Restriction map for
pFN21AB9429
(Circular) (Six-base) MAPSORT of: pFN21AB9429.seq Check: 6264 from: 1 to: 6385
pFN21AB9429 6385 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4195 278
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3087 3728 1937
Size: 1150 641 4594
Fragments arranged by size:
4594 1150 641
Acc65I G'GTAC_C
Cuts at: 3706 3706
Size: 6385
AclI AA'CG_TT
Cuts at: 1563 4512 4885 1563
Size: 2949 373 3063
Fragments arranged by size:
3063 2949 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2097 2434 2864 3089 3374 4448 5832 6100
Size: 465 337 430 225 285 1074 1384 268
Cuts at: 6100 1632
Size: 1917
Fragments arranged by size:
1917 1384 1074 465 430 337 285 268
225
AfeI AGC'GCT
Cuts at: 3068 3245 3068
Size: 177 6208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6188
AflIII A'CryG_T
Cuts at: 1203 5284 6261 1203
Size: 4081 977 1327
Fragments arranged by size:
4081 1327 977
AgeI A'CCGG_T
Cuts at: 5216 5216
Size: 6385
AhdI GACnn_n'nnGTC
Cuts at: 5114 5114
Size: 6385
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3495 1184
Size: 2311 4074
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3306 3338 3306
Size: 32 6353
AlwNI CAG_nnn'CTG
Cuts at: 2023 2272 4128 5700 2023
Size: 249 1856 1572 2708
Fragments arranged by size:
2708 1856 1572 249
ApaLI G'TGCA_C
Cuts at: 4442 5598 4442
Size: 1156 5229
ApoI r'AATT_y
Cuts at: 1493 2480 2813 3694 3920 4072 6181 1493
Size: 987 333 881 226 152 2109 1697
Fragments arranged by size:
2109 1697 987 881 333 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4939 160
Size: 872 3907 1606
Fragments arranged by size:
3907 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
AvaI C'yCGr_G
Cuts at: 1870 1943 2087 3710 1870
Size: 73 144 1623 4545
Fragments arranged by size:
4545 1623 144 73
BamHI G'GATC_C
Cuts at: 3442 3715 4082 3442
Size: 273 367 5745
Fragments arranged by size:
5745 367 273
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3161 3586 3706 5161 618
Size: 325 217 627 1374 425 120 1455 1842
Fragments arranged by size:
1842 1455 1374 627 425 325 217 120
BanII G_rGCy'C
Cuts at: 1391 3704 1391
Size: 2313 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 2532 2571 2595 2870 3434 928
Size: 971 360 273 39 24 275 564 3879
Fragments arranged by size:
3879 971 564 360 275 273 39 24
BbvCI CC'TCA_GC
Cuts at: 2379 2379
Size: 6385
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2354 2388 4576 4610 1903
Size: 34 417 34 2188 34 3678
Fragments arranged by size:
3678 2188 417 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4277 5487 4277
Size: 1210 5175
BclI T'GATC_A
Cuts at: 1548 2398 1548
Size: 850 5535
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4996 136
Size: 107 122 71 631 605 3324 1525
Fragments arranged by size:
3324 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6380 6380
Size: 6385
BlpI GC'TnA_GC
Cuts at: 3285 3802 3285
Size: 517 5868
Bme1580I G_kGCm'C
Cuts at: 1790 2827 4446 5602 1790
Size: 1037 1619 1156 2573
Fragments arranged by size:
2573 1619 1156 1037
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6385
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2348 3155 4116
Size: 416 53 83 399 24 931 807 961
Cuts at: 4116 5074 442
Size: 958 1753
Fragments arranged by size:
1753 961 958 931 807 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2337 2366 2619 3053 5045 1239
Size: 1098 29 253 434 1992 2579
Fragments arranged by size:
2579 1992 1098 434 253 29
Bpu10I CC'TnA_GC
Cuts at: 2379 3273 3563 2379
Size: 894 290 5201
Fragments arranged by size:
5201 894 290
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3315 4510 5375 5673 3315
Size: 1195 865 298 4027
Fragments arranged by size:
4027 1195 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5048 882
Size: 617 3549 2219
Fragments arranged by size:
3549 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4176 493
Size: 711 2972 2702
Fragments arranged by size:
2972 2702 711
BsaBI GATnn'nnATC
Cuts at: 6379 6379
Size: 6385
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4192 4574 5209 275
Size: 53 83 186 936 2659 382 635 1451
Fragments arranged by size:
2659 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3316 4818 5216 5490 5637 6048 3316
Size: 1502 398 274 147 411 3653
Fragments arranged by size:
