Restriction map for
pFN21AB9423
(Circular) (Six-base) MAPSORT of: pFN21AB9423.seq Check: 12 from: 1 to: 5356
pFN21AB9423 5356 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2567 2567
Size: 5356
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3166 278
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2502 2699 1937
Size: 565 197 4594
Fragments arranged by size:
4594 565 197
Acc65I G'GTAC_C
Cuts at: 2677 2677
Size: 5356
AclI AA'CG_TT
Cuts at: 1563 2421 3483 3856 1563
Size: 858 1062 373 3063
Fragments arranged by size:
3063 1062 858 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 3419 4803 5071 1632
Size: 410 1377 1384 268 1917
Fragments arranged by size:
1917 1384 1377 410 268
AfeI AGC'GCT
Cuts at: 2087 2087
Size: 5356
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5159
AflIII A'CryG_T
Cuts at: 1203 4255 5232 1203
Size: 3052 977 1327
Fragments arranged by size:
3052 1327 977
AgeI A'CCGG_T
Cuts at: 4187 4187
Size: 5356
AhdI GACnn_n'nnGTC
Cuts at: 4085 4085
Size: 5356
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5356
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5356
AlwNI CAG_nnn'CTG
Cuts at: 3099 4671 3099
Size: 1572 3784
ApaI G_GGCC'C
Cuts at: 2288 2288
Size: 5356
ApaLI G'TGCA_C
Cuts at: 2495 3413 4569 2495
Size: 918 1156 3282
Fragments arranged by size:
3282 1156 918
ApoI r'AATT_y
Cuts at: 1493 2665 2891 3043 5152 1493
Size: 1172 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3910 160
Size: 872 2878 1606
Fragments arranged by size:
2878 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
AvaI C'yCGr_G
Cuts at: 1870 1943 2161 2681 1870
Size: 73 218 520 4545
Fragments arranged by size:
4545 520 218 73
BamHI G'GATC_C
Cuts at: 2686 3053 2686
Size: 367 4989
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 2234 2677 4132 618
Size: 325 217 627 291 156 443 1455 1842
Fragments arranged by size:
1842 1455 627 443 325 291 217 156
BanII G_rGCy'C
Cuts at: 1391 2288 2540 2675 1391
Size: 897 252 135 4072
Fragments arranged by size:
4072 897 252 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2023 928
Size: 971 124 4261
Fragments arranged by size:
4261 971 124
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3547 3581 1903
Size: 34 1610 34 3678
Fragments arranged by size:
3678 1610 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3248 4458 3248
Size: 1210 4146
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5356
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3967 136
Size: 107 122 71 631 605 2295 1525
Fragments arranged by size:
2295 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5351 5351
Size: 5356
BlpI GC'TnA_GC
Cuts at: 2773 2773
Size: 5356
Bme1580I G_kGCm'C
Cuts at: 1790 2081 2288 2499 3417 4573 1790
Size: 291 207 211 918 1156 2573
Fragments arranged by size:
2573 1156 918 291 211 207
BmgBI CAC'GTC
Cuts at: 1318 2500 1318
Size: 1182 4174
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2191 3087 4045
Size: 416 53 83 399 24 774 896 958
Cuts at: 4045 442
Size: 1753
Fragments arranged by size:
1753 958 896 774 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2639 4016 1239
Size: 1400 1377 2579
Fragments arranged by size:
2579 1400 1377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3481 4346 4644 3481
Size: 865 298 4193
Fragments arranged by size:
4193 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4019 882
Size: 617 2520 2219
Fragments arranged by size:
2520 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2197 2551 3147 493
Size: 711 993 354 596 2702
Fragments arranged by size:
2702 993 711 596 354
BsaBI GATnn'nnATC
Cuts at: 5350 5350
Size: 5356
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2114 3163 3545 4180
Size: 53 83 186 936 581 1049 382 635
Cuts at: 4180 275
Size: 1451
Fragments arranged by size:
1451 1049 936 635 581 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2325 3789 4187 4461 4608 5019 2325
Size: 1464 398 274 147 411 2662
Fragments arranged by size:
2662 1464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5131
Fragments arranged by size:
5131 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5356
BseYI C'CCAG_C
Cuts at: 2082 4559 5055 2082
Size: 2477 496 2383
Fragments arranged by size:
2477 2383 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2171 2236 1163
Size: 573 345 90 65 4283
Fragments arranged by size:
4283 573 345 90 65
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2806 3567 4595 1540
Size: 275 186 805 761 1028 2301
Fragments arranged by size:
2301 1028 805 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2499 3417 3502 4573 2499
Size: 918 85 1071 3282
Fragments arranged by size:
3282 1071 918 85
BsmI GAATG_Cn'
Cuts at: 2867 2960 2867
Size: 93 5263
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2288 2499 2540 2675 3417 3502
Size: 399 291 207 211 41 135 742 85
Cuts at: 3502 4573 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 291 211 207 135
85 41
BspEI T'CCGG_A
Cuts at: 2325 5019 2325
Size: 2694 2662
BspHI T'CATG_A
Cuts at: 3245 4975 3245
Size: 1730 3626
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 2373 2567 2711 844
Size: 1301 228 194 144 3489
Fragments arranged by size:
3489 1301 228 194 144
BsrBI CCG'CTC
Cuts at: 1394 1823 3243 1394
Size: 429 1420 3507
Fragments arranged by size:
3507 1420 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3851 4025 65
Size: 1183 2603 174 1396
Fragments arranged by size:
2603 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4000 4187 1700
Size: 110 2190 187 2869
Fragments arranged by size:
2869 2190 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5356
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5356
BssSI C'ACGA_G
Cuts at: 3416 4428 3416
Size: 1012 4344
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2458 794
Size: 1664 3692
BstBI TT'CG_AA
Cuts at: 4160 4160
Size: 5356
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5356
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2617 2359
Size: 258 5098
BstYI r'GATC_y
Cuts at: 1972 2466 2686 3053 3438 3455 4907 4993 5005
Size: 494 220 367 385 17 1452 86 12
Cuts at: 5005 5351 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 494 385 367 346 220 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2334 513
Size: 552 1269 3535
Fragments arranged by size:
3535 1269 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2509 487
Size: 683 56 1283 3334
Fragments arranged by size:
3334 1283 683 56
BtsI GCAGTG_nn'
Cuts at: 2872 3666 3686 2872
Size: 794 20 4542
Fragments arranged by size:
4542 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5356
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2404 2439 529
Size: 35 1840 35 3446
Fragments arranged by size:
3446 1840 35 35
DraI TTT'AAA
Cuts at: 2660 2660
Size: 5356
DraIII CAC_nnn'GTG
Cuts at: 2200 2200
Size: 5356
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4363 809
Size: 507 3047 1802
Fragments arranged by size:
3047 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2803 3692
Size: 54 1425 50 275 18 315 658 889
Cuts at: 3692 5321 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 658 315 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2803 1537
Size: 275 991 4090
Fragments arranged by size:
4090 991 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2414 3286 1353
Size: 1061 872 3423
Fragments arranged by size:
3423 1061 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2042 3943 4317 4463 387
Size: 1064 138 453 1901 374 146 1280
Fragments arranged by size:
1901 1280 1064 453 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2639 3419 4016 4803 5071 1239
Size: 393 410 597 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 597 597 410 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2170 2284 2285 2404 2170
Size: 114 1 119 5122
Fragments arranged by size:
5122 119 114 1
EcoRI G'AATT_C
Cuts at: 2665 3043 2665
Size: 378 4978
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 5356
FspI TGC'GCA
Cuts at: 3862 3862
Size: 5356
HaeII r_GCGC'y
Cuts at: 1678 2089 4503 1678
Size: 411 2414 2531
Fragments arranged by size:
2531 2414 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373
Size: 32 135 32 2425 32 42 32 2626
Fragments arranged by size:
2626 2425 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2221 2700 2946 669
Size: 988 281 283 479 246 3079
Fragments arranged by size:
3079 988 479 283 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5356
HpaI GTT'AAC
Cuts at: 2946 2946
Size: 5356
KpnI G_GTAC'C
Cuts at: 2681 2681
Size: 5356
MfeI C'AATT_G
Cuts at: 2955 2955
Size: 5356
MluI A'CGCG_T
Cuts at: 5232 5232
Size: 5356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2034 4470 4654 1305
Size: 729 2436 184 2007
Fragments arranged by size:
2436 2007 729 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3534
Fragments arranged by size:
3534 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3314 3673 3832 518
Size: 648 2148 359 159 2042
Fragments arranged by size:
2148 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2155 2304 2772 3449 4597 4842 1606
Size: 549 149 468 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 549 468 245 149
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4804
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5356
NotI GC'GGCC_GC
Cuts at: 2803 2803
Size: 5356
NspI r_CATG'y
Cuts at: 1132 1229 2714 4259 1132
Size: 97 1485 1545 2229
Fragments arranged by size:
2229 1545 1485 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5356
PciI A'CATG_T
Cuts at: 4255 4255
Size: 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5356
PmeI GTTT'AAAC
Cuts at: 2660 2660
Size: 5356
PmlI CAC'GTG
Cuts at: 2197 2551 2197
Size: 354 5002
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2306 2338 3425 3457 4938 4970 2306
Size: 32 1087 32 1481 32 2692
Fragments arranged by size:
2692 1481 1087 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 2404 2170
Size: 234 5122
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5356
PsiI TTA'TAA
Cuts at: 2926 2926
Size: 5356
PspOMI G'GGCC_C
Cuts at: 2284 2284
Size: 5356
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2546 2578 2546
Size: 32 5324
PstI C_TGCA'G
Cuts at: 830 2454 2708 830
Size: 1624 254 3478
Fragments arranged by size:
3478 1624 254
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5356
PvuII CAG'CTG
Cuts at: 2155 2155
Size: 5356
SalI G'TCGA_C
Cuts at: 1936 2698 1936
Size: 762 4594
SbfI CC_TGCA'GG
Cuts at: 2708 2708
Size: 5356
ScaI AGT'ACT
Cuts at: 3604 5316 3604
Size: 1712 3644
SfcI C'TryA_G
Cuts at: 826 1045 2450 2704 3839 4520 4711 826
Size: 219 1405 254 1135 681 191 1471
Fragments arranged by size:
1471 1405 1135 681 254 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5356
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5356
SmaI CCC'GGG
Cuts at: 1872 2163 2683 1872
Size: 291 520 4545
Fragments arranged by size:
4545 520 291
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3460 4361 4623 820
Size: 197 926 1517 901 262 1553
Fragments arranged by size:
1553 1517 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3147 493
Size: 2654 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5356
SphI G_CATG'C
Cuts at: 1132 2714 1132
Size: 1582 3774
SspI AAT'ATT
Cuts at: 5 52 3280 5
Size: 47 3228 2081
Fragments arranged by size:
3228 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5356
StyI C'CwwG_G
Cuts at: 513 1065 2610 2795 513
Size: 552 1545 185 3074
Fragments arranged by size:
3074 1545 552 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2096 2173 3383 3400 3553 3738 4183
Size: 1255 516 77 1210 17 153 185 445
Cuts at: 4183 325
Size: 1498
Fragments arranged by size:
1498 1255 1210 516 445 185 153 77
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3602 5314 96
Size: 274 80 33 51 1443 1625 1712 138
Fragments arranged by size:
1712 1625 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2419 2451 5316 5348 2419
Size: 32 2865 32 2427
Fragments arranged by size:
2865 2427 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5356
XbaI T'CTAG_A
Cuts at: 2692 2692
Size: 5356
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5356
XmaI C'CCGG_G
Cuts at: 1870 2161 2681 1870
Size: 291 520 4545
Fragments arranged by size:
4545 520 291
XmnI GAAnn'nnTTC
Cuts at: 3485 3485
Size: 5356
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3164 276
Size: 53 83 186 936 1630 2468
Fragments arranged by size:
2468 1630 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI