Restriction map for
pFN21AB9420
(Circular) (Six-base) MAPSORT of: pFN21AB9420.seq Check: 1150 from: 1 to: 6274
pFN21AB9420 6274 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4084 278
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2398 3617 1937
Size: 461 1219 4594
Fragments arranged by size:
4594 1219 461
Acc65I G'GTAC_C
Cuts at: 3063 3595 3063
Size: 532 5742
AclI AA'CG_TT
Cuts at: 1563 4401 4774 1563
Size: 2838 373 3063
Fragments arranged by size:
3063 2838 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3350 3357 3417 4337 5721 5989 1632
Size: 1718 7 60 920 1384 268 1917
Fragments arranged by size:
1917 1718 1384 920 268 60 7
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6077
AflIII A'CryG_T
Cuts at: 1203 3117 3412 5173 6150 1203
Size: 1914 295 1761 977 1327
Fragments arranged by size:
1914 1761 1327 977 295
AgeI A'CCGG_T
Cuts at: 5105 5105
Size: 6274
AhdI GACnn_n'nnGTC
Cuts at: 5003 5003
Size: 6274
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6274
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6274
AlwNI CAG_nnn'CTG
Cuts at: 4017 5589 4017
Size: 1572 4702
ApaLI G'TGCA_C
Cuts at: 2694 4331 5487 2694
Size: 1637 1156 3481
Fragments arranged by size:
3481 1637 1156
ApoI r'AATT_y
Cuts at: 1493 2012 2650 2892 3359 3517 3583 3809 3961
Size: 519 638 242 467 158 66 226 152
Cuts at: 3961 6070 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 638 519 467 242 226 158
152 66
AseI AT'TA_AT
Cuts at: 160 1032 2967 4828 160
Size: 872 1935 1861 1606
Fragments arranged by size:
1935 1861 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
AvaI C'yCGr_G
Cuts at: 1870 1943 3009 3505 3599 1870
Size: 73 1066 496 94 4545
Fragments arranged by size:
4545 1066 496 94 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3047 3080 3047
Size: 33 6241
BamHI G'GATC_C
Cuts at: 3604 3971 3604
Size: 367 5907
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3063 3595 5050 618
Size: 325 217 627 1276 532 1455 1842
Fragments arranged by size:
1842 1455 1276 627 532 325 217
BanII G_rGCy'C
Cuts at: 1391 3593 1391
Size: 2202 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3166 3562 928
Size: 971 1267 396 3640
Fragments arranged by size:
3640 1267 971 396
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4465 4499 1903
Size: 34 2528 34 3678
Fragments arranged by size:
3678 2528 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4166 5376 4166
Size: 1210 5064
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6274
BfrBI ATG'CAT
Cuts at: 2167 2167
Size: 6274
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4885 136
Size: 107 122 71 631 605 3213 1525
Fragments arranged by size:
3213 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6269 6269
Size: 6274
BlpI GC'TnA_GC
Cuts at: 3691 3691
Size: 6274
Bme1580I G_kGCm'C
Cuts at: 1790 2698 4335 5491 1790
Size: 908 1637 1156 2573
Fragments arranged by size:
2573 1637 1156 908
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6274
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4005 4963 442
Size: 416 53 83 399 24 2588 958 1753
Fragments arranged by size:
2588 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2993 1056
Size: 1937 4337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4934 1239
Size: 3695 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4399 5264 5562 4399
Size: 865 298 5111
Fragments arranged by size:
5111 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2212 2535 4937 882
Size: 617 713 323 2402 2219
Fragments arranged by size:
2402 2219 713 617 323
BsaAI yAC'GTr
Cuts at: 493 1204 2900 4065 493
Size: 711 1696 1165 2702
Fragments arranged by size:
2702 1696 1165 711
BsaBI GATnn'nnATC
Cuts at: 2472 6268 2472
Size: 3796 2478
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4081 4463 5098 275
Size: 53 83 186 936 2548 382 635 1451
Fragments arranged by size:
2548 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4707 5105 5379 5526 5937 4707
Size: 398 274 147 411 5044
Fragments arranged by size:
5044 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2943 2973 1178
Size: 30 165 30 1540 30 4479
Fragments arranged by size:
4479 1540 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2951 3278 1186
Size: 1765 327 4182
Fragments arranged by size:
4182 1765 327
BseYI C'CCAG_C
Cuts at: 5477 5973 5477
Size: 496 5778
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2686 3182 1163
Size: 573 950 496 4255
