 1897 35 4037
Fragments arranged by size:
4037 1897 35 35
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2049 2436 2497
Size: 54 1425 50 275 18 219 387 61
Cuts at: 2497 2935 3451 4340 5969 8
Size: 438 516 889 1629 43
Fragments arranged by size:
1629 1425 889 516 438 387 275 219
61 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2436 2935 3451 1537
Size: 275 624 499 516 4090
Fragments arranged by size:
4090 624 516 499 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3049 3934 1353
Size: 1696 885 3423
Fragments arranged by size:
3423 1696 885
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2354 2803 2885 4591 4965 5111
Size: 1064 138 765 449 82 1706 374 146
Cuts at: 5111 387
Size: 1280
Fragments arranged by size:
1706 1280 1064 765 449 374 146 138
82
EcoICRI GAG'CTC
Cuts at: 2061 2061
Size: 6004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 3130 4067 4664 5451 5719 1239
Size: 393 778 720 937 597 787 268 1524
Fragments arranged by size:
1524 937 787 778 720 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3172 3172
Size: 6004
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 2045 2076 2571 2760 2805 2846 3164 5151
Size: 367 31 495 189 45 41 318 1987
Cuts at: 5151 1678
Size: 2531
Fragments arranged by size:
2531 1987 495 367 318 189 45 41
31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2460 2492 2759 2791 3116
Size: 32 135 32 888 32 267 32 325
Cuts at: 3116 3148 4645 4677 4719 4751 1373
Size: 32 1497 32 42 32 2626
Fragments arranged by size:
2626 1497 888 325 267 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KasI G'GCGC_C
Cuts at: 2072 2567 2756 2072
Size: 495 189 5320
Fragments arranged by size:
5320 495 189
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 3012 5880 3012
Size: 2868 3136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3096 3309 5118 5302 1305
Size: 1791 213 1809 184 2007
Fragments arranged by size:
2007 1809 1791 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3962 4321 4480 518
Size: 648 2796 359 159 2042
Fragments arranged by size:
2796 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2693 2958 3420 4097 5245 5490 1606
Size: 1087 265 462 677 1148 245 2120
Fragments arranged by size:
2120 1148 1087 677 462 265 245
NaeI GCC'GGC
Cuts at: 2053 2077 2935 2053
Size: 24 858 5122
Fragments arranged by size:
5122 858 24
NarI GG'CG_CC
Cuts at: 2073 2568 2757 2073
Size: 495 189 5320
Fragments arranged by size:
5320 495 189
NcoI C'CATG_G
Cuts at: 513 1065 2004 2500 513
Size: 552 939 496 4017
Fragments arranged by size:
4017 939 552 496
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NgoMIV G'CCGG_C
Cuts at: 2051 2075 2933 2051
Size: 24 858 5122
Fragments arranged by size:
5122 858 24
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2257 3133 3362 4907 1132
Size: 97 1028 876 229 1545 2229
Fragments arranged by size:
2229 1545 1028 876 229 97
PasI CC'CwG_GG
Cuts at: 1348 2752 3136 1348
Size: 1404 384 4216
Fragments arranged by size:
4216 1404 384
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6004
PfoI T'CCnGG_A
Cuts at: 3219 3219
Size: 6004
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3098 3130 4073 4105 5586 5618 3098
Size: 32 943 32 1481 32 3484
Fragments arranged by size:
3484 1481 943 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3172 3172
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PspXI vC'TCGA_Gb
Cuts at: 2081 3269 2081
Size: 1188 4816
PstI C_TGCA'G
Cuts at: 830 3356 830
Size: 2526 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
SacI G_AGCT'C
Cuts at: 2063 2063
Size: 6004
SacII CC_GC'GG
Cuts at: 2959 2959
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 4252 5964 4252
Size: 1712 4292
SexAI A'CCwGG_T
Cuts at: 3288 3288
Size: 6004
SfcI C'TryA_G
Cuts at: 826 1045 3352 4487 5168 5359 826
Size: 219 2307 1135 681 191 1471
Fragments arranged by size:
2307 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2074 2569 2758 2074
Size: 495 189 5320
Fragments arranged by size:
5320 495 189
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 2856 3331 1872
Size: 984 475 4545
Fragments arranged by size:
4545 984 475
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 2354 3269 4108 5009 5271
Size: 197 926 138 273 915 839 901 262
Cuts at: 5271 820
Size: 1553
Fragments arranged by size:
1553 926 915 901 839 273 262 197
138
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2004 2500 3443 513
Size: 552 939 496 943 3074
Fragments arranged by size:
3074 943 939 552 496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2781 4250 5962
Size: 274 80 33 51 1443 804 1469 1712
Cuts at: 5962 96
Size: 138
Fragments arranged by size:
1712 1469 1443 804 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3002 3034 5964 5996 3002
Size: 32 2930 32 3010
Fragments arranged by size:
3010 2930 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2474 1316
Size: 1158 4846
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2659 2659
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 2081 3269 1943
Size: 138 1188 4678
Fragments arranged by size:
4678 1188 138
XmaI C'CCGG_G
Cuts at: 1870 2854 3329 1870
Size: 984 475 4545
Fragments arranged by size:
4545 984 475
XmnI GAAnn'nnTTC
Cuts at: 2198 4133 2198
Size: 1935 4069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
SacI SacII SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PsrI PvuII RsrII SanDI SapI SfiI SrfI SwaI