3653 1502 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6160
Fragments arranged by size:
6160 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2531 2534 2969 1186
Size: 1345 3 435 4602
Fragments arranged by size:
4602 1345 435 3
BseYI C'CCAG_C
Cuts at: 3050 5588 6084 3050
Size: 2538 496 3351
Fragments arranged by size:
3351 2538 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5812
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3835 4596 5624 1540
Size: 275 186 1834 761 1028 2301
Fragments arranged by size:
2301 1834 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4446 4531 5602 4446
Size: 85 1071 5229
Fragments arranged by size:
5229 1071 85
BsmI GAATG_Cn'
Cuts at: 3896 3989 3896
Size: 93 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2827 3704 4446 4531 5602 1391
Size: 399 1037 877 742 85 1071 2174
Fragments arranged by size:
2174 1071 1037 877 742 399 85
BspEI T'CCGG_A
Cuts at: 6048 6048
Size: 6385
BspHI T'CATG_A
Cuts at: 4274 6004 4274
Size: 1730 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3740 844
Size: 2896 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 4272 1394
Size: 429 1816 633 3507
Fragments arranged by size:
3507 1816 633 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4880 5054 65
Size: 1183 3632 174 1396
Fragments arranged by size:
3632 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5029 5216 1700
Size: 110 3219 187 2869
Fragments arranged by size:
3219 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6385
BssSI C'ACGA_G
Cuts at: 4445 5457 4445
Size: 1012 5373
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2272 794
Size: 1478 4907
BstBI TT'CG_AA
Cuts at: 3226 5189 3226
Size: 1963 4422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6385
BstXI CCAn_nnnn'nTGG
Cuts at: 2494 3058 3439 2494
Size: 564 381 5440
Fragments arranged by size:
5440 564 381
BstYI r'GATC_y
Cuts at: 1972 3321 3442 3715 4082 4467 4484 5936 6022
Size: 1349 121 273 367 385 17 1452 86
Cuts at: 6022 6034 6380 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1349 385 367 346 273 121
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3462 3676 513
Size: 552 939 1458 214 3222
Fragments arranged by size:
3222 1458 939 552 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5646
Fragments arranged by size:
5646 683 56
BtsI GCAGTG_nn'
Cuts at: 2097 2518 3479 3901 4695 4715 2097
Size: 421 961 422 794 20 3767
Fragments arranged by size:
3767 961 794 422 421 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6350
DraI TTT'AAA
Cuts at: 3689 3689
Size: 6385
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3083 5392 809
Size: 507 1767 2309 1802
Fragments arranged by size:
2309 1802 1767 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3459 3649 3832
Size: 54 1425 50 275 18 1629 190 183
Cuts at: 3832 4721 6350 8
Size: 889 1629 43
Fragments arranged by size:
1629 1629 1425 889 275 190 183 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3832 1537
Size: 275 2020 4090
Fragments arranged by size:
4090 2020 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2411 2526 2671 2880 2982 4315 1353
Size: 1058 115 145 209 102 1333 3423
Fragments arranged by size:
3423 1333 1058 209 145 115 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 4972 5346 5492 387
Size: 1064 138 495 2888 374 146 1280
Fragments arranged by size:
2888 1280 1064 495 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2097 2337 2366 2434 2619 2864 3053
Size: 393 465 240 29 68 185 245 189
Cuts at: 3053 3089 3374 4448 5045 5832 6100 1239
Size: 36 285 1074 597 787 268 1524
Fragments arranged by size:
1524 1074 787 597 465 393 285 268
245 240 189 185 68 36 29
EcoO109I rG'GnC_Cy
Cuts at: 3013 3013
Size: 6385
EcoRI G'AATT_C
Cuts at: 3694 4072 3694
Size: 378 6007
EcoRV GAT'ATC
Cuts at: 2918 2918
Size: 6385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2909 2909
Size: 6385
FspI TGC'GCA
Cuts at: 4891 4891
Size: 6385
HaeII r_GCGC'y
Cuts at: 1678 2030 3070 3247 5532 1678
Size: 352 1040 177 2285 2531
Fragments arranged by size:
2531 2285 1040 352 177
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2282 2297 2314 2329 3313
Size: 32 135 32 710 15 17 15 984
Cuts at: 3313 3345 5026 5058 5100 5132 1373
Size: 32 1681 32 42 32 2626
Fragments arranged by size:
2626 1681 984 710 135 42 32 32
32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2751 3729 3975 669
Size: 988 281 813 978 246 3079
Fragments arranged by size:
3079 988 978 813 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6385
HpaI GTT'AAC
Cuts at: 3975 3975
Size: 6385
KpnI G_GTAC'C
Cuts at: 3710 3710
Size: 6385
MfeI C'AATT_G
Cuts at: 3984 3984
Size: 6385
MluI A'CGCG_T
Cuts at: 6261 6261
Size: 6385
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5499 5683 1305
Size: 4194 184 2007
Fragments arranged by size:
4194 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3461 3651 10
Size: 54 1425 343 1629 190 2744
Fragments arranged by size:
2744 1629 1425 343 190 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3681 4343 4702 4861 518
Size: 648 2515 662 359 159 2042
Fragments arranged by size:
2515 2042 662 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3801 4478 5626 5871 1606
Size: 2195 677 1148 245 2120
Fragments arranged by size:
2195 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3462 513
Size: 552 939 1458 3436
Fragments arranged by size:
3436 1458 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6385
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6385
NotI GC'GGCC_GC
Cuts at: 3832 3832
Size: 6385
NspI r_CATG'y
Cuts at: 1132 1229 3743 5288 1132
Size: 97 2514 1545 2229
Fragments arranged by size:
2514 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6385
PciI A'CATG_T
Cuts at: 5284 5284
Size: 6385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 1108
Size: 1608 4777
PmeI GTTT'AAAC
Cuts at: 3689 3689
Size: 6385
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4454 4486 5967 5999 4454
Size: 32 1481 32 4840
Fragments arranged by size:
4840 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3013 3013
Size: 6385
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6385
PsiI TTA'TAA
Cuts at: 3955 3955
Size: 6385
PstI C_TGCA'G
Cuts at: 830 3737 830
Size: 2907 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6385
SalI G'TCGA_C
Cuts at: 1936 3727 1936
Size: 1791 4594
SanDI GG'GwC_CC
Cuts at: 3013 3013
Size: 6385
SapI GCTCTTCn'nnn_
Cuts at: 2411 2671 2411
Size: 260 6125
SbfI CC_TGCA'GG
Cuts at: 3737 3737
Size: 6385
ScaI AGT'ACT
Cuts at: 4633 6345 4633
Size: 1712 4673
SfcI C'TryA_G
Cuts at: 826 1045 3088 3733 4868 5549 5740 826
Size: 219 2043 645 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 645 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6385
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6385
SmaI CCC'GGG
Cuts at: 1872 3712 1872
Size: 1840 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3330 4489 5390 5652 820
Size: 197 926 1387 1159 901 262 1553
Fragments arranged by size:
1553 1387 1159 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4176 493
Size: 3683 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6385
SphI G_CATG'C
Cuts at: 1132 3743 1132
Size: 2611 3774
SspI AAT'ATT
Cuts at: 5 52 4309 5
Size: 47 4257 2081
Fragments arranged by size:
4257 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6385
StyI C'CwwG_G
Cuts at: 513 1065 2004 2487 2503 2572 3438 3462 3824
Size: 552 939 483 16 69 866 24 362
Cuts at: 3824 513
Size: 3074
Fragments arranged by size:
3074 939 866 552 483 362 69 24
16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2360 4412 4429 4582 4767 5212 325
Size: 1255 780 2052 17 153 185 445 1498
Fragments arranged by size:
2052 1498 1255 780 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4631 6343 96
Size: 274 80 33 51 1443 2654 1712 138
Fragments arranged by size:
2654 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2375 2407 2667 2699 6345 6377 2375
Size: 32 260 32 3646 32 2383
Fragments arranged by size:
3646 2383 260 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2340 1316
Size: 1024 5361
XbaI T'CTAG_A
Cuts at: 2234 3721 2234
Size: 1487 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3454 3454
Size: 6385
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6385
XmaI C'CCGG_G
Cuts at: 1870 3710 1870
Size: 1840 4545
XmnI GAAnn'nnTTC
Cuts at: 4514 4514
Size: 6385
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4193 276
Size: 53 83 186 936 2659 2468
Fragments arranged by size:
2659 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SexAI SfiI SfoI SrfI SwaI