Fragments arranged by size:
4255 950 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3724 4485 5513 1540
Size: 275 186 1723 761 1028 2301
Fragments arranged by size:
2301 1723 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2698 4335 4420 5491 2698
Size: 1637 85 1071 3481
Fragments arranged by size:
3481 1637 1071 85
BsmI GAATG_Cn'
Cuts at: 2167 3785 3878 2167
Size: 1618 93 4563
Fragments arranged by size:
4563 1618 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2160 2160
Size: 6274
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2698 3593 4335 4420 5491 1391
Size: 399 908 895 742 85 1071 2174
Fragments arranged by size:
2174 1071 908 895 742 399 85
BspEI T'CCGG_A
Cuts at: 5937 5937
Size: 6274
BspHI T'CATG_A
Cuts at: 2296 2856 4163 5893 2296
Size: 560 1307 1730 2677
Fragments arranged by size:
2677 1730 1307 560
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3629 844
Size: 2785 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4161 1394
Size: 429 2338 3507
Fragments arranged by size:
3507 2338 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2210 2268 4769 4943 65
Size: 1183 962 58 2501 174 1396
Fragments arranged by size:
2501 1396 1183 962 174 58
BsrFI r'CCGG_y
Cuts at: 1700 1810 3066 4918 5105 1700
Size: 110 1256 1852 187 2869
Fragments arranged by size:
2869 1852 1256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6274
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6274
BssSI C'ACGA_G
Cuts at: 4334 5346 4334
Size: 1012 5262
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6274
BstBI TT'CG_AA
Cuts at: 3424 3514 5078 3424
Size: 90 1564 4620
Fragments arranged by size:
4620 1564 90
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6274
BstXI CCAn_nnnn'nTGG
Cuts at: 2342 2342
Size: 6274
BstYI r'GATC_y
Cuts at: 1972 2192 2409 2670 3604 3971 4356 4373 5825
Size: 220 217 261 934 367 385 17 1452
Cuts at: 5825 5911 5923 6269 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 934 385 367 346 261 220
217 86 17 12
BstZ17I GTA'TAC
Cuts at: 2399 2399
Size: 6274
BtgI C'CryG_G
Cuts at: 513 1065 3183 513
Size: 552 2118 3604
Fragments arranged by size:
3604 2118 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5535
Fragments arranged by size:
5535 683 56
BtsI GCAGTG_nn'
Cuts at: 2441 3790 4584 4604 2441
Size: 1349 794 20 4111
Fragments arranged by size:
4111 1349 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6274
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6239
DraI TTT'AAA
Cuts at: 2427 2739 3578 2427
Size: 312 839 5123
Fragments arranged by size:
5123 839 312
DraIII CAC_nnn'GTG
Cuts at: 2228 2228
Size: 6274
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5281 809
Size: 507 3965 1802
Fragments arranged by size:
3965 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3068 3242 3721
Size: 54 1425 50 275 18 1238 174 479
Cuts at: 3721 4610 6239 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1238 889 479 275 174 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3721 1537
Size: 275 1909 4090
Fragments arranged by size:
4090 1909 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3393 4204 1353
Size: 2040 811 3423
Fragments arranged by size:
3423 2040 811
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4861 5235 5381 387
Size: 1064 138 3272 374 146 1280
Fragments arranged by size:
3272 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3350 3357 3417 4337 4934 5721 5989
Size: 393 1718 7 60 920 597 787 268
Cuts at: 5989 1239
Size: 1524
Fragments arranged by size:
1718 1524 920 787 597 393 268 60
7
EcoO109I rG'GnC_Cy
Cuts at: 3502 3502
Size: 6274
EcoRI G'AATT_C
Cuts at: 3583 3961 3583
Size: 378 5896
EcoRV GAT'ATC
Cuts at: 2474 2474
Size: 6274
FspI TGC'GCA
Cuts at: 4780 4780
Size: 6274
HaeII r_GCGC'y
Cuts at: 1678 5421 1678
Size: 3743 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2482 2514 4915 4947 4989
Size: 32 135 32 910 32 2401 32 42
Cuts at: 4989 5021 1373
Size: 32 2626
Fragments arranged by size:
2626 2401 910 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3618 3864 669
Size: 988 281 1680 246 3079
Fragments arranged by size:
3079 1680 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2337 2594 748
Size: 1589 257 4428
Fragments arranged by size:
4428 1589 257
HpaI GTT'AAC
Cuts at: 3864 3864
Size: 6274
KpnI G_GTAC'C
Cuts at: 3067 3599 3067
Size: 532 5742
MfeI C'AATT_G
Cuts at: 3873 3873
Size: 6274
MluI A'CGCG_T
Cuts at: 6150 6150
Size: 6274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2174 3235 5388 5572 1305
Size: 869 1061 2153 184 2007
Fragments arranged by size:
2153 2007 1061 869 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3244 10
Size: 54 1425 343 1412 3040
Fragments arranged by size:
3040 1425 1412 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2261 2861 4232 4591 4750 518
Size: 648 1095 600 1371 359 159 2042
Fragments arranged by size:
2042 1371 1095 648 600 359 159
MspA1I CmG'CkG
Cuts at: 1606 3395 3690 4367 5515 5760 1606
Size: 1789 295 677 1148 245 2120
Fragments arranged by size:
2120 1789 1148 677 295 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5722
NdeI CA'TA_TG
Cuts at: 387 2611 387
Size: 2224 4050
NheI G'CTAG_C
Cuts at: 1052 2989 1052
Size: 1937 4337
NotI GC'GGCC_GC
Cuts at: 3721 3721
Size: 6274
NsiI A_TGCA'T
Cuts at: 2169 2169
Size: 6274
NspI r_CATG'y
Cuts at: 1132 1229 2747 3121 3416 3449 3568 3632 5177
Size: 97 1518 374 295 33 119 64 1545
Cuts at: 5177 1132
Size: 2229
Fragments arranged by size:
2229 1545 1518 374 295 119 97 64
33
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6274
PciI A'CATG_T
Cuts at: 3117 3412 5173 3117
Size: 295 1761 4218
Fragments arranged by size:
4218 1761 295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6274
PfoI T'CCnGG_A
Cuts at: 3172 3172
Size: 6274
PmeI GTTT'AAAC
Cuts at: 3578 3578
Size: 6274
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3214 3246 4343 4375 5856 5888 3214
Size: 32 1097 32 1481 32 3600
Fragments arranged by size:
3600 1481 1097 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3502 3502
Size: 6274
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6274
PsiI TTA'TAA
Cuts at: 3844 3844
Size: 6274
PstI C_TGCA'G
Cuts at: 830 2452 3626 830
Size: 1622 1174 3478
Fragments arranged by size:
3478 1622 1174
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6274
PvuII CAG'CTG
Cuts at: 3395 3395
Size: 6274
SalI G'TCGA_C
Cuts at: 1936 3616 1936
Size: 1680 4594
SbfI CC_TGCA'GG
Cuts at: 3626 3626
Size: 6274
ScaI AGT'ACT
Cuts at: 3101 4522 6234 3101
Size: 1421 1712 3141
Fragments arranged by size:
3141 1712 1421
SfcI C'TryA_G
Cuts at: 826 1045 2373 2448 3622 4757 5438 5629 826
Size: 219 1328 75 1174 1135 681 191 1471
Fragments arranged by size:
1471 1328 1174 1135 681 219 191 75
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6274
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6274
SmaI CCC'GGG
Cuts at: 1872 3507 3601 1872
Size: 1635 94 4545
Fragments arranged by size:
4545 1635 94
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4378 5279 5541 820
Size: 197 926 2435 901 262 1553
Fragments arranged by size:
2435 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4065 493
Size: 3572 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6274
SphI G_CATG'C
Cuts at: 1132 3568 3632 1132
Size: 2436 64 3774
Fragments arranged by size:
3774 2436 64
SspI AAT'ATT
Cuts at: 5 52 3471 4198 5
Size: 47 3419 727 2081
Fragments arranged by size:
3419 2081 727 47
StuI AGG'CCT
Cuts at: 1517 2146 1517
Size: 629 5645
StyI C'CwwG_G
Cuts at: 513 1065 2245 3713 513
Size: 552 1180 1468 3074
Fragments arranged by size:
3074 1468 1180 552
SwaI ATTT'AAAT
Cuts at: 2427 2427
Size: 6274
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3337 4301 4318 4471 4656 5101 325
Size: 1255 1757 964 17 153 185 445 1498
Fragments arranged by size:
1757 1498 1255 964 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2832 3099
Size: 274 80 33 51 1443 207 648 267
Cuts at: 3099 3417 4520 6232 96
Size: 318 1103 1712 138
Fragments arranged by size:
1712 1443 1103 648 318 274 267 207
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2943 2975 3168 3200 6234 6266 2943
Size: 32 193 32 3034 32 2951
Fragments arranged by size:
3034 2951 193 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6274
XbaI T'CTAG_A
Cuts at: 3610 3610
Size: 6274
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2897 2897
Size: 6274
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6274
XmaI C'CCGG_G
Cuts at: 1870 3505 3599 1870
Size: 1635 94 4545
Fragments arranged by size:
4545 1635 94
XmnI GAAnn'nnTTC
Cuts at: 3520 4403 3520
Size: 883 5391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4082 276
Size: 53 83 186 936 2548 2468
Fragments arranged by size:
2548 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI Bsu36I EcoICRI EcoNI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI