Restriction map for
pFN21AB9426
(Circular) (Six-base) MAPSORT of: pFN21AB9426.seq Check: 3548 from: 1 to: 6754
pFN21AB9426 6754 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4564 278
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3609 3720 4097 1937
Size: 1672 111 377 4594
Fragments arranged by size:
4594 1672 377 111
Acc65I G'GTAC_C
Cuts at: 4075 4075
Size: 6754
AclI AA'CG_TT
Cuts at: 1563 4881 5254 1563
Size: 3318 373 3063
Fragments arranged by size:
3318 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2448 2810 3182 4817 6201 6469 1632
Size: 816 362 372 1635 1384 268 1917
Fragments arranged by size:
1917 1635 1384 816 372 362 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6557
AflIII A'CryG_T
Cuts at: 1203 5653 6630 1203
Size: 4450 977 1327
Fragments arranged by size:
4450 1327 977
AgeI A'CCGG_T
Cuts at: 5585 5585
Size: 6754
AhdI GACnn_n'nnGTC
Cuts at: 5483 5483
Size: 6754
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6754
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6754
AlwNI CAG_nnn'CTG
Cuts at: 2125 2326 4497 6069 2125
Size: 201 2171 1572 2810
Fragments arranged by size:
2810 2171 1572 201
ApaI G_GGCC'C
Cuts at: 3865 3865
Size: 6754
ApaLI G'TGCA_C
Cuts at: 2620 4811 5967 2620
Size: 2191 1156 3407
Fragments arranged by size:
3407 2191 1156
ApoI r'AATT_y
Cuts at: 1493 4063 4289 4441 6550 1493
Size: 2570 226 152 2109 1697
Fragments arranged by size:
2570 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5308 160
Size: 872 4276 1606
Fragments arranged by size:
4276 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2375 3915 4079 1870
Size: 73 306 126 1540 164 4545
Fragments arranged by size:
4545 1540 306 164 126 73
BamHI G'GATC_C
Cuts at: 3219 3753 4084 4451 3219
Size: 534 331 367 5522
Fragments arranged by size:
5522 534 367 331
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 2342 4075 5530 618
Size: 325 217 627 228 327 1733 1455 1842
Fragments arranged by size:
1842 1733 1455 627 327 325 228 217
BanII G_rGCy'C
Cuts at: 1391 3373 3865 4073 1391
Size: 1982 492 208 4072
Fragments arranged by size:
4072 1982 492 208
BbeI G_GCGC'C
Cuts at: 2346 2346
Size: 6754
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2195 2254 928
Size: 971 296 59 5428
Fragments arranged by size:
5428 971 296 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2611 2645 4945 4979 1903
Size: 34 674 34 2300 34 3678
Fragments arranged by size:
3678 2300 674 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4646 5856 4646
Size: 1210 5544
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6754
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5365 136
Size: 107 122 71 631 605 3693 1525
Fragments arranged by size:
3693 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3439 6749 3439
Size: 3310 3444
BlpI GC'TnA_GC
Cuts at: 4171 4171
Size: 6754
Bme1580I G_kGCm'C
Cuts at: 1790 2624 3865 4815 5971 1790
Size: 834 1241 950 1156 2573
Fragments arranged by size:
2573 1241 1156 950 834
BmgBI CAC'GTC
Cuts at: 1318 3172 1318
Size: 1854 4900
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3868 4485 5443
Size: 416 53 83 399 24 2451 617 958
Cuts at: 5443 442
Size: 1753
Fragments arranged by size:
2451 1753 958 617 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6754
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 3555 3566 4023 5414 1239
Size: 1184 1132 11 457 1391 2579
Fragments arranged by size:
2579 1391 1184 1132 457 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3850 4879 5744 6042 3850
Size: 1029 865 298 4562
