Restriction map for
pFN21AB9425
(Circular) (Six-base) MAPSORT of: pFN21AB9425.seq Check: 2424 from: 1 to: 6916
pFN21AB9425 6916 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2627 2627
Size: 6916
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3333 4089 4726 278
Size: 53 83 186 936 1797 756 637 2468
Fragments arranged by size:
2468 1797 936 756 637 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4259 1937
Size: 2322 4594
Acc65I G'GTAC_C
Cuts at: 4237 4237
Size: 6916
AclI AA'CG_TT
Cuts at: 1563 5043 5416 1563
Size: 3480 373 3063
Fragments arranged by size:
3480 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3323 4979 6363 6631 1632
Size: 1691 1656 1384 268 1917
Fragments arranged by size:
1917 1691 1656 1384 268
AfeI AGC'GCT
Cuts at: 3503 3503
Size: 6916
AflII C'TTAA_G
Cuts at: 820 1017 2027 820
Size: 197 1010 5709
Fragments arranged by size:
5709 1010 197
AflIII A'CryG_T
Cuts at: 1203 5815 6792 1203
Size: 4612 977 1327
Fragments arranged by size:
4612 1327 977
AgeI A'CCGG_T
Cuts at: 5747 5747
Size: 6916
AhdI GACnn_n'nnGTC
Cuts at: 5645 5645
Size: 6916
AleI CACnn'nnGTG
Cuts at: 1166 2219 2621 4136 1166
Size: 1053 402 1515 3946
Fragments arranged by size:
3946 1515 1053 402
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6916
AlwNI CAG_nnn'CTG
Cuts at: 4659 6231 4659
Size: 1572 5344
ApaI G_GGCC'C
Cuts at: 3781 3781
Size: 6916
ApaLI G'TGCA_C
Cuts at: 4973 6129 4973
Size: 1156 5760
ApoI r'AATT_y
Cuts at: 1493 3512 4175 4225 4451 4603 6712 1493
Size: 2019 663 50 226 152 2109 1697
Fragments arranged by size:
2109 2019 1697 663 226 152 50
AseI AT'TA_AT
Cuts at: 160 1032 5470 160
Size: 872 4438 1606
Fragments arranged by size:
4438 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
AvaI C'yCGr_G
Cuts at: 1870 1943 4113 4241 1870
Size: 73 2170 128 4545
Fragments arranged by size:
4545 2170 128 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2590 2623 3389 3422 2590
Size: 33 766 33 6084
Fragments arranged by size:
6084 766 33 33
BamHI G'GATC_C
Cuts at: 4246 4613 4246
Size: 367 6549
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2637 2981 4237 5692 618
Size: 325 217 627 850 344 1256 1455 1842
Fragments arranged by size:
1842 1455 1256 850 627 344 325 217
BanII G_rGCy'C
Cuts at: 1391 2649 2705 3170 3781 4235 1391
Size: 1258 56 465 611 454 4072
Fragments arranged by size:
4072 1258 611 465 454 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2186 4209 928
Size: 971 287 2023 3635
Fragments arranged by size:
3635 2023 971 287
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5107 5141 1903
Size: 34 3170 34 3678
Fragments arranged by size:
3678 3170 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2591 2753 4808 6018 2591
Size: 162 2055 1210 3489
Fragments arranged by size:
3489 2055 1210 162
BclI T'GATC_A
Cuts at: 1548 2385 1548
Size: 837 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5527 136
Size: 107 122 71 631 605 3855 1525
Fragments arranged by size:
3855 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6911 6911
Size: 6916
BlpI GC'TnA_GC
Cuts at: 3893 4333 3893
Size: 440 6476
Bme1580I G_kGCm'C
Cuts at: 1790 3781 4977 6133 1790
Size: 1991 1196 1156 2573
Fragments arranged by size:
2573 1991 1196 1156
BmgBI CAC'GTC
Cuts at: 1318 3352 3874 1318
Size: 2034 522 4360
Fragments arranged by size:
4360 2034 522
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3878 4030 4647
Size: 416 53 83 399 24 2461 152 617
Cuts at: 4647 5605 442
Size: 958 1753
Fragments arranged by size:
2461 1753 958 617 416 399 152 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6916
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 4052 5576 1239
Size: 1142 1671 1524 2579
Fragments arranged by size:
2579 1671 1524 1142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3269 5041 5906 6204 3101
