Restriction map for
pFN21AB9415
(Circular) (Six-base) MAPSORT of: pFN21AB9415.seq Check: 8912 from: 1 to: 6775
pFN21AB9415 6775 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4585 278
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2323 4118 1937
Size: 386 1795 4594
Fragments arranged by size:
4594 1795 386
Acc65I G'GTAC_C
Cuts at: 3972 4096 3972
Size: 124 6651
AclI AA'CG_TT
Cuts at: 1563 4902 5275 1563
Size: 3339 373 3063
Fragments arranged by size:
3339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2049 2957 3189 4838 6222 6490 1632
Size: 417 908 232 1649 1384 268 1917
Fragments arranged by size:
1917 1649 1384 908 417 268 232
AflII C'TTAA_G
Cuts at: 820 1017 3938 820
Size: 197 2921 3657
Fragments arranged by size:
3657 2921 197
AflIII A'CryG_T
Cuts at: 1203 2111 2397 5674 6651 1203
Size: 908 286 3277 977 1327
Fragments arranged by size:
3277 1327 977 908 286
AgeI A'CCGG_T
Cuts at: 5606 5606
Size: 6775
AhdI GACnn_n'nnGTC
Cuts at: 5504 5504
Size: 6775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6775
AlwNI CAG_nnn'CTG
Cuts at: 3679 4518 6090 3679
Size: 839 1572 4364
Fragments arranged by size:
4364 1572 839
ApaLI G'TGCA_C
Cuts at: 3361 4832 5988 3361
Size: 1471 1156 4148
Fragments arranged by size:
4148 1471 1156
ApoI r'AATT_y
Cuts at: 1493 2013 2153 2335 2673 2861 2921 3225 3343
Size: 520 140 182 338 188 60 304 118
Cuts at: 3343 3609 4084 4310 4462 6571 1493
Size: 266 475 226 152 2109 1697
Fragments arranged by size:
2109 1697 520 475 338 304 266 226
188 182 152 140 118 60
AseI AT'TA_AT
Cuts at: 160 1032 2595 5329 160
Size: 872 1563 2734 1606
Fragments arranged by size:
2734 1606 1563 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
AvaI C'yCGr_G
Cuts at: 1870 1943 2492 4100 1870
Size: 73 549 1608 4545
Fragments arranged by size:
4545 1608 549 73
BamHI G'GATC_C
Cuts at: 4105 4472 4105
Size: 367 6408
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3972 4096 5551 618
Size: 325 217 627 2185 124 1455 1842
Fragments arranged by size:
2185 1842 1455 627 325 217 124
BanII G_rGCy'C
Cuts at: 1391 4094 1391
Size: 2703 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4011 928
Size: 971 2112 3692
Fragments arranged by size:
3692 2112 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4966 5000 1903
Size: 34 3029 34 3678
Fragments arranged by size:
3678 3029 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3068 4667 5877 3068
Size: 1599 1210 3966
Fragments arranged by size:
3966 1599 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3215 3282 4041
Size: 107 122 71 631 605 1543 67 759
Cuts at: 4041 5386 136
Size: 1345 1525
Fragments arranged by size:
1543 1525 1345 759 631 605 122 107
71 67
BglII A'GATC_T
Cuts at: 2950 6770 2950
Size: 3820 2955
BlpI GC'TnA_GC
Cuts at: 3163 3622 4192 3163
Size: 459 570 5746
Fragments arranged by size:
5746 570 459
Bme1580I G_kGCm'C
Cuts at: 1790 3365 4836 5992 1790
Size: 1575 1471 1156 2573
Fragments arranged by size:
2573 1575 1471 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6775
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4506 5464 442
Size: 416 53 83 399 24 3089 958 1753
Fragments arranged by size:
3089 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2813 1056
Size: 1757 5018
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2903 3760 3966 2903
Size: 857 206 5712
Fragments arranged by size:
5712 857 206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2913 5435 1239
Size: 1674 2522 2579
Fragments arranged by size:
2579 2522 1674
Bpu10I CC'TnA_GC
Cuts at: 2466 2466
Size: 6775
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2292 4050 4900 5765 6063 2292
Size: 1758 850 865 298 3004
Fragments arranged by size:
3004 1758 865 850 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2158 5438 882
Size: 617 659 3280 2219
Fragments arranged by size:
3280 2219 659 617
BsaAI yAC'GTr
Cuts at: 493 1204 4566 493
Size: 711 3362 2702
Fragments arranged by size:
3362 2702 711
BsaBI GATnn'nnATC
Cuts at: 6769 6769
Size: 6775
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4582 4964 5599 275
Size: 53 83 186 936 3049 382 635 1451
Fragments arranged by size:
3049 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5208 5606 5880 6027 6438 5208
Size: 398 274 147 411 5545
Fragments arranged by size:
5545 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3833 3863 1178
Size: 30 165 30 2430 30 4090
Fragments arranged by size:
4090 2430 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3644 1186
Size: 2458 4317
BseYI C'CCAG_C
Cuts at: 2962 3515 3546 5978 6474 2962
Size: 553 31 2432 496 3263
Fragments arranged by size:
3263 2432 553 496 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6202
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4225 4986 6014 1540
Size: 275 186 2224 761 1028 2301
Fragments arranged by size:
2301 2224 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3365 4836 4921 5992 3365
Size: 1471 85 1071 4148
Fragments arranged by size:
4148 1471 1071 85
BsmI GAATG_Cn'
Cuts at: 4286 4379 4286
Size: 93 6682
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3365 4094 4836 4921 5992 1391
Size: 399 1575 729 742 85 1071 2174
Fragments arranged by size:
2174 1575 1071 742 729 399 85
BspEI T'CCGG_A
Cuts at: 6438 6438
Size: 6775
BspHI T'CATG_A
Cuts at: 4664 6394 4664
Size: 1730 5045
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4130 844
Size: 3286 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 3849 4662 1394
Size: 429 1798 228 813 3507
Fragments arranged by size:
3507 1798 813 429 228
BsrDI GCAATG_nn'
Cuts at: 65 1248 5270 5444 65
Size: 1183 4022 174 1396
Fragments arranged by size:
4022 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2144 5419 5606 1700
Size: 110 334 3275 187 2869
Fragments arranged by size:
3275 2869 334 187 110
BsrGI T'GTAC_A
Cuts at: 96 2182 96
Size: 2086 4689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6775
BssSI C'ACGA_G
Cuts at: 2888 4835 5847 2888
Size: 1947 1012 3816
Fragments arranged by size:
3816 1947 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3047 794
Size: 2253 4522
BstBI TT'CG_AA
Cuts at: 5579 5579
Size: 6775
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6775
BstXI CCAn_nnnn'nTGG
Cuts at: 3932 3932
Size: 6775
BstYI r'GATC_y
Cuts at: 1972 2224 2582 2950 3299 3325 4105 4472 4857
Size: 252 358 368 349 26 780 367 385
Cuts at: 4857 4874 6326 6412 6424 6770 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 780 385 368 367 358 349
346 252 86 26 17 12
BstZ17I GTA'TAC
Cuts at: 2324 2324
Size: 6775
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5284
Fragments arranged by size:
5284 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6036
Fragments arranged by size:
6036 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 4291 5085 5105 3157
Size: 1134 794 20 4827
Fragments arranged by size:
4827 1134 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6740
DraI TTT'AAA
Cuts at: 2667 4079 2667
Size: 1412 5363
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5782 809
Size: 507 4466 1802
Fragments arranged by size:
4466 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4222 5111 6740
Size: 54 1425 50 275 18 2392 889 1629
Cuts at: 6740 8
Size: 43
Fragments arranged by size:
2392 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4222 1537
Size: 275 2410 4090
Fragments arranged by size:
4090 2410 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3292 4705 1353
Size: 1939 1413 3423
Fragments arranged by size:
3423 1939 1413
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2872 5362 5736 5882 387
Size: 1064 138 1283 2490 374 146 1280
Fragments arranged by size:
2490 1283 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2049 2913 2957 3189 4838 5435 6222
Size: 393 417 864 44 232 1649 597 787
Cuts at: 6222 6490 1239
Size: 268 1524
Fragments arranged by size:
1649 1524 864 787 597 417 393 268
232 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2085 2085
Size: 6775
EcoO109I rG'GnC_Cy
Cuts at: 2884 2884
Size: 6775
EcoRI G'AATT_C
Cuts at: 2335 3343 4084 4462 2335
Size: 1008 741 378 4648
Fragments arranged by size:
4648 1008 741 378
FspI TGC'GCA
Cuts at: 5281 5281
Size: 6775
HaeII r_GCGC'y
Cuts at: 1678 5922 1678
Size: 4244 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2352 2384 3106 3138 5416
Size: 32 135 32 780 32 722 32 2278
Cuts at: 5416 5448 5490 5522 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2278 780 722 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2460 2613 4119 4365 669
Size: 988 281 522 153 1506 246 3079
Fragments arranged by size:
3079 1506 988 522 281 246 153
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6775
HpaI GTT'AAC
Cuts at: 2613 4365 2613
Size: 1752 5023
KpnI G_GTAC'C
Cuts at: 3976 4100 3976
Size: 124 6651
MfeI C'AATT_G
Cuts at: 3051 3093 4374 3051
Size: 42 1281 5452
Fragments arranged by size:
5452 1281 42
MluI A'CGCG_T
Cuts at: 6651 6651
Size: 6775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3674 5889 6073 1305
Size: 2369 2215 184 2007
Fragments arranged by size:
2369 2215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4953
Fragments arranged by size:
4953 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4733 5092 5251 518
Size: 648 3567 359 159 2042
Fragments arranged by size:
3567 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3206 3435 3676 4191 4868 6016 6261 1606
Size: 1600 229 241 515 677 1148 245 2120
Fragments arranged by size:
2120 1600 1148 677 515 245 241 229
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5284
Fragments arranged by size:
5284 939 552
NdeI CA'TA_TG
Cuts at: 387 2059 387
Size: 1672 5103
NheI G'CTAG_C
Cuts at: 1052 2809 1052
Size: 1757 5018
NotI GC'GGCC_GC
Cuts at: 4222 4222
Size: 6775
NspI r_CATG'y
Cuts at: 1132 1229 2115 2281 2401 2911 3203 4133 5678
Size: 97 886 166 120 510 292 930 1545
Cuts at: 5678 1132
Size: 2229
Fragments arranged by size:
2229 1545 930 886 510 292 166 120
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6775
PciI A'CATG_T
Cuts at: 2111 2397 5674 2111
Size: 286 3277 3212
Fragments arranged by size:
3277 3212 286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3012 1108
Size: 1904 4871
PmeI GTTT'AAAC
Cuts at: 4079 4079
Size: 6775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4844 4876 6357 6389 4844
Size: 32 1481 32 5230
Fragments arranged by size:
5230 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2884 2884
Size: 6775
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6775
PsiI TTA'TAA
Cuts at: 4345 4345
Size: 6775
PstI C_TGCA'G
Cuts at: 830 4127 830
Size: 3297 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6775
PvuII CAG'CTG
Cuts at: 3206 3676 3206
Size: 470 6305
SalI G'TCGA_C
Cuts at: 1936 4117 1936
Size: 2181 4594
SapI GCTCTTCn'nnn_
Cuts at: 3292 3292
Size: 6775
SbfI CC_TGCA'GG
Cuts at: 4127 4127
Size: 6775
ScaI AGT'ACT
Cuts at: 5023 6735 5023
Size: 1712 5063
SfcI C'TryA_G
Cuts at: 826 1045 2760 2802 4123 5258 5939 6130 826
Size: 219 1715 42 1321 1135 681 191 1471
Fragments arranged by size:
1715 1471 1321 1135 681 219 191 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 4041 4041
Size: 6775
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6775
SmaI CCC'GGG
Cuts at: 1872 4102 1872
Size: 2230 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2307 3938 4065 4879 5780 6042
Size: 197 926 364 1631 127 814 901 262
Cuts at: 6042 820
Size: 1553
Fragments arranged by size:
1631 1553 926 901 814 364 262 197
127
SnaBI TAC'GTA
Cuts at: 493 4566 493
Size: 4073 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6775
SphI G_CATG'C
Cuts at: 1132 2281 4133 1132
Size: 1149 1852 3774
Fragments arranged by size:
3774 1852 1149
SspI AAT'ATT
Cuts at: 5 52 3664 4699 5
Size: 47 3612 1035 2081
Fragments arranged by size:
3612 2081 1035 47
StuI AGG'CCT
Cuts at: 1517 3429 1517
Size: 1912 4863
StyI C'CwwG_G
Cuts at: 513 1065 2004 4214 513
Size: 552 939 2210 3074
Fragments arranged by size:
3074 2210 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3307 4802 4819 4972 5157 5602 325
Size: 1255 1727 1495 17 153 185 445 1498
Fragments arranged by size:
1727 1498 1495 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2182 5021 6733
Size: 274 80 33 51 1443 205 2839 1712
Cuts at: 6733 96
Size: 138
Fragments arranged by size:
2839 1712 1443 274 205 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3688 3720 6735 6767 3688
Size: 32 3015 32 3696
Fragments arranged by size:
3696 3015 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6775
XbaI T'CTAG_A
Cuts at: 3454 3878 4111 3454
Size: 424 233 6118
Fragments arranged by size:
6118 424 233
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3126 3126
Size: 6775
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6775
XmaI C'CCGG_G
Cuts at: 1870 4100 1870
Size: 2230 4545
XmnI GAAnn'nnTTC
Cuts at: 2013 4904 2013
Size: 2891 3884
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4583 276
Size: 53 83 186 936 3049 2468
Fragments arranged by size:
3049 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfoI SrfI SwaI