Restriction map for
pFN21AB9422
(Circular) (Six-base) MAPSORT of: pFN21AB9422.seq Check: 2950 from: 1 to: 6418
pFN21AB9422 6418 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4228 278
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3761 1937
Size: 1824 4594
Acc65I G'GTAC_C
Cuts at: 3739 3739
Size: 6418
AclI AA'CG_TT
Cuts at: 1563 4545 4918 1563
Size: 2982 373 3063
Fragments arranged by size:
3063 2982 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 3275 4481 5865 6133 1632
Size: 945 698 1206 1384 268 1917
Fragments arranged by size:
1917 1384 1206 945 698 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6221
AflIII A'CryG_T
Cuts at: 1203 2103 3684 5317 6294 1203
Size: 900 1581 1633 977 1327
Fragments arranged by size:
1633 1581 1327 977 900
AgeI A'CCGG_T
Cuts at: 5249 5249
Size: 6418
AhdI GACnn_n'nnGTC
Cuts at: 5147 5147
Size: 6418
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6418
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3354 1184
Size: 2170 4248
AlwNI CAG_nnn'CTG
Cuts at: 2695 3615 4161 5733 2695
Size: 920 546 1572 3380
Fragments arranged by size:
3380 1572 920 546
ApaI G_GGCC'C
Cuts at: 2607 2607
Size: 6418
ApaLI G'TGCA_C
Cuts at: 4475 5631 4475
Size: 1156 5262
ApoI r'AATT_y
Cuts at: 1493 3007 3438 3727 3953 4105 6214 1493
Size: 1514 431 289 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 431 289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4972 160
Size: 872 3940 1606
Fragments arranged by size:
3940 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
AvaI C'yCGr_G
Cuts at: 1870 1943 3743 1870
Size: 73 1800 4545
Fragments arranged by size:
4545 1800 73
BamHI G'GATC_C
Cuts at: 3748 4115 3748
Size: 367 6051
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3739 5194 618
Size: 325 217 627 1952 1455 1842
Fragments arranged by size:
1952 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2145 2607 3385 3737 1391
Size: 754 462 778 352 4072
Fragments arranged by size:
4072 778 754 462 352
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3513 928
Size: 971 1614 3833
Fragments arranged by size:
3833 1614 971
BbvCI CC'TCA_GC
Cuts at: 3603 3603
Size: 6418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4609 4643 1903
Size: 34 2672 34 3678
Fragments arranged by size:
3678 2672 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4310 5520 4310
Size: 1210 5208
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6418
BfrBI ATG'CAT
Cuts at: 3194 3194
Size: 6418
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5029 136
Size: 107 122 71 631 605 3357 1525
Fragments arranged by size:
3357 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2636 6413 2636
Size: 3777 2641
BlpI GC'TnA_GC
Cuts at: 2955 3835 2955
Size: 880 5538
Bme1580I G_kGCm'C
Cuts at: 1790 2607 4479 5635 1790
Size: 817 1872 1156 2573
Fragments arranged by size:
2573 1872 1156 817
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6418
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 4149 5107
Size: 416 53 83 399 24 629 2103 958
Cuts at: 5107 442
Size: 1753
Fragments arranged by size:
2103 1753 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6418
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2374 2497 2882 5078 1239
Size: 1135 123 385 2196 2579
Fragments arranged by size:
2579 2196 1135 385 123
Bpu10I CC'TnA_GC
Cuts at: 2432 3603 2432
Size: 1171 5247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2323 2486 2732 4543 5408 5706 2323
Size: 163 246 1811 865 298 3035
Fragments arranged by size:
3035 1811 865 298 246 163
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5081 882
Size: 617 3582 2219
Fragments arranged by size:
3582 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3700 4209 493
Size: 711 2496 509 2702
Fragments arranged by size:
2702 2496 711 509
BsaBI GATnn'nnATC
Cuts at: 6412 6412
Size: 6418
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4225 4607 5242 275
Size: 53 83 186 936 2692 382 635 1451
Fragments arranged by size:
2692 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4851 5249 5523 5670 6081 4851
Size: 398 274 147 411 5188
Fragments arranged by size:
5188 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2880 2910 1178
Size: 30 165 30 1477 30 4686
Fragments arranged by size:
4686 1477 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2132 2228 2512 2687 3612 1186
Size: 946 96 284 175 925 3992