Size: 168 1772 865 298 3813
Fragments arranged by size:
3813 1772 865 298 168
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2678 2852 3474 5579 882
Size: 617 1179 174 622 2105 2219
Fragments arranged by size:
2219 2105 1179 622 617 174
BsaAI yAC'GTr
Cuts at: 493 1204 4707 493
Size: 711 3503 2702
Fragments arranged by size:
3503 2702 711
BsaBI GATnn'nnATC
Cuts at: 2036 6910 2036
Size: 4874 2042
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3330 3984 4086 4723
Size: 53 83 186 936 1797 654 102 637
Cuts at: 4723 5105 5740 275
Size: 382 635 1451
Fragments arranged by size:
1797 1451 936 654 637 635 382 186
102 83 53
BsaWI w'CCGG_w
Cuts at: 5349 5747 6021 6168 6579 5349
Size: 398 274 147 411 5686
Fragments arranged by size:
5686 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3737 3767 1178
Size: 30 165 30 2334 30 4327
Fragments arranged by size:
4327 2334 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2327 3135 3140 1186
Size: 1141 808 5 4962
Fragments arranged by size:
4962 1141 808 5
BseYI C'CCAG_C
Cuts at: 2666 2706 6119 6615 2666
Size: 40 3413 496 2967
Fragments arranged by size:
3413 2967 496 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2281 2599 3343 1163
Size: 573 545 318 744 4736
Fragments arranged by size:
4736 744 573 545 318
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2102 2462 4366 5127 6155 1540
Size: 275 186 101 360 1904 761 1028 2301
Fragments arranged by size:
2301 1904 1028 761 360 275 186 101
BsiHKAI G_wGCw'C
Cuts at: 2649 3132 4029 4977 5062 6133 2649
Size: 483 897 948 85 1071 3432
Fragments arranged by size:
3432 1071 948 897 483 85
BsmI GAATG_Cn'
Cuts at: 4427 4520 4427
Size: 93 6823
BsmBI CGTCTCn'nnnn_
Cuts at: 3150 4077 3150
Size: 927 5989
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2649 2705 3132 3170 3781 4029 4235
Size: 399 859 56 427 38 611 248 206
Cuts at: 4235 4977 5062 6133 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 859 742 611 427 399 248
206 85 56 38
BspEI T'CCGG_A
Cuts at: 6579 6579
Size: 6916
BspHI T'CATG_A
Cuts at: 3927 4805 6535 3927
Size: 878 1730 4308
Fragments arranged by size:
4308 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2627 4271 844
Size: 1783 1644 3489
Fragments arranged by size:
3489 1783 1644
BsrBI CCG'CTC
Cuts at: 1394 1823 4803 1394
Size: 429 2980 3507
Fragments arranged by size:
3507 2980 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 2557 2973 5411 5585 65
Size: 1183 879 430 416 2438 174 1396
Fragments arranged by size:
2438 1396 1183 879 430 416 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4123 5560 5747 1700
Size: 110 2313 1437 187 2869
Fragments arranged by size:
2869 2313 1437 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6916
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6916
BssSI C'ACGA_G
Cuts at: 2642 4976 5988 2642
Size: 2334 1012 3570
Fragments arranged by size:
3570 2334 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2815 794
Size: 2021 4895
BstBI TT'CG_AA
Cuts at: 5720 5720
Size: 6916
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6916
BstXI CCAn_nnnn'nTGG
Cuts at: 2911 3211 3581 2911
Size: 300 370 6246
Fragments arranged by size:
6246 370 300
BstYI r'GATC_y
Cuts at: 1972 3735 4246 4613 4998 5015 6467 6553 6565
Size: 1763 511 367 385 17 1452 86 12
Cuts at: 6565 6911 1972
Size: 346 1977
Fragments arranged by size:
1977 1763 1452 511 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3765 513
Size: 552 939 1761 3664
Fragments arranged by size:
3664 1761 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3951 487
Size: 683 56 2725 3452
Fragments arranged by size:
3452 2725 683 56
BtsI GCAGTG_nn'
Cuts at: 2825 3637 4432 5226 5246 2825
Size: 812 795 794 20 4495
Fragments arranged by size:
4495 812 795 794 20
ClaI AT'CG_AT
Cuts at: 1554 2391 3031 1554
Size: 837 640 5439
Fragments arranged by size:
5439 837 640
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6881
DraI TTT'AAA
Cuts at: 4220 4220
Size: 6916
DraIII CAC_nnn'GTG
Cuts at: 2661 2661
Size: 6916
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2210 5923 809
Size: 507 894 3713 1802
Fragments arranged by size:
3713 1802 894 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2099 2158 2166
Size: 54 1425 50 275 18 269 59 8
Cuts at: 2166 2289 2459 2509 2523 2913 2953 3154 4363
Size: 123 170 50 14 390 40 201 1209
Cuts at: 4363 5252 6881 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1209 889 390 275 269 201
170 123 59 54 50 50 43 40
18 14 8
EagI C'GGCC_G
Cuts at: 1537 1812 2099 2459 4363 1537
Size: 275 287 360 1904 4090
Fragments arranged by size:
4090 1904 360 287 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2368 2427 3368 4113 4846 1353
Size: 1015 59 941 745 733 3423
Fragments arranged by size:
3423 1015 941 745 733 59
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5503 5877 6023 387
Size: 1064 138 3914 374 146 1280
Fragments arranged by size:
3914 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2647 2647
Size: 6916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 3323 4052 4979 5576 6363 6631
Size: 393 749 942 729 927 597 787 268
Cuts at: 6631 1239
Size: 1524
Fragments arranged by size:
1524 942 927 787 749 729 597 393
268
EcoO109I rG'GnC_Cy
Cuts at: 2093 2758 2784 3778 2093
Size: 665 26 994 5231
Fragments arranged by size:
5231 994 665 26
EcoRI G'AATT_C
Cuts at: 4175 4225 4603 4175
Size: 50 378 6488
Fragments arranged by size:
6488 378 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4072 4072
Size: 6916
FspI TGC'GCA
Cuts at: 5422 5422
Size: 6916
HaeII r_GCGC'y
Cuts at: 1678 3211 3505 3952 6063 1678
Size: 1533 294 447 2111 2531
Fragments arranged by size:
2531 2111 1533 447 294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2223 2255 2377 2409 2556
Size: 32 135 32 651 32 122 32 147
Cuts at: 2556 2588 3370 3402 3539 3571 3922 3954 5557
Size: 32 782 32 137 32 351 32 1603
Cuts at: 5557 5589 5631 5663 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1603 782 651 351 147 137 135
122 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2506 2722 4260 4506 669
Size: 988 281 568 216 1538 246 3079
Fragments arranged by size:
3079 1538 988 568 281 246 216
HindIII A'AGCT_T
Cuts at: 748 3921 748
Size: 3173 3743
HpaI GTT'AAC
Cuts at: 4506 4506
Size: 6916
KpnI G_GTAC'C
Cuts at: 4241 4241
Size: 6916
MfeI C'AATT_G
Cuts at: 4149 4515 4149
Size: 366 6550
MluI A'CGCG_T
Cuts at: 6792 6792
Size: 6916
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3729 6030 6214 1305
Size: 2424 2301 184 2007
Fragments arranged by size:
2424 2301 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 2525 3156 10
Size: 54 1425 343 459 234 631 3770
Fragments arranged by size:
3770 1425 631 459 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2219 2621 3239 3579 4136 4874
Size: 648 843 210 402 618 340 557 738
Cuts at: 4874 5233 5392 518
Size: 359 159 2042
Fragments arranged by size:
2042 843 738 648 618 557 402 359
340 210 159
MspA1I CmG'CkG
Cuts at: 1606 2710 2952 3160 4332 5009 6157 6402 1606
Size: 1104 242 208 1172 677 1148 245 2120
Fragments arranged by size:
2120 1172 1148 1104 677 245 242 208
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5425
Fragments arranged by size:
5425 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6916
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6916
NotI GC'GGCC_GC
Cuts at: 4363 4363
Size: 6916
NspI r_CATG'y
Cuts at: 1132 1229 2458 2728 4274 5819 1132
Size: 97 1229 270 1546 1545 2229
Fragments arranged by size:
2229 1546 1545 1229 270 97
PasI CC'CwG_GG
Cuts at: 1348 3781 1348
Size: 2433 4483
PciI A'CATG_T
Cuts at: 5815 5815
Size: 6916
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2070 2089 1108
Size: 962 19 5935
Fragments arranged by size:
5935 962 19
PfoI T'CCnGG_A
Cuts at: 4029 4029
Size: 6916
PmeI GTTT'AAAC
Cuts at: 4220 4220
Size: 6916
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2426 2458 4985 5017 6498 6530 2426
Size: 32 2527 32 1481 32 2812
Fragments arranged by size:
2812 2527 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2093 2758 2784 2093
Size: 665 26 6225
Fragments arranged by size:
6225 665 26
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6916
PsiI TTA'TAA
Cuts at: 4486 4486
Size: 6916
PspOMI G'GGCC_C
Cuts at: 3777 3777
Size: 6916
PstI C_TGCA'G
Cuts at: 830 2325 3547 3744 4268 830
Size: 1495 1222 197 524 3478
Fragments arranged by size:
3478 1495 1222 524 197
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6916
PvuII CAG'CTG
Cuts at: 3160 3160
Size: 6916
SacI G_AGCT'C
Cuts at: 2649 2649
Size: 6916
SalI G'TCGA_C
Cuts at: 1936 4258 1936
Size: 2322 4594
SanDI GG'GwC_CC
Cuts at: 2093 2784 2093
Size: 691 6225
SapI GCTCTTCn'nnn_
Cuts at: 2427 4113 2427
Size: 1686 5230
SbfI CC_TGCA'GG
Cuts at: 3744 4268 3744
Size: 524 6392
ScaI AGT'ACT
Cuts at: 2910 5164 6876 2910
Size: 2254 1712 2950
Fragments arranged by size:
2950 2254 1712
SexAI A'CCwGG_T
Cuts at: 2694 2694
Size: 6916
SfcI C'TryA_G
Cuts at: 826 1045 2321 3543 3592 3740 4264 5399 6080
Size: 219 1276 1222 49 148 524 1135 681
Cuts at: 6080 6271 826
Size: 191 1471
Fragments arranged by size:
1471 1276 1222 1135 681 524 219 191
148 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6916
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6916
SmaI CCC'GGG
Cuts at: 1872 4115 4243 1872
Size: 2243 128 4545
Fragments arranged by size:
4545 2243 128
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 3080 3284 5020 5921 6183
Size: 197 926 84 1053 204 1736 901 262
Cuts at: 6183 820
Size: 1553
Fragments arranged by size:
1736 1553 1053 926 901 262 204 197
84
SnaBI TAC'GTA
Cuts at: 493 4707 493
Size: 4214 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6916
SphI G_CATG'C
Cuts at: 1132 2458 4274 1132
Size: 1326 1816 3774
Fragments arranged by size:
3774 1816 1326
SspI AAT'ATT
Cuts at: 5 52 4840 5
Size: 47 4788 2081
Fragments arranged by size:
4788 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6916
StyI C'CwwG_G
Cuts at: 513 1065 2004 3438 4355 513
Size: 552 939 1434 917 3074
Fragments arranged by size:
3074 1434 939 917 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3061 4012 4014 4943 4960 5113 5298
Size: 1255 1481 951 2 929 17 153 185
Cuts at: 5298 5743 325
Size: 445 1498
Fragments arranged by size:
1498 1481 1255 951 929 445 185 153
17 2
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2908 5162 6874
Size: 274 80 33 51 1443 931 2254 1712
Cuts at: 6874 96
Size: 138
Fragments arranged by size:
2254 1712 1443 931 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2768 2800 6876 6908 2768
Size: 32 4076 32 2776
Fragments arranged by size:
4076 2776 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6916
XbaI T'CTAG_A
Cuts at: 4252 4252
Size: 6916
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2881 2881
Size: 6916
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6916
XmaI C'CCGG_G
Cuts at: 1870 4113 4241 1870
Size: 2243 128 4545
Fragments arranged by size:
4545 2243 128
XmnI GAAnn'nnTTC
Cuts at: 3675 3923 5045 3675
Size: 248 1122 5546
Fragments arranged by size:
5546 1122 248
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3331 4087 4724 276
Size: 53 83 186 936 1797 756 637 2468
Fragments arranged by size:
2468 1797 936 756 637 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI RsrII SacII SfiI SfoI SrfI SwaI