Fragments arranged by size:
4562 1029 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2439 2939 3132 5417 882
Size: 617 940 500 193 2285 2219
Fragments arranged by size:
2285 2219 940 617 500 193
BsaAI yAC'GTr
Cuts at: 493 1204 2205 2535 4545 493
Size: 711 1001 330 2010 2702
Fragments arranged by size:
2702 2010 1001 711 330
BsaBI GATnn'nnATC
Cuts at: 2732 6748 2732
Size: 4016 2738
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 2343 4561 4943
Size: 53 83 186 936 740 70 2218 382
Cuts at: 4943 5578 275
Size: 635 1451
Fragments arranged by size:
2218 1451 936 740 635 382 186 83
70 53
BsaWI w'CCGG_w
Cuts at: 2448 5187 5585 5859 6006 6417 2448
Size: 2739 398 274 147 411 2785
Fragments arranged by size:
2785 2739 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6529
Fragments arranged by size:
6529 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2228 1186
Size: 1042 5712
BseYI C'CCAG_C
Cuts at: 3864 5957 6453 3864
Size: 2093 496 4165
Fragments arranged by size:
4165 2093 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2290 1163
Size: 573 554 5627
Fragments arranged by size:
5627 573 554
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2340 3286 4204 4965 5993 1540
Size: 275 186 339 946 918 761 1028 2301
Fragments arranged by size:
2301 1028 946 918 761 339 275 186
BsiHKAI G_wGCw'C
Cuts at: 2624 3373 3822 4815 4900 5971 2624
Size: 749 449 993 85 1071 3407
Fragments arranged by size:
3407 1071 993 749 449 85
BsmI GAATG_Cn'
Cuts at: 3203 4265 4358 3203
Size: 1062 93 5599
Fragments arranged by size:
5599 1062 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2264 2264
Size: 6754
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2624 3373 3822 3865 4073 4815 4900
Size: 399 834 749 449 43 208 742 85
Cuts at: 4900 5971 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 834 749 742 449 399 208
85 43
BspEI T'CCGG_A
Cuts at: 6417 6417
Size: 6754
BspHI T'CATG_A
Cuts at: 3768 4643 6373 3768
Size: 875 1730 4149
Fragments arranged by size:
4149 1730 875
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4109 844
Size: 3265 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4641 1394
Size: 429 2818 3507
Fragments arranged by size:
3507 2818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3529 3627 5249 5423 65
Size: 1183 2281 98 1622 174 1396
Fragments arranged by size:
2281 1622 1396 1183 174 98
BsrFI r'CCGG_y
Cuts at: 1700 1810 2339 5398 5585 1700
Size: 110 529 3059 187 2869
Fragments arranged by size:
3059 2869 529 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6754
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6754
BssSI C'ACGA_G
Cuts at: 2267 3366 3470 4814 5826 2267
Size: 1099 104 1344 1012 3195
Fragments arranged by size:
3195 1344 1099 1012 104
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6754
BstBI TT'CG_AA
Cuts at: 5558 5558
Size: 6754
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6754
BstXI CCAn_nnnn'nTGG
Cuts at: 2513 2920 3793 2513
Size: 407 873 5474
Fragments arranged by size:
5474 873 407
BstYI r'GATC_y
Cuts at: 1972 2371 2451 3219 3439 3576 3753 4084 4451
Size: 399 80 768 220 137 177 331 367
Cuts at: 4451 4836 4853 6305 6391 6403 6749 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 768 399 385 367 346 331
220 177 137 86 80 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3534 513
Size: 552 2469 3733
Fragments arranged by size:
3733 2469 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3860 487
Size: 683 56 2634 3381
Fragments arranged by size:
3381 2634 683 56
BtsI GCAGTG_nn'
Cuts at: 2067 2563 2795 4270 5064 5084 2067
Size: 496 232 1475 794 20 3737
Fragments arranged by size:
3737 1475 794 496 232 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6719
DraI TTT'AAA
Cuts at: 4058 4058
Size: 6754
DraIII CAC_nnn'GTG
Cuts at: 2296 2296
Size: 6754
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5761 809
Size: 507 4445 1802
Fragments arranged by size:
4445 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 2256 2298
Size: 54 1425 50 275 18 412 14 42
Cuts at: 2298 2529 3537 4201 5090 6719 8
Size: 231 1008 664 889 1629 43
Fragments arranged by size:
1629 1425 1008 889 664 412 275 231
54 50 43 42 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 4201 1537
Size: 275 2389 4090
Fragments arranged by size:
4090 2389 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2108 3951 4684 1353
Size: 755 1843 733 3423
Fragments arranged by size:
3423 1843 755 733
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5341 5715 5861 387
Size: 1064 138 3752 374 146 1280
Fragments arranged by size:
3752 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3371 3371
Size: 6754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2448 2810 3182 3555 3566 4023
Size: 393 791 25 362 372 373 11 457
Cuts at: 4023 4817 5414 6201 6469 1239
Size: 794 597 787 268 1524
Fragments arranged by size:
1524 794 791 787 597 457 393 373
372 362 268 25 11
EcoNI CCTnn'n_nnAGG
Cuts at: 3692 3806 3692
Size: 114 6640
EcoRI G'AATT_C
Cuts at: 4063 4441 4063
Size: 378 6376
FspI TGC'GCA
Cuts at: 2333 5260 2333
Size: 2927 3827
HaeII r_GCGC'y
Cuts at: 1678 2346 2479 5901 1678
Size: 668 133 3422 2531
Fragments arranged by size:
3422 2531 668 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2436 2468 3042 3074 3211
Size: 32 135 32 864 32 574 32 137
Cuts at: 3211 3243 3268 3300 3891 3909 3923 3941 5395
Size: 32 25 32 591 18 14 18 1454
Cuts at: 5395 5427 5469 5501 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1454 864 591 574 137 135 42
32 32 32 32 32 32 32 32
25 18 18 14
HincII GTy'rAC
Cuts at: 669 1657 1938 4098 4344 669
Size: 988 281 2160 246 3079
Fragments arranged by size:
3079 2160 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6754
HpaI GTT'AAC
Cuts at: 4344 4344
Size: 6754
KasI G'GCGC_C
Cuts at: 2342 2342
Size: 6754
KpnI G_GTAC'C
Cuts at: 4079 4079
Size: 6754
MfeI C'AATT_G
Cuts at: 3975 4353 3975
Size: 378 6376
MluI A'CGCG_T
Cuts at: 6630 6630
Size: 6754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2962 2981 3069 3444 5868 6052 1305
Size: 1657 19 88 375 2424 184 2007
Fragments arranged by size:
2424 2007 1657 375 184 88 19
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2300 2531 10
Size: 54 1425 343 468 231 4233
Fragments arranged by size:
4233 1425 468 343 231 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3080 3741 4712 5071 5230 518
Size: 648 1914 661 971 359 159 2042
Fragments arranged by size:
2042 1914 971 661 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2082 2122 3868 4170 4847 5995 6240 1606
Size: 476 40 1746 302 677 1148 245 2120
Fragments arranged by size:
2120 1746 1148 677 476 302 245 40
NarI GG'CG_CC
Cuts at: 2343 2343
Size: 6754
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6202
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6754
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6754
NotI GC'GGCC_GC
Cuts at: 4201 4201
Size: 6754
NspI r_CATG'y
Cuts at: 1132 1229 4112 5657 1132
Size: 97 2883 1545 2229
Fragments arranged by size:
2883 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2278 1348
Size: 930 5824
PciI A'CATG_T
Cuts at: 5653 5653
Size: 6754
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2082 2125 2296 1108
Size: 974 43 171 5566
Fragments arranged by size:
5566 974 171 43
PfoI T'CCnGG_A
Cuts at: 2319 3582 2319
Size: 1263 5491
PmeI GTTT'AAAC
Cuts at: 4058 4058
Size: 6754
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4823 4855 6336 6368 4823
Size: 32 1481 32 5209
Fragments arranged by size:
5209 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6754
PsiI TTA'TAA
Cuts at: 4324 4324
Size: 6754
PspOMI G'GGCC_C
Cuts at: 3861 3861
Size: 6754
PspXI vC'TCGA_Gb
Cuts at: 2249 2249
Size: 6754
PstI C_TGCA'G
Cuts at: 830 2073 2316 2570 3183 3388 3607 4106 830
Size: 1243 243 254 613 205 219 499 3478
Fragments arranged by size:
3478 1243 613 499 254 243 219 205
PvuI CG_AT'CG
Cuts at: 2001 3286 2001
Size: 1285 5469
PvuII CAG'CTG
Cuts at: 2122 3868 2122
Size: 1746 5008
SacI G_AGCT'C
Cuts at: 3373 3373
Size: 6754
SalI G'TCGA_C
Cuts at: 1936 4096 1936
Size: 2160 4594
SapI GCTCTTCn'nnn_
Cuts at: 2108 3951 2108
Size: 1843 4911
SbfI CC_TGCA'GG
Cuts at: 4106 4106
Size: 6754
ScaI AGT'ACT
Cuts at: 5002 6714 5002
Size: 1712 5042
SexAI A'CCwGG_T
Cuts at: 2154 2154
Size: 6754
SfcI C'TryA_G
Cuts at: 826 1045 2069 2312 2566 3179 3384 3433 3603
Size: 219 1024 243 254 613 205 49 170
Cuts at: 3603 4102 5237 5918 6109 826
Size: 499 1135 681 191 1471
Fragments arranged by size:
1471 1135 1024 681 613 499 254 243
219 205 191 170 49
SfoI GGC'GCC
Cuts at: 2344 2344
Size: 6754
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6754
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6754
SmaI CCC'GGG
Cuts at: 1872 4081 1872
Size: 2209 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 2375 3829 4858 5759 6021
Size: 197 926 306 126 1454 1029 901 262
Cuts at: 6021 820
Size: 1553
Fragments arranged by size:
1553 1454 1029 926 901 306 262 197
126
SnaBI TAC'GTA
Cuts at: 493 4545 493
Size: 4052 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6754
SphI G_CATG'C
Cuts at: 1132 4112 1132
Size: 2980 3774
SspI AAT'ATT
Cuts at: 5 52 2686 2950 4678 5
Size: 47 2634 264 1728 2081
Fragments arranged by size:
2634 2081 1728 264 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6754
StyI C'CwwG_G
Cuts at: 513 1065 4193 513
Size: 552 3128 3074
Fragments arranged by size:
3128 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2356 3224 4781 4798 4951 5136 5581
Size: 1255 776 868 1557 17 153 185 445
Cuts at: 5581 325
Size: 1498
Fragments arranged by size:
1557 1498 1255 868 776 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2669 5000 6712
Size: 274 80 33 51 1443 692 2331 1712
Cuts at: 6712 96
Size: 138
Fragments arranged by size:
2331 1712 1443 692 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6714 6746 6714
Size: 32 6722
Tth111I GACn'n_nGTC
Cuts at: 1316 3455 1316
Size: 2139 4615
XbaI T'CTAG_A
Cuts at: 4090 4090
Size: 6754
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2510 3790 2510
Size: 1280 5474
XhoI C'TCGA_G
Cuts at: 1943 2249 2375 1943
Size: 306 126 6322
Fragments arranged by size:
6322 306 126
XmaI C'CCGG_G
Cuts at: 1870 4079 1870
Size: 2209 4545
XmnI GAAnn'nnTTC
Cuts at: 2950 4024 4883 2950
Size: 1074 859 4821
Fragments arranged by size:
4821 1074 859
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4562 276
Size: 53 83 186 936 3028 2468
Fragments arranged by size:
3028 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BstZ17I Bsu36I EcoO109I EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PpuMI PsrI RsrII SacII SanDI SfiI SrfI SwaI