Fragments arranged by size:
3992 946 925 284 175 96
BseYI C'CCAG_C
Cuts at: 5621 6117 5621
Size: 496 5922
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5845
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3868 4629 5657 1540
Size: 275 186 1867 761 1028 2301
Fragments arranged by size:
2301 1867 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2831 4479 4564 5635 2145
Size: 686 1648 85 1071 2928
Fragments arranged by size:
2928 1648 1071 686 85
BsmI GAATG_Cn'
Cuts at: 3929 4022 3929
Size: 93 6325
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2607 2831 3385 3737 4479 4564
Size: 399 355 462 224 554 352 742 85
Cuts at: 4564 5635 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 554 462 399 355 352
224 85
BspEI T'CCGG_A
Cuts at: 6081 6081
Size: 6418
BspHI T'CATG_A
Cuts at: 2308 3442 4307 6037 2308
Size: 1134 865 1730 2689
Fragments arranged by size:
2689 1730 1134 865
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3773 844
Size: 2929 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4305 1394
Size: 429 2482 3507
Fragments arranged by size:
3507 2482 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3336 3464 4913 5087 65
Size: 1183 2088 128 1449 174 1396
Fragments arranged by size:
2088 1449 1396 1183 174 128
BsrFI r'CCGG_y
Cuts at: 1700 1810 5062 5249 1700
Size: 110 3252 187 2869
Fragments arranged by size:
3252 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6418
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6418
BssSI C'ACGA_G
Cuts at: 2608 2629 4478 5490 2608
Size: 21 1849 1012 3536
Fragments arranged by size:
3536 1849 1012 21
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6418
BstBI TT'CG_AA
Cuts at: 2664 3504 5222 2664
Size: 840 1718 3860
Fragments arranged by size:
3860 1718 840
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6418
BstYI r'GATC_y
Cuts at: 1972 2636 3748 4115 4500 4517 5969 6055 6067
Size: 664 1112 367 385 17 1452 86 12
Cuts at: 6067 6413 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1112 664 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2705 3377 2705
Size: 672 5746
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5679
Fragments arranged by size:
5679 683 56
BtsI GCAGTG_nn'
Cuts at: 3255 3934 4728 4748 3255
Size: 679 794 20 4925
Fragments arranged by size:
4925 794 679 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6418
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6383
DraI TTT'AAA
Cuts at: 3722 3722
Size: 6418
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5425 809
Size: 507 4109 1802
Fragments arranged by size:
4109 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3865 4754 6383
Size: 54 1425 50 275 18 2035 889 1629
Cuts at: 6383 8
Size: 43
Fragments arranged by size:
2035 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3865 1537
Size: 275 2053 4090
Fragments arranged by size:
4090 2053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2325 2844 2963 3345 4348 1353
Size: 972 519 119 382 1003 3423
Fragments arranged by size:
3423 1003 972 519 382 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5005 5379 5525 387
Size: 1064 138 3416 374 146 1280
Fragments arranged by size:
3416 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2143 2143
Size: 6418
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2374 2497 2577 2882 3275 4481 5078
Size: 393 742 123 80 305 393 1206 597
Cuts at: 5078 5865 6133 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1206 787 742 597 393 393 305
268 123 80
EcoO109I rG'GnC_Cy
Cuts at: 2374 2436 2992 3355 2374
Size: 62 556 363 5437
Fragments arranged by size:
5437 556 363 62
EcoRI G'AATT_C
Cuts at: 3727 4105 3727
Size: 378 6040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2677 2677
Size: 6418
FspI TGC'GCA
Cuts at: 4924 4924
Size: 6418
HaeII r_GCGC'y
Cuts at: 1678 5565 1678
Size: 3887 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3119 3151 3526 3558 5059
Size: 32 135 32 1547 32 375 32 1501
Cuts at: 5059 5091 5133 5165 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1547 1501 375 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2679 3762 4008 669
Size: 988 281 741 1083 246 3079
Fragments arranged by size:
3079 1083 988 741 281 246
HindIII A'AGCT_T
Cuts at: 748 3170 748
Size: 2422 3996
HpaI GTT'AAC
Cuts at: 2679 4008 2679
Size: 1329 5089
KpnI G_GTAC'C
Cuts at: 3743 3743
Size: 6418
MfeI C'AATT_G
Cuts at: 3204 4017 3204
Size: 813 5605
MluI A'CGCG_T
Cuts at: 6294 6294
Size: 6418
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2851 5532 5716 1305
Size: 1546 2681 184 2007
Fragments arranged by size:
2681 2007 1546 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4596
Fragments arranged by size:
4596 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2313 3189 4376 4735 4894 518
Size: 648 1147 876 1187 359 159 2042
Fragments arranged by size:
2042 1187 1147 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2164 2517 3310 3472 3628 3834 4511
Size: 419 139 353 793 162 156 206 677
Cuts at: 4511 5659 5904 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 793 677 419 353 245 206
162 156 139
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5866
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6418
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6418
NotI GC'GGCC_GC
Cuts at: 3865 3865
Size: 6418
NsiI A_TGCA'T
Cuts at: 3196 3196
Size: 6418
NspI r_CATG'y
Cuts at: 1132 1229 3091 3776 5321 1132
Size: 97 1862 685 1545 2229
Fragments arranged by size:
2229 1862 1545 685 97
PasI CC'CwG_GG
Cuts at: 1348 2400 1348
Size: 1052 5366
PciI A'CATG_T
Cuts at: 5317 5317
Size: 6418
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6418
PmeI GTTT'AAAC
Cuts at: 3722 3722
Size: 6418
PmlI CAC'GTG
Cuts at: 3700 3700
Size: 6418
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2484 2516 4487 4519 6000 6032 2484
Size: 32 1971 32 1481 32 2870
Fragments arranged by size:
2870 1971 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2374 2436 3355 2374
Size: 62 919 5437
Fragments arranged by size:
5437 919 62
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6418
PsiI TTA'TAA
Cuts at: 3988 3988
Size: 6418
PspOMI G'GGCC_C
Cuts at: 2603 2603
Size: 6418
PstI C_TGCA'G
Cuts at: 830 2130 2499 2644 3770 830
Size: 1300 369 145 1126 3478
Fragments arranged by size:
3478 1300 1126 369 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6418
PvuII CAG'CTG
Cuts at: 2025 2164 3310 3472 2025
Size: 139 1146 162 4971
Fragments arranged by size:
4971 1146 162 139
SacI G_AGCT'C
Cuts at: 2145 2145
Size: 6418
SalI G'TCGA_C
Cuts at: 1936 3760 1936
Size: 1824 4594
SapI GCTCTTCn'nnn_
Cuts at: 2325 2325
Size: 6418
SbfI CC_TGCA'GG
Cuts at: 3770 3770
Size: 6418
ScaI AGT'ACT
Cuts at: 4666 6378 4666
Size: 1712 4706
SfcI C'TryA_G
Cuts at: 826 1045 2126 2495 2640 3152 3766 4901 5582
Size: 219 1081 369 145 512 614 1135 681
Cuts at: 5582 5773 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1081 681 614 512 369 219
191 145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6418
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6418
SmaI CCC'GGG
Cuts at: 1872 3745 1872
Size: 1873 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2302 2501 2747 4522 5423 5685
Size: 197 926 359 199 246 1775 901 262
Cuts at: 5685 820
Size: 1553
Fragments arranged by size:
1775 1553 926 901 359 262 246 199
197
SnaBI TAC'GTA
Cuts at: 493 4209 493
Size: 3716 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6418
SphI G_CATG'C
Cuts at: 1132 3091 3776 1132
Size: 1959 685 3774
Fragments arranged by size:
3774 1959 685
SspI AAT'ATT
Cuts at: 5 52 3634 4342 5
Size: 47 3582 708 2081
Fragments arranged by size:
3582 2081 708 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6418
StyI C'CwwG_G
Cuts at: 513 1065 3857 513
Size: 552 2792 3074
Fragments arranged by size:
3074 2792 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2106 2165 4445 4462 4615 4800 5245
Size: 1255 526 59 2280 17 153 185 445
Cuts at: 5245 325
Size: 1498
Fragments arranged by size:
2280 1498 1255 526 445 185 153 59
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4664 6376 96
Size: 274 80 33 51 1443 2687 1712 138
Fragments arranged by size:
2687 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2865 2880 2897 2912 6378 6410 2865
Size: 15 17 15 3466 32 2873
Fragments arranged by size:
3466 2873 32 17 15 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6418
XbaI T'CTAG_A
Cuts at: 3754 3754
Size: 6418
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6418
XmaI C'CCGG_G
Cuts at: 1870 3743 1870
Size: 1873 4545
XmnI GAAnn'nnTTC
Cuts at: 2660 4547 2660
Size: 1887 4531
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4226 276
Size: 53 83 186 936 2692 2468
Fragments arranged by size:
2